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Citations for Millipore Sigma :
3201 - 3250 of 10000+ citations for SARS CoV 2 Nucleoprotein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... The SMC1A or SMC3 HDs-coding plasmids were respectively co-transformed with the RAD21C- or RAD21N-coding plasmids into chemically competent Escherichia coli BL21(DE3) cells (Novagen). Co-transformed cells were selected using the appropriate antibiotics ...
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bioRxiv - Microbiology 2023Quote: ... coli RHO3 strains were used for conjugation and medium was supplemented with 400 μg/mL 2,6-Diaminopimelic acid (Sigma-Aldrich, Merck Life Science ...
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bioRxiv - Molecular Biology 2023Quote: ... coli MC1061 strain and Luria-Bertani (LB)-agar supplemented with ampicillin (100 μg/mL, Sigma-Aldrich, St. Louis, MO, USA) for selection ...
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bioRxiv - Cell Biology 2023Quote: ... The NF-κB activation inhibitor BAY 11-7085 and lipopolysaccharides (LPS) from Escherichia coli O55:B5 were from Sigma Aldrich. Recombinant human vascular endothelial growth factor-165 (VEGF ...
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bioRxiv - Biochemistry 2023Quote: ... Bacteria Escherichia coli and Bacillus subtilis strains were grown in Luria Bertani (LB) medium (Sigma, St. Louis, MO, United States) at 37°C and 180 rpm ...
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bioRxiv - Bioengineering 2023Quote: ... tissue cultures were treated with bacterial lipopolysaccharide (LPS from Escherichia coli O111:B4, #L4391, Sigma-Aldrich, UK, 1 µg/mL) for 3 days inside the incubator and imaged before and after 3 days of treatment as previously described.51
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bioRxiv - Neuroscience 2023Quote: ... Cells at passage 9 to 15 were treated with LPS (from Escherichia coli O55:B5, 100 ng/mL, Sigma #L6529) or with IFNγ (100 ng/mL ...
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bioRxiv - Biochemistry 2023Quote: ... Both constructs employed a linker with a rhinovirus 3C protease (PreScission Protease) recognition site and were transformed into Escherichia coli Rosetta2(DE3) cells (Novagen) for overexpression ...
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bioRxiv - Biochemistry 2023Quote: ... The ligated pTrcHisB/PRNP and pTrcHisB/Prnp constructs were used to transform the Escherichia coli host strain BL21(DE3) (Novagen), genotype F′ ompT hsdSB (rB-mB- ...
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bioRxiv - Immunology 2023Quote: ... Lipopolysaccharides from Escherichia coli 055: B5 (L2880) and Phorbol 12-myristate 13-acetate (PMA) (P1585) were obtained from Sigma-Aldrich. Yoda1 (5586 ...
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bioRxiv - Immunology 2024Quote: ... was diluted in pre-warmed complete RPMI to a final concentration of 0.5ug/ml and 1.0ug/mL and was applied for 4 hours prior to lipopolysaccharide (LPS, from Escherichia coli O111:B4, Sigma-Aldrich) treatment ...
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bioRxiv - Cell Biology 2024Quote: ... His-tagged PRDX6 from rat (gene cloned into pET15b NdeI and XhoI restriction sites) as produced in the Escherichia coli strain Origami (DE3) (Novagen). 20 µL of Prdx6- Pet15b transformed Origami (DE3 ...
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bioRxiv - Immunology 2024Quote: ... the cells were treated with either vehicle or 500 ng/mL of LPS from Escherichia coli O55:B5 (Sigma-Aldrich). Pellets and supernatants were collected and immediately flash frozen at one hour for pellet RNA expression and supernatant analyses ...
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bioRxiv - Cell Biology 2019Quote: ... The desired Young’s modulus (E) of the pre-mixes was adjusted by mixing pre-defined ratios of 40% (w/v) acrylamide monomer (Sigma-Aldrich, A4058) and 2% (w/v ...
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bioRxiv - Developmental Biology 2021Quote: ... cells from the ATOH1-EGFP line were gently scraped off Transwell membranes and plated on poly-D-lysine (Millipore, A-003-E) and Laminin (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... Pooled culture media containing E/S was concentrated by centrifugation using Amicon ultra centrifugal filter units (MWCO 10 kDa, Sigma-Aldrich, Denmark), and filtered prior to testing of protein content by bicinchoninic (BCA ...
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bioRxiv - Neuroscience 2020Quote: ... Dissociated hippocampal neurons were plated at a density of 120,000 cells per 18-mm-diameter coverslip that was coated with 1 mg/ml poly-D-lysine (Sigma, #A-003-E) and 2.5 μg/ml laminin (Roche ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were washed 2X with YPRaff media (1X with YPRaff and 1X with YPRaff + 50 μg/ml Pronase E (Sigma-Aldrich, 1074330001)) ...
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bioRxiv - Developmental Biology 2023Quote: H&E and tartrate-resistant acid phosphatase (TRAP) staining were performed according to the manufacturer’s protocols (Sigma, St. Louis, MO, United States) as described earlier [25] ...
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bioRxiv - Genetics 2023Quote: ... The blocks were cut into 4 μm sections and stained with hematoxylin and eosin (H&E) to visualize morphology and with potassium hexacyanoferrate(II) trihydrate (Sigma cat# P3289) to visualize ferric iron deposits and counterstained with Neutral Red to visualize cell morphology (Sigma-Aldrich cat#72210).
