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Citations for Millipore Sigma :
1 - 50 of 10000+ citations for Human Immunodeficiency Virus Gag Protein HIV 1 Clade B IIIB since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: HEK293T cells expressing HIV-1 Gag/Gag(i)mEos2 cultured on poly-l-lysine (Sigma) coated 25 mm round ♯ 1.5 coverslips (VWR ...
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bioRxiv - Microbiology 2021Quote: ... and 0.8 µg plasmid expressing HIV-1 gag-pol using X-tremeGENE 9 (Sigma). Media was changed 6 h later and lentivirus containing culture supernatants were collected at 48 and 72 h post-transfection ...
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bioRxiv - Microbiology 2019Quote: ... 1.8 μg of pRSV-Rev and 1.5 μg of plasmid containing pAIP-NSP2-mCherry or pAIP-NSP5 and the human immunodeficiency virus long terminal repeats were co-transfected with Lipofectamine 3000 (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2019Quote: ... For lipid mobility measurements inside and outside of Gag assembly sites in mammalian cells 3×105 Jurkat T cells 72 h post-infection with NL4.3 HIV-1 Gag-iGFP virus particles or 24 h post-transfection with NL4.3 HIV-1 Gag-eGFP expressing plasmid were resuspended in 200 µL of Leibovitz’s L 15 medium (Sigma) and incubated with 25 nM of the indicated fluorescent lipid analogue probe for 5 min at 37 °C ...
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bioRxiv - Immunology 2020Quote: ... MaxiSorp plates were coated with 1 μg/mL HIV-GP120 protein (Sigma-Aldrich). Samples were diluted in an eight-point two-fold dilution series ...
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bioRxiv - Microbiology 2019Quote: ... HIV-1 p24 (mouse, Millipore) and HIV-1 Nef (sheep ...
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bioRxiv - Microbiology 2024Quote: All transductions were performed via spin inoculation at 1050 x g for 2 hr at 25°C with equal virus amounts determined by Gag p24 mass in medium containing 4µg/mL polybrene (Sigma). MDMs were inoculated with 10µg p24 mass equivalents of NL4-3 ΔGPE-GFP virus or 20µg p24 mass equivalents of 3xFLAG-89.6-Vpr ...
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bioRxiv - Biochemistry 2020Quote: ... The Gag-His6 protein was loaded onto a HIS-select affinity column (Sigma), washed (20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... human liver cathepsin B (Millipore Sigma), and human cathepsin K (Enzo Sciences ...
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bioRxiv - Biochemistry 2024Quote: ... human Glu-Fibrinopeptide B (Sigma-Aldrich) was recorded throughout the analysis for lock-mass calibration ...
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bioRxiv - Molecular Biology 2023Quote: ... Buffer IIIb (3% Triton X-100, 1x T4 ligase buffer, 1x protease inhibitors; NEB, Sigma-Aldrich) was added to a final volume of 1 ml and sample was incubated for 15 min at 37 °C ...
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bioRxiv - Microbiology 2020Quote: ... virus was preincubated with the compound (human (Sigma-Aldrich #L8402) or chicken (Sigma-Aldrich #L4919 ...
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bioRxiv - Bioengineering 2019Quote: ... Human B-lymphoid (Sigma-Aldrich, Buchs Switzerland), 293T Flp-in T-REX (Life Technologies ...
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bioRxiv - Plant Biology 2020Quote: ... The human [Glu1]-fibrinopeptide B (Sigma-Aldrich) at 100 fmol/μl was used as an external calibrant and lock mass acquisition was performed every 30 s ...
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bioRxiv - Bioengineering 2023Quote: ... sulfated glycosaminoglycan (GAG) (Sigma Aldrich) and agarose (Sigma Aldrich ...
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bioRxiv - Molecular Biology 2020Quote: ... Gag was detected using mouse α-p24 (Millipore, diluted 1:5000), and GAPDH was detected with mouse α-GAPDH (GeneTex ...
