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Citations for Millipore Sigma :
351 - 400 of 846 citations for Ebola Virus Envelope Glycoprotein GP1 Zaire Kikwit 95 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... and resuspending in virus + polybrene (final conc 8 ug/mL, Sigma-Aldrich, TR-1003) or RPMI media + polybrene for the uninfected control at a concentration of 1E6 cells/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... the virus-containing supernatants from HEK293 cultures mixed with polybrene (Sigma, St. Louis, MO) at a final concentration of 8 mg mL-1 were added to the cells in each well ...
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bioRxiv - Bioengineering 2023Quote: ... we filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1.5mL aliquots in cryovials at -80°C.
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bioRxiv - Cancer Biology 2023Quote: ... Virus (400 µL) was added together with 5 ug/mL of polybrene (Sigma-Aldrich) and cells were centrifuged at 2,000 rpm for 2 h at 30°C ...
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bioRxiv - Synthetic Biology 2019Quote: ... 0.15 M NaCl and 2 mM EDTA pH 8 and subsequently eluted with 95% formamide (Sigma, F9037) in water ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: DINCH (CAS No. 166412-78-8; molecular formula: C6H10(CO2C9H19)2) was purchased from Sigma (purity ≥95 %). The physical chemical properties of DINCH are as follows ...
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bioRxiv - Cell Biology 2022Quote: ... Mobile phase A was (95:5 v/v) water: acetonitrile with 20 mM ammonium acetate (Sigma-Aldrich) and 20mM ammonium hydroxide (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... and boiled at 95°C for 10 min before electrophoresis on 4-12% pre-cast gels (Sigma) following manufacture instructions ...
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bioRxiv - Systems Biology 2020Quote: ... Food samples were collected in a tube prefilled with 1 ml 95% ethanol (Ethyl alcohol (Sigma-Aldrich) and Invitrogen UltraPureTM Distilled Water) ...
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bioRxiv - Cell Biology 2021Quote: ... Isolated islets were maintained (37°C, 95/5% air/CO2) in RPMI-1640 culture medium (Sigma-Aldrich), 10.7 mM glucose ...
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bioRxiv - Genetics 2020Quote: ... the cells were exposed for 24h to freshly prepared 0.1 μM rotenone (Sigma, R8875, purity HPLC ≥ 95%) diluted in neuronal maturation medium12 ...
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bioRxiv - Animal Behavior and Cognition 2024Quote: ... sections were incubated in 4°C overnight with primary antibodies directed against PSD-95 (Millipore, MAB 1598). Next ...
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bioRxiv - Microbiology 2023Quote: 0.25 mg of rhamnolipid methyl ester A (RLME A) or Rhamnolipids (95%, Di-Rhamnolipid dominant, Sigma-Aldrich) were aliquoted into 2-mL scintillation vials ...
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bioRxiv - Bioengineering 2023Quote: ... and samples were mixed 1:1 with a denaturing dye containing 95% formamide (Sigma, cat# F9037-100ML), 0.02% sodium dodecyl sulfate (SDS ...
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bioRxiv - Biophysics 2023Quote: ... with flow rate of 2.5 ml/min starting at 95% RP-HPLC solvent A (99.9% H20, 0.1% trifluoroacetic acid (TFA)(Sigma) and 5% RP-HPLC solvent B (99.9% acetonitrile ...
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bioRxiv - Cell Biology 2023Quote: ... Elution of DNA from the beads was performed by incubation with 95% formamide (Sigma Aldrich, F9037-100ML), 10 mM EDTA (Applichem ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were heated for 10 minutes at 95° C with 50 mM DL-dithiothreitol (Sigma Aldrich, 43819) and Laemmli buffer at 1X (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were heated for 10 minutes at 95° C with 50 mM DL-dithiothreitol (Sigma Aldrich, 43819) and Laemmli buffer at 1X (Bio-Rad ...
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bioRxiv - Microbiology 2021Quote: ... The filtered virus was mixed with fresh media (30% vol/vol) that included polybrene (Sigma) at a 6 μg/ml final concentration ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 µL of virus was added per well with 10 µg/mL of polybrene (Sigma) in 100 µL of EMEM without antibiotics or serum overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... Primer extension was conducted with 4 units of Avian Myeloblastosis Virus (AMV) reverse transcriptase (Sigma) for 1 hour at 15°C ...
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bioRxiv - Cancer Biology 2019Quote: ... concentrated virus was added to the cells along with 8 ng/mL polybrene (Sigma-Aldrich) and the cells were incubated at 37°C plus 5% CO2 in humidified air ...
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bioRxiv - Cancer Biology 2021Quote: ... virus was washed out and replaced with fresh complete medium with 10μg/ml blasticidin (Sigma). After 3 days ...
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bioRxiv - Cell Biology 2021Quote: ... 0.5 mL of freshly thawed virus soup was mixed with polybrene (Cat# H9268, Sigma-Aldrich) to a final concentration of 8 μg/mL and used to infect A549N and HT-1080N cell lines ...
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bioRxiv - Microbiology 2021Quote: ... Virus lytic replication was induced via addition of 2 µg/mL doxycycline hyclate (Sigma-Aldrich). All cells were cultured at 37 °C at 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: Virus titres were quantified by plaque assay in 12-well plates (Millipore Sigma, Cat# Z707775). For the quantification of FCV ...
