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Citations for Millipore Sigma :
1 - 50 of 10000+ citations for Mouse G Protein Coupled Bile Acid Receptor 1 GPBAR1 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Bile acid concentration in each sample was quantified using the Bile Acid Assay Kit (Sigma #MAK309) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2022Quote: Total bile acids in feces was measured using Bile Acid Assay Kit (Sigma-Aldrich MAK309, Merck, France). The L-lactate concentration was measured using a L-lactate Assay Kit (Sigma-Aldrich MAK329 ...
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bioRxiv - Cancer Biology 2019Quote: ... Plasma concentration of non-sulfated bile acids were measured with the Bile Acid Assay Kit (Sigma-Aldrich) using a Fluoroskan Ascent fluorescence multiwell plate reader (Thermo Labsystems) ...
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bioRxiv - Microbiology 2023Quote: ... Bile acids were purchased from Sigma-Aldrich. GUDCA ...
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bioRxiv - Developmental Biology 2022Quote: ... Sepharose coupled with protein G (Sigma) was blocked with 5% BSA (Sigma ...
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bioRxiv - Physiology 2021Quote: ... t-BA were measured utilizing the fluorimetric bile acid assay kit (Sigma, #MAK309) according to the manufacturer’s protocol.
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bioRxiv - Microbiology 2024Quote: ... DMEM with 0.1-10% physiologic bile acids (B8381, Sigma-Aldrich), or DMEM control media ...
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bioRxiv - Physiology 2023Quote: ... mouse GLP-1 ELISA kit (EZGLP1T) from Sigma-Aldrich; mouse GDF15 ELISA kit (DY6385 ...
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bioRxiv - Physiology 2024Quote: ... kynurenic acid 1 (nonspecific glutamate receptor antagonist, Sigma-Aldrich). Coronal slices from mouse somatosensory cortex (300μm thick ...
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bioRxiv - Neuroscience 2019Quote: ... four bile acids (glycochenodeoxycholic acid [GCDCA], taurocholic acid [TCA], taurodeoxycholic acid [TDCA] and glycocholic acid [GCA]; Sigma Aldrich, Munich, Germany) and four amino acids (Trp ...
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bioRxiv - Biochemistry 2020Quote: ... Immunoprecipitations using antibodies coupled to protein G-Sepharose beads (Sigma) were as described previously (Levy et al. ...
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bioRxiv - Microbiology 2020Quote: ... we re-suspended 20 g of sample in 200 ml anaerobic peptone wash (1 g/l peptone, 0.5 g/l L-Cysteine, 0.5 g/l bile salts and 0.001 g/l Resazurin; Sigma-Aldrich) to prepare a 10% (w/v ...
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bioRxiv - Microbiology 2022Quote: ... Different amounts of specific bile salts [taurocholic acid (Sigma Aldrich 86339-25G), glycocholic acid (Sigma Aldrich G7132-1G) ...
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bioRxiv - Microbiology 2019Quote: ... two media containing either commercialized mixed bile salts (0.5 g L−1, 1:1 sodium cholate: sodium deoxycholate, cat# 48305, Sigma-Aldrich) or a primary bile salts composition (0.25 g L−1 sodium cholate and 0.25 g L−1 sodium chenodeoxycholate ...
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bioRxiv - Microbiology 2022Quote: ... 1 mg/mL bile salts (Sigma-Aldrich), and 40 mM NaHCO3 ...
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bioRxiv - Biochemistry 2021Quote: ... bile acids and the other analytes were obtained from Sigma-Aldrich (Steinheim, Germany); SCB ...
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bioRxiv - Microbiology 2022Quote: ... or MRS broth with the addition of 0.3% sterilized bovine bile acids (Sigma), respectively ...
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bioRxiv - Biochemistry 2022Quote: ... 50µL of a suspension of Protein G coupled to agarose beads (Sigma) previously equilibrated in Bax buffer (20mM Tris/HCl pH 8.0 ...
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bioRxiv - Developmental Biology 2020Quote: ... We used 10µl G-protein coupled Sepharose beads per IP (SIGMA, P3296). Anti-Myc IPs were performed with 10µl Anti-Myc-Agarose bead (SIGMA ...
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bioRxiv - Microbiology 2024Quote: ... followed by protein G coupled magnetic beads at room temperature for 10 minutes (PureProteome Protein G Magnetic Bead System, Millipore LSKMAGG). The beads were removed using a magnetic rack ...
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bioRxiv - Plant Biology 2022Quote: ... Bile extract (Sigma-B8631; 8.5 mg ml-1) and pancreatin (Sigma-P1750 ...
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bioRxiv - Physiology 2021Quote: ... mouse FGF21 ELISA kit (Sigma-Aldrich, EZRMFGF21-26K).
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bioRxiv - Neuroscience 2023Quote: ... anti-mouse IL-6 ELISA kit (Sigma, RAB0309), anti-mouse G-CSF ELISA kit (MCS00 ...
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bioRxiv - Microbiology 2023Quote: ... Bile-supplemented cultures contained 0.5% purified dehydrated ox bile (Millipore-Sigma) in LB unless otherwise specified ...
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bioRxiv - Microbiology 2023Quote: ... Bile-supplemented cultures contained 0.5% purified dehydrated ox bile (Millipore-Sigma) in LB unless otherwise specified ...
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bioRxiv - Physiology 2020Quote: ... according to the manufacturer’s instructions (Mouse Leptin ELISA Kit, 90030; Crystal Chem, Zaandam, Netherlands; Rat/Mouse Insulin ELISA Kit, cat. EZRMI-13K; Merck Millipore). The intra- and interassay coefficients of variation (CVs ...
