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Citations for Millipore Sigma :
3251 - 3300 of 10000+ citations for SARS CoV 2 Nucleoprotein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... a 0.1% 2-Hydroxy-2-methylpropiophenone photo-initiator (PI) (Irgacure, Sigma Aldrich®) was prepared using PBS ...
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bioRxiv - Microbiology 2022Quote: ... 10 mM HEPES (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid, Sigma-Aldrich), 1.2 mg/ml Sodium Bicarbonate (Sigma-Aldrich/MilliporeSigma ...
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bioRxiv - Cell Biology 2023Quote: A 100 mM stock of 2-bromopalmitic acid (2-BP, Sigma-Aldrich 248422) or methyl-palmitate (MP ...
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bioRxiv - Immunology 2023Quote: ... for 72 h with or without 1 mM 2-desoxyglucose (2-DG, Sigma). Tumor size was assessed every day using a caliper ...
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THE OLFACTORY RECEPTOR Olfr78 REGULATES DIFFERENTIATION OF ENTEROCHROMAFFIN CELLS IN THE MOUSE COLONbioRxiv - Cell Biology 2023Quote: ... 8 ml of binding buffer [PBS-2 mM EDTA-2% BSA (Sigma Aldrich) (w/v)] were added and the mix was passed through a 40 µm filter (Avantor) ...
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bioRxiv - Cell Biology 2023Quote: – 5-iodo-2’-deoxyuridine (IdU) and 5-Chloro-2′-deoxyuridine (CldU) (Sigma-Aldrich) were dissolved in sterile DMEM at 2.5mM and 200mM respectively and stored at –20°C ...
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bioRxiv - Microbiology 2024Quote: ... and either 2 mM glutamine (Quality Biological) or 2 mM asparagine (Sigma-Aldrich) as specified for 8 h at the indicated MOI ...
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bioRxiv - Cancer Biology 2024Quote: ... 1% of a photo-initiator (2-hydroxy)2-methylpropiophenone (MPP - Sigma-Aldrich #405655), 0.5% ammonium persulfate (APS ...
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bioRxiv - Neuroscience 2024Quote: ... and 50 mM 2-Deoxy-D-glucose (2-DG, D6134-5G, Sigma-Aldrich). Changes in extracellular acidification rate (ECAR ...
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bioRxiv - Neuroscience 2022Quote: ... was carbon filtered and passed through PFA vials (Savillex 200-30-12) containing odor (2% methyl valerate in mineral oil, Sigma Aldrich product #1489977; 2% 2-heptanone in mineral oil, Sigma Aldrich product # 537683 ...
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Characterization of pyruvate dehydrogenase complex E1 alpha and beta subunits of Mycoplasma synoviaebioRxiv - Microbiology 2019Quote: ... Recombinant rMSPDHA and rMSPDHB were then expressed through 1.0 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) induction and purified using Ni-NTA His-Bind® Resin Kit (Novagen, San Diego, USA). The purified protein products were quantified by using BCA Protein Assay Kit (Thermo Scientific-Pierce ...
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bioRxiv - Biochemistry 2021Quote: ... His-tagged CLIP-170 fragments and mutants were expressed in BL21 (DE3) and purified by the standard His-tagged purification protocol from Novagen (69670-5, Sigma-Aldrich) with the following modifications ...
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bioRxiv - Molecular Biology 2019Quote: ... and zinc binding proteins were eluted according to the supplied protocol using HIS-Select wash and elution buffers purchased from Sigma-Aldrich (Saint Louis, MO).
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bioRxiv - Genomics 2022Quote: ... or T-175 flasks (Hi-C, CUT&RUN) at a density of 1 × 105 cells/mL and treated with 25 nM PMA (Sigma-Aldrich, cat # P1585-1MG). After 24h ...
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bioRxiv - Biochemistry 2023Quote: Thermal stability of BSA and HSA variants (His-tagged form) and of commercial wild type HSA and BSA (Sigma-Aldrich A1653 and A7638, respectively) was measured by nanoscale differential scanning fluorimetry (nanoDSF) ...
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bioRxiv - Immunology 2019Quote: ... immature BMDCs were harvested and re-plated in BMDC media supplemented with 200 ng/ml LPS (Escherichia coli 026:B6; Sigma-Aldrich) for at least 24h ...
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bioRxiv - Biochemistry 2020Quote: ... coli BL21 Star™ (DE3) cells were cultured in LB broth supplemented with 100 μg/ml ampicillin (Sigma Aldrich, Darmstadt Germany) to select for pASKIBA3 and 50 μg/ml spectinomycin (Sigma Aldrich ...
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bioRxiv - Neuroscience 2021Quote: ... then were subjected to either single saline or LPS (lipopolysaccharide from Escherichia coli O26:B6; 200 nl of 5 mg/ml, rate=200 nl/10 min; #L8274, Sigma-Aldrich) injections using a glass micropipette [82] ...
