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Citations for Millipore Sigma :
1 - 50 of 10000+ citations for MBL 2 MBP C Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... containing 2% (vol/vol) of heat inactivated (HI, for 1h at 56°C) human AB serum (hABS) (Sigma, #H3667). After extensive washings with DPBS (Gibco ...
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bioRxiv - Immunology 2021Quote: Expression cassette of maltose binding protein (MBP)-Mb2Cas12a-His in pMal vector was transformed to Rosetta 2 (DE3) (Novagen) competent cells ...
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bioRxiv - Biochemistry 2022Quote: Human embryonic kidney 293 cells (HEK293) (Sigma), HEK293T (TakaraBio) ...
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bioRxiv - Neuroscience 2020Quote: ... Cultured human embryonic kidney 293 (HEK293) cells were transfected using 2 μg/μl polyethylenimine (Sigma-Aldrich) with indicated plasmids for the expression of either Flag-KCTDs alone or in combination with Flag-Cav2.3 ...
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bioRxiv - Neuroscience 2021Quote: Human embryonic kidney (HEK293) cells were maintained in a 37°C/5% CO2 atmosphere in DMEM:F12 (Sigma, D6421) supplemented with 10% foetal bovine serum (ClonTech ...
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bioRxiv - Biophysics 2020Quote: ... MBP and the His-tag were cleaved from the P domain using HRV 3C protease (Novagen). Cleaved P domain protein eluted from Ni-NTA resin and was further purified by size-exclusion chromatography using a Superdex 16/600 200 pg column (GE Healthcare ...
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bioRxiv - Neuroscience 2019Quote: ... and His-tagged human HDAC6 protein (EMD Millipore) were incubated with 57 nm 32P-ATP in kinase buffer (50 mM Tris HCl ...
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bioRxiv - Cell Biology 2024Quote: HEK293 (Human Embryonic Kidney, ECACC 96121229 from Sigma-Aldrich Chemie GmbH) were cultured in DMEM (Dulbecco’s modified Eagle’s medium PAN biotech ...
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bioRxiv - Microbiology 2022Quote: ... The human bNAb VRC01 and MBP-specific mouse antibody (Sigma, catalog no. M6295-.2ML) were diluted in blocking buffer to a maximum concentration of 1 μg/ml and to reach ×466 dilution (as recommended by the manufacturer) ...
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bioRxiv - Neuroscience 2019Quote: ... MBP (Millipore Sigma; catalog # AB980 ...
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bioRxiv - Neuroscience 2022Quote: ... chicken-MBP (Millipore), guinea pig-IBA1 (Synaptic Systems) ...
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bioRxiv - Neuroscience 2023Quote: ... MBP (MAB386, Millipore) diluted in TBST with 2% BSA overnight (BP9706-100 ...
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bioRxiv - Neuroscience 2023Quote: ... anti-MBP (Millipore, Billerica ...
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bioRxiv - Synthetic Biology 2021Quote: ... cerevisiae were cloned into 10×His-MBP-SUMO-nup-SNAP constructs (Figure 2A) via a pET-28a-derived vector (Novagen), and expressed in E ...
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bioRxiv - Biochemistry 2021Quote: ... After removal of the His-tagged MBP by Re-IMAC the unbound material was concentrated in 50 kDa centrifugal concentrators (Millipore) and subjected to SEC on a Superdex 200 Increase column (equilibrated 150 mM NaCl ...
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bioRxiv - Molecular Biology 2019Quote: ... was fused with an N-terminal His(6)-tag followed by maltose binding protein (MBP) tag and TEV cleavage site in pET21 vector (Novagen).
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bioRxiv - Microbiology 2020Quote: ... was cloned as 10× His-MBP-SUMO-NUP153 (or NSP1)-SNAP constructs (Figure S1) into a pET-28a-derived vector (Novagen) and expressed in BL21-Gold (DE3) ...
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bioRxiv - Cell Biology 2021Quote: ... LC3B (Sigma, L7543; MBL International, PM036), TRIM5 (Cell Signaling #14326) ...
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bioRxiv - Microbiology 2022Quote: ... and HEK293-ACE2 cells [HEK293 cells (ATCC CRL-1573) stably expressing human ACE2]22 were maintained in DMEM (high glucose) (Sigma-Aldrich, Cat# 6429-500ML) containing 10% FBS ...
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bioRxiv - Neuroscience 2019Quote: ... was incubated with recombinant His-tagged human HDAC6 protein (EMD Millipore) in 250 μl RB100 buffer (25 mM HEPES pH 7.5 ...
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bioRxiv - Neuroscience 2022Quote: ... RRID:AB_839504) and myelin basic protein (MBP; rat anti-MBP; #MAB386, Millipore; RRID:AB_94975).
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bioRxiv - Physiology 2020Quote: ... and human C-peptide (Millipore) were detected by ELISA kits in the plasma samples collected during the human insulin or human C-peptide challenges ...