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bioRxiv - Microbiology 2021Quote: ... The soluble fraction was then added to the Protein G sepharose beads coupled to either HA 12CA5 mouse monoclonal IgG (Sigma Aldrich) or anti-Ty1 (BB2 ...
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bioRxiv - Developmental Biology 2022Quote: ... FLAG-tagged constructs were then immunoprecipitated with Dynabeads Protein G beads coupled to mouse anti-FLAG M2 mAb (Sigma #F3165, RRID:AB_259529) for 3 hours at 4°C with rotation ...
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bioRxiv - Neuroscience 2021Quote: ... were diluted to a final concentration of 10-4 M and bile acid (TDCA; Sigma) was diluted to 10-5 M in ACSF immediately before the experiment ...
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Vitamin D signaling orchestrates skeletal muscle metabolic flexibility by regulating its fuel choicebioRxiv - Physiology 2023Quote: ... Serum insulin levels were determined by ELISA using mouse insulin ELISA kit from Sigma according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2021Quote: ... was prepared using 3N HCl and 3N NaOH and four different bile concentrations (0% control, 0.3%, 0.5% and 1%) was prepared using ox bile extract (Sigma, Germany) followed by incubation at 36.5°C for 30 minutes to activate oxyrase ...
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bioRxiv - Microbiology 2021Quote: ... Porcine bile (Sigma Aldrich) was dissolved in sterile BHI with streptomycin to ensure sterility ...
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bioRxiv - Microbiology 2019Quote: ... Bile salts (Sigma B8756) were used at a concentration of 0.4% w/v ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit (rb)-α-gamma-aminobutyric acid receptor α6 (GABARα6; 1:500; Millipore Sigma; #AB5610), rb-α-T-box brain protein 2 (Tbr2 ...
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bioRxiv - Biochemistry 2023Quote: ... and probe the receptor using anti-FLAG peroxidase coupled antibody (1:2000, Sigma, Cat. no. A8592). Data were quantified using ImageLab software (Bio-Rad ...
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bioRxiv - Developmental Biology 2021Quote: ... The remaining supernatant was incubated with Protein G-coupled magnetic beads (Sigma, MO, USA) that captured with anti-GFP for 4 h ...
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bioRxiv - Bioengineering 2022Quote: ... supernatant protein was removed by acidification with 5 g L-1 trichloroacetic acid (Supelco from Sigma-Aldrich), incubation for 20 min in room temperature and centrifugation at 20,238 x g for 10 min in a table-top centrifuge (Eppendorf 5424 ...
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bioRxiv - Immunology 2023Quote: ... Brilliant Blue G (BBG, P2X7 receptor antagonist, 1μM, Sigma), AP-5 (25 nM ...
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bioRxiv - Cell Biology 2021Quote: Mouse pancreatic islets were isolated via bile duct collagenase digestion (Collagenase P, Sigma) and Biocoll (Millipore ...
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bioRxiv - Physiology 2024Quote: ... mouse pancreata were perfused through the common bile duct with Collagenase P (Sigma) and islets were purified by density gradient centrifugation using Histopaque 1.077 (Sigma) ...
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bioRxiv - Microbiology 2023Quote: ... Cleared cell lysates were immunoprecipitated using 1 μg FLAG-M2 antibody coupled to 40 μL of packed protein G Sepharose beads (Cat GE17-0618-01, Sigma) for 3 h at 4°C ...
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bioRxiv - Neuroscience 2021Quote: ... the glutamate receptor antagonist kynurenic acid (4 mM; Sigma– Aldrich) was locally applied in the pretectum by pressure injection through a micropipette fixed to a holder (containing Fast Green to aid visualization of the injection spread) ...
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bioRxiv - Neuroscience 2022Quote: ... and anti-glutamate receptor 2 (mouse anti-GluR2 IgG2a; Millipore; 1:2000). Sections were then washed with PBS and incubated in Alexa Fluor-conjugated fluorescent secondary antibodies (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... Stock solutions of all bile acids were prepared by dissolving compounds in molecular biology-grade DMSO (Sigma). These solutions were used to establish standard curves ...
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bioRxiv - Neuroscience 2024Quote: ... anti-mouse HRP-coupled (1:10 000, AP181P, Sigma), anti-rabbit HRP-coupled (1:10 000 ...
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bioRxiv - Physiology 2020Quote: ... and each circulating hormones were determined using a commercially available ELISA kit for total ghrelin (Rat/Mouse Total Ghrelin ELISA kit, EZRGRT-91K, Millipore), and active ghrelin (Rat/Mouse Total Ghrelin ELISA kit ...
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bioRxiv - Cell Biology 2024Quote: The Protein-G CoIP kit (IP50, Sigma Aldrich) was used for coimmunoprecipitation (CoIP ...
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bioRxiv - Biochemistry 2022Quote: ... 0.3 g of glass beads (acid washed, 1 mm, Sigma) were added and the cells were disrupted in a MiniBeadbeater-24 (Bio Spec Products Inc. ...
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bioRxiv - Microbiology 2023Quote: ... bile salts were supplied from bovine bile and measured using a commercial kit according to the supplier’s protocol (Sigma-Aldrich, cat. No. MAK 309). Finally ...
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bioRxiv - Neuroscience 2023Quote: ... 0,005 CPP [()-3-(2-carboxypiperazin-4-yl)propyl-1-phosphonic acid] (NMDA receptors antagonist, Sigma Aldrich). Traces of whole-cell voltage-clamp recordings (holding potential ...