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Citations for Millipore Sigma :
1 - 50 of 3036 citations for Ferritin heavy chain FTH1 Human since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... heavy Chain Specific (Sigma-Aldrich); AlexaFluor-488,-568 and -647 conjugated secondary antibodies and AlexaFluor -488 ...
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bioRxiv - Cell Biology 2023Quote: ... myosin heavy-chain (M4276, Millipore Sigma), myogenin (ab1835 ...
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bioRxiv - Bioengineering 2022Quote: ... anti-myosin heavy chain (Merck Millipore, A4.1025), anti-CD31 (Abcam ...
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bioRxiv - Immunology 2020Quote: ... monoclonal antibodies were produced by transient co-transfection of 293-F cells with human heavy chain and light chain antibody expression plasmids using polyethylenimine (PEI) (Sigma-Aldrich, catalog #408727). Seven days after transfection ...
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bioRxiv - Neuroscience 2021Quote: ... 1:1000 Myosin Heavy Chain 1 (05-716, Millipore), 1:5000 mCherry (ab205402 ...
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bioRxiv - Neuroscience 2023Quote: ... and NFH (neurofilament heavy chain, Cat#AB5539, Merck Millipore). We used Alexa Fluor 555nm labelled goat anti-mouse secondary antibody (Cat#A28180 ...
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bioRxiv - Bioengineering 2022Quote: ... mouse anti-myosin heavy chain (1 in 500, Sigma-Aldrich), rabbit anti-m-line titin (1 in 300 ...
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bioRxiv - Developmental Biology 2020Quote: ... Mouse anti-Myosin Heavy Chain (Sigma-Aldrich 05-716; 1:1000), Anti-beta III tubulin antibody (Sigma-Aldrich AB9354 ...
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bioRxiv - Biochemistry 2021Quote: ... ferritin (Sigma): R2 was 0.994 ...
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bioRxiv - Immunology 2022Quote: ... Antibody production was conducted by co-transfection of the heavy and light chain expression vectors into Human HEK293 suspension culture cells (Expi293F) using polyethylene mine (Sigma). Cells were subsequently cultured for 4 days before being pelleted by centrifugation ...
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bioRxiv - Cell Biology 2023Quote: ... The final concentration of ferritin (horse spleen ferritin, type I, Sigma) was 50 mg/ml ...
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bioRxiv - Immunology 2023Quote: ... Monoclonal antibodies were produced in 50 ml of 0.8×106 HEK293-6E cells by co-transfection of heavy chain (IgG1) and light chain (Igλ, Igκ) antibody expression plasmids with polyethylenimine (PEI; Sigma-Aldrich). Cells were maintained at 37°C and 5% CO2 in FreeStyle 293 Expression Medium (Thermo-Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... ferritin (equine spleen, Sigma), and ferrous (iron (II ...
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bioRxiv - Cell Biology 2023Quote: ... cationized ferritin (Sigma-Aldrich) was added in the saline solution at 0.25 mg/ml ...
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bioRxiv - Microbiology 2019Quote: ... a mouse monoclonal kinesin heavy chain antibody (MAB-1614, Millipore Sigma, at 1:1000), a mouse monoclonal KIF3A antibody (Clone K2.4 ...
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bioRxiv - Biochemistry 2021Quote: ... Cells were transfected with a 1:3 molar ratio of heavy chain and light chain expression vectors using the Expi293 Transfection kit (Sigma-Aldrich; L3287) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... light chain and the heavy chains of the included HLA types were expressed in bacterial Bl21 (DE3) pLysS strain (Novagen, cat#69451) and purified as inclusion bodies ...
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bioRxiv - Biochemistry 2021Quote: ... mouse monoclonal antibody against slow myosin heavy chain I (MYH1, UniProtKB Q5SX40. Sigma-Aldrich, M8421), rabbit monoclonal antibody against α-smooth muscle actin (α-SMA ...
