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Citations for Millipore Sigma :
1 - 50 of 10000+ citations for Cell Isolation Optimizing System since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2022Quote: ... and following the best approaches for optimizing activity (Moyes et al., 2021): Solution A (4 units mL-1 lactate dehydrogenase (L2500, Sigma), 5 units mL-1 pyruvate kinase (P1506 ...
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bioRxiv - Immunology 2022Quote: Isolation of blood mononuclear cells was performed using density gradient centrifugation (ACCUSPIN System-Histopaque-1077; Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: RNA was extracted using the TRI Reagent isolation system (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: RNA from neurons or NPCs was extracted using the TRI Reagent isolation system (Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Total RNA isolation from cells was performed using Trizol (Sigma-Aldrich). Reverse transcription (RT ...
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bioRxiv - Microbiology 2022Quote: Cells were subcellularly fractionated using the ER isolation kit (Sigma, ER0100) as instructed by the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: Cells were directly lysed on cell cultures plate with 1 ml TriPure Isolation Reagent (Sigma). After adding 200 μl chloroform and centrifuging at 14,000 rpm for 15 min at 4°C ...
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bioRxiv - Pathology 2021Quote: Total RNA was extracted from cells with TriPure Isolation Reagent (Sigma-Aldrich) as previously described (62) ...
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bioRxiv - Cell Biology 2022Quote: ... cell homogenization took place in isolation buffer (225-mM mannitol (Sigma, M9647), 75-mM sucrose (Fisher S5-500) ...
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bioRxiv - Cancer Biology 2024Quote: ... TriPure Isolation Reagent (Sigma) was used to lyse cells directly on the cell culture plates ...
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bioRxiv - Molecular Biology 2020Quote: Isolation of total mRNA from cells was performed with TRI-reagent (Sigma-Aldrich), and 1 µg of extracted RNA was reverse transcribed into cDNA according to manufacturer’s instructions using the QuantiTect Reverse Transcription Kit (QIAGEN ...
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bioRxiv - Immunology 2023Quote: Total RNA was isolated from cells using TriPure Isolation Reagent from Sigma-Aldrich using the manufacturer’s instructions (Cat ...
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bioRxiv - Developmental Biology 2019Quote: Mitochondria isolation was performed by Mitochondrial Isolation Kit (Sigma Aldrich, Cat# MITOISO1-1KT) according to the manual’s instruction ...
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bioRxiv - Plant Biology 2021Quote: ... followed by nuclear isolation with the Plant Nuclei Isolation/Extraction Kit (Sigma-Aldrich) and sonication with a Bioruptor (Diagenode ...
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bioRxiv - Developmental Biology 2021Quote: ... through a nuclear isolation protocol based on the Nuclei EZ isolation kit (Sigma) but optimized for zebrafish tissue ...
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bioRxiv - Microbiology 2020Quote: ... Whole genome isolation was performed using GenElute bacterial DNA isolation kit (Sigma-Aldrich). Paired-end sequencing was done by using the Illumina Miseq platform ...
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bioRxiv - Microbiology 2020Quote: RNA was isolated from cell culture supernatants and cell lysates using the TriPure Isolation Reagent (Sigma-Aldrich). Equine arteritis virus (EAV ...
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bioRxiv - Immunology 2020Quote: ... RNA isolation was then performed according to the isolation protocol from TRIreagent supplier (SIGMA). In brief ...
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bioRxiv - Developmental Biology 2023Quote: ... Nuclei isolation was performed with the Nuclei Isolation Kit: Nuclei EZ Prep (Sigma-Aldrich) with some modifications ...
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bioRxiv - Physiology 2023Quote: Total RNA from tissue and cells was extracted using Tripure Isolation Reagent (Sigma-Aldrich) using Bullet Blender Homogenizer (Next Advance ...
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bioRxiv - Developmental Biology 2023Quote: Total RNA was isolated from cells or tissues using Tripure Isolation Reagent (Sigma-Aldrich) and Bullet Blender Homogenizer (Next Advance ...
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bioRxiv - Biochemistry 2023Quote: ... the Peroxisome Isolation kit (Sigma) was used to isolate peroxisomes from primary hepatocytes by referring to the protocol provided by Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... Pseudomonas Isolation Agar (PIA, Sigma) was used to specifically select P ...
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bioRxiv - Cell Biology 2023Quote: Golgi isolation Kit (Sigma GL0010) was used with some modifications for cultured Cells Golgi subcellular fractionation ...
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bioRxiv - Neuroscience 2019Quote: ... Nuclei isolation was carried out using the Nuclei Isolation Kit: Nuclei EZ Prep (Sigma, #NUC101) as described49 ...
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bioRxiv - Neuroscience 2019Quote: ... Nuclei isolation was carried out using the Nuclei Isolation Kit: Nuclei EZ Prep (Sigma, #NUC101) as described in 9 ...
