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Citations for Millipore Sigma :
1 - 50 of 10000+ citations for M CSF Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... 25 ng/mL M-CSF (Sigma Aldrich) was added and maintained in the media and after week 2 ...
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bioRxiv - Bioengineering 2022Quote: ... 1% penicillin/streptomycin and 30ng/mL of M-CSF (Sigma Aldrich) and subsequently plated at a density of 4×105 cells/mL ...
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bioRxiv - Immunology 2022Quote: ... Macrophage colony-stimulating factor (M-CSF, human) was purchased from Millipore Sigma, St ...
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bioRxiv - Cancer Biology 2023Quote: ... were used to generate conditioned medium with M-CSF (Sigma Aldrich, 85011425). Cells were cultured at 37°C and 5% CO2 in a sterile environment ...
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bioRxiv - Microbiology 2021Quote: ... Primary human monocytes were treated with 50 ng/ml M-CSF (Sigma-Aldrich) in complete RPMI for a week before infection ...
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bioRxiv - Microbiology 2023Quote: ... and 30 ng/mL macrophage-colony stimulating factor (M-CSF) (Sigma-Aldrich®: M6518), replacing the medium every third day17,18 ...
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Domain-specific quantification of prion protein in cerebrospinal fluid by targeted mass spectrometrybioRxiv - Neuroscience 2019Quote: ... 30 µL of CSF was incubated with 0.03% CHAPS with 6 M urea (Sigma U0631) and 20 mM TCEP (Pierce 77720 ...
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bioRxiv - Microbiology 2023Quote: ... anti-His (mouse, 1:3k, Sigma, A7058), anti-c-Myc (mouse ...
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bioRxiv - Microbiology 2020Quote: ... Mouse anti-His antibody (1:12000) (Sigma-Aldrich) and biotinylated PNA (1:6000 ...
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bioRxiv - Neuroscience 2021Quote: ... M-CSF differentiated Macrophages were lifted by incubation for 10 minutes at 37°C with Accumax (Sigma). Cells were counted using Muse® Cell Analyzer (Merk Millipore ...
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bioRxiv - Cell Biology 2022Quote: ... mouse α-His-HRP (A7058, Sigma-Aldrich, 1:10000).
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bioRxiv - Cell Biology 2020Quote: ... cells were then plated 0.8 × 105 cell per well in 24 well plates in RPMI medium supplemented with 30 ng/ml M-CSF and with the L-enantiomers amino acids Lys (0.4 mM) and Arg (0.8 mM) (SIGMA) non-isotope-labeled (L amino acids) ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies used for protein quantification for ELISAs were mouse monoclonal anti-His (His-Tag mAb, EMD-Millipore), and biotinylated mouse anti-rat Cd4 (clone OX68) ...
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bioRxiv - Neuroscience 2021Quote: ... MAP2 (Sigma M-1406, mouse, 1:600), NeuN (Millipore MAB377 ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were detected using α -His from mouse (Sigma H1029), α-Gfp from mouse ...
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bioRxiv - Cell Biology 2021Quote: ... Rose-John), 20 U/ml recombinant murine GM-CSF (PharmedArtis, Germany) and 10−6 M dexamethasone (Sigma-Aldrich, USA). Progenitor cells were amplified 5 to 11 days before differentiation into DC was induced by supplementing 200 U/ml recombinant murine GM-CSF ...
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bioRxiv - Cell Biology 2019Quote: ... and 100 ng/ml M-CSF for 24 h and then selected for expression of the construct with 3 μg/ml puromycin (Sigma) for 24 h ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-His antibody (Sigma; 1:5,000 in PBS + 2% BSA) was added and incubated for 1h ...
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bioRxiv - Biochemistry 2021Quote: HEK293 cells (Sigma:85120602) were cultured using DMEM (Gibco™ ...
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bioRxiv - Biophysics 2023Quote: HEK293 cells (Sigma Aldrich) and were cultured in DMEM (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... and 1x non-essential amino acid) and 50 ng/mL of human macrophage colony-stimulating factor (M-CSF, Millipore, Temecula, CA) for 5-6 days at 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... CD14+ macrophages differentiated for 7 days in M-CSF (described above) were detached from the petri dish using 2 mM ethylenediaminetetraacetic acid (EDTA, Sigma Aldrich) in PBS at 4 °C as well as mechanical scraping ...
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bioRxiv - Neuroscience 2023Quote: ... this media was supplemented with 25 ng/mL cytokine M-CSF (Macrophage colony-stimulating factor 1) (Sigma, Catalog No. 300-25), 100 ng/mL IL-34 (Interleukin-34 ...
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bioRxiv - Microbiology 2021Quote: ... Mouse monoclonal (M)Ab against β-actin (Sigma, A1978) was included as a loading control ...
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bioRxiv - Immunology 2022Quote: ... Mouse IgG1 Isotype control (Sigma M-1398, clone MOPC31c), mouse anti-rat CD49d (Thermo Fisher MA49D7 ...
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bioRxiv - Cancer Biology 2023Quote: ... mouse anti-P-MAPK (1:2000, Sigma #M-8159), rabbit anti-MAPK (1:500 ...
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bioRxiv - Microbiology 2021Quote: ... and extensively dialysed against HEPES-buffered saline and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag mAb, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (Bethyl).
