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Citations for Millipore Sigma :
1 - 50 of 9960 citations for GM CSF Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... GM-CSF (Sigma-Aldrich, St. Louis, MO). Fibroblasts were monitored for YFP ...
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bioRxiv - Immunology 2023Quote: ... and GM-CSF were quantified by multiplex (Millipore) according to manufacturers’ protocols.
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 20 ng/mL mouse GM-CSF (Miltenyi #130-095-739) and 0.5 μM β-estradiol (Sigma-Aldrich #E2758)] and transduced with 1:5 (vol:vol ...
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bioRxiv - Cell Biology 2020Quote: ... always enriched with granulocyte-macrophage colony stimulating factor (5ng/ml GM-CSF, Sigma). After confluence (at 37°C ...
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bioRxiv - Immunology 2020Quote: ... recombinant GM-CSF (10ng/ml; Peprotec) and β-estradiol (1 μM; Sigma-Aldrich). To differentiate into macrophages ...
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bioRxiv - Neuroscience 2021Quote: ... enriched with the granulocyte-monocyte colony stimulating factor (5ng/mL GM-CSF, Sigma) to promote microglia proliferation at 37°C ...
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bioRxiv - Microbiology 2020Quote: ... and 25 ng/mL of human granulocyte-macrophage colony-stimulating factor (GM-CSF, Sigma-Aldrich) for five days ...
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bioRxiv - Immunology 2023Quote: ... 10 ng/mL GM-CSF and 10ug/mL SOAT1i Sandoz 58-035 (Sigma Cat# S9318-5MG) were added to the media during equilibration to assess their impact on efflux rates
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bioRxiv - Cancer Biology 2021Quote: ... and GM-CSF levels were quantified using ELISA kits pre-coated with indicated capture antibodies per manufacturer’s instructions (Sigma). IL-6 levels were preliminarily detected using a Q-Plex Human cytokine screen (16-plex ...
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bioRxiv - Cell Biology 2021Quote: ... Rose-John), 20 U/ml recombinant murine GM-CSF (PharmedArtis, Germany) and 10−6 M dexamethasone (Sigma-Aldrich, USA). Progenitor cells were amplified 5 to 11 days before differentiation into DC was induced by supplementing 200 U/ml recombinant murine GM-CSF ...
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bioRxiv - Immunology 2022Quote: ... bacteria were diluted further in the same medium supplemented with 0.5 ng/mL granulocyte macrophage colony stimulating factor (GM-CSF, Millipore Sigma), to the required multiplicity of infection (MOI).
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bioRxiv - Immunology 2020Quote: ... After transduction cells were cultured in with media supplemented with supernatant from B16 cells expressing GM-CSF and 10uM estradiol (Sigma E8875) to generate macrophage progenitor cell lines or in media supplemented with supernatant from B16 cells expressing FLT3L and 10uM estradiol (Sigma E8875 ...
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bioRxiv - Microbiology 2022Quote: ... CD14+ monocytes were seeded at 106 cells/ml and cultured in R10 media (RPMI supplemented with FBS, HEPES, L-glutamine, and pen/strep) with 50 ng/ml of GM-CSF (Sigma Aldrich G5035) in a 37°C incubator ...
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bioRxiv - Microbiology 2023Quote: ... anti-His (mouse, 1:3k, Sigma, A7058), anti-c-Myc (mouse ...
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bioRxiv - Immunology 2020Quote: ... GM-CSF and TNF-α using the Luminex kit according to the manufacturer’s instructions (Milliplex Multiplex Assays with Luminex, Millipore Sigma, Danvers, MA). Samples were assayed using the Luminex assay system (Magpix).
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bioRxiv - Biochemistry 2019Quote: ... Culture medium for AML193 cells was supplemented with 0.005 mg/ml insulin and 5 ng/ml GM-CSF (Sigma-Aldrich, St. Louis, MO). Cells were cultured at 37°C in a humidified 5% CO2 atmosphere ...
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bioRxiv - Microbiology 2020Quote: ... Mouse anti-His antibody (1:12000) (Sigma-Aldrich) and biotinylated PNA (1:6000 ...
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bioRxiv - Plant Biology 2021Quote: ... the liquid GM was replaced with liquid GM containing 50 μM ABA (Sigma) or 0.1% EtOH as a solvent control ...
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bioRxiv - Cell Biology 2022Quote: ... mouse α-His-HRP (A7058, Sigma-Aldrich, 1:10000).
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bioRxiv - Microbiology 2021Quote: ... Antibodies used for protein quantification for ELISAs were mouse monoclonal anti-His (His-Tag mAb, EMD-Millipore), and biotinylated mouse anti-rat Cd4 (clone OX68) ...
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bioRxiv - Cell Biology 2019Quote: C2C12 mouse myoblasts were cultured in Growth medium (GM): Dulbecco’s modified Eagle’s medium (DMEM, Sigma) supplemented with 15% Fetal Calf Serum (FCS ...
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bioRxiv - Cell Biology 2019Quote: ... 10 μM GM-6001 (Millipore) in 10 μL DMSO ...
