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Citations for Millipore Sigma :
1 - 50 of 10000+ citations for Anti NET SAP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: Anti-β-tubulin antibodies (75 μg ml−1, SAP.4G5, Sigma-Aldrich) in BRB80 was flushed into the flow cell and incubated for 5 min to allow for hydrophobic interaction-based absorption to the surface ...
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bioRxiv - Neuroscience 2023Quote: ... mouse monoclonal anti-β-Tubulin antibody (1:10,000; SAP.4G5 Sigma-Aldrich); rabbit monoclonal anti-TBP (1:2000 ...
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bioRxiv - Cancer Biology 2023Quote: ... or seed saporin (SAP) (Sigma-Aldrich) in 1 M Na2CO3 (pH 11.5 ...
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bioRxiv - Immunology 2019Quote: ... NETs were evidenced by DAPI (Sigma-Aldrich), rabbit polyclonal anti-MPO antibody (1:50 for in vitro immunofluorescence ...
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bioRxiv - Cell Biology 2024Quote: Mice received bilateral injections of either unconjugated blank-SAP or SAP conjugated to an antibody directed to DBH (DBH-SAP; 42 ng/200 nL, pH=7.4; MAB394; Millipore, Temecula, CA, USA), using a Hamilton syringe directed toward the nTS (0.1 mm rostral to Obex ...
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bioRxiv - Biophysics 2020Quote: ... filled and incubated successively with anti β-tubulin I (monoclonal antibody SAP.4G5 from Sigma in PEM), Pluronic F-127 (1 % in PEM) ...
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bioRxiv - Biophysics 2020Quote: ... filled and incubated successively with anti β-tubulin I (monoclonal antibody SAP.4G5 from Sigma in PEM) for 15-20 min ...
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bioRxiv - Neuroscience 2023Quote: ... They were then incubated with mouse anti-NET primary antibody (1:1000, ref AMAB91116-100UL, Sigma Aldrich) diluted in the blocking solution during 72h at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... was acquired from MedChemExpress and XRP44X (Ras-Net-Elk-3 inhibitor) from Sigma-Aldrich. The drugs were reconstituted in DMSO ...
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bioRxiv - Biophysics 2022Quote: ... A nylon net with 20 μm pore size (NY2002500, Merck Millipore) was placed on the bottom of a 10 mL syringe (BD307736 ...
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bioRxiv - Bioengineering 2023Quote: ... NETs were incubated in media containing 5 mM PMA (Sigma-Aldrich) for 4 hr ...
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bioRxiv - Immunology 2021Quote: ... the wells were incubated with streptavidin-alkaline phosphatase (SAP) (Sigma Aldrich, USA) for one hour at room temperature ...
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bioRxiv - Immunology 2020Quote: ... NET solutions were added with protease inhibitors and 1% Benzonase Nuclease (Sigma) and incubated at 37°C for 20 min to remove nucleic acids ...
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bioRxiv - Microbiology 2022Quote: ... Stained cells were passed through a 0.41 µm Nylon Net Filter (Millipore) and analyzed using an LSRII Flow Cytometer (BD Biosciences ...
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bioRxiv - Cell Biology 2019Quote: ... washed with PBS and permeabilized with SAP buffer [0.2% Saponin (Sigma-Aldrich S4521), 0.2% Gelatin (Himedia Laboratories TC041 ...
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bioRxiv - Neuroscience 2022Quote: ... cells were filtered using a 20 μm nylon net (Merck Millipore, Darmstadt, Germany), followed by centrifugation at 1500 rpm for 5 mins at 4°C ...
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bioRxiv - Physiology 2023Quote: ... gastrocnemius muscles were placed on 20 μM nylon net filters (Millipore, cat# NY2004700) and centrifuged (10 min ...
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bioRxiv - Molecular Biology 2023Quote: ... The tissue debris was removed by filtration with 60 μm nylon net (Millipore). Nuclei were collected and washed with RIPA buffer at 4 °C (150 mM NaCl ...