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bioRxiv - Cell Biology 2022Quote: ... and extensively dialysed against HBS and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag monoclonal antibody, 70796, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (A3562 ...
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bioRxiv - Biophysics 2023Quote: ... then incubated with mouse anti-His tag primary antibody (Millipore Sigma SAB1305538) at 1:1,000 dilution ...
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bioRxiv - Microbiology 2022Quote: ... with the following modifications: Mouse anti-GST tag or mouse anti-His tag 1:200× (Sigma-Aldrich) and HRP-conjugated goat anti-mouse IgG 1:2,000× (Seracare Life Sciences ...
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bioRxiv - Immunology 2022Quote: ... BMDMs were generated by culturing marrow cells in poly-L-lysine coated culture plate for 7 days with 50 ng/ml M-CSF in RPMI 1640 (Sigma-Aldrich, R8758) supplemented with 20% FBS ...
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bioRxiv - Immunology 2022Quote: ... BLaER1 cells were trans-differentiated for 5-6 day in medium containing 10 ng/mL IL-3, 10 ng/mL M-CSF (MPI of Biochemistry, Munich) and 100 nM β-estradiol (Sigma-Aldrich) at a concentration of 7 × 104 cells/well in 96 well plates ...
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bioRxiv - Cell Biology 2024Quote: ... respectively (Millipore Cat# HI-14K, HI-13K). Samples treated with 3 mM glucose KRBH were measured undiluted ...
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bioRxiv - Biochemistry 2021Quote: HEK293 and HEK293T cells (Sigma) were cultured at 37 °C ...
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bioRxiv - Biochemistry 2022Quote: ... S5H recombinant protein was examined by using specific anti-His mouse antibody (Novagen) as described in (López-Gresa et al. ...
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bioRxiv - Biochemistry 2020Quote: ... The production of the (His)6-tagged recombinant proteins was confirmed by Western analysis using mouse monoclonal α-His-horseradish peroxidase conjugated antibodies (Sigma Aldrich, USA). Imaging of the protein bands on the Western blot was conducted using the ECL kit (ThermoScientific ...
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bioRxiv - Microbiology 2022Quote: 20 µL of a 2.5 µg/mL solution of an affinity purified His-tagged RBD in 0.1 M NaHCO3 pH 9.8 was coated onto Nunc® MaxiSorp™ 384-well plates (Sigma) and incubated overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... bound proteins were detected by immunoblotting with primary mouse anti-His (Sigma□Aldrich, Taiwan) or anti-GST primary antibodies (Sigma□Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... and dilutions were as follows (in parenthesis): His (mouse, Sigma cat# H1029; 1:2,500); β-actin (rabbit ...
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bioRxiv - Microbiology 2022Quote: ... HEK293 WT (Sigma Cat. Nr. 85120602), HEK293 GALNT1 KO (Narimatsu et al. ...
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bioRxiv - Immunology 2021Quote: ... HEK293 cells (Sigma Aldrich 12022001-1VL) were maintained in DMEM supplemented with L-glutamine and 10% FBS ...
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bioRxiv - Immunology 2022Quote: ... Non-specific binding sites were blocked using fluorescence-activated cell sorting (FACS) buffer (1× PBS, 2% hi-FBS) supplemented with 20% hi-FBS and 10 μg mouse IgG (Sigma-Aldrich, St-Louis, MO, USA). Cells were stained using the following conjugated mouse anti-human monoclonal antibodies (mAbs) ...
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bioRxiv - Molecular Biology 2019Quote: ... His-GFP or His-fusion proteins were bound to HIS-Selec HF Nickel Affinity Gel (Sigma) for 1 hour ...
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bioRxiv - Microbiology 2021Quote: ... served as blocking buffer and monoclonal mouse-derived anti-HIS antibody (LS-C57341, LsBio) and HRP conjugated goat anti-mouse IgG antibody (AP308P, Sigma) served as primary and secondary antibodies ...
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bioRxiv - Microbiology 2022Quote: ... These were prepared by preincubating the His-tagged PilC with mouse monoclonal anti-poly-histidine and biotinylated anti-mouse IgG antibodies (both from Sigma) at a ratio of 1:1.5:1.5 (by weight ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Mouse anti-FLAG M2 monoclonal antibody (F1804) and mouse anti-His-tag monoclonal antibody (D291-3S) were purchased from Sigma□Aldrich (St ...
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bioRxiv - Molecular Biology 2020Quote: ... GM-CSF (Sigma-Aldrich, St. Louis, MO). Fibroblasts were monitored for YFP ...
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bioRxiv - Microbiology 2020Quote: ... Blotting was performed using a mouse monoclonal anti-6x His antibody (1:1000; Millipore HIS.H8) and a goat anti-mouse IgG HRP conjugated secondary antibody (1:2000) ...
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bioRxiv - Microbiology 2020Quote: ... anti-mouse (BioSupplies) or anti-His (CBM) secondary antibodies conjugated to alkaline phosphatase (Sigma Aldrich) as described in Pedersen et al35 ...
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bioRxiv - Bioengineering 2021Quote: ... then probed with the conjugated anti-6x His tag® mouse monoclonal antibody (Sigma-Aldrich) overnight at 4ºC or 1 hour at room temperature and rewashed three times with washing buffer (CMT ...