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bioRxiv - Biophysics 2021Quote: A ferritin-agar phantom was prepared by adding horse spleen ferritin (Sigma, ref. F4503) to a 1.5% agar solution ...
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bioRxiv - Bioengineering 2020Quote: ... horse spleen ferritin nanocages (Sigma), or adeno-associated virus (empty capsids ...
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bioRxiv - Biochemistry 2021Quote: ... Equine Spleen Ferritin (Sigma F4503) without further purification was dissolved in buffer (3.5mM NH4SO4 and 100mMTris at pH7.4) ...
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bioRxiv - Cell Biology 2019Quote: ... horse spleen ferritin (Sigma-Aldrich, US) was deposited on a thin carbon film (∼5 nm ...
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bioRxiv - Systems Biology 2022Quote: ... Ferritin (F4503) was from Sigma-Aldrich.
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bioRxiv - Cell Biology 2020Quote: ... Fused cells (myotubes) were visualized using an antibody against Myosin heavy chain isoforms (MyHC) (MF20, Sigma-Aldrich). Primary antibodies were visualized with anti-rabbit or anti-mouse conjugated with Alexa 488 or Cy5 (ThermoFisher) ...
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bioRxiv - Biophysics 2021Quote: ... Atto647N-DOPE and polyclonal rabbit anti-clathrin heavy chain (dilution 1:100; Cat. Nr. HPA059143) was from Sigma. Monoclonal rabbit anti-EGFR (dilution 1:1000 ...
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bioRxiv - Bioengineering 2022Quote: ... The muscle construct was incubated with the primary antibody for myosin heavy chain (Sigma M4276, 1:400 dilution) for 2 hours at room temperature ...
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bioRxiv - Biochemistry 2021Quote: Crystals of ferritin (Sigma F4503 Lot# SLBV4372) were obtained using the microbatch method under oil[21] at room temperature (293 K) ...
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bioRxiv - Cell Biology 2023Quote: ... rabbit polyclonal antibody against non-muscle myosin heavy chain IIA (1:200 for immunofluorescence; Sigma-Aldrich; catalogue number M8064)
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bioRxiv - Biochemistry 2024Quote: ... Supernatants were harvested and Fab fragment was purified via the heavy chain His tag using affinity chromatography with Talon resin as per the manufacturer’s specifications (Sigma). Purified protein samples were dialysed into PBS as quantified by absorbance at 280nm.
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bioRxiv - Microbiology 2020Quote: ... anti-human IgM (μ-chain specific, Sigma, St. Louis, MO) was covalently coupled to NHS HP SpinTrap (GE Healthcare ...
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bioRxiv - Immunology 2023Quote: ... Goat anti-human IgG (γ-chain specific, A9544, Millipore-Sigma) or IgA (α-chain specific ...
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bioRxiv - Immunology 2023Quote: ... Goat anti-human IgG (γ-chain specific, A9544, Millipore-Sigma) or IgA (α-chain specific ...
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bioRxiv - Immunology 2020Quote: ... horse spleen ferritin (1mg/ml; Sigma, Gillingham, UK) was orally administered to aged mice via drinking water on days 0–2 and 7–9 ...
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bioRxiv - Biophysics 2019Quote: ... incubated again for 1 hour at 37°C with 1:400 mouse anti-heavy chain clathrin antibody (Sigma Aldrich, C1860) in PBS + 1% BSA and washed three times with PBS + 1% BSA ...
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bioRxiv - Neuroscience 2019Quote: ... for 15 min at room temperature and stained overnight with the primary antibodies at 4 °C (Kinesin heavy chain: Millipore MAB1614 1:50 ...
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bioRxiv - Cell Biology 2022Quote: ... A mouse anti-myosin heavy chain antibody was used as primary antibody (1:200; Sigma Aldrich, St. Louis, MO, USA) and Alexa 488 donkey anti-mouse IgG antibody as secondary antibody (Life Technologies ...