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bioRxiv - Microbiology 2020Quote: Wild type T7 phage (T7Select®) and host BL21 Escherichia coli bacteria strain were purchased from Millipore Sigma (Burlington, MA, US). To prepare a sufficient concentration of phage for experiments ...
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bioRxiv - Molecular Biology 2021Quote: After 16 h primary hepatocytes were stimulated with a mix of 100 ng mL−1 LPS (from Escherichia Coli O111:B4, #L2630, Sigma Aldrich), and species autologous cytokines ...
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bioRxiv - Microbiology 2019Quote: Aiptasia larvae were washed and diluted to a concentration of 300-500 larvae/ml and were then incubated with 20 µg/µl LPS (lipopolysaccharides from Escherichia coli O127:B8, Sigma-Aldrich), 1 µm XMD17-109 (0.1% DMSO [Dimethylsulfoxid ...
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bioRxiv - Immunology 2022Quote: ... 2 × 105 MOs were stimulated for 24 h at 37 °C and 5% CO2 with 1 μg/mL LPS (Escherichia coli 0127:B8, Sigma-Aldrich). After LPS elimination ...
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bioRxiv - Immunology 2022Quote: ... The final cell culture concentrations of the used stimuli were as follows: 10 ng/mL Escherichia coli LPS (serotype 055:B5, Sigma-Aldrich), 10 µg/mL Pam-3-Cys (EMC micro- collection) ...
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bioRxiv - Biochemistry 2022Quote: ... coli cells expressing either WT- or E14Q-EmrE constructs were grown in Mueller-Hinton broth (Sigma, 100µg/mL carbenicillin, pH 7.0) from a single colony to an OD of 0.2 at 37 °C ...
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bioRxiv - Molecular Biology 2022Quote: ... Isotopically labelled proteins were expressed using Escherichia coli BL21 DE3 cells in M9 minimal media supplemented with 15NH4Cl (Sigma-Aldrich ISOTEC). Chaetomium thermophilum Naa5082-289 containing a C-terminal His-tag was expressed using E ...
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bioRxiv - Pathology 2020Quote: ... (from Streptomyces verticillus, Cat. No. B8416-15U) and lipopolysaccharide (LPS, from Escherichia coli, Cat. No. L4391) were purchased from (Sigma, Israel).
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bioRxiv - Molecular Biology 2019Quote: ... coli as a fusion protein with the ketosteroid isomerase (KSI) using the pET31 plasmid (Kuliopulos et al., 1994) (Novagen/EMD Biosciences). KSI fusion proteins are concentrated in inclusion bodies in E ...
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bioRxiv - Biochemistry 2019Quote: ... coli grown in M9 minimal medium containing of a methionine synthesis-inhibiting cocktail (100 mg of Lys, Phe, Thr (Sigma Aldrich) and 50 mg of Ile ...
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bioRxiv - Neuroscience 2020Quote: Tissue cultures (≥ 18 days in vitro) were treated with lipopolysaccharide (1 µg/ml; LPS from Escherichia coli O111:B4, Sigma #L4391) for 3 days (c.f. ...
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Stem cell-derived macrophages as a new platform for studying host-pathogen interactions in livestockbioRxiv - Immunology 2021Quote: ... Medium was replaced with fresh Macrophage Activation medium containing either Lipopolysaccharides (LPS, 200 ng/ml, from Escherichia coli O111:B4, Sigma #L4391) or Poly(I:C ...
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bioRxiv - Biochemistry 2021Quote: The pOP3MT plasmid containing the sequence of the mature form of SKD3 was transformed into Escherichia coli BL21(DE3) (Millipore-Sigma). Cells were cultured in 2 × 5 ml LB broth with 100 μ g/ml ampicillin for 16 hours at 30 °C with shaking ...
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bioRxiv - Molecular Biology 2021Quote: ... n = 3 for each group) were intraperitoneally injected with lipopolysaccharide (LPS; Escherichia coli 0111:B4, Sigma-Aldrich, St Louis, MO, USA), polyinosinic:polycytidylic acid [poly(I:C) ...
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bioRxiv - Microbiology 2021Quote: ... Mice were also inhaled aerosol of 1mg/ml LPS 5 ml (Lipopolysaccharides from Escherichia coli O55: B5, L2880, Lot#028M4094V; Sigma-Aldrich) dissolved in PBS or 0.8 mg/ml fMLP 2.5 ml (N-Formyl-Met-Leu-Phe ...
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bioRxiv - Microbiology 2021Quote: Mice were injected with a single intraperitoneal dose of 5mg/ml LPS (Lipopolysaccharides from Escherichia coli O111: B4, L2630, Lot#026M4022V; Sigma-Aldrich) dissolved in PBS at 7.5-10 mg/kg body weight and lungs were imaged after 3 ...
