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Citations for Millipore Sigma :
1 - 36 of 36 citations for Methotrexate SAP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... methotrexate (Sigma) was dissolved in DMSO to make 5 mM stock solution ...
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bioRxiv - Cell Biology 2021Quote: ... Methotrexate (Sigma, A6770) was used in high folate media to inhibit the folate pathway ...
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bioRxiv - Cancer Biology 2019Quote: ... methotrexate (Sigma #A6770), 6-mercaptopurine (Sigma #852678) ...
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bioRxiv - Bioengineering 2022Quote: ... 100 nM methotrexate (Sigma-Aldrich), and 0.2% anti-clumping agent (Thermo Fisher Scientific ...
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Tracing production instability in a clonally-derived CHO cell line using single cell transcriptomicsbioRxiv - Cell Biology 2021Quote: ... Methotrexate (300 nM; Sigma-Aldrich M9929) was added to the culture medium every third passage to maintain stability of anti-IL8 gene expression ...
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bioRxiv - Cancer Biology 2020Quote: ... Methotrexate was purchased from Sigma Aldrich.
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bioRxiv - Bioengineering 2021Quote: ... and 200 nM Methotrexate (MTX, SIGMA). CHO-K1 cells (ATCC ...
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bioRxiv - Bioengineering 2021Quote: ... and 200 nM Methotrexate (MTX, Sigma).
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bioRxiv - Systems Biology 2023Quote: Methotrexate (Sigma Aldrich/Merck, Darmstadt, Germany)
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bioRxiv - Biophysics 2021Quote: ... The pellet was resuspended and we isolated DHFR by methotrexate-affinity chromatography using methotrexate-agarose (Sigma-Aldrich) followed by anion-exchange chromatography using a HiPrep Q FF column (Cytiva) ...
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bioRxiv - Cell Biology 2019Quote: ... Methotrexate (M9929) was purchased from Sigma-Aldrich and prepared by dissolving in PBS to a concentration of 1 mg/ml ...
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bioRxiv - Biochemistry 2019Quote: ... and methotrexate were purchased from Sigma Aldrich. Sulfasalazine was dissolved in 50 mM NaHCO3 and methotrexate was dissolved in 100 mM NaHCO3 ...
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bioRxiv - Microbiology 2020Quote: ... and methotrexate (MTX) were purchased from Sigma and were dissolved in 100% DMSO (Sigma) ...
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bioRxiv - Cell Biology 2023Quote: ... Drugs were commercially purchased: methotrexate (Sigma-Aldrich).
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bioRxiv - Cancer Biology 2023Quote: ... or seed saporin (SAP) (Sigma-Aldrich) in 1 M Na2CO3 (pH 11.5 ...
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bioRxiv - Immunology 2021Quote: ... methotrexate (MTX, Sigma Aldrich M9929, 1mg/kg every other day), 6a-methylprednisolone (MP ...
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bioRxiv - Cell Biology 2024Quote: Mice received bilateral injections of either unconjugated blank-SAP or SAP conjugated to an antibody directed to DBH (DBH-SAP; 42 ng/200 nL, pH=7.4; MAB394; Millipore, Temecula, CA, USA), using a Hamilton syringe directed toward the nTS (0.1 mm rostral to Obex ...
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bioRxiv - Bioengineering 2022Quote: MRX-2843 (obtained under MTA from Meryx Inc.) and methotrexate (Sigma-Aldrich) were reconstituted in dry DMSO and stored in checkerboard array within 384-well polypropylene plates (VWR ...
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bioRxiv - Cell Biology 2021Quote: ... Methotrexate and sodium orthovanadate (SOV) were purchased from Sigma Aldrich (St. Louis, MO). Dulbecco’s modified eagle medium (DMEM ...
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bioRxiv - Biophysics 2021Quote: Anti-β-tubulin antibodies (75 μg ml−1, SAP.4G5, Sigma-Aldrich) in BRB80 was flushed into the flow cell and incubated for 5 min to allow for hydrophobic interaction-based absorption to the surface ...
