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Citations for Millipore Sigma :
401 - 450 of 10000+ citations for Recombinant Human FLT1 Protein His Avi tagged Biotinylated since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Western blots targeting FLAG-tagged proteins were performed using FLAG® M2 monoclonal antibody (Sigma) as described previously [24 ...
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bioRxiv - Genetics 2023Quote: ... FLAG-tagged proteins were immunopurified using preformed complexes of anti-FLAG M2 antibody (Sigma Aldrich) and protein G Sepharose (GE healthcare).
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bioRxiv - Immunology 2023Quote: ... Co-IP of FLAG-tagged proteins was performed by incubating M2 anti-FLAG resin (Sigma) with clarified cell lysates for 2 h at 4°C with end-over-end rotation ...
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bioRxiv - Biochemistry 2024Quote: ... Fractions containing pure polyhistidine-tagged proteins were pooled and concentrated by centrifugation filtration (EMD Millipore; using appropriate MWCO ...
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bioRxiv - Microbiology 2021Quote: ... Target proteins were bound to 1 mL Ni-NTA His-Bind resin (Millipore Sigma) in gravity columns ...
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bioRxiv - Biochemistry 2020Quote: ... FphF protein was initially purified by Ni2+ affinity chromatography (HIS-Select resin, Sigma-Aldrich) using an elution buffer containing 50 mM Tris pH 8.0 ...
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bioRxiv - Microbiology 2020Quote: ... Protein expression was validated by microarray using monoclonal anti-polyhistidine (clone His-1, Sigma).
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bioRxiv - Cell Biology 2020Quote: ... bound proteins were detected by immunoblotting with primary mouse anti-His (Sigma□Aldrich, Taiwan) or anti-GST primary antibodies (Sigma□Aldrich ...
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bioRxiv - Biophysics 2024Quote: ... Protein was initially purified using affinity chromatography using HIS-Select Cobalt Affinity Gel (Sigma) on a gravity flow column ...
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bioRxiv - Biochemistry 2021Quote: ... His-tagged CLIP-170 fragments and mutants were expressed in BL21 (DE3) and purified by the standard His-tagged purification protocol from Novagen (69670-5, Sigma-Aldrich) with the following modifications ...
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bioRxiv - Biochemistry 2023Quote: Thermal stability of BSA and HSA variants (His-tagged form) and of commercial wild type HSA and BSA (Sigma-Aldrich A1653 and A7638, respectively) was measured by nanoscale differential scanning fluorimetry (nanoDSF) ...
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bioRxiv - Cell Biology 2021Quote: ... Then cell lysates were subject to protein immunoprecipitations using indicated beads (For N-Flag-S-protein-tagged, FLAG M2 (Sigma-Aldrich) or S-protein beads (Millipore ...
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bioRxiv - Genomics 2020Quote: ... 3.33 ml 75 mg/ml Human Recombinant Albumin (A0237, Sigma-Aldrich, St Louis, MO, USA), 2.5 mL 1 M lactate in 1 M HEPES (L(+)Lactic acid sodium (L7022 ...
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bioRxiv - Cell Biology 2019Quote: ... growth media was replaced with media containing 50ng/ml BMP-12 (recombinant human, Sigma Aldrich) and 10ng/ml IGF-1 (recombinant human ...
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bioRxiv - Neuroscience 2021Quote: ... cells were stimulated with human TNF recombinant (10 ng/ml) (Sigma-Aldrich Corporation, Missouri, EUA) for 24 hours.
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bioRxiv - Developmental Biology 2021Quote: ... 10 μg/ml BSA fraction V and 10 μg/ml human recombinant insulin (both Sigma). Fluorescence was measured by flow cytometry 24 h after the addition of RA.
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bioRxiv - Developmental Biology 2021Quote: ... 10 μg/ml BSA fraction V and 10 μg/ml human recombinant insulin (both Sigma)) ...
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bioRxiv - Developmental Biology 2021Quote: ... Netherlands) and 48 hours later with 7.5 IU recombinant human chorionic gonadotrophin/hCG (Sigma-Aldrich, St ...
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bioRxiv - Microbiology 2022Quote: ... and 25 µg mL−1 of recombinant human purified E-cadherin (5085, Sigma-Aldrich, USA) in PBS for 1 h at 37 °C ...
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bioRxiv - Cancer Biology 2022Quote: ... and 25 ng/ml of recombinant human Epidermal Growth Factor (EGF) (Sigma, Sitnyakovo Blvd, Bulgaria). Cells were then filtered through a 100 μm filter to retain the non-digested fragments ...
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bioRxiv - Cancer Biology 2023Quote: ... in the presence or absence of 100 pg/ml recombinant human (rh)-IL21 (Sigma-Aldrich). The next day ...
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bioRxiv - Developmental Biology 2023Quote: ... treated twice every 12 h with human recombinant sDLL4 (1µg/ml, Cat#: SRP3026, Sigma Aldrich) for a total of 24h ...
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bioRxiv - Genomics 2023Quote: ... 3.33 ml 75 mg/ ml Human Recombinant Albumin (A0237, Sigma-Aldrich, St Louis, MO, USA), 2.5 mL 1 M lactate in 1 M HEPES (L(+)Lactic acid sodium (L7022 ...
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Mammalian-unique eIF4E2 maintains GSK3β proline kinase activity to resist senescence against hypoxiabioRxiv - Cell Biology 2020Quote: ... Biotinylated proteins were pulled down by streptavidin-agarose beads (50 μL beads/sample, S1638, Sigma) overnight to purify nascent proteins for western blotting analysis.
