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Citations for Millipore Sigma :
1 - 50 of 437 citations for Japanese Encephalitis Virus VLP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2020Quote: Anti-Japanese encephalitis E (mouse monoclonal, Millipore), influenza A H9N2 HA (mouse monoclonal ...
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bioRxiv - Immunology 2021Quote: The naturally packaged ssRNA in Qβ-VLPs was digested as following: Qβ(ssRNA)-VLPs were washed 3x with 20mM HEPES (Sigma-Aldrich) using Amicon ultra 0.5ml tubes 100kDa MWCO ...
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bioRxiv - Microbiology 2023Quote: ... VLP and cells were stained with SYBRGreen I (Sigma) for 10 min in the dark at 80°C and let cool down for 5 min at room temperature [46] ...
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bioRxiv - Cell Biology 2019Quote: ... Supernatants containing VLPs were filtered (Millex HV, 0.45 μm; Millipore) and frozen at −80°C until use ...
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bioRxiv - Microbiology 2023Quote: ... The VLPs were concentrated with Polyethylene glycol 8000 (Sigma-Aldrich, Cat#P2139), overnight precipitation and purification by chloroform treatment ...
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bioRxiv - Microbiology 2022Quote: ... were purchased from the Japanese Collection of Research Bioresources Cell Bank (Osaka, Japan) and cultured in Dulbecco’s modified Eagle’s medium (DMEM; Sigma Aldrich) supplemented with 5% fetal bovine serum and G418 (Nacalai Tesque ...
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bioRxiv - Immunology 2022Quote: ... Virus was concentrated from media using PEG Virus Precipitation Kit (Sigma). Viral titer was determined by QuickTiter™ Lentivirus Associated HIV p24 Titer Kit (Cell Biolabs ...
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bioRxiv - Molecular Biology 2021Quote: ... All lentivectors and VLPs were DNAse treated (2 h at 37°C, DNaseI, Sigma) before they were concentrated by ultracentrifugation in a Sorvall Discovery (Hitachi ...
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bioRxiv - Microbiology 2023Quote: ... We collected VLP bands and buffer exchanged using a 100-kDa Amicon filter (Sigma). VLPs were buffer exchanged into DPBS (Thermo Fisher Scientific Cat # ...
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bioRxiv - Microbiology 2022Quote: ... varicella-zoster virus (Light Diagnostics, Varicella-Zoster Virus DFA kit, EMD Millipore, Temecula, CA), adenovirus (D3Ultra Respiratory Virus Screening & Identification kit ...
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bioRxiv - Microbiology 2021Quote: ... VLP-containing supernatant was filtered through a 0.2 um sterile syringe filter (Millex-GP, Millipore Sigma, USA) to remove the remaining bacteria ...
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bioRxiv - Microbiology 2022Quote: ... The VLP pellet was suspended in 1 mL SM buffer with lysozyme (10.0 mg/reaction; Sigma Aldrich) and incubated for 60 min at 37 °C with gentle shaking to degrade unfiltered bacterial cells ...
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bioRxiv - Microbiology 2024Quote: ... and VLPs were concentrated 10 times by the addition of PBS/8 % (w/v) PEG 6000 (Sigma), incubation at 4° C for 24 h ...
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bioRxiv - Cell Biology 2019Quote: ... Doxycycline-inducible OKSM virus (Millipore, SCR512) and m2rtTA virus (Cyagen Biosciences ...
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bioRxiv - Evolutionary Biology 2022Quote: ... VLP pellets were lysed in 60 μl of NP-40 lysis buffer containing 1% NP-40 (Sigma-Aldrich) and 10 μg/ml each of aprotinin ...
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bioRxiv - Microbiology 2021Quote: ... The VLP-containing supernatant was filtered through a 0.2 μm sterile syringe filter (Millex-GP, Millipore Sigma, USA) concentrated by centrifugation (35,000g ...
