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Citations for Millipore Sigma :
1 - 50 of 3562 citations for GM CSF Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... GM-CSF (Sigma-Aldrich, St. Louis, MO). Fibroblasts were monitored for YFP ...
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bioRxiv - Microbiology 2020Quote: ... and 25 ng/mL of human granulocyte-macrophage colony-stimulating factor (GM-CSF, Sigma-Aldrich) for five days ...
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bioRxiv - Immunology 2023Quote: ... and GM-CSF were quantified by multiplex (Millipore) according to manufacturers’ protocols.
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bioRxiv - Cell Biology 2020Quote: ... always enriched with granulocyte-macrophage colony stimulating factor (5ng/ml GM-CSF, Sigma). After confluence (at 37°C ...
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bioRxiv - Immunology 2020Quote: ... recombinant GM-CSF (10ng/ml; Peprotec) and β-estradiol (1 μM; Sigma-Aldrich). To differentiate into macrophages ...
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bioRxiv - Neuroscience 2021Quote: ... enriched with the granulocyte-monocyte colony stimulating factor (5ng/mL GM-CSF, Sigma) to promote microglia proliferation at 37°C ...
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bioRxiv - Biochemistry 2022Quote: Human embryonic kidney 293 cells (HEK293) (Sigma), HEK293T (TakaraBio) ...
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bioRxiv - Immunology 2023Quote: ... 10 ng/mL GM-CSF and 10ug/mL SOAT1i Sandoz 58-035 (Sigma Cat# S9318-5MG) were added to the media during equilibration to assess their impact on efflux rates
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bioRxiv - Cancer Biology 2021Quote: ... and GM-CSF levels were quantified using ELISA kits pre-coated with indicated capture antibodies per manufacturer’s instructions (Sigma). IL-6 levels were preliminarily detected using a Q-Plex Human cytokine screen (16-plex ...
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bioRxiv - Cell Biology 2021Quote: ... Rose-John), 20 U/ml recombinant murine GM-CSF (PharmedArtis, Germany) and 10−6 M dexamethasone (Sigma-Aldrich, USA). Progenitor cells were amplified 5 to 11 days before differentiation into DC was induced by supplementing 200 U/ml recombinant murine GM-CSF ...
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bioRxiv - Immunology 2022Quote: ... bacteria were diluted further in the same medium supplemented with 0.5 ng/mL granulocyte macrophage colony stimulating factor (GM-CSF, Millipore Sigma), to the required multiplicity of infection (MOI).
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bioRxiv - Cell Biology 2023Quote: ... supplemented with 20 ng/mL mouse GM-CSF (Miltenyi #130-095-739) and 0.5 μM β-estradiol (Sigma-Aldrich #E2758)] and transduced with 1:5 (vol:vol ...
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bioRxiv - Neuroscience 2019Quote: ... and His-tagged human HDAC6 protein (EMD Millipore) were incubated with 57 nm 32P-ATP in kinase buffer (50 mM Tris HCl ...
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bioRxiv - Cell Biology 2024Quote: HEK293 (Human Embryonic Kidney, ECACC 96121229 from Sigma-Aldrich Chemie GmbH) were cultured in DMEM (Dulbecco’s modified Eagle’s medium PAN biotech ...
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bioRxiv - Immunology 2020Quote: ... After transduction cells were cultured in with media supplemented with supernatant from B16 cells expressing GM-CSF and 10uM estradiol (Sigma E8875) to generate macrophage progenitor cell lines or in media supplemented with supernatant from B16 cells expressing FLT3L and 10uM estradiol (Sigma E8875 ...
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bioRxiv - Immunology 2022Quote: ... Macrophage colony-stimulating factor (M-CSF, human) was purchased from Millipore Sigma, St ...
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bioRxiv - Microbiology 2022Quote: ... CD14+ monocytes were seeded at 106 cells/ml and cultured in R10 media (RPMI supplemented with FBS, HEPES, L-glutamine, and pen/strep) with 50 ng/ml of GM-CSF (Sigma Aldrich G5035) in a 37°C incubator ...
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bioRxiv - Immunology 2020Quote: ... GM-CSF and TNF-α using the Luminex kit according to the manufacturer’s instructions (Milliplex Multiplex Assays with Luminex, Millipore Sigma, Danvers, MA). Samples were assayed using the Luminex assay system (Magpix).
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bioRxiv - Biochemistry 2019Quote: ... Culture medium for AML193 cells was supplemented with 0.005 mg/ml insulin and 5 ng/ml GM-CSF (Sigma-Aldrich, St. Louis, MO). Cells were cultured at 37°C in a humidified 5% CO2 atmosphere ...
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bioRxiv - Microbiology 2021Quote: ... Primary human monocytes were treated with 50 ng/ml M-CSF (Sigma-Aldrich) in complete RPMI for a week before infection ...
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bioRxiv - Microbiology 2022Quote: ... and HEK293-ACE2 cells [HEK293 cells (ATCC CRL-1573) stably expressing human ACE2]22 were maintained in DMEM (high glucose) (Sigma-Aldrich, Cat# 6429-500ML) containing 10% FBS ...
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bioRxiv - Neuroscience 2019Quote: ... was incubated with recombinant His-tagged human HDAC6 protein (EMD Millipore) in 250 μl RB100 buffer (25 mM HEPES pH 7.5 ...
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bioRxiv - Plant Biology 2021Quote: ... the liquid GM was replaced with liquid GM containing 50 μM ABA (Sigma) or 0.1% EtOH as a solvent control ...