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bioRxiv - Neuroscience 2019Quote: ... The virus was subsequently concentrated by mixing the filtered supernatant with 50% PEG-8000 (Sigma) in a ratio of 4:1 and placed at 4°C over night ...
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bioRxiv - Immunology 2020Quote: ... sucrose gradient-purified AD169r-GFP virus was transferred to a 100,000 kDa Amicon filter (Millipore), then buffer exchanged with 1× PBS ...
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Paracrine signalling between intestinal epithelial and tumour cells induces a regenerative programmebioRxiv - Cancer Biology 2021Quote: ... The virus particles were then concentrated to 300µl using Amicon Ultra Centrifugal Filters (UFC910024 SIGMA) by spinning at 1000g at 4°C for 1h ...
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bioRxiv - Cancer Biology 2021Quote: ... harvested virus was added onto target cells in the presence of 8μg/ml polybrene (Millipore). For in vivo experiments ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were infected with virus-containing media using Polybrene reagent (TR-1003-G; EMD Millipore) according to the manufacturer’s instructions and selected for 48 hr in 2 μg/mL of puromycin.
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bioRxiv - Immunology 2020Quote: ... We generated HAdV-lactoferrin complexes by incubating the virus with 40 μg lactoferrin (Sigma-Aldrich) for 30 min at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... Unabsorbed virus was removed and 2 ml of culture medium supplemented with 1% methylcellulose (Sigma) were added to each well ...
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bioRxiv - Cell Biology 2022Quote: ... Virus-containing supernatant was concentrated using Amicon Ultra-15 100kDa MWCO centrifugal filters (Millipore, UFC910024). For siRNA knockdown experiments ...
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bioRxiv - Cell Biology 2022Quote: ... Undiluted virus was added dropwise to cells with 10 μg/mL polybrene (Sigma-Aldrich; TR1003G), and incubated at 37°C for 24 hrs ...
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bioRxiv - Cell Biology 2024Quote: ... the virus-containing supernatant was filtered through a 0.45-μm pore size PVDF filter (Millipore), and lentiviral particles were concentrated using the Lenti-X Concentrator (Takara ...
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bioRxiv - Microbiology 2023Quote: ... the virus inoculum was removed and phenol-red free DMEM supplemented with .3% BSA (Sigma), 100U/ml pen/strep (Life Technologies) ...
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bioRxiv - Microbiology 2023Quote: ... virus stocks were concentrated on a 100 KDa cellulose membrane (Amicon Ultra-15, Merck Millipore) and rinsed three times successively with PBS ...
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bioRxiv - Microbiology 2023Quote: ... the virus inoculum was removed and phenol-red free MEM supplemented with 3% BSA (Sigma), 100U/mL of penicillin and 100ug/mL of streptomycin mixture (Life Technologies) ...
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bioRxiv - Cancer Biology 2023Quote: ... virus at the MOI of 50 was applied with 8 μg/ml Polybrene (Merck Millipore). Virus-containing medium was replaced with medium containing puromycin (3.5 μg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... virus-containing cell supernatants were concentrated using Amicon Ultra-15 Centrifugal Filter Units (Merck Millipore) and exchanged into TexMACS media (Miltenyi Biotec).
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bioRxiv - Cell Biology 2024Quote: ... 200 μL of the virus-containing media and 8 μg/mL polybrene (Sigma-Aldrich, H9268) was added to the cells ...
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bioRxiv - Cell Biology 2024Quote: ... the cells were infected with 300 μl of virus and 10 μg/ml polybrene (Millipore). After a 48-hr infection ...
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bioRxiv - Neuroscience 2019Quote: ... and retina were dissected under dim red light in carbogenated (95% O2-5%CO2) Ame’s medium (Sigma, A1420). Retinas were then sliced in half and incubated at 25°C in Ame’s solution for at least 30min ...
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bioRxiv - Neuroscience 2020Quote: ... slides were dehydrated for 4 min in increasing percentages of ethanol (50, 75, and 95%; all Sigma-Aldrich) and washed four times (4 min ...
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bioRxiv - Genomics 2021Quote: ... 1.5 mM MgCl2 for 30 min at 37 C followed by enzyme inactivation for 10 min at 95 C and DNA extraction using phenol:chloroform:isoamyl alcohol (Sigma). All gDNA from day 2 and 10 μg gDNA corresponding to 3 million haploid cells from day 8 was amplified in PCR reactions with maximum of 2.5 μg of DNA per reaction using NEBNext High Fidelity master mix (New England Biolabs ...
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bioRxiv - Neuroscience 2021Quote: ... Conditioning was performed on groups of around 40-50 flies with 3-octanol (around 95 % purity; Sigma-Aldrich) and 4-methylcyclohexanol (99 % purity ...
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bioRxiv - Cell Biology 2022Quote: ... and 3-Amino-1,2,4-triazole (3-AT) (≥95% TLC) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Z-VAD-FMK ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were incubated with the following primary antibodies: anti-PSD-95 clone 6G6-1C9 (1:1000, MAB1596, Millipore), anti-phospho-p44/42 MAPK (Erk1/2 ...
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bioRxiv - Cell Biology 2021Quote: ... Peptides were separated using a 75 mm × 250 μm inner diameter 1.7 μM CSH C18 analytical column (Waters) with a gradient from 95% A (0.1% formic acid, FA, Sigma) and 5% B (0.1% FA in ACN ...