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Citations for Millipore Sigma :
1 - 50 of 10000+ citations for Norovirus GI.1 VLP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... the bound NoV capsid proteins were detected with mouse anti norovirus GII (1:1000; NativeAntigen) and by adding horseradish peroxidase conjugated with goat anti-mouse IgG (1:1500; Sigma Aldrich). Horseradish peroxidase activity was detected with a TMB (3,3’,5,5’-tetramethylbenzidine ...
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bioRxiv - Molecular Biology 2022Quote: ... with the selective ADAM10 inhibitor GI254023X (GI) at 1 μM (SML0789, Sigma) or Dimethyl sulfoxide (DMSO ...
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bioRxiv - Molecular Biology 2022Quote: ... with the selective ADAM10 inhibitor GI254023X (GI) at 1 μM (SML0789, Sigma) or Dimethyl sulfoxide (DMSO ...
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bioRxiv - Immunology 2021Quote: The naturally packaged ssRNA in Qβ-VLPs was digested as following: Qβ(ssRNA)-VLPs were washed 3x with 20mM HEPES (Sigma-Aldrich) using Amicon ultra 0.5ml tubes 100kDa MWCO ...
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bioRxiv - Microbiology 2023Quote: ... VLP and cells were stained with SYBRGreen I (Sigma) for 10 min in the dark at 80°C and let cool down for 5 min at room temperature [46] ...
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bioRxiv - Cancer Biology 2021Quote: ... supplemented with 1 μM GW280264X (GW) (Generon, AOB3632-5) or GI254023X (GI) (Sigma, SML0789-5MG) when indicated ...
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bioRxiv - Cell Biology 2019Quote: ... Supernatants containing VLPs were filtered (Millex HV, 0.45 μm; Millipore) and frozen at −80°C until use ...
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bioRxiv - Microbiology 2022Quote: ... The VLP pellet was suspended in 1 mL SM buffer with lysozyme (10.0 mg/reaction; Sigma Aldrich) and incubated for 60 min at 37 °C with gentle shaking to degrade unfiltered bacterial cells ...
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bioRxiv - Evolutionary Biology 2022Quote: ... VLP pellets were lysed in 60 μl of NP-40 lysis buffer containing 1% NP-40 (Sigma-Aldrich) and 10 μg/ml each of aprotinin ...
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bioRxiv - Microbiology 2023Quote: ... The VLPs were concentrated with Polyethylene glycol 8000 (Sigma-Aldrich, Cat#P2139), overnight precipitation and purification by chloroform treatment ...
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bioRxiv - Immunology 2022Quote: ... or MDM (106) were treated for 1h at 37°C with 1 ml of VLPs-VPX+ containing 5 μg/ml polybrene (Sigma, #H9268) for SAMHD1 degradation16,37 before the addition of 20 µg CAp24 of each control or/and encoding-gene lentiviral vectors with 5 μg/ml polybrene for 2h transduction ...
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bioRxiv - Synthetic Biology 2019Quote: ... plates were coated with indicated concentrations of VLP (4°C, over-night) and subsequently blocked with 1% casein in PBS (Sigma-Aldrich) for 2h at 37°C before primary antibodies were added (polyclonal mouse anti-Pfs230 or monoclonal 1B3 antibody ...
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bioRxiv - Molecular Biology 2021Quote: ... All lentivectors and VLPs were DNAse treated (2 h at 37°C, DNaseI, Sigma) before they were concentrated by ultracentrifugation in a Sorvall Discovery (Hitachi ...
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bioRxiv - Microbiology 2023Quote: ... We collected VLP bands and buffer exchanged using a 100-kDa Amicon filter (Sigma). VLPs were buffer exchanged into DPBS (Thermo Fisher Scientific Cat # ...
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bioRxiv - Molecular Biology 2020Quote: ... The GABAB-Gi-SCFV16 complex was concentrated in a 100-kDa cutoff Vivaspin (Millipore) filter and run on a Superose™ 6 Increase column.
