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Citations for Millipore Sigma :
1 - 50 of 10000+ citations for Borrelia burgdorferi sensu stricto B31 CRASP 2 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... burgdorferi B31 (PA003) spirochetes were diluted into BSK-II or BSK-II +10mM spermidine (Sigma Aldrich, Catalog #S2626-1G) adjusted to pH 7.8 ...
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bioRxiv - Microbiology 2019Quote: B. burgdorferi strain B31 was cultured in BSK-H medium (HiMedia Laboratories Pvt. Ltd.) with 6% rabbit serum (Sigma-Aldrich, St. Louis, MO, USA). All culture medium was filter-sterilized by 0.2 µm filter ...
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bioRxiv - Microbiology 2021Quote: ... burgdorferi N40 were grown at 33°C in Barbour-Stoenner-Kelly H (BSK-H) complete medium (Sigma-Aldrich, #B8291) with 6% rabbit serum ...
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bioRxiv - Neuroscience 2021Quote: ... Microtubule-Associated Protein 2 (MAP-2) (Merck Millipore Burlington ...
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bioRxiv - Microbiology 2023Quote: ... burgdorferi at an MOI of 50 for 18h alone or in the presence of 50mM N-acetyl cysteine (NAC; Sigma, A7250). Cells were collected in RIPA buffer (for immunoblotting ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were separated in 2% SDS (Sigma), 40% acrylamide/bis (Biorad ...
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bioRxiv - Molecular Biology 2020Quote: Proteins were expressed in Rosetta 2 cells (EMD Millipore) grown in LB at 37°C and induced at an OD600 of 0.7 with 0.5 mM IPTG for 16 hours at 18°C ...
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bioRxiv - Bioengineering 2019Quote: ... microtubule associated protein 2 (MAP2) (1:500, Sigma-Aldrich). The cells were subsequently washed 6 x 5 min each with PBS/0,1% Triton-X 100 ...
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bioRxiv - Cell Biology 2022Quote: ... Proteins were expressed in Escherichia coli Rosetta 2 (Novagen) in LB media ...
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bioRxiv - Developmental Biology 2020Quote: ... anti-microtubule associated protein 2 (MAP2, 1:200, Millipore), mouse monoclonal anti-ankyrinG (AnkG ...
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bioRxiv - Neuroscience 2022Quote: ... and stained with microtubule-associated protein 2 (MAP 2) antibody (1:500, Sigma aldrich M9942), ALK (1:500 ...
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bioRxiv - Biochemistry 2019Quote: ... 2 µg of Rab protein were mixed with 5x SYPRO® orange protein gel stain (Sigma) in a volume of 20 µl ...
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bioRxiv - Biochemistry 2021Quote: ... programed for uniform mixing as previously described at a controlled temperature and modified with online automated desalting.13 Proteins were denatured in a solution of 35% v/v 2-2-2 Trifluoroethanol (TFE, Sigma), 40 mM Dithiothreitol (DTT ...
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bioRxiv - Biochemistry 2021Quote: ... Proteins were alkylated using 2-chloroacetamide (C0267-100G, Sigma-Aldrich) in elution buffer (100 mM Tris-HCl pH 7.5 ...
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bioRxiv - Neuroscience 2020Quote: ... mouse anti-microtubule-associated protein 2 (Map2, 1:250, Sigma), rabbit anti-glial fibrillary acidic protein (GFAP ...
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bioRxiv - Molecular Biology 2020Quote: ... containing protein phosphatase inhibitor cocktail 1 and 2 (Sigma-Aldrich) and protease inhibitors (Sigma-Aldrich) ...
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bioRxiv - Bioengineering 2019Quote: ... 20μg protein was incubated in 10% 2-Mercaptoethanol (Sigma-Aldrich) in laemmli loading buffer (Bio-Rad ...
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bioRxiv - Biochemistry 2021Quote: ... protein expression was induced by 2 μg/mL doxycycline (SIGMA) for 48 h ...
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bioRxiv - Developmental Biology 2021Quote: ... Proteins were reduced with tris-2-carboxyethyl phosphine (ΤCEP, Sigma) for 1h at 60°C at a final concentration of 5mM ...
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bioRxiv - Developmental Biology 2022Quote: ... 2 μM M3814 (DNA-dependent protein kinase inhibitor) (Sigma-Aldrich) was added to the cell media for 72 hr to repress non-homologous end joining DNA repair (Riesenberg et al. ...
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bioRxiv - Neuroscience 2022Quote: ... rabbit anti-microtubule associated protein-2 (MAP2) (Millipore Sigma AB5622), and mouse anti-TUBB3/β3 tubulin (Novus Biologicals ...
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bioRxiv - Immunology 2023Quote: ... bound proteins were eluted in 2× Laemmli buffer (Sigma-Aldrich), boiled for 5 min at 95°C and separated on a 4-12% NuPAGE Novex Bis-Tris precast gel (Thermo Scientific).
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bioRxiv - Molecular Biology 2023Quote: ... The protein was expressed E.coli strain Rosetta 2 (DE3) (Novagen). The cells were grown at 37 °C in Luria-Bertani medium (LB ...
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bioRxiv - Plant Biology 2020Quote: ... 2 mM EDTA and 1 % [v/v] protein phosphatase inhibitor cocktail 2 and 3 (Sigma-Aldrich)) were added at a concentration of 2 mL/g tissue powder ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein sample (400 μg) was reduced by adding 2 μl of Tris(2-carboxyethyl)phosphine (Sigma) and incubating samples at 60 °C for 1 h ...