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bioRxiv - Neuroscience 2022Quote: ... The nucleus isolation was carried out by Nuclei Isolation Kit: Nuclei EZ Prep (Sigma, NUC101). Briefly ...
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bioRxiv - Biophysics 2019Quote: ... Dead cells were removed on day 6 after T cell isolation through density-dependent gradient centrifugation (Histopaque 1119, Sigma) and experiments were conducted between day 7 and day 9 after isolation.
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bioRxiv - Genetics 2020Quote: ... For RNA isolation collected cells were washed with PBS and resuspended in TRI reagent (Sigma). For protein extraction cells were resuspended in PBS containing 0.1% NP-40 and protease inhibitors and incubated for 30 minutes in 37 °C in the presence of 250 U of Viscolase (A&A Biotechnology).
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bioRxiv - Immunology 2021Quote: ... sample replicates for single cell isolation were digested with 100U/ml of Collagenase IV (Sigma), 50 U/ml of Benzonase (Sigma ...
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bioRxiv - Biophysics 2020Quote: All the cell-isolation and culture materials were obtained from Sigma-Aldrich (St. Louis, MO) and Fisher Scientific (Hampton ...
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bioRxiv - Molecular Biology 2020Quote: Lysosomes were purified from 293T cells using a lysosome isolation kit (Sigma, Cat# LYSISO1-1KT). According the protocol ...
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bioRxiv - Developmental Biology 2019Quote: ... FACS-sorted cells were pelleted and re-suspended in nuclear isolation buffer (Sigma #NUC101-1KT). Depending on input size ...
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bioRxiv - Immunology 2020Quote: The isolation of cells from peribronchial lymph nodes was done using a Collagenase D (Sigma ) and DNAse I (BioConcept ...
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bioRxiv - Cell Biology 2021Quote: ... Each cell pellet was resuspended in 100uL of Nuclei EZ Prep Isolation buffer (Sigma, NUC101) and placed on ice for 5 minutes before being centrifuged (5 minutes at 500 x G) ...
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bioRxiv - Cell Biology 2024Quote: ... Mitochondrial isolation was carried out by differential centrifugation using the Mitochondrial Isolation Kit (MITOISO2; Sigma-Aldrich) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Sigma mitochondrial isolation kit (Sigma, MITOISO1) was used to extract mitochondria and perform the JC-1 assay according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2023Quote: ... and Golgi isolation kit (Sigma, GL0010). Pierce™ cell surface protein biotinylation and isolation kit (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2021Quote: ... Dead cells were removed six days after T-cell isolation with a density-dependent gradient centrifugation step (Histopaque 1119, Sigma). Antigen-experienced T-cells were used for experiments on day 7 – 9.
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bioRxiv - Biophysics 2020Quote: ... Dead cells were removed on day 6 after T-cell isolation by means of density-dependent gradient centrifugation (Histopaque 1119, Sigma). Antigen-experienced T-cells were used for experiments on day 7 – 9.
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bioRxiv - Immunology 2023Quote: ... EasySep™ Human NK Cell Isolation Kit) and were frozen down in CS10 CryoStor cell cryopreservation media media (Sigma-Aldrich).
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bioRxiv - Microbiology 2020Quote: Cells were washed in PBS and miRNA was extracted using the mirPremier microRNA Isolation Kit (Sigma) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2020Quote: ... prior to the isolation cells were treated for 2 hrs with 500 nM BafA (EMD Millipore) or the corresponding DMSO volume.
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bioRxiv - Microbiology 2022Quote: Cells were harvested and RNA was isolated using mammalian total RNA isolation kit (Sigma, RTN70-1KT). Following the Superscript III reverse transcriptase protocol (Invitrogen) ...
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bioRxiv - Genomics 2021Quote: ... HeLa cells for nucleus isolation were scraped in 2mL of EZ lysis buffer (Sigma Aldrich N3408) and moved to a 15mL falcon tube for nucleus extraction.
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bioRxiv - Cancer Biology 2021Quote: ... Cell pellets were snap frozen in liquid nitrogen and nuclei were extracted from cell pellets (Sigma Nuclei Isolation Kit #NUC-101). Chromatin was fragmented in sonication buffer (50mM HEPES pH 7.5 ...
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bioRxiv - Microbiology 2021Quote: ... and on Pseudomonas isolation agar (Sigma-Aldrich) for C ...
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bioRxiv - Microbiology 2021Quote: ... and on Pseudomonas isolation agar (Sigma-Aldrich) for C ...
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bioRxiv - Microbiology 2019Quote: Following isolation of total RNA from N2A cells and mouse brain samples using Tri reagent (Sigma-Aldrich), 250 ng of RNA was reverse transcribed with the help of Verso cDNA synthesis kit (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2023Quote: ... After isolation astrocytes were plated in 15cm cell culture plates coated with poly-D-lysine (Sigma P6407) at 3 million cells/plate and maintained at 37°C and 10% CO2 ...