Labshake search
Citations for Millipore Sigma :
351 - 400 of 4062 citations for Recombinant Human ACP1 GST tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biochemistry 2020Quote: ... 100 μl of monoclonal mouse-anti-GST antibodies (Sigma-Aldrich), monoclonal anti-His6 antibodies (ThermoFisher ...
-
bioRxiv - Biochemistry 2022Quote: ... GST tag was then cleaved off using preScission protease (Sigma) overnight at 4°C ...
-
bioRxiv - Plant Biology 2021Quote: ... and analyzed by immunoblotting using either anti-GST antibody (Sigma) or anti-MBP antibody (NEB).
-
bioRxiv - Biochemistry 2021Quote: ... Soluble lysates were incubated with GST-Bind resin (EMD Millipore) for 30 min at 4 °C ...
-
bioRxiv - Genetics 2020Quote: ... H9 cells were cultured with 6μM Chir99021 (GST inhibitor [Millipore]) in B27-medium (RPMI 1640 medium [Invitrogen] supplemented with 1× B27 minus insulin [50× ...
-
bioRxiv - Biochemistry 2022Quote: ... GST proteins were eluted with 10 mM reduced glutathione (Sigma) in 50 mM Tris pH 8.0.
-
bioRxiv - Cell Biology 2022Quote: ... Anti-GST-HRP (RPN1236, 1:10000) was from Sigma Aldrich. Anti-phosphotyrosine antibody (4G10 ...
-
bioRxiv - Genetics 2022Quote: ... and detected with His and GST antibodies (Sigma-Aldrich, USA), respectively.
-
bioRxiv - Neuroscience 2022Quote: ... followed by incubation with anti-GST mAb (1:2000, Sigma) and anti-mouse IgG-HRP (1:2000 ...
-
bioRxiv - Plant Biology 2023Quote: ... GST was removed with the Thrombin CleanCleaveTM Kit (Sigma-Aldrich) following the manufacturer instructions ...
-
bioRxiv - Cancer Biology 2024Quote: ... GST protein was purified using glutathione Sepharose 4B beads (Sigma) by rotating overnight at 4°C ...
-
bioRxiv - Biochemistry 2023Quote: ... GST-Erk2 at 20 μg/mL (Sigma-Aldrich, ref. E1283), GST-Cdk1/CyclinA2 at 20 μg/mL (Sigma-Aldrich ...
-
bioRxiv - Neuroscience 2023Quote: ... anti-rabbit TRITC tagged (1:200, Sigma) and anti-mouse FITC tagged (1:200 ...
-
bioRxiv - Neuroscience 2021Quote: ... Fc-tagged and non-Fc-tagged proteins were concentrated using Amicon Ultra 10 kDa MWCO centrifugal filter units (Millipore), dialyzed against PBS ...
-
bioRxiv - Neuroscience 2024Quote: ... Nuclei from tagged cells were separated from non-tagged nuclei by incubation with anti-GFP (Sigma; cat#SAB4301138-100UL) and anti-myc (Sigma ...
-
bioRxiv - Cancer Biology 2022Quote: ... The chopped and digested pieces were placed in a serrated 60mm petri dishes and supplemented with 10ng/μl each of human recombinant epidermal growth factor (hEGF, Cat # E9644, Sigma-Aldrich), N2 suppliment-1X (Cat # 17502048 ...
-
bioRxiv - Microbiology 2019Quote: ... we performed growth curves in YPD liquid medium containing different concentrations of Gal-3 (Gal-3 human recombinant, expressed in E. coli, Sigma-Aldrich) in a 96-well plate (Costar ...
-
bioRxiv - Genomics 2020Quote: ... RPMI-1640 medium used to culture the MCF7 cells was additionally supplemented with 0.01 mg/ml human recombinant insulin (Sigma Aldrich, #I3536).
-
bioRxiv - Cell Biology 2021Quote: ... 250 µl of maintenance medium (50% Wnt3a-conditioned medium (ATCC#CRL-2647, Manassas, VA, USA) and 50% of 2× basal medium supplemented with recombinant human EGF (Sigma-Aldrich), recombinant human noggin (R&D Systems ...
-
bioRxiv - Molecular Biology 2022Quote: ... the native or reconstituted chromatin samples (140 μg DNA/ml) in HNE were treated with 25 μg/ml human recombinant PAD4 enzyme (Sigma Aldrich cat # SRP0329 or Cayman Chemical cat ...
-
bioRxiv - Cell Biology 2023Quote: ... 100 µM carnitine) with or without supplementation of 100 ng/ml (13.16 nmol/l) IGF1 (human recombinant IGF1, I3769, Sigma-Aldrich, Germany). Medium was changed three times per week and 48 hours before harvest ...
-
bioRxiv - Neuroscience 2023Quote: ... Recombinant Human Protein #PHG0264), 10 ng/ml epithelial growth factor (EGF, Recombinant Human Protein #PHG0311) and 10 µM ROCK Inhibitor Y-27632 (Sigma, #Y0503) were added to the media freshly before use ...
-
bioRxiv - Cell Biology 2024Quote: In vitro kinase assays were performed in the presence or absence of different recombinant human atypical PKC (50 or 100 ng of PKC ζ, #14-525 Merk Millipore; 1µg of PKC I/αPar6 complex ...
-
bioRxiv - Physiology 2024Quote: ... and 120 min after intraperitoneal injection of 1g/kg glucose (glucose tolerance test) or 0.5 U/kg of recombinant human insulin (Sigma Cat.# I6634) (insulin tolerance test).
-
bioRxiv - Microbiology 2021Quote: ... recombinant hyaluronidase (Sigma, H3506) was treated at 10 μg/mL ...