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bioRxiv - Neuroscience 2020Quote: A subset of male Long-Evans rats received intraperitoneal injections of 1.0 mg/kg lipopolysaccharide (LPS) from Escherichia coli (Sigma-Aldrich, L2630) in sterile saline (0.9% ...
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bioRxiv - Immunology 2022Quote: ... coli HM605 was grown by culturing a single colony in 5 millilitre (mL) Luria Bertani (LB) Broth (Cat#L3022, Sigma-Aldrich) with 50 µg/mL ampicillin (Cat#A9518 ...
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bioRxiv - Microbiology 2023Quote: Newborn mice were randomized to treatment groups with PBS control or incremental intranasal doses of Escherichia coli 055: B5 LPS (Millipore Sigma). Pups were treated on postnatal day 3 (PN3 ...
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bioRxiv - Molecular Biology 2022Quote: ... coli cells were grown at 37 °C and were later subjected to Isopropyl β-d-1-thiogalactopyranoside (IPTG, Sigma-Aldrich, USA) induction at an OD600 ∼ 0.6-0.8 with 0.7 mM at 37 °C for 6 hours for PfPHB1-pGEX-4T 1 and 0.7 mM at 16 °C for 20 hours for PfPHB2-pGEX-4T 1 ...
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bioRxiv - Neuroscience 2022Quote: ... the mice were injected intraperitoneally with 5 mg/kg as in Verdonk 2016 (Verdonk et al., 2016) of LPS from Escherichia coli (Sigma-Aldrich) dissolved in 0.9 % saline and the control group (8 animals ...
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bioRxiv - Immunology 2023Quote: ... 5 µg/mL BCG vaccine (BCG-Bulgaria, InterVax, Canada) or 1 ng/mL Escherichia coli lipopolysaccharide [13] (LPS; serotype 055:B5, Sigma-Aldrich) in RPMI with 10% human pooled serum ...
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bioRxiv - Immunology 2023Quote: We incubated four AWFs and four CP samples (100 µl) with increasing concentrations of Escherichia coli LPS (0, 50, 100, 200, and 500 μg/ml) (Sigma, USA) in 10 mM Tris for 30 min at pH 7.4 and 37°C ...
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bioRxiv - Plant Biology 2023Quote: ... Expression of the recombinant proteins was induced by 1 mM isopropyl β-D-thiogalactopyranoside at 37 °;C for 5 h in host Escherichia coli strain Rosetta (DE3) pLysS cells (Novagen). After induction ...
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bioRxiv - Molecular Biology 2023Quote: ... such as lipopolysaccharide (10 ng/mL, LPS; catalog no. L3024, from Escherichia Coli. O111:B4, Sigma-Aldrich, St. Louis, United States) or tumor necrosis factor-alpha (1 ng/mL ...
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bioRxiv - Bioengineering 2023Quote: ... 1 mg/ml PBS-LPS solution (LPS from Escherichia coli O55:B5, Product Number: L4005, CAS-No. 93572-42-0, Sigma Aldrich) was administered to all mice via intraperitoneal injection daily for two weeks ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Polarization of M0 macrophages for 48 h in macrophage medium supplemented with 100 ng/mL lipopolysaccharide (LPS; Escherichia coli O127:B8, Sigma Aldrich) and 20 ng/mL interferon-γ (Peprotech ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli cells were grown in M63 minimal medium62 using the necessary nutritional supplements and 30 mM glycerol (Sigma, St. Louis, MO) as a carbon source ...
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bioRxiv - Biochemistry 2024Quote: ... coli BL21 (DE3) cells transformed with this vector were grown in LB medium supplemented with ampicillin (50 µg/mL) (Sigma A9518) and protein expression was induced with 0.5 mM isopropyl β-D-1-thiogalactopyranoside (IPTG ...
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bioRxiv - Immunology 2024Quote: Eight-week-old WT and MCfosB- mice were were challenged with an intraperitoneal injection of 1mg/Kg of LPS from Escherichia coli O55:B5 (Sigma-Aldrich) in μL of saline ...
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bioRxiv - Molecular Biology 2022Quote: ... 3 µl of each stock was then spotted onto the surface of YPD plate (Yeast Extract 1%, Peptone 2%, Dextrose 2%, Agar 2%) without and with 1µg/mL tunicamycin (Sigma-Aldrich Inc., MI, USA). Plates were then incubated for 48-72 hours at 30°C before capturing their photographs ...
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bioRxiv - Bioengineering 2022Quote: Acetone and Irgacure 2959 (2-hydroxy-1-[4-(2-hydroxyethoxy) phenyl]-2-methyl-1- propanone were obtained from Sigma-Aldrich (St. Louis, MO). 3-([2-(Methacryloyloxy)ethyl]- dimethylammonio)propionate (CBMA ...