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bioRxiv - Cell Biology 2024Quote: The A Disintegrin and Metalloprotease (ADAM)-10 inhibitor GI 254023X was from Sigma-Aldrich Canada (SML0789 ...
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bioRxiv - Microbiology 2021Quote: ... VLP-containing supernatant was filtered through a 0.2 um sterile syringe filter (Millex-GP, Millipore Sigma, USA) to remove the remaining bacteria ...
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bioRxiv - Microbiology 2024Quote: ... and VLPs were concentrated 10 times by the addition of PBS/8 % (w/v) PEG 6000 (Sigma), incubation at 4° C for 24 h ...
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bioRxiv - Microbiology 2021Quote: ... The VLP-containing supernatant was filtered through a 0.2 μm sterile syringe filter (Millex-GP, Millipore Sigma, USA) concentrated by centrifugation (35,000g ...
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bioRxiv - Immunology 2021Quote: ... Qβ(1668)-VLPs were then derivatized for 30min at RT using dibenzocyclooctyne NHS ester (DBCO cross-linker) (Sigma-Aldrich) in a 5-fold molar excess ...
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bioRxiv - Biophysics 2020Quote: ... and the presence of ZTL variants and HA-GI was detected by western blot using anti-FLAG (Sigma) and anti-HA (3F10 ...
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bioRxiv - Microbiology 2020Quote: ... VLP samples were separated by 10% or 14% SDS-PAGE later blotted to PVDF membrane (Immobilon®-FL, Merck Millipore) and blocked overnight with ODYSSEY® blocking buffer ...
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bioRxiv - Immunology 2020Quote: ... all CIS43 VLPs and wild-type (WT) Qβ VLPs were depleted of endotoxin by three rounds of phase separation using Triton X-114 (Sigma-Aldrich), as described in reference 48 ...
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bioRxiv - Biophysics 2019Quote: ... Purified PCV2 VLPs were concentrated and buffer exchanged to phosphate buffered saline (PBS) using Amicon Utra-4 centrifugal filter devices (Merck Millipore, MA). PCV2 VLP samples were stored in 50-100 µl aliquots at −80°C.
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bioRxiv - Molecular Biology 2023Quote: ... ChAC-VLPs was further concentrated to 5 times of its initial volume using 100 kDa cut-off concentrator (Merck, Millipore, USA) followed by analysis on 10% Tris-Glycine SDS-PAGE and successful RBD-E2 fusion protein is validated by western blot using anti-RBD antibody.
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bioRxiv - Microbiology 2023Quote: ... VLPs were lysed by the addition of 100 μl of Phage Lysis Buffer (4.5 M guanidinium isothiocyanate (Sigma-Aldrich Cat#50983), 44 mM sodium citrate pH 7.0 (Sigma-Aldrich Cat#C8532) ...
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bioRxiv - Microbiology 2023Quote: ... The VLPs were subsequently concentrated down to 180 µL using Amicon Ultra-15 100K Centrifugal Filter Units (Milipore Sigma, MO, U.S.A). To remove any free contaminating nucleic acids ...
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The intestinal circadian clock drives microbial rhythmicity to maintain gastrointestinal homeostasisbioRxiv - Physiology 2022Quote: Complete GI transit time (GITT) was measured by administering natural carmine red (6%, SigmaAldrich) dissolved in 0.5% methylcellulose (Sigma-Aldrich) by gavage (100ul ...
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bioRxiv - Biophysics 2020Quote: ... The supernatant was filtered using 0.2um filter and VLPs were captured in a 40-10% sucrose step gradient and concentrated using AMICON spin filters (UFC901008, EMD Millipore, Burlington, MA). Total protein yield for purified VLP stock varied somewhat but was typically above 5 mg/mL ...
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bioRxiv - Microbiology 2020Quote: ... The colon samples were then cut lengthwise over ice and the faecal matter was removed and the remaining GI tissue was then rolled using the ‘Swiss roll technique’ before embedding in 2.5% medium viscosity carboxymethyl cellulose (Sigma-Aldrich, Dorset, UK). Both brains and colons were sectioned using a CM3050S cryostat microtome (Leica Biosystems ...