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bioRxiv - Pathology 2022Quote: ... the virus particle-containing media was harvested and virus particles were purified using Polyethylene glycol 8000 (Sigma).
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bioRxiv - Immunology 2021Quote: ... Qβ(1668)-VLPs were then derivatized for 30min at RT using dibenzocyclooctyne NHS ester (DBCO cross-linker) (Sigma-Aldrich) in a 5-fold molar excess ...
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bioRxiv - Microbiology 2019Quote: ... Anti-influenza A virus NP antibodies (Millipore), Anti-TNK2 (A12 ...
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bioRxiv - Microbiology 2022Quote: ... Virus propagation was performed in DMEM (Sigma) supplemented with 2% fetal bovine serum (Gibco) ...
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bioRxiv - Microbiology 2022Quote: ... Virus propagation was performed in DMEM (Sigma) supplemented with 2% fetal bovine serum (Gibco) ...
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bioRxiv - Microbiology 2020Quote: ... VLP samples were separated by 10% or 14% SDS-PAGE later blotted to PVDF membrane (Immobilon®-FL, Merck Millipore) and blocked overnight with ODYSSEY® blocking buffer ...
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bioRxiv - Molecular Biology 2021Quote: Retroviral infection was conducted in 6-well plates using either 1 mL of unconcentrated virus or 200 μL of concentrated virus in 20 mM HEPES pH 7.4 and 8 μg/mL polybrene (Sigma-Aldrich). If selection was desired ...
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bioRxiv - Cancer Biology 2020Quote: ... Virus infection was carried out by incubating cells with cell medium containing indicated virus and 5 μg/ml polybrene (Sigma) for 8-12 hours ...
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bioRxiv - Neuroscience 2020Quote: Virus was produced by polyethylenimine (PEI; Sigma-Aldrich) transfection of 89-90% confluent HEK293T cells with the viral vector together with the lentiviral packaging plasmids ...
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An endothelial SOX18-mevalonate pathway axis enables repurposing of statins for infantile hemangiomabioRxiv - Molecular Biology 2024Quote: ... or an empty vector control virus (Sigma SHC001V) followed by puromycin (1 μg/mL ...
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bioRxiv - Immunology 2020Quote: ... all CIS43 VLPs and wild-type (WT) Qβ VLPs were depleted of endotoxin by three rounds of phase separation using Triton X-114 (Sigma-Aldrich), as described in reference 48 ...
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bioRxiv - Immunology 2022Quote: ... or MDM (106) were treated for 1h at 37°C with 1 ml of VLPs-VPX+ containing 5 μg/ml polybrene (Sigma, #H9268) for SAMHD1 degradation16,37 before the addition of 20 µg CAp24 of each control or/and encoding-gene lentiviral vectors with 5 μg/ml polybrene for 2h transduction ...
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bioRxiv - Synthetic Biology 2019Quote: ... plates were coated with indicated concentrations of VLP (4°C, over-night) and subsequently blocked with 1% casein in PBS (Sigma-Aldrich) for 2h at 37°C before primary antibodies were added (polyclonal mouse anti-Pfs230 or monoclonal 1B3 antibody ...
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bioRxiv - Biophysics 2019Quote: ... Purified PCV2 VLPs were concentrated and buffer exchanged to phosphate buffered saline (PBS) using Amicon Utra-4 centrifugal filter devices (Merck Millipore, MA). PCV2 VLP samples were stored in 50-100 µl aliquots at −80°C.
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bioRxiv - Molecular Biology 2023Quote: ... ChAC-VLPs was further concentrated to 5 times of its initial volume using 100 kDa cut-off concentrator (Merck, Millipore, USA) followed by analysis on 10% Tris-Glycine SDS-PAGE and successful RBD-E2 fusion protein is validated by western blot using anti-RBD antibody.