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bioRxiv - Biochemistry 2022Quote: ... and another was loaded with 10 ng of human HSP70 (His tagged human HSP70, SRP5190, Millipore Sigma), which allowed comparisons to be made among separate gels ...
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bioRxiv - Cell Biology 2019Quote: ... 10 μM GM-6001 (Millipore) in 10 μL DMSO ...
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bioRxiv - Neuroscience 2020Quote: ... Cultured human embryonic kidney 293 (HEK293) cells were transfected using 2 μg/μl polyethylenimine (Sigma-Aldrich) with indicated plasmids for the expression of either Flag-KCTDs alone or in combination with Flag-Cav2.3 ...
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bioRxiv - Neuroscience 2019Quote: ... and PAK/cofilin mechanism experiments used human embryonic kidney cells (HEK293) cultured in DMEM:F12 (Sigma, D6421) supplemented with 10% foetal bovine serum (ClonTech ...
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bioRxiv - Biophysics 2021Quote: His-tagged human PCNA protein was purchased from Sigma Aldrich (catalogue number SRP5117), at a concentration of 6.6 μM ...
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bioRxiv - Plant Biology 2021Quote: ... for cycloheximide treatments the liquid GM was replaced with liquid GM containing 0.2 mg/mL cycloheximide (Sigma) or plain liquid GM as a mock control ...
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bioRxiv - Biochemistry 2021Quote: HEK293 cells (Sigma:85120602) were cultured using DMEM (Gibco™ ...
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bioRxiv - Biophysics 2023Quote: HEK293 cells (Sigma Aldrich) and were cultured in DMEM (Gibco ...
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bioRxiv - Neuroscience 2021Quote: Human embryonic kidney (HEK293) cells were maintained in a 37°C/5% CO2 atmosphere in DMEM:F12 (Sigma, D6421) supplemented with 10% foetal bovine serum (ClonTech ...
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bioRxiv - Cell Biology 2021Quote: ... Mevalonic acid lactone (Sigma M4667, 1 gm) was converted to its active form by dissolving in ethanol (3.5 ml) ...
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bioRxiv - Molecular Biology 2019Quote: ... 2 gm/L D-Glucose (Sigma-Aldrich), 2 gm/L Sodium bicarbonate (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2019Quote: ... 2 gm/L Sodium bicarbonate (Sigma-Aldrich), and 56 mg/L of Gentamycin (Abbott) ...
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bioRxiv - Cell Biology 2024Quote: ... respectively (Millipore Cat# HI-14K, HI-13K). Samples treated with 3 mM glucose KRBH were measured undiluted ...
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bioRxiv - Biochemistry 2021Quote: HEK293 and HEK293T cells (Sigma) were cultured at 37 °C ...
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bioRxiv - Microbiology 2020Quote: The human embryonic kidney cell lines HEK293 and HEK293T were cultured in Dulbecco’s modified Eagle’s medium (D1152, Sigma-Aldrich) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2023Quote: U2OS osteosarcoma cells and HEK293 (human embryonic kidney) cells were grown in DMEM (Dulbecco’s Modified Eagle’s Medium; Sigma, D1152) supplemented with 10% fetal bovine serum (FCS ...
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bioRxiv - Synthetic Biology 2021Quote: ... and 200 µg mL-1 gentamicin (Gm, Sigma). We used 1 mM of Isopropyl β-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Microbiology 2020Quote: ... by dissolving 1 gm of agarose (Sigma-Aldrich) into 200 mL of Tris-acetate-EDTA (TAE ...
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bioRxiv - Plant Biology 2022Quote: ... 2 gm of phloroglucinol powder (Sigma-Aldrich, USA) was dissolved in 80 ml of 20 % ethanol and 20 ml of concentrated HCl ...
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bioRxiv - Molecular Biology 2022Quote: ... 2 gm/L sodium bicarbonate (Sigma-Aldrich, USA), and 10 mg/L gentamicin (Gibco ...
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bioRxiv - Molecular Biology 2022Quote: ... and 2 gm/L sodium bicarbonate (Sigma, USA). Culture was maintained at 37°C in 90% N2 ...
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bioRxiv - Cell Biology 2024Quote: ... 2 gm/L sodium bicarbonate (Sigma-Aldrich, USA), 5 gm/L AlbuMax I ...
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bioRxiv - Microbiology 2024Quote: ... and 15 μg mL-1 gentamycin (Gm, Sigma). L-arabinose (Sigma ...
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bioRxiv - Immunology 2022Quote: ... Antibody production was conducted by co-transfection of the heavy and light chain expression vectors into Human HEK293 suspension culture cells (Expi293F) using polyethylene mine (Sigma). Cells were subsequently cultured for 4 days before being pelleted by centrifugation ...
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bioRxiv - Microbiology 2022Quote: ... HEK293 WT (Sigma Cat. Nr. 85120602), HEK293 GALNT1 KO (Narimatsu et al. ...
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bioRxiv - Immunology 2021Quote: ... HEK293 cells (Sigma Aldrich 12022001-1VL) were maintained in DMEM supplemented with L-glutamine and 10% FBS ...
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bioRxiv - Microbiology 2021Quote: ... and 1x non-essential amino acid) and 50 ng/mL of human macrophage colony-stimulating factor (M-CSF, Millipore, Temecula, CA) for 5-6 days at 37°C and 5% CO2 ...