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bioRxiv - Molecular Biology 2021Quote: ... NET formation was induced with 10 nM phorbol 12-myristate 13-acetate (PMA; Sigma) for 4h at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... The supernatant was sequentially passed through three 100 μm nylon net filters (Millipore NY1H02500), followed by one 5 μm filter (Millipore SMWP013000) ...
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bioRxiv - Cancer Biology 2022Quote: ... size exclusion columns were prepared with an 11 µm nylon net filter (NY1102500, Millipore) placed in the bottom of a 10 mL syringe (BD 302995) ...
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bioRxiv - Neuroscience 2022Quote: ... Perineuronal nets were stained with biotinylated Wisteria floribunda agglutinin (WFA, Sigma-Aldrich L1516, RRID:AB_2620171). Biocytin and biotinylated WFA were both visualized using streptavidin conjugated to Alexa Fluor 647 (Invitrogen S21374 ...
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bioRxiv - Immunology 2022Quote: ... The stimuli used to induce NET formation were phorbol 12-myristate 13-acetate (PMA, Sigma), PVL (equal amounts of S ...
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bioRxiv - Plant Biology 2019Quote: ... the filtered inflorescence sap was directly used as reducing agent to synthesize AgNPs using AgNO3 (SIGMA) as precursor ...
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bioRxiv - Microbiology 2020Quote: ... The microbial DNA was purified using the Exo-sap DNA Clean-Up Kit (Sigma-Aldrich, Israel) using 5 L aliquot of the supernatant ...
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bioRxiv - Microbiology 2021Quote: ... 5 µl of 200 µM drug solutions dissolved in net DMSO (Sigma Aldrich: Cat no. D5879) were pipetted into the 96-well plate using a multichannel pipette (Gilson) ...
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bioRxiv - Microbiology 2021Quote: ... The rest of the protozoa underwent consecutive filtration using nylon net filters (Merck Millipore, Darmstadt, Germany) of different sizes (i.e. ...
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bioRxiv - Microbiology 2020Quote: ... at 37°C) and filtered through a 100 μm pore size nylon net filter (Millipore, U.S.A). The filtered samples from each cow were then aliquoted into three tubes ...
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bioRxiv - Genomics 2024Quote: ... Steriflip vacuum-driven filtration system with 20 µm nylon net filter (Merck Millipore, Cat. no. SCNY00020), 40 µm polypropylene framed cell strainers (Biologix ...
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bioRxiv - Immunology 2024Quote: ... TEER values (Ω * cm2) were calculated as net resistance * membrane area (0.33cm2 for 24-well inserts, Millipore). Only samples with TEER > 200 Ω*cm2 on day 10 were used for stimulation experiments.
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bioRxiv - Cell Biology 2020Quote: ... cells were harvested with 500 µl of NET buffer (150 mM NaCl, 50 mM Tris pH7.5, 1 mM EDTA and protease inhibitor cocktail from Sigma). Cells were kept on ice for 15 minutes and lysed by 20 times up and down pipetting ...
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bioRxiv - Plant Biology 2021Quote: Interactions of shikonin with cell sap was analyzed by normal phase TLC (silica gel 60 F254, Merck Millipore, Darmstadt, Germany). In addition ...
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bioRxiv - Cancer Biology 2023Quote: ... the unencapsulated FITC-dextran or FITC-SAP was removed by washing and filtration using Amicon Ultra centrifugal filters (100 K device, Merck Millipore). The incorporation of FITC-dextran and FITC-SAP into the nsEVs was confirmed by spectrofluometer analysis (FP-6200 ...
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bioRxiv - Bioengineering 2021Quote: ... Supernatant was replaced with fresh trehalose-HEPES-ACSF and the resuspended cell pellet was strained with a 20 μm nylon net filter (NY2004700, Millipore). After resuspension in trehalose-HEPES-ACSF ...