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bioRxiv - Microbiology 2022Quote: ... Biotinylated proteins were captured in ELISA plates precoated with 5 μg/ml of avidin (Sigma) in Na2CO3-NaHCO3 buffer (pH 9.6 ...
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bioRxiv - Biophysics 2021Quote: ... Plasminogen (human plasma purified protein, Sigma-Aldrich, Germany) and Fibrinogen (human plasma purified protein ...
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bioRxiv - Biophysics 2021Quote: ... and Fibrinogen (human plasma purified protein, Sigma-Aldrich). Experimental conditions are presented in the table I.
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bioRxiv - Biophysics 2021Quote: Plasminohen (human plasma purified protein, Sigma-Aldric, Germany)
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bioRxiv - Developmental Biology 2019Quote: ... 200nL 0.1µg/µL human noggin protein (Sigma H6416) was injected into the blastocoel of embryos at stage 8 ...
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bioRxiv - Systems Biology 2024Quote: ... Samples were stored at –20 °C and insulin secretion was quantified using human (Millipore Cat. # HI-14K) insulin radioimmunoassay kits.
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bioRxiv - Microbiology 2021Quote: ... Immunoprecipitation of 3×Flag-tagged proteins was performed using α-FLAG M2 affinity gel (Sigma-Aldrich) following the manufacturer’s recommendations ...
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bioRxiv - Molecular Biology 2022Quote: ... induction of AID-tagged protein depletion was performed with 3-indole-acetic acid (IAA) (Sigma-Aldrich) during the exponential growth phase (approx ...
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bioRxiv - Neuroscience 2022Quote: ... To immunoprecipitate FLAG-tagged proteins the supernatant was incubated with anti-FLAG M2 Affinity Gel (Sigma) for 1h at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... HA-tagged proteins were detected by anti-HA mAb (clone HA-7, 1:5000, Sigma-Aldrich); NSP5 and VP2 proteins were detected using anti-NSP5 guinea pig serum (1:5000 ...
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bioRxiv - Molecular Biology 2019Quote: Purified FLAG-tagged CSB proteins were immobilized on pre-equilibrated Anti-FLAG M2 Magnetic Beads (Sigma) for 2 h at 4°C ...
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bioRxiv - Genetics 2020Quote: ... Epitope-tagged proteins were immunoprecipitated with either anti-Flag- or anti-HA-coated magnetic beads (Sigma and Pierce ...
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bioRxiv - Cell Biology 2023Quote: ... FLAG-tagged proteins were eluted from the beads with 200 µg/ml FLAG peptide (Sigma-Aldrich) in 0.5 ml of lysis buffer B ...
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bioRxiv - Plant Biology 2023Quote: ... The HAHis-tagged SbtB proteins were detected with monoclonal antibodies against the HA-epitope (Sigma, USA). Secondary ...
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bioRxiv - Genetics 2023Quote: ... Proteins tagged with 6His10FLAG were detected by immunoblot-ting using an anti-FLAG mouse antibody (Sigma ANTI-FLAG M2 antibody F3165 ...
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bioRxiv - Molecular Biology 2023Quote: ... Flag-tagged proteins were eluted from the beads using 150 ng/ml of 3xFLAG peptide (Sigma) in 50–100 ul of elution buffer (20 mM HEPES-KOH (pH 7.5) ...
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bioRxiv - Cancer Biology 2024Quote: ... and equilibrated before FLAG tagged proteins were immunoprecipitated using anti-Flag M2 agarose beads (Sigma #A2220).
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bioRxiv - Immunology 2020Quote: ... For IgM antibody detection biotinylated anti-human IgM (affinity purified, μ-chain specific, Sigma-Aldrich cat. Nr. B1140) was used as secondary antibody.
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bioRxiv - Immunology 2020Quote: ... For IgM antibody detection biotinylated anti-human IgM (affinity purified, μ-chain specific, Sigma-Aldrich cat. Nr. B1140) was used as a secondary antibody ...
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bioRxiv - Genetics 2019Quote: Recombinant proteins were expressed in Escherichia coli BL21(DE3)pLys using pET28a+ (Novagen) and purified using Ni-Sepharose affinity medium (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... The recombinant proteins were produced and purified on glutathione-agarose column (G4510, Sigma) for GST-tagged protein or on TALON-Cobalt beads column (635507 ...
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bioRxiv - Plant Biology 2023Quote: ... The over-expressed recombinant proteins were then coupled to CNBr-activated Sepharose (Sigma) at a ratio of 2.5 mg protein to 1 ml Sepharose slurry as described by the manufacturers.
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bioRxiv - Biophysics 2023Quote: ... recombinant Rad26 protein was expressed in Escherichia coli strain Rosetta 2(DE3) (Novagen) and purified by Ni-NTA agarose (Qiagen) ...
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bioRxiv - Genetics 2023Quote: ... and samples were analyzed by standard immunoblotting procedures using 1:3000 peroxydase-conjugated antiperoxydase (PAP, to detect protein-A- tagged proteins) (Sigma, #P1291, RRID:AB_1079562), 1:3000 monoclonal anti-FLAG antibody (Sigma ...
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bioRxiv - Immunology 2022Quote: ... coli and protein was isolated and purified on His-binding nickel resin (Novagen, Madison, Wisconsin). Nitrophenyl-haptenated ovalbumin (NP-OVA ...
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bioRxiv - Biophysics 2021Quote: The human esaHis-tagged PTPN11 (residues 1-528) cDNA was cloned in a pET-26b vector (Novagen, MA, USA). Nucleotide substitutions associated with NS or leukemia were introduced by site-directed mutagenesis (QuikChange site-directed mutagenesis kit ...