-
bioRxiv - Microbiology 2021Quote: ... recombinant neuraminidase (Sigma, N2876) was treated at 1 U/mL ...
-
bioRxiv - Immunology 2022Quote: ... recombinant CD4 (Sigma Aldrich) were added to 20 μl of KSRB with 1mM APMA ...
-
bioRxiv - Neuroscience 2019Quote: ... Cells were then incubated with anti-GST (1:800, Sigma G1160) diluted in blocking buffer for 1 hour ...
-
bioRxiv - Biochemistry 2019Quote: Glutathione S-Transferase (GST) Assay Kit (SIGMA, Saint Louis, MO, USA) was used to quantify the GST activity of samples ...
-
bioRxiv - Plant Biology 2021Quote: ... and those with GST tag was purified by glutathione sepharose (Novagen).
-
bioRxiv - Cell Biology 2022Quote: ... The GST tag was cleaved with thrombin (commercial thrombin, Sigma Aldrich), at 200 units per mL for 2h at room temperature ...
-
bioRxiv - Cell Biology 2021Quote: ... protein was purified by incubating with glutathione agarose (GST; Sigma Aldrich) or amylose resin (MBP ...
-
bioRxiv - Cell Biology 2021Quote: ... GST-VAPB was expressed in BL21 Rosetta (DE3) cells (EMD Millipore) induced with 1 mM IPTG for 4 h ...
-
bioRxiv - Biochemistry 2023Quote: ... Protease was removed by incubation with GST-Bind beads (EMD Millipore). Sid4 was concentrated using Amicon Ultra centrifugal filters (Millipore) ...
-
bioRxiv - Cancer Biology 2019Quote: HeLa was transfected with Flag-tagged Vav1 and V5-tagged VEGFR1 grown on coverslips were stained with antibody against Flag-tag (Sigma), and against V5-tag (Cell Signaling) ...
-
bioRxiv - Developmental Biology 2020Quote: ... HA-tagged or Myc-tagged proteins were isolated with anti-HA agarose or anti-c-Myc Agarose Affinity Gel (Sigma), respectively ...
-
bioRxiv - Biochemistry 2022Quote: ... PTP-tagged or MHTAP-tagged protein variants were detected using the peroxidase-anti-peroxidase soluble complex (PAP) (1:2000, Sigma) as previously described.10 Detection of 6x His-tagged recombinant POLIB variants was performed with primary antibody Penta•His (1:1000 ...
-
bioRxiv - Neuroscience 2021Quote: ... the proteins were tagged with FITC (Sigma, F7250) before encapsulation.
-
bioRxiv - Cancer Biology 2022Quote: ... HRP-tagged anti-goat secondary antibody (Sigma-Aldrich), GFP ...
-
bioRxiv - Neuroscience 2023Quote: ... and anti-mouse FITC tagged (1:200, Sigma) at room temperature in dark for 90 min ...
-
bioRxiv - Developmental Biology 2020Quote: ... individual components can be added to 500 ml media bottle at the indicated final concentrations: 1) Recombinant Human Insulin (Sigma I-1882) – 12.5 µg/ml final concentration ...
-
bioRxiv - Cell Biology 2021Quote: ... The organoids were then allowed to grow for 5 days followed by 24 hour treatment with recombinant human protein TNF-α (H8916, Sigma Aldrich) at 40ng/ml or IFN-gamma (PHC4031 ...
-
bioRxiv - Microbiology 2020Quote: ... 0.1 ng/ml human recombinant epithelial growth factor and 50 U/ml penicillin and 50 U/ml streptomycin (Sigma-Aldrich, MO, USA). The VK2 cells were seeded into 24-well tissue culture plates ...
-
bioRxiv - Developmental Biology 2022Quote: ... containing the same supplements) freshly supplemented with 25 ng/mL of human recombinant FGF4 (prepared in house) and 1 μg/mL heparin (Sigma-Aldrich, USA). TSCs were passaged every 3-5 days ...
-
bioRxiv - Molecular Biology 2024Quote: ... insulin-like growth factor 1 (IGF-1; 100 ng/ml; recombinant Human LONG R3 IGF-1, Sigma-Aldrich, Cat# I1271, MO, USA), rapamycin (10 ng/ml ...
-
bioRxiv - Immunology 2024Quote: ... MASP-3 and MAP-1 was measured in an ELISA assay where 10 µg/ml recombinant human albumin (rHA) (Sigma-Aldrich, A9731) was coated on Nunc MaxiSorp flat-bottom 96-well plates (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2024Quote: ... The coating of the Ibidi® µ-slides IV 0,4 Uncoated (Cat# 80601) with fibrin was performed by adding PBS mixed with recombinant human albumin (Sigma-Aldrich, Cat# A9731), fibrinogen from human plasma AF488 Conjugate (Thermo Fisher Scientific ...
-
bioRxiv - Microbiology 2023Quote: BCBL-1/Cas9 cells containing pLenti SpBsmBI Guide Hygro (AAVS1 or TRAIL-R1 #1/2) were treated with the indicated serial dilutions of recombinant human TRAIL (Sigma-Aldrich, T9701) and absolute cell numbers were measured 24 hours later by flow cytometry as described above for cumulative growth curves.
-
bioRxiv - Microbiology 2020Quote: ... HTF-tagged LsaA and FLAG3-tagged VgaALC proteins were detected using anti-Flag M2 primary (Sigma-Aldrich, F1804; 1:10,000 dilution) antibodies combined with anti-mouse-HRP secondary (Rockland ...
-
bioRxiv - Neuroscience 2024Quote: ... Cells co-expressing flag-tagged LRRK2 and mCherry-tagged PAK6 were stained sequentially with chicken anti-mCherry (Sigma, #AB356481, 1:1000) followed by Alexa405-coupled goat anti-chicken (Abcam ...