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bioRxiv - Neuroscience 2022Quote: ... MBP (Millipore #13–110), PGK1 or CREBRF were the substrates used for the kinase assays 29.
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bioRxiv - Molecular Biology 2024Quote: HEK293 and Caco-2 cells were grown in DMEM culturing medium (Sigma) with 10% FBS (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... was added to the cell pellet and stored at −20°C prior to being assayed for insulin using human insulin specific RIA (Millipore Cat# HI-14K).
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bioRxiv - Biochemistry 2022Quote: ... and another was loaded with 10 ng of human HSP70 (His tagged human HSP70, SRP5190, Millipore Sigma), which allowed comparisons to be made among separate gels ...
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bioRxiv - Neuroscience 2022Quote: ... Blots were blocked with 5% BSA in Tween-TBS at room temperature for 2 h and incubated at 4 °C overnight with anti-His antibody (1:200; Sigma Aldrich) in blocking buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... MBP (EMD Millipore, 1:1,000) or GalC (EMD Millipore ...
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bioRxiv - Neuroscience 2021Quote: ... anti-MBP (1:500, Millipore) and anti-LacZ (1:2,000 ...
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bioRxiv - Neuroscience 2021Quote: ... Degraded MBP (Sigma-Aldrich, AB5864).
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bioRxiv - Microbiology 2020Quote: ... rabbit polyclonal α-MBP (Sigma), or mouse monoclonal α-RpoA (Biolegend ...
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bioRxiv - Neuroscience 2022Quote: ... MBP (MAB386, Millipore, 1:300) and SPARCL1 (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... MBP (1:1000, Millipore, MAB382), Olig2 (1:1000 ...
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bioRxiv - Neuroscience 2021Quote: ... MBP (1:1000, Millipore, MAB382), ErbB3 (1:200 ...
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bioRxiv - Neuroscience 2019Quote: ... anti-MBP (1:500, Millipore) and anti-LacZ (1:2,000 ...
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bioRxiv - Neuroscience 2021Quote: ... MBP (1:100, Millipore, MAB382), TCF4 (1:500 ...
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Phosphorylation of the Smooth Muscle Master Splicing Regulator RBPMS Regulates its Splicing ActivitybioRxiv - Molecular Biology 2022Quote: ... Myelin Basic Protein (MBP; Millipore) was used as a positive control ...
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bioRxiv - Neuroscience 2023Quote: ... MBP (1:100, Millipore, MAB382), ASPA (1:500 ...
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bioRxiv - Neuroscience 2023Quote: ... MBP (1:1000, Millipore, MAB382), Olig2 (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... anti-MBP (Millipore, AB980,1:500). Secondary antibodies include ...
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bioRxiv - Neuroscience 2024Quote: ... anti-MBP rat (Millipore MAB386), anti-CC1 mouse (Calbiochem OP80) ...
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bioRxiv - Neuroscience 2019Quote: ... and PAK/cofilin mechanism experiments used human embryonic kidney cells (HEK293) cultured in DMEM:F12 (Sigma, D6421) supplemented with 10% foetal bovine serum (ClonTech ...
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bioRxiv - Cancer Biology 2023Quote: ... 2% heat-inactivated Fetal Bovine Serum (HI-FBS; Sigma-Aldrich), 1% v/v penicillin-Streptomycin (VWR) ...
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bioRxiv - Plant Biology 2023Quote: ... in-frame of the C-terminal (His)6-tag (Novagen, http://www.emdbiosciences.com) resulting in pET28a:ApTPS1 ...
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bioRxiv - Biophysics 2021Quote: His-tagged human PCNA protein was purchased from Sigma Aldrich (catalogue number SRP5117), at a concentration of 6.6 μM ...
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bioRxiv - Molecular Biology 2021Quote: ... 500 µg of protein was incubated overnight at 4°C with primary antibody as indicated (anti-AHCY [MBL RN126PW], anti-HA [Millipore 05-904] ...
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bioRxiv - Cell Biology 2020Quote: ... Primary antibodies used were: α-CENP-C (MBL:1:1000) as a kinetochore marker and α-tubulin (Sigma: 1:500). Samples were incubated in primary antibody solution for 1 hour at 37 °C ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... thereafter referred to as Mpp75Aa1.1-His) or without the C-terminal His tag (thereafter referred as Mpp75Aa1.1) was cloned into a pET24a vector (Novagen, Madison, WI) and expressed in Rosetta 2 (DE3 ...
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bioRxiv - Neuroscience 2022Quote: ... 2% Ara-C (Sigma) in 0.9% saline or saline alone was infused directly in the lateral ventricle (ipsilateral side ...
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bioRxiv - Cell Biology 2023Quote: ... with 2% human serum (Sigma), then labelled with APC-conjugated anti-mouse CD31 antibody ...