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bioRxiv - Biophysics 2020Quote: ... Polyclonal rabbit anti-clathrin heavy chain (Cat. Nr. SAB1410212) and rat monoclonal anti-β1-integrin (Cat. Nr. MAB1997) were from Sigma. Rabbit monoclonal anti-caveolin-1 (Cat ...
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bioRxiv - Microbiology 2021Quote: ... Plasmids encoding heavy and light chains were then co-transfected into the 293T cell line by the polyethylenimine method (408727; Sigma), and antibody were harvested for further study.
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and cloned into the mammalian cell expression vector as described previously.[50] Both the heavy and light chain constructs were co-transfected into Expi293 cell lines using transfection reagent PEI (Sigma). After 7 days of expression ...
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bioRxiv - Immunology 2021Quote: ... secondary antibody (goat anti-human γ-chain-specific whole IgG, α-chain-specific IgA, or μ-chain-specific IgM alkaline phosphatase conjugate, Sigma Aldrich, MO, US) was diluted 1:1000 and 50μl was added per well for 1 hour ...
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bioRxiv - Immunology 2021Quote: ... 1:3000 dilution of anti-human IgA (α-chain-specific) (Sigma) or 1:3000 dilution of anti-human IgM (μ-chain-specific ...
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bioRxiv - Microbiology 2020Quote: ... Cells were transfected at a density of 3 x 106 with equal amounts of heavy and light chain paired plasmids using polyethyleneimine (PEI, Sigma-Aldrich) at a ratio of 1:3 of total amount of plasmid to PEI ...
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bioRxiv - Immunology 2022Quote: Monoclonal antibody production was performed using 293-6E cells (National Research Council of Canada) by co-transfection of heavy and light chain expression plasmids using 25 kDa branched polyethylenimine (Sigma-Aldrich). Culture supernatants were harvested after an incubation period of 6-7 days at 37°C and 6% CO2 under constant shaking in FreeStyle Expression Medium supplemented with penicillin (20 U/ml ...
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bioRxiv - Neuroscience 2019Quote: ... cells were blocked-permeabilized in 5% gelatin from cold water fish skin and 0.1% triton X-100 for 1 h followed by incubation with primary antibodies (mouse anti-acetylated tubulin, and rabbit anti-Neurofilament heavy chain, Sigma-Aldrich) overnight at 4 °C ...
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bioRxiv - Genetics 2019Quote: ... and incubated overnight at 4 °C with primary anti-myosin heavy chains antibody (Monoclonal Anti-Myosin skeletal, Fast, Clone My-32, Mouse Ascities Fluid, M4276, Sigma-Aldrich) diluted (1:400 ...
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bioRxiv - Immunology 2022Quote: ... and the heavy and light chain sequences of the murine hybridomas were by obtained using the mouse Ig-primer set (69831; EMD Millipore) as described (83) ...
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bioRxiv - Physiology 2019Quote: ... Then one of the serial sections was incubated at RT in primary antibody against a slow isoform of the myosin heavy chains and the second serial section against a fast isoform of the myosin heavy chains (M8421, 1:5000; M4276; 1:600, respectively; Sigma-Aldrich, USA) for 1 h and incubated in PBS (3 × 5 min) ...
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bioRxiv - Molecular Biology 2019Quote: ... For myosin heavy chain, the membranes were incubated with 1:500 dilution of monoclonal anti-myosin (skeletal, fast) (M4276, Sigma-Aldrich, USA) and monoclonal anti-myosin (skeletal ...
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bioRxiv - Immunology 2021Quote: ... or 1:3000 dilution of anti-human IgM (μ-chain-specific) (Sigma) antibody subtypes and 1:6000 dilution of anti-human IgG1 ...
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bioRxiv - Physiology 2021Quote: ... ferritin (iron) from equine spleen – Type I standard (Sigma Aldrich) was diluted to 100 ...