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bioRxiv - Microbiology 2020Quote: ... HIV-1 PR cloning into the pET23a expression vector (Novagen), expression in Escherichia coli strain BL21 Star DE3 PlysS (Invitrogen) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... human recombinant MAO-A (hMAO-A) and MAO-B (hMAO-B) enzymes (Sigma-Aldrich) were diluted in 50 mM phosphate buffer (final protein amount ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-microtubule-associated protein 2a+b (MAP2, Sigma #M1406; 1:2000), rabbit anti-tyrosine hydroxylase (TH ...
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bioRxiv - Biochemistry 2022Quote: ... Standard curves were generated using recombinant HIV-1 RT (Merck Millipore). The relative viral quantities were calculated based on the standard curve generated using QuantStudio-7 systems software (Applied Biosystems).
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bioRxiv - Bioengineering 2020Quote: ... To the solution was added pre-warmed human cathepsin B (20 ng µL−1, EMD Millipore) in 30 µL MES buffer ...
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bioRxiv - Immunology 2024Quote: ... which after solidification were overlayed by basal medium containing Advanced DMEM/F12 + 1:100 Glutamax + 10 mM HEPES + 1.25 mM N-AcetylCystein + 1:50 B-27 Supplement + 1:100 N-2 Supplement + 50 ng/ml human EGF + 1:500 Primocin + 0.002% Heparin (Sigma), supplemented with additional factors as described in the main text ...
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bioRxiv - Microbiology 2023Quote: ... These proteins were cloned into pCDF1-b (Novagen) under the control of a T7 promoter ...
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bioRxiv - Biochemistry 2021Quote: ... and GAGs were incubated with streptavidin agarose (Sigma). Unbound GAGs were separated from GAGs captured on the streptavidin beads ...
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bioRxiv - Biochemistry 2021Quote: ... GAGs were reacted with sulfo-DBCO-biotin (Sigma) (6.7 mM ...
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bioRxiv - Microbiology 2022Quote: ... Plates with Towne virus were stained with mouse anti-human CMV IE1 (MAB810, Millipore) then goat anti-mouse IgG-AF488 (Millipore) ...
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bioRxiv - Cell Biology 2023Quote: ... 1% Amphotericin B (Sigma) and 1% HEPES (Sigma ...
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bioRxiv - Immunology 2020Quote: ... one 10-cm dish of packaging cell line at roughly 70% density was transfected with the target construct and two supplementing vectors providing the VSV gag/pol and VSV-G-env proteins (6 μg of DNA each) using polyethylenimine (22.5 μg/ml, Sigma) and Polybrene (4 μg/ml ...
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bioRxiv - Cell Biology 2019Quote: ... HIV-1+ or mitoTracker+ and CellTracker+ cells were then detached using accutase (Sigma) and co-cultured at a 1:1 ratio on glass coverslips in 24-well.
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bioRxiv - Biochemistry 2021Quote: ... Protein was eluted in buffer B with 0.5 M KCl and 1 mM methotrexate (Sigma). Then the protein was precipitated with 40% TCA for 60 minutes on ice followed by centrifugation (10000 g ...
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bioRxiv - Microbiology 2021Quote: ... The trypsin-released GAGs were treated with 1 mg/mL Pronase (Streptomyces griseus, Sigma Aldrich) and 0.1% Triton X-100 and 10 mM CaCl2 ...
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bioRxiv - Cell Biology 2019Quote: ... Proteins were probed with rabbit anti-human Cx43 (1:2000) (Sigma-Aldrich) and rabbit anti-ß-Tubulin antibodies (1:2000 ...
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bioRxiv - Cell Biology 2023Quote: ... HIV-Tat protein transduction motif (YGRKKRRQRRR) was subcloned into a pET-46 Ek/LIC vector (EMD Millipore #71335). The construct encoding a GST-tagged caltubin was subcloned into a pGEX-4T-1 vector (Amersham #28954549) ...