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bioRxiv - Microbiology 2020Quote: ... each sample was filtered through a net (size 27 μm) and then filtered through nitrocellulose filter with a pore size of 0.22 μm (Millipore, France), and the material from the filter was transferred to sterile flasks with 20 mL of lysis buffer (40 mmol L-1 EDTA ...
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bioRxiv - Microbiology 2020Quote: ... Up to 6.9 L of seawater from each sampling depth were filtered through a series of 20 μm Nylon Net filters (Millipore), 5 μm polycarbonate membrane filters (Millipore) ...
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bioRxiv - Biochemistry 2019Quote: ... The digested samples were then syringe-filtered through 140 μm and 60 μm nylon net filters installed in Swinnex filter holders (Millipore), centrifuged at 16,000×g for 5 min ...
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bioRxiv - Physiology 2023Quote: ... The pelleted cells of the cardiac myocyte fraction were resuspended in ice-cold 7 mL Perfusion Buffer and applied onto a 20-µm nylon net membrane (Millipore, SCNY00020). Trapped cells on the membrane were collected with 7 mL Perfusion Buffer ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: The zebrafish (approximately 800 fish) were caught using a net and anesthetized with tricaine spray (Sigma-Aldrich, St. Louis, MO, USA) on an ice plate ...
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bioRxiv - Neuroscience 2024Quote: ... aCSF and 192IgG-SAP groups received intraperitoneal (ip) injections of WIN55,212-2 (0.5 mg/kg or 3 mg/kg) (Sigma-Aldrich, St. Louis, MO, USA) or vehicle solution (1:1:18 ...
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bioRxiv - Genomics 2020Quote: Approximately 5 × 106 cells from the overnight incubation with the primary/secondary antibody-antibody complex was stringently washed with NET-2 buffer then denatured in a DL-Dithiothreitol (DTT) (Sigma-Aldrich D9779), NuPAGE™ LDS Sample Buffer (4X ...
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bioRxiv - Cell Biology 2022Quote: ... Size-exclusion chromatography (SEC) was performed by using a nylon net with 20 µm pore size (NY2002500, Merck Millipore, Billerica, Massachusetts, USA) was placed on the bottom of a 10 mL syringe (BD Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... beads washed five times with pre-chilled NET resuspension buffer and eluted under native conditions by competition using 3x FLAG peptide (Millipore Sigma, F4799). Samples were boiled in the Laemmli SDS sample buffer (Thermofisher ...
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bioRxiv - Genomics 2021Quote: ... 20 and 5 μm filters (nylon 40 μm cell strainer from BIOLOGIX; 20 μm net ring from Pharmacia Fine chemicals; Whatman® Puradisc 13 syringe filters 5 μm from SIGMA-ALDRICH®). Next ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The chromatin was incubated with an anti-histone PTM antibody (anti-H4K20me3, anti-H3K4me3, and anti-H3K9ac, Cell Signal Technology; anti-H3K27ac, Millipore; anti-H3K36me3, Abcam) overnight at 4°C and the immunoprecipitation carried out using Dynabeads protein A and Dynabeads protein G ...
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bioRxiv - Cell Biology 2023Quote: ... anti-rabbit anti-chicken and anti-sheep were purchased from Sigma.
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bioRxiv - Cell Biology 2019Quote: ... anti-MIC27 AntI-MIC27 (Sigma, cat# HPA000612), anti-GAPDH (ProteinTech ...
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bioRxiv - Physiology 2020Quote: ... anti-Actin and anti-Tubulin (Sigma-Aldrich).
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bioRxiv - Cell Biology 2021Quote: ... anti-HA and anti-VSV-G (Sigma cat ...
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bioRxiv - Cancer Biology 2023Quote: Anti-Rabbit IgG/Anti-mouse IgG (Millipore) was used for isotype control.