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Citations for Millipore Sigma :
4751 - 4800 of 4915 citations for Recombinant Human CTLA4 Fc His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... human TLR4/CD14/MD2 or non-transfected (null-1) were cultured and maintained using DMEM GlutaMAX™ containing 10 % FBS (Sigma-Aldrich), 1 % P/S ...
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bioRxiv - Bioengineering 2021Quote: ... supplemented with 2 mM L-glutamine and 1% Antibiotic -Antimycotic (Life Tech, UK) with 20 ng/mL human epidermal growth factor (hEGF, Sigma-Aldrich, UK), 20 ng/mL human basic fibroblast growth factor (hbFGF ...
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bioRxiv - Cell Biology 2020Quote: A MilliPlex MILLIPLEX® MAP Human Cytokine/Chemokine/Growth Factor Panel A-Immunology Multiplex Assay (HCYTA-60K-13, Millipore Sigma, Burlington, Massachusetts) was run on a Luminex 200 Instrument (LX200-XPON-RUO ...
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bioRxiv - Physiology 2021Quote: Ion channel genes were expressed in human embryonic kidney (HEK) tsA cells obtained from two sources (Prof. R. Horn, Jefferson University, Philadelphia, PA and Sigma-Aldrich #96121229) as described previously (20) ...
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bioRxiv - Neuroscience 2021Quote: ... This iPS cell line was obtained by reprogrammation of a commercial fibroblast cell line (FibroGRO™ Xeno-Free Human Foreskin Fibroblasts, #SCC058, Merck Millipore, Germany). The patient hiPSC cell line was obtained by reprogrammation of skin primary fibroblasts from the female patient II.2 ...
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bioRxiv - Neuroscience 2020Quote: ... medium was changed to Nb/B27 (Mouse) or DDM/B27+Nb/B27 (Human) medium with following chemical compounds: M1 (50µM, Sigma-Aldrich, Cat#SML0629), Mdivi-1 (50µM ...
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bioRxiv - Genetics 2021Quote: ... HSPCs were transferred into erythroid differentiation medium (EDM) consisting of IMDM (LIFE, 12440061) supplemented with 330 μg ml-holo-human transferrin (Sigma, T0665-1G), 10 μg ml-recombinant human insulin (Sigma ...
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bioRxiv - Cancer Biology 2021Quote: ... Either DNA from the BRCA1-methylated HCC38 TNBC cell line or CpGenome™ Human Methylated and Non-Methylated DNA Standards (Sigma-Aldrich) were included in each bisulfite conversion reaction as controls ...
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bioRxiv - Immunology 2020Quote: ... Human breast adenocarcinoma cell line (MCF-7) were obtained (ATCC, Manassas, VA, USA) and maintained in DMEM-F12 (Sigma-Aldrich, MO, USA) with 10% fetal bovine serum ...
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bioRxiv - Neuroscience 2021Quote: ... This antiserum detects human α-CGRP and β-CGRP but does not cross-react with any other peptide (data supplied by Sigma). We used a rabbit polyclonal antibody (1:1000 ...
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bioRxiv - Microbiology 2020Quote: ... or 293CD4 cells (293 cells constitutively expressing human CD4) were grown in Dulbecco’s modified Eagle’s medium (DMEM, Sigma, St. Louis, MO, USA) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Microbiology 2021Quote: Pig kidney cell line PK-15 and human kidney 293T cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) (Sigma, St. Louis, MO, USA) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2020Quote: Cells were stained directly without fixation in FACS buffer (PBS supplemented to 1% FBS, 10 μg/mL human-IgG (Sigma, #I8640-100MG), and 0.01% Sodium azide ...
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bioRxiv - Biochemistry 2022Quote: ... neutralizing monoclonal antibodies against human catalase (clone CAT-505, mouse, IgG1) and control antibodies directed against laminin were obtained from Sigma (Schnelldorf, Germany).
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bioRxiv - Bioengineering 2022Quote: ... the mononuclear cell fraction of rat bone marrow or human peripheral blood was obtained by layering the cell suspension over Histopaque®-1083 (density: 1.083 g/mL, Sigma, ON, Canada) or Ficoll (density ...
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bioRxiv - Immunology 2022Quote: ... The moANCA518 binding to the antigen was detected after incubation for 1 hour at RT of alkaline phosphatase conjugated goat anti-human IgG (Sigma, A-9544) antibody in a 1:10,000 dilution in TBS (20 mM Tris ...
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bioRxiv - Microbiology 2022Quote: ... VeroE6/TMPRSS2 cells (VeroE6 cells stably expressing human TMPRSS2; JCRB1819)15 were maintained in DMEM (low glucose) (Sigma-aldrich, Cat# D6046-500ML) containing 10% FBS ...