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bioRxiv - Biochemistry 2020Quote: ... Proteins were co-expressed in Rosetta 2(DE3)pLysS Ecoli (Novagen). Cells were grown at 37°C in 2XYT media to an OD600 of 0.8 ...
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bioRxiv - Microbiology 2021Quote: ... Protein extraction was performed in the presence of 2% SDS (Sigma) and 4 M acetic acid (Fisher ...
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bioRxiv - Cell Biology 2023Quote: ... Protein samples were denatured with 5 % v/v 2-mercaptoethanol (Sigma) for 5 min at 95 °C before they were loaded in Bolt Bis-Tris gels (Thermo Fisher) ...
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bioRxiv - Biochemistry 2023Quote: ... 25 µg of protein was reduced with 2 mM dithiothreitol (Sigma) for 60 minutes at 55 °C ...
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bioRxiv - Biochemistry 2020Quote: ... Solubilized protein was then loaded onto 2 mL M2 affinity matrix (Sigma) previously equilibrated with wash buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Biophysics 2021Quote: ... Protein expression was performed in Origami™ 2 (DE3) competent cells (Novagen) using LB medium ...
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bioRxiv - Microbiology 2020Quote: ... Protein was reduced with tris (2-carboxyethyl) phosphine (Sigma-Aldrich, MO, USA) and alkylated with methylmethanethiosulphonate (MMTS ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... anti-microtubule-associated protein 2 (MAP2) Alexa 555 (1:500, MAB3418A5; Millipore), antimicrotubule-associated protein 2 (MAP2 ...
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bioRxiv - Immunology 2021Quote: ... Non-specific protein was blocked with 2 % normal donkey serum (Sigma Aldrich) in PBS containing 0.05 % Tween-20 and 1 % BSA ...
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bioRxiv - Microbiology 2022Quote: The MLB1 CPNTD protein was produced in Rosetta 2 (DE3) cells (Novagen) cultured in 2×YT media with overnight expression at 18 °C induced with 0.4 mM IPTG ...
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bioRxiv - Neuroscience 2023Quote: ... the proteins were reduced with 10mM tris(2-carboxyethyl)-phosphine (TCEP) (Sigma) for 20 min ...
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bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 spike protein (S1) (#E5S3V) and GAPDH (G9545, Sigma-Aldrich) were incubated overnight at 4 °C in TBS-T ...
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bioRxiv - Cancer Biology 2023Quote: ... for 2 h on ice before mixing with protein G-Sepharose (Millipore). The antibody complex was washed three times in lysis buffer and once in Tris-buffered saline (TBS) ...
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bioRxiv - Biophysics 2020Quote: ... For reduction and alkylation of the proteins, proteins were incubated with SDC buffer (1% Sodiumdeoxycholate, 40nmM 2-Cloroacetamide (Sigma-Aldrich), 10 mM tris(2-carboxyethyl ...
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Optimization and deoptimization of codons in SARS-CoV-2 and the implications for vaccine developmentbioRxiv - Evolutionary Biology 2022Quote: ... About 2×106 cells of HEK293T cells were transfected with 2 ug of plasmids encoding S proteins using polyetherimide (PEI; Sigma). After 16 hours of incubation ...
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bioRxiv - Cell Biology 2019Quote: ... 2 μl lambda protein phosphatase (800 U) and buffer components as instructed (Sigma) and the reaction was terminated after 30 min at 30 °C ...
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bioRxiv - Neuroscience 2019Quote: ... anti-microtubule associated protein 2 (MAP2, 1:500, mouse monoclonal, Sigma, St.Louis, MO), GluN2B (1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... Proteins were reduced with 10 mM Tris(2-carboxyethyl)phosphine (TCEP; Sigma-Aldrich) for 1 h at room temperature followed by alkylation of cysteine residue side chain thiol groups with iodoacetamide (Sigma-Aldrich ...
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bioRxiv - Biophysics 2020Quote: ... The CbAgo protein was expressed in E.coli Bl21(DE3) Rosetta™ 2 (Novagen). Cultures were grown at 37 °C in LB medium containing 50 μg ml−1 kanamycin and 34 μg ml−1 chloramphenicol till an OD600 nm of 0.7 was reached ...
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bioRxiv - Biophysics 2019Quote: ... The CbAgo protein was expressed in E.coli Bl21(DE3) Rosetta™ 2 (Novagen). Cultures were grown at 37°C in LB medium containing 50μa.g ml-1 kanamycin and 34μg ml-1 chloramphenicol till an ODóOOnm of 0.7 was reached ...
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bioRxiv - Biochemistry 2019Quote: ... and 1% NP-40 containing protein phosphatase inhibitor cocktail 1 and 2 (Sigma) and protease inhibitors (Sigma)] ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... antimicrotubule-associated protein 2 (MAP2) Alexa Fluor 647 (1:500, NB120-11267AF647; Millipore). Cell nuclei were stained by using Hoescht 33342 (2 μg/mL ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were subsequently incubated for 2 h with Protein-G–Sepharose (Sigma-Aldrich). After 3 washes with lysis buffer ...
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bioRxiv - Biophysics 2023Quote: ... recombinant Rad26 protein was expressed in Escherichia coli strain Rosetta 2(DE3) (Novagen) and purified by Ni-NTA agarose (Qiagen) ...
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bioRxiv - Developmental Biology 2019Quote: ... 2 mg of protein lysate were incubated for 3 h with 2 μg of α-FLAG M2® antibody (Sigma-Aldrich) pre-coupled to 20 μl of rotein G Dynabeads® (Thermo Fisher Scientific ...