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bioRxiv - Microbiology 2023Quote: ... VLPs were lysed by the addition of 100 μl of Phage Lysis Buffer (4.5 M guanidinium isothiocyanate (Sigma-Aldrich Cat#50983), 44 mM sodium citrate pH 7.0 (Sigma-Aldrich Cat#C8532) ...
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bioRxiv - Microbiology 2023Quote: ... The VLPs were subsequently concentrated down to 180 µL using Amicon Ultra-15 100K Centrifugal Filter Units (Milipore Sigma, MO, U.S.A). To remove any free contaminating nucleic acids ...
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bioRxiv - Cancer Biology 2021Quote: ... To transduce cells with virus cells were treated with both virus (MOI 0.5-1.0) and 5 μg / mL Polybrene infection reaction (EMD Millipore, TR-1003). For pLKO.1 vectors ...
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bioRxiv - Molecular Biology 2022Quote: ... For each 10 cm dish, 1 ml virus inoculum was prepared in virus growth media (VGM; containing DMEM, 0.2% bovine serum albumin (Sigma; Cat no. #A8412), 25 mM N-(2-hydroxyethyl ...
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bioRxiv - Microbiology 2020Quote: ... Aggregated virus was removed by a 0.22µm filter (Millipore). Sub-confluent monolayer of MEFs cultured in DMEM ...
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bioRxiv - Physiology 2020Quote: ... Virus was filtered with a 0.45mm OVDF filter (Millipore). Supernatants were collected and purified using PEG-it (System Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... supernatant containing virus was filtered with 0.22μm filters (Millipore) and store at −80◻ in aliquots.
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bioRxiv - Cancer Biology 2023Quote: ... adherent cells were infected with COL7A1 virus (Sigma Aldrich) (1–5 MOI (multiplicity of infection) ...
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bioRxiv - Molecular Biology 2023Quote: ... and the virus was concentrated using polyethylglycol (Sigma, P4338). Cells were infected in medium containing 5 μg ml−1 polybrene (Millipore ...
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bioRxiv - Biophysics 2020Quote: ... The supernatant was filtered using 0.2um filter and VLPs were captured in a 40-10% sucrose step gradient and concentrated using AMICON spin filters (UFC901008, EMD Millipore, Burlington, MA). Total protein yield for purified VLP stock varied somewhat but was typically above 5 mg/mL ...
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bioRxiv - Microbiology 2021Quote: ... Virus stocks were stored in aliquots containing 10% sucrose (Sigma) at −150°C ...
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Potential for virus endogenization in humans through testicular germ cell infection: the case of HIVbioRxiv - Microbiology 2020Quote: ... Input virus was treated with benzonase (500U/mL, Sigma-Aldrich) before cell exposure to reduce nucleic acid contamination in the viral stock ...
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bioRxiv - Neuroscience 2021Quote: ... Concentration of virus was repeated on smaller Amicon Ultracels (Millipore) until desired high titer was reached ...
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bioRxiv - Cell Biology 2021Quote: ... with 60 μg of Tobacco etch virus protease (Sigma-Aldrich) added in the dialysis cassette to cleave the histidine tag ...
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bioRxiv - Microbiology 2020Quote: ... virus was preincubated with the compound (human (Sigma-Aldrich #L8402) or chicken (Sigma-Aldrich #L4919 ...
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bioRxiv - Cell Biology 2022Quote: ... 100 μl of virus suspension and Polybrene (hexadimethrine bromide, Sigma) at a final concentration of 8 μg/ml were added ...
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bioRxiv - Microbiology 2023Quote: ... virus was filtered using a millipore filter (Millipore Sigma, SE1M179M6). On day of infection ...
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bioRxiv - Microbiology 2023Quote: ... virus was treated with alpha-1-antitrypsin (Sigma-Aldrich, SRP6312) at the concentration noted ...
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bioRxiv - Plant Biology 2023Quote: ... and then the tobacco etch virus (TEV) protease (Sigma-Aldrich) was added to remove the purification tags ...