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bioRxiv - Bioengineering 2024Quote: ... cells were seeded at 1.5 x103/cm2 on 13 mm coverslips in 24 well-plates and cultured in DMEM containing 2% with human AB serum (HS; Sigma Aldrich, H4522) in a 5% humidified CO2 atmosphere at 37°C ...
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bioRxiv - Genetics 2024Quote: ... This iPS cell line was obtained by reprogramming of a commercial fibroblast cell line (FibroGRO™ Xeno-Free Human Foreskin Fibroblasts, #SCC058, Merck Millipore, Germany). Patients’ hiPSC cell lines were obtained by reprogramming of skin primary fibroblasts from ...
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bioRxiv - Microbiology 2024Quote: ... cells were incubated with CR3022 anti-S antibody (1 ug/mL)128 overnight followed by anti-human IgG-HRP-conjugated goat secondary antibody (Sigma, 1:500) for 2 h ...
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bioRxiv - Immunology 2024Quote: ... and developed by detecting bound IgG or IgA with appropriate fluorochrome-labelled secondary antibodies: 1 µg/ml goat anti-human IgG-Cy3 (Sigma, Cat# C2571) or goat anti-human IgA-Cy5 (Jackson ImmunoResearch ...
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bioRxiv - Genetics 2024Quote: The human malignant papillary serous carcinoma cell line OV-90 (Gralewska et al, 2021) was cultured in MCDB 105 (Sigma Aldrich, 117) and Medium 199 (Sigma Aldrich ...
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bioRxiv - Immunology 2024Quote: Phagocytosis capacity was assessed for 500’000 PBMCs per well in a 24-well plate in 500µl medium (RPMI with 10% FBS, 1% penicillin-streptomycin) supplemented with 20% of human AB serum (Sigma-Aldrich Cat.# H3667). PHrodo E ...
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bioRxiv - Microbiology 2024Quote: ... Primary human monocyte-derived macrophages (hMDMs) were seeded at 1×105 cells per well in RPMI-1640 supplemented with human serum (10% v/v) (Sigma, cat# H4522) for 16 hours prior to infection ...
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bioRxiv - Physiology 2023Quote: Frozen aliquots of tsA-201 cells (a variant of human embryonic kidney HEK cells) were obtained from Sigma-Aldrich (St. Louis, MO) and stored in liquid nitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... Cytokine levels in PBMC culture supernatants were measured using the MILLIPLEX MAP Human Cytokine/Chemokine Magnetic Bead Panel-Custom 6 Plex-Immunology Multiplex Assay (Millipore; #HCYTOMAG-60K). Analytes included were the following ...
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bioRxiv - Microbiology 2023Quote: Parasites were cultured in human red blood cells (Australian Red Cross Blood Bank) at 4% hematocrit in RPMI media (Sigma-Aldrich, UK), supplemented with 0.2% NaHCO3 (Thermo Scientific ...
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bioRxiv - Biochemistry 2023Quote: Human peripheral blood mononuclear cells (PBMC) were isolated by a density gradient centrifugation method using Histopaque-1077 (Sigma-Aldrich, cat. no. 10771) from venous blood anticoagulated with EDTA (Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... Glucose was restored to 5mM to mimic the human blood glucose level by diluting from a 500mM stock of glucose (Sigma-Aldrich, G8270). β-Hydroxybutyrate (Cayman Chemical ...
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bioRxiv - Biochemistry 2023Quote: Human thyroid cancer B-CPAP (ACC 273, DSMZ-German Collection of Microorganisms and Cell Cultures GmbH) and 8505C cells (Sigma No. 94090184) were cultured in the standard conditions in RPMI 1640 and DMEM/F12 HAM ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... CFBE-F508del-CFTR or CFBE-WT-CFTR were seeded at 140.000 cells/well in a FNC-coated 24-well plate for a day and then incubated for 24 h with ascending concentrations of correctors diluted in growth medium deprived of FBS but supplemented with 1% Human Serum Albumin (HSA, Sigma-Aldrich, #A1653). The same protocol was used for treatment with proteasome inhibitor MG-132 (Sigma-Aldrich) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... After co-incubation with correctors for 24 h in medium (MucilAir™ medium, Epithelix) supplemented with 60 % human serum (#4522, Sigma-Aldrich) at 37°C ...
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bioRxiv - Genetics 2023Quote: ... HSPCs were transferred into erythroid differentiation medium (EDM) consisting of IMDM (GibcoTM, 12440061) supplemented with 330 μg/ml holo-human transferrin (Sigma, T0665-1G), 10 μg/ml recombinant human insulin (Sigma ...
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bioRxiv - Molecular Biology 2023Quote: Hek293T cells (human embryonic kidney cells obtained from Petr Svoboda, IMG of Czech Academy of Sciences) were cultured in DMEM (Sigma, cat # D6429) supplemented with 10% fetal bovine serum FBS (Sigma ...