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bioRxiv - Microbiology 2024Quote: ... HA tagged proteins were detected in virus lysates using rabbit polyclonal anti-HA antibody (Cat# H6908, Sigma, 1:1,000). The V5 tagged proteins were detected in cell and virus lysates using anti-V5 mouse monoclonal antibody (Cat# V8012 ...
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bioRxiv - Cell Biology 2019Quote: ... GAG was quantified using the 1,9-dimethymethylene blue (Sigma) assay ...
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bioRxiv - Cancer Biology 2023Quote: ... The membranes were incubated in a primary rabbit α-human A3A/B/G antibody 1:1,000 (Brown et al.) and mouse α-tubulin 1:10,000 (Sigma Aldrich), or rabbit α-actin 1:5,000 (Sigma Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... HIV-1 Env-pseudotyped lentivirus in growth medium supplemented with DEAE-dextran (Millipore Sigma) was added to the serially diluted serum samples.Medium was removed from cells and replaced with 100 μL medium containing the diluted mouse serum and virus ...
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bioRxiv - Systems Biology 2021Quote: ... virus protein-bound host proteins were enriched by adding 50 µl anti-HA-agarose slurry (Sigma-Aldrich, A2095) with constant agitation for 3 hours at 4°C ...
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bioRxiv - Cell Biology 2021Quote: ... antifoam B (Sigma, 1:5000), and 40U/mL RNAsin (Promega)) ...
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bioRxiv - Cell Biology 2020Quote: ... 1:5000 Antifoam B (Sigma) and lysed by vortexing with acid-washed glass beads for 2 min followed by 2 min on ice for three cycles ...
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Epigenetic deregulation of IFN and WNT pathways in AT2 cells impairs alveolar regeneration (in COPD)bioRxiv - Cell Biology 2023Quote: ... and 1% amphotericin B (Sigma) and processed as previously published (Llamazares-Prada et al ...
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bioRxiv - Microbiology 2020Quote: ... Cells were infected with HIV-1NL4-3 or HIV-1JRFL (PM1-CCR5) and cultured in RPMI 1640 medium (Sigma-Aldrich) supplemented with 10% fetal calf serum (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... mouse anti-α-tubulin (B-5-1-2) (1:10000; Sigma-Aldrich T5168, B-5-1-2), mouse anti-GAPDH (1:25000 ...
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bioRxiv - Molecular Biology 2020Quote: ... and expression of the protein was induced for 4 hours with 1 mM isopropyl 1-thio-B-D-galactopyranoside (Sigma) at 18°C ...
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bioRxiv - Microbiology 2020Quote: ... except that HIV-1 reverse transcriptase (RTase) was used in the extension assay (Millipore-Sigma). Further details are described in the S1 Supplemental Methods.
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bioRxiv - Microbiology 2020Quote: ... except that HIV-1 reverse transcriptase (RTase) was used in the extension assay (Millipore-Sigma). Further details are described in the S1 Supplemental Methods.
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bioRxiv - Microbiology 2022Quote: ... except that HIV-1 reverse transcriptase (RTase) was used in the extension assay (Millipore-Sigma). The extension reactions were performed at 37 °C for 40 min and included ∼6 to 1200 fmol of total dNTP ...
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bioRxiv - Microbiology 2022Quote: ... except that HIV-1 reverse transcriptase (RTase) was used in the extension assay (Millipore-Sigma). The extension reactions were performed at 37 °C for 40 min and included ∼6 to 1200 fmol of total dNTP ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... HIV Reagent Program) followed by an actin mouse monocolonal antibody (1:5000 dilution for 1 h; Millipore #MAB1501R). Nef and actin proteins were visualized with complementary anti-rabbit and anti-mouse secondary antibodies coupled to infrared dyes for quantitative imaging using the LI-COR platform.
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bioRxiv - Immunology 2021Quote: ... were coated with 50 µL/well of 1 ng/µL solution of fragments 308-331 or 421-438 from HIV gp120 peptides or fragment 579-601 of HIV gp41 (Sigma-Aldrich) in carbonate-bicarbonate buffer (pH 9.6 ...