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Citations for Millipore Sigma :
1 - 50 of 10000+ citations for Human Carboxypeptidase M CPM Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: 100% x (experimental cpm- background cpm) (total cpm-background cpm) Total release of 51Cr was assessed by lysis with 10% SDS (Sigma, St Louis, Mo) and background release was measured in the absence of effector cells and antibodies.
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The activator domain of bacterial collagenases drives collagen recognition, unwinding and processingbioRxiv - Biochemistry 2023Quote: ... CPM (Sigma-Aldrich, Germany) was dissolved at 4 mg/ml in DMSO and stored at -80 °C ...
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bioRxiv - Biochemistry 2021Quote: ... The mixture was treated with carboxypeptidase A (Sigma) overnight at 4 °C to remove the His-tags ...
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bioRxiv - Synthetic Biology 2020Quote: ... The RBD was treated with endoglycosidase H42 and Carboxypeptidase A (Sigma-Aldrich), to remove the N-glycans and the C-terminal 6xHis tag ...
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bioRxiv - Developmental Biology 2020Quote: ... 0.126 M/mL human insulin (Sigma), and 100 nM dexamethasone ...
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bioRxiv - Microbiology 2020Quote: ... This was removed by first incubating β-IgSF by itself with Carboxypeptidase A (Sigma). 2 mL of 6 mg mL−1 of β-IgSF was incubated with 1:100 w/w of Carboxypeptidase A for 24 hours at 4 °C before the addition of another 1:100 w/w of Carboxypeptidase A and incubation for 24 hours at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... the His-tag was removed by treating purified nanobody Nb_0 with carboxypeptidase A (Sigma) and B (Roche ...
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bioRxiv - Developmental Biology 2019Quote: ... and the histidine tag removed with carboxypeptidase A bound to agarose (Sigma, St. Louis, MO) at a ratio of 10 protein:1 enzyme (w/w) ...
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bioRxiv - Neuroscience 2020Quote: ... CPM: N2B27 supplemented with 100 nM Retinoic Acid (RA; Millipore Sigma), 200 nM Hh-Ag1.5 ...
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bioRxiv - Biochemistry 2023Quote: ... the solubilized tubulin in SN3 was incubated with 1/300 volume of Carboxypeptidase A (Sigma C9268) at 30°C for 5 min before the incubation in high molarity PIPES buffer ...
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bioRxiv - Cell Biology 2023Quote: N-[4-(7-diethylamino-4-methyl-3-coumarinyl)phenyl]maleimide (CPM, Sigma) was dissolved in DMSO at 4 mg/ml as stock solution and diluted 20 times using a buffer containing 25 mM HEPES ...
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bioRxiv - Cancer Biology 2023Quote: ... The M-protein solutions were diluted 1:1 with 8 M Urea (Sigma Aldrich) in Tris.HCl (pH=7 ...
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bioRxiv - Neuroscience 2023Quote: ... human iPSC-derived neurons were pretreated with 10 M bumetanide (Sigma) or the corresponding vehicle (0.01% DMSO ...
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bioRxiv - Neuroscience 2022Quote: ... Pelleted proteins were resuspended in 0.1 M NaOH (Sigma) and assayed for protein quantification using the Bradford assay (Biorad).
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bioRxiv - Biophysics 2022Quote: ... A negative control without tyrosinated tubulin was prepared by treating 3 mg/mL bovine tubulin with 0.02 mg/mL carboxypeptidase A (CPA, Sigma Aldrich) on ice for 15 min to proteolytically cleaved the C-terminal tyrosine of α-tubulin ...
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bioRxiv - Immunology 2022Quote: ... Macrophage colony-stimulating factor (M-CSF, human) was purchased from Millipore Sigma, St ...
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bioRxiv - Physiology 2019Quote: ... Proteins were resuspended in concentrated urea buffer (8 M Urea, 2 M Thiourea, 150 mM NaCl (Sigma)) ...
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bioRxiv - Cell Biology 2022Quote: Proteins were reduced with 2.5 μl 0.2 M dithiothreitol (Sigma) for one hour at 57 °C at pH 7.5 ...
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bioRxiv - Biophysics 2021Quote: Fibrinogen (human plasma purified protein, Sigma-Aldrich)
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bioRxiv - Evolutionary Biology 2019Quote: ... Bound dARC1 CA was eluted in non-reducing buffer (50 mM Tris-HCl, 150 mM NaCl, 300 mM Imidazole) and Carboxypeptidase A (CPA, Sigma C9268) was added at a ratio of ∼ 100 mg dARC1 per mg CPA ...
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bioRxiv - Microbiology 2023Quote: ... Enriched proteins were denatured in U/T buffer (6 M urea, 2 M thiourea, 1 mM DTT (Sigma), 10 mM HEPES ...
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bioRxiv - Microbiology 2021Quote: ... Primary human monocytes were treated with 50 ng/ml M-CSF (Sigma-Aldrich) in complete RPMI for a week before infection ...
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bioRxiv - Molecular Biology 2022Quote: ... 3×10-4 M monothioglycerol and 300 μg/ml human transferrin (Sigma Aldrich). Cells were plated in petri dishes (Thermo Fisher ...