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bioRxiv - Biochemistry 2023Quote: Human osteosarcoma SJSA-1 cells were purchased from ATCC (Manassas, VA, USA) and cultured with RPMI 1640 medium (Sigma Aldrich, Merck, Germany), supplemented with 10% fetal bovine serum (FBS) ...
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bioRxiv - Biochemistry 2022Quote: ... and hearts), mouse organs (livers, brains, and hearts) and human hearts and brains was performed with a commercial kit (Sigma-Aldrich, 11814770001) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... stably expressing human ACE2 and TMPRSS2 (ref.42, 43) were maintained in Dulbecco’s modified Eagle’s medium (high glucose) (Sigma-Aldrich, Cat# 6429-500ML) containing 10% fetal bovine serum and 1% penicillin-streptomycin (Sigma-Aldrich ...
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bioRxiv - Immunology 2023Quote: ... followed by the addition of anti-human horseradish peroxidase (HRP)-conjugated secondary antibodies for IgG detection (diluted 1:50000, Sigma-Aldrich, A0170) to each well for 30 minutes ...
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bioRxiv - Genetics 2024Quote: ... Hydrocortisone: 0.18 µg/ml and Human epidermal growth factor: 0.2 ng/ml with added CaCl2 (340 µM, Sigma-Aldrich, Saint Louis, USA) and 1% penicillin/streptomycin ...
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bioRxiv - Cancer Biology 2024Quote: ... The primary antibody solution was prepared with a dilution of 1:100 of both rabbit anti-human anti-ZO-1 (Sigma-Aldrich, AB2272) and mouse anti-human anti-Vimentin (Sigma-Aldrich ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... HEK 293TACE2 and A549ACE2 cells were generated by a transduction with a lentivirus expressing human ACE2 (kind gift from Benjamin Murrel, Karolinska Institutet) in the presence of 6 μg/ml Polybrene (Sigma-Aldrich, H9268). Clones were isolated and selected for homogeneous ACE2 expression as determined by HTM ...
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bioRxiv - Neuroscience 2024Quote: The cytokine concentrations of organoid lysate samples were quantified on the Luminex FLEXMAP3D platform using the Milliplex human cytokine and chemokine magnetic bead panel kit (Millipore HCYTOMAG-60K), measuring a broad panel of immune signaling proteins that allow for an unbiased survey of immune cues activating a diverse set of downstream intracellular pathways ...
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bioRxiv - Biophysics 2024Quote: ... γ-cyclodextrin (γ-CD, CAS#7585-39-9), C-reactive protein (CRP, AG723) and human serum (H4522-100ML) were ordered from Sigma-Aldrich. 1 µM γ-CD stock suspension was prepared with MilliQ ...
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bioRxiv - Neuroscience 2024Quote: ... reconstructed from a clonotypic plasma blast obtained from the cerebrospinal fluid of a AQP4-IgG-positive NMOSD patient52 together with human complement (15-30 U/ml, Sigma-Aldrich, #S1764) was injected using a custom-made microinjector and a pulled glass capillary (Drummond PCR micropipettes ...
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bioRxiv - Neuroscience 2024Quote: Human brain pericytes were cultured on a glass coverslip coated with 10 mg/mL of poly-L-lysine (Millipore Sigma, Cat# P8920). Cells were fixed and permeabilized with 4% paraformaldehyde for 15 min ...
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bioRxiv - Bioengineering 2024Quote: Recombinant active human MMP-9cd (auto-cleavage resistance) 34 with an N-terminal His6x tag was expressed in Rosetta™(DE3) pLysS (Millipore Sigma) cells using a pET-28a(+)-His6x-MMP-9cd vector as previously described 33,35,36 ...
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bioRxiv - Cell Biology 2020Quote: ... cell lysate was incubated with RIP binding buffer containing magnetic beads coated with human Ago2 antibody or mouse IgG antibody (control) (Millipore, Billerica, MA, USA). Subsequently ...
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bioRxiv - Developmental Biology 2021Quote: ... mouse anti-human α-smooth muscle actin (Cy3 conjugated, cat. #C6198, Sigma Aldrich, 1:500; FITC conjugated, cat. #F3777, Sigma Aldrich, 1:500), rabbit anti-HISTONE H3 (phospho S10 ...
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bioRxiv - Microbiology 2019Quote: ... RPE-1 cells (ATCC® CRL-4000™) and Human foreskin fibroblasts (Hff1; ATCC® SCRC-1041™) were maintained in DMEM (Sigma) supplemented with 10% heat-inactivated FBS and 100 U/mL penicillin and 100 µg/mL streptomycin ...
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bioRxiv - Physiology 2019Quote: Sperm from the cauda epididymides of 2-month-old C57Bl/6N male mice were allowed to swim out in Embryomax Human Tubal Fluid medium (HTF, Millipore, MR-070-D) and incubated for capacitation ...