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bioRxiv - Immunology 2021Quote: ... the wells were incubated with streptavidin-alkaline phosphatase (SAP) (Sigma Aldrich, USA) for one hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... mouse monoclonal anti-β-Tubulin antibody (1:10,000; SAP.4G5 Sigma-Aldrich); rabbit monoclonal anti-TBP (1:2000 ...
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bioRxiv - Biochemistry 2021Quote: ... Protein was eluted in buffer B with 0.5 M KCl and 1 mM methotrexate (Sigma). Then the protein was precipitated with 40% TCA for 60 minutes on ice followed by centrifugation (10000 g ...
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bioRxiv - Biochemistry 2023Quote: ... a final concentration of 100 μg/mL methotrexate (Sigma-Aldrich, 100 mM stock in DMSO) and 1 mM sulfanilamide (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2019Quote: ... washed with PBS and permeabilized with SAP buffer [0.2% Saponin (Sigma-Aldrich S4521), 0.2% Gelatin (Himedia Laboratories TC041 ...
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bioRxiv - Molecular Biology 2024Quote: ... The dorsal cuticle (with nephrocytes) was transferred to methotrexate solution (10 μM; 06563, Sigma-Aldrich, MO) in Schneider’s Drosophila Medium (Thermo Fisher Scientific ...
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bioRxiv - Plant Biology 2019Quote: ... the filtered inflorescence sap was directly used as reducing agent to synthesize AgNPs using AgNO3 (SIGMA) as precursor ...
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bioRxiv - Microbiology 2020Quote: ... The microbial DNA was purified using the Exo-sap DNA Clean-Up Kit (Sigma-Aldrich, Israel) using 5 L aliquot of the supernatant ...
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bioRxiv - Biochemistry 2021Quote: ... and the soluble fraction tumbled end-over-end for 2 hours with 15 µL (bed volume) of methotrexate-agarose (Sigma). Then the beads were washed once in 1 mL of buffer B with 0.5 M KCl ...
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bioRxiv - Biophysics 2020Quote: ... filled and incubated successively with anti β-tubulin I (monoclonal antibody SAP.4G5 from Sigma in PEM), Pluronic F-127 (1 % in PEM) ...
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bioRxiv - Biophysics 2020Quote: ... filled and incubated successively with anti β-tubulin I (monoclonal antibody SAP.4G5 from Sigma in PEM) for 15-20 min ...
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bioRxiv - Cell Biology 2021Quote: ... single cell clones were generated by serial dilution in 96-well plates and maintained in growth media with 200 μg/mL Zeocin and 5 μM methotrexate (MTX, EMD Millipore). CHO-SEAP CMV::XRCC6 ATM+ PRKDC+ was generated analogously to CHO-K1 ATM+ PRKDC+ ...
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bioRxiv - Biochemistry 2023Quote: ... The cells were resuspended in 500 μL sterile water and each replicate was plated on a BioAssay dish (245mm × 245mm) containing SC+leucine+methionine+histidine medium with 100 μg/mL methotrexate (Sigma-Aldrich) and 1 mM sulfanilamide (Sigma-Aldrich) ...
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bioRxiv - Plant Biology 2021Quote: Interactions of shikonin with cell sap was analyzed by normal phase TLC (silica gel 60 F254, Merck Millipore, Darmstadt, Germany). In addition ...
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bioRxiv - Cancer Biology 2023Quote: ... the unencapsulated FITC-dextran or FITC-SAP was removed by washing and filtration using Amicon Ultra centrifugal filters (100 K device, Merck Millipore). The incorporation of FITC-dextran and FITC-SAP into the nsEVs was confirmed by spectrofluometer analysis (FP-6200 ...
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bioRxiv - Neuroscience 2024Quote: ... aCSF and 192IgG-SAP groups received intraperitoneal (ip) injections of WIN55,212-2 (0.5 mg/kg or 3 mg/kg) (Sigma-Aldrich, St. Louis, MO, USA) or vehicle solution (1:1:18 ...