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bioRxiv - Developmental Biology 2021Quote: To measure cell cycle occupancy we stained replicate RetCFP/+ and RetCFP/CFP whole GI tracts from both male and female mice at E14.5 for phosphohistone H3 (Anti-H3S10p; Millipore Sigma #06-570), an immunomarker specific to cells undergoing mitosis (Skaland et al. ...
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bioRxiv - Plant Biology 2022Quote: ... detached leaves were incubated for 30 min in lactophenol Trypan Blue solution (water: glycerol: lactic acid: phenol [1:1:1:1] + Trypan Blue solution [1 mg mL-1; Sigma-Aldrich]). Thereafter ...
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bioRxiv - Physiology 2023Quote: ... 1 mg·ml−1 trypsin inhibitor and 1 mg·ml−1 bovine serum albumin (Sigma, UK) at 37°C for 45 minutes ...
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bioRxiv - Molecular Biology 2021Quote: ... Necrostatin-1 (Nec-1, Sigma), GSK872 (a gift from Anaxis Pty ...
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bioRxiv - Molecular Biology 2020Quote: ... Endothelin-1 (ET-1, Millipore), isoproterenol hydrochloride (Iso ...
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bioRxiv - Cell Biology 2020Quote: ... CASPAR-1 (Sigma, 1:500) and GAPDH (Cell Signaling ...
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bioRxiv - Cell Biology 2022Quote: ... 1:1 Nutrient Mixture (Sigma), 1X N2 supplement (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... 1 g·L-1 metronidazole (Sigma) and 0.5 g·L-1 vancomycin (Macklin) ...
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bioRxiv - Microbiology 2021Quote: ... 1 g·L-1 neomycin (Sigma), 1 g·L-1 metronidazole (Sigma ...
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bioRxiv - Developmental Biology 2019Quote: ... 1% glutamax and 1% anti-anti) at a 1:1 ratio and neutralized using 1 M NaOH (Sigma-Aldrich). The neutralized ECM solution was mixed with 3 mg/mL rat tail collagen type I (Corning ...
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bioRxiv - Physiology 2022Quote: ... ZO-1 (WB 1:500, IF 1:50, Millipore, AB2272), α-catenin (WB 1:500 ...
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bioRxiv - Immunology 2021Quote: The S-variant and RBD coupled bead sets were incubated with sera from immunized mice at 4 dilutions (1:100; 1:1000; 1:10000; 1:100000) for 1 h in 96-well filter plates (MultiScreenHTS; Millipore) and analyzed on a Luminex analyzer (Luminex / R&D Systems ...
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bioRxiv - Developmental Biology 2021Quote: ... α-tubulin (1:100, 1:500 cells, 1:1000 tissue, Sigma, T9026), ΔNp63 (1:100 ...
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bioRxiv - Developmental Biology 2021Quote: ... Rabbit anti-Drosophila caspase-1 (DCP-1) (1:100) (Sigma-Aldrich); rat anti-Elav (1:100 ...
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bioRxiv - Neuroscience 2023Quote: ... secondary antibodies (1:1000) and DAPI (D9542-1, Sigma, 1:10,000) were incubated for 2hrs at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... TRA-1-60 (Millipore, 1:200), MAP2 (Synaptic System ...
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bioRxiv - Microbiology 2021Quote: ... 1 µg.mL−1 DAPI (Sigma-Aldrich) was added to the cells and incubated for 5 min at room temperature prior to centrifugation ...
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bioRxiv - Bioengineering 2022Quote: ... 1 μg mL-1 hydrocortisone (Sigma), 0.2 mM ascorbic acid 2-phosphate (49752 ...
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bioRxiv - Cell Biology 2022Quote: ... Tuj-1 (1:500, T5076, Sigma), Ac-Tub (1:500 ...
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bioRxiv - Cell Biology 2022Quote: ... Liproxstatin-1 (Lip-1, Sigma-Aldrich), a-Tocopherol (MedChemExpress) ...