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bioRxiv - Microbiology 2021Quote: ... Putative ligand proteins (elastin from human skin, fibrinogen from human plasma, laminin from human placenta, fibronectin from human plasma) (all from Sigma) were resuspended in carbonate-bicarbonate buffer (Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... and protein concentration was measured with BCA assay (Sigma 71285-M). Then ...
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bioRxiv - Microbiology 2023Quote: ... and 0.5 M NaCl) before eluting bound proteins with buffer A containing 0.5 M Methyl-α-D mannose (Sigma). Fractions containing NA activity were pooled ...
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bioRxiv - Microbiology 2024Quote: ... The protein pellet was washed in protein wash buffer (0.3 M guanidine hydrochloride (Sigma Aldrich, USA) in 95% ethanol ...
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bioRxiv - Biophysics 2021Quote: ... Plasminogen (human plasma purified protein, Sigma-Aldrich, Germany) and Fibrinogen (human plasma purified protein ...
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bioRxiv - Biophysics 2021Quote: ... and Fibrinogen (human plasma purified protein, Sigma-Aldrich). Experimental conditions are presented in the table I.
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bioRxiv - Biophysics 2021Quote: Plasminohen (human plasma purified protein, Sigma-Aldric, Germany)
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bioRxiv - Developmental Biology 2019Quote: ... 200nL 0.1µg/µL human noggin protein (Sigma H6416) was injected into the blastocoel of embryos at stage 8 ...
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bioRxiv - Neuroscience 2019Quote: ... and His-tagged human HDAC6 protein (EMD Millipore) were incubated with 57 nm 32P-ATP in kinase buffer (50 mM Tris HCl ...
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bioRxiv - Microbiology 2020Quote: ... 5 μg human insulin mL-1 and 5×10-7 M hydrocortisone hemisuccinate (Sigma). Cells were seeded and expanded for two weeks and subsequently differentiated for another two weeks in the presence of 1.8% DMSO61 (dHepaRG).
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bioRxiv - Microbiology 2020Quote: ... 5 μg human insulin mL−1 and 5×10−7 M hydrocortisone hemisuccinate (Sigma). As a control we generated HepaRG cells expressing an inactive HBx null mutant (HepaRG-HBxSTOP ...
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bioRxiv - Cancer Biology 2023Quote: ... The eluted M-protein fractions were collected in Eppendorf tubes prefilled with 5 μl 2 M TRIS.HCl (pH=7, Sigma Aldrich), to neutralize the solution ...
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bioRxiv - Biophysics 2020Quote: Proteins were buffer exchanged into 1 M aqueous ammonium acetate (Sigma-Aldrich) using P6 Biospin columns (Bio-Rad ...
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bioRxiv - Cell Biology 2023Quote: ... Whole-cell protein lysates were prepared in CelLytic M lysis buffer (Sigma) with 1% protease/phosphatase inhibitors (Sigma ...
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bioRxiv - Bioengineering 2020Quote: ... or human hemoglobin (all proteins were purchased from Sigma). Protein nanorods were prepared using chemically modified human serum albumin.
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bioRxiv - Developmental Biology 2021Quote: ... Total proteins were extracted from cells with the M-PER Mammalian Protein Extraction Reagent (Sigma, GE28-9412-79) supplemented with protease and phosphatase inhibitors (Roche) ...
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bioRxiv - Microbiology 2022Quote: Coding sequences for M proteins were cloned into a modified pET28b vector (Novagen) that contained sequences encoding an N-terminal His6-tag followed by a PreScission protease cleavage site (5) ...
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bioRxiv - Neuroscience 2022Quote: ... Protein was extracted using 100 µL of lysis buffer (7 M urea (Sigma), 2 M thiourea (Sigma ...
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bioRxiv - Cell Biology 2021Quote: Protein was extracted from cells using lysis buffer (CelLytic™ M, Sigma-Aldrich) supplemented with Halt™ protease/phosphatase inhibitor cocktail (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2023Quote: ... protein was extracted from SC cells using lysis buffer (CelLyticTM M, Sigma-Aldrich) supplemented with Halt™ protease/phosphatase inhibitor cocktail (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2024Quote: ... proteins were solubilised in solubilising buffer containing 1 M hydroxylamine hydrochloride (159417, Sigma), 1 mM HPDP-biotin (A8008 ...
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bioRxiv - Cancer Biology 2020Quote: ... and purified human GST protein (5 mg/ml) (Sigma-Aldrich) were incubated for 30min with biotinylated probes (100 pM ...
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bioRxiv - Biophysics 2023Quote: Oxotremorine-M (OXO-M) (Sigma-Aldrich) was dissolved in distilled water to make a 50 mM stock solution and further diluted in the bath solution for the final concentration 50 µM ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: We extracted the soluble protein from the damselflies using 0.1 M NaOH (Sigma-Aldrich) as a lysis buffer ...
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bioRxiv - Cell Biology 2020Quote: ... Protein pellets were solubilised in 30 μl of 2 M Tris pH 8.0 (Sigma) / 70μl 3X SDS-PAGE loading buffer (60 mM Tris pH 6.8 (Sigma) ...
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bioRxiv - Bioengineering 2023Quote: ... then resuspended in 100 µL protein storage buffer: 4 M urea (Sigma-Aldrich, U5128), 1 % SDS (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... Elution step of enriched proteins was performed using 2.25 M ammonia solution (Sigma, #5.33003) for 16 h ...