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bioRxiv - Microbiology 2022Quote: ... Proteins were detected using α -His from mouse (Sigma H1029), α-Gfp from mouse ...
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bioRxiv - Neuroscience 2022Quote: ... mouse anti-His antibody (Sigma; 1:5,000 in PBS + 2% BSA) was added and incubated for 1h ...
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bioRxiv - Plant Biology 2021Quote: ... for cycloheximide treatments the liquid GM was replaced with liquid GM containing 0.2 mg/mL cycloheximide (Sigma) or plain liquid GM as a mock control ...
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bioRxiv - Biochemistry 2021Quote: HEK293 cells (Sigma:85120602) were cultured using DMEM (Gibco™ ...
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bioRxiv - Biophysics 2023Quote: HEK293 cells (Sigma Aldrich) and were cultured in DMEM (Gibco ...
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bioRxiv - Microbiology 2021Quote: ... and extensively dialysed against HEPES-buffered saline and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag mAb, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (Bethyl).
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bioRxiv - Cell Biology 2022Quote: ... and extensively dialysed against HBS and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag monoclonal antibody, 70796, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (A3562 ...
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bioRxiv - Biophysics 2023Quote: ... then incubated with mouse anti-His tag primary antibody (Millipore Sigma SAB1305538) at 1:1,000 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... Mevalonic acid lactone (Sigma M4667, 1 gm) was converted to its active form by dissolving in ethanol (3.5 ml) ...
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bioRxiv - Molecular Biology 2019Quote: ... 2 gm/L D-Glucose (Sigma-Aldrich), 2 gm/L Sodium bicarbonate (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2019Quote: ... 2 gm/L Sodium bicarbonate (Sigma-Aldrich), and 56 mg/L of Gentamycin (Abbott) ...
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bioRxiv - Microbiology 2022Quote: ... with the following modifications: Mouse anti-GST tag or mouse anti-His tag 1:200× (Sigma-Aldrich) and HRP-conjugated goat anti-mouse IgG 1:2,000× (Seracare Life Sciences ...
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bioRxiv - Cell Biology 2024Quote: ... respectively (Millipore Cat# HI-14K, HI-13K). Samples treated with 3 mM glucose KRBH were measured undiluted ...
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bioRxiv - Biochemistry 2021Quote: HEK293 and HEK293T cells (Sigma) were cultured at 37 °C ...
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bioRxiv - Biochemistry 2022Quote: ... S5H recombinant protein was examined by using specific anti-His mouse antibody (Novagen) as described in (López-Gresa et al. ...
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bioRxiv - Biochemistry 2020Quote: ... The production of the (His)6-tagged recombinant proteins was confirmed by Western analysis using mouse monoclonal α-His-horseradish peroxidase conjugated antibodies (Sigma Aldrich, USA). Imaging of the protein bands on the Western blot was conducted using the ECL kit (ThermoScientific ...
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bioRxiv - Synthetic Biology 2021Quote: ... and 200 µg mL-1 gentamicin (Gm, Sigma). We used 1 mM of Isopropyl β-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Microbiology 2020Quote: ... by dissolving 1 gm of agarose (Sigma-Aldrich) into 200 mL of Tris-acetate-EDTA (TAE ...
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bioRxiv - Plant Biology 2022Quote: ... 2 gm of phloroglucinol powder (Sigma-Aldrich, USA) was dissolved in 80 ml of 20 % ethanol and 20 ml of concentrated HCl ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 gm/L sodium bicarbonate (Sigma-Aldrich, USA), and 10 mg/L gentamicin (Gibco ...
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bioRxiv - Molecular Biology 2022Quote: ... and 2 gm/L sodium bicarbonate (Sigma, USA). Culture was maintained at 37°C in 90% N2 ...
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bioRxiv - Cell Biology 2024Quote: ... 2 gm/L sodium bicarbonate (Sigma-Aldrich, USA), 5 gm/L AlbuMax I ...
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bioRxiv - Microbiology 2024Quote: ... and 15 μg mL-1 gentamycin (Gm, Sigma). L-arabinose (Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... bound proteins were detected by immunoblotting with primary mouse anti-His (Sigma□Aldrich, Taiwan) or anti-GST primary antibodies (Sigma□Aldrich ...
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bioRxiv - Biochemistry 2024Quote: ... and dilutions were as follows (in parenthesis): His (mouse, Sigma cat# H1029; 1:2,500); β-actin (rabbit ...
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bioRxiv - Microbiology 2022Quote: ... HEK293 WT (Sigma Cat. Nr. 85120602), HEK293 GALNT1 KO (Narimatsu et al. ...
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bioRxiv - Immunology 2021Quote: ... HEK293 cells (Sigma Aldrich 12022001-1VL) were maintained in DMEM supplemented with L-glutamine and 10% FBS ...
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bioRxiv - Immunology 2022Quote: ... Non-specific binding sites were blocked using fluorescence-activated cell sorting (FACS) buffer (1× PBS, 2% hi-FBS) supplemented with 20% hi-FBS and 10 μg mouse IgG (Sigma-Aldrich, St-Louis, MO, USA). Cells were stained using the following conjugated mouse anti-human monoclonal antibodies (mAbs) ...