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bioRxiv - Molecular Biology 2023Quote: ... MV4-11 cells were attached overnight by coating the coverslips in poly-D-lysine (A003-E; Sigma Aldrich; 100µg/ml in H2O) for 1 h ...
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bioRxiv - Microbiology 2024Quote: Supernatants containing VSV(GFP)ΔG particles with S and E were layered on a cushion of 20% sucrose (Sigma-Aldrich, #S9378-1KG) in PBS and centrifuged for 90 min at 4°C and 20,817 x g ...
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Autophagy suppression in DNA damaged cells occurs through a newly identified p53-proteasome-LC3 axisbioRxiv - Cell Biology 2024Quote: ... Cellular monolayers were then incubated at 37 °C and under 5% CO2 in cell culture medium containing the lysosomal protease inhibitors E-64d (10 μg/mL final concentration; #E8640, Sigma-Aldrich, USA) and pepstatin A (10 μg/mL final concentration ...
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bioRxiv - Evolutionary Biology 2021Quote: ... After expression was verified in cell lines by Western blot using anti Flag tag antibodies (Monoclonal ANTI-FLAG® M1, Sigma Aldrich #F3040), surface expression was verified for FPR1 using Thermo Fisher FPR1 polyclonal antibody ref number PA5-33534 ...
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bioRxiv - Biochemistry 2020Quote: ... Multiple clones were isolated and successful integration of N-terminal 3xFLAG tag was validated by western blotting with anti-FLAG-HRP (Sigma, A8592, 1:10,000).
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bioRxiv - Microbiology 2021Quote: ... The pre-cleared lysates were then incubated overnight at 4°C with monoclonal rabbit Anti-HA Tag antibodies (Millipore, clone 114-2C-7) at 1:400 dilutions ...
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bioRxiv - Synthetic Biology 2021Quote: The N-terminal GST-tag was removed from CBX8 proteins by TEV protease cleavage according to manufacturer’s recommendations (Sigma-Aldrich, St. Louis, MO). Briefly ...
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bioRxiv - Microbiology 2020Quote: The sequences encoding mature VdAve1 and VnAve1 were cloned into pET-15b with an N-terminal His6 tag sequence (Novagen, Madison, WI, USA) (primer sequences ...
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bioRxiv - Biochemistry 2022Quote: ... Western blots were carried out with two different antibody setups: (i) FLAG tag was incubated with monoclonal ANTI-FLAG® M2-Peroxidase (HRP) produced in mouse (Sigma-Aldrich #A8592) diluted 1:3 000 ...
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bioRxiv - Cell Biology 2022Quote: ... Strains containing AID tag alleles were treated with final concentrations of 250 µM 3-indole acetic acid (Sigma-Aldrich, St. Louis, MO, USA) in DMSO and buffered with 50 mM potassium phosphate buffer at pH 6.2 for the 30 minutes prior to harvesting or imaging ...
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bioRxiv - Molecular Biology 2023Quote: ... The Cas9 protein contained an N-terminal FLAG-tag to confirm expression (mouse anti-3x FLAG primary antibody, 1:250 dilution, RT, 3 h, Sigma-Aldrich Cat# F1804). Cells were washed with PBS buffer ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells were then washed in PBS and placed in a humid chamber to be stained with the primary antibody anti-HA tag at a concentration of 8 μg/mL (anti-rabbit, #H6908, Sigma-Aldrich, Deisenhofen, Germany) in PBS ...
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bioRxiv - Molecular Biology 2023Quote: ... 0.1% NaN3) and incubated with primary antibody (monoclonal α-V5-Tag mouse antibody /Elabscience/ and monoclonal α-Flag M2 mouse antibody /Sigma Aldrich/) and secondary antibody (AF488-conjugated goat anti-mouse antibody /Elabscience/) ...
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bioRxiv - Immunology 2019Quote: ... 1 μM oligomycin and 100 mM 2-deoxy-D-glucose (2-DG; Sigma).
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bioRxiv - Cancer Biology 2020Quote: ... SASI_Hs01_00061211, #2; SASI_Hs01_00061214), and PDX1(#1; SASI_Hs01_00108076, #2; SASI_Hs01_00108080) were purchased from Sigma. shRNA-containing plasmids targeting human and mouse RNF125 (Human ...
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bioRxiv - Developmental Biology 2022Quote: ... 2% SDS) supplemented with protease inhibitor and phosphatase inhibitors 2 and 3 (Sigma). Samples were subjected to dounce homogenization ...
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bioRxiv - Synthetic Biology 2022Quote: ... 1 % Span80 (Fluka) surfactant and 4 % 2-Hydroxy-2-methylpropiophenone (97%, Sigma-Aldrich) photoinitiator ...
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bioRxiv - Neuroscience 2020Quote: ... BCH (Slc7a5 inhibitor 2-amino-bicyclo[2,2,1]heptane-2-carboxylic acid, Sigma-Aldrich) was stored as a 100 mM stock solution in 1N NaOH ...
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bioRxiv - Physiology 2022Quote: ... GSH in deproteinated samples was derivatized with 2-vinylpyridine (Sigma-Aldrich, #13229-2) prior to assessment ...
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bioRxiv - Evolutionary Biology 2019Quote: ... 1% (v/v) photo-initiator (2-hydroxy-2-methylpropiophenone, Sigma, Product No.: 405655) in buffer (75 mM Trizma HCl pH 7.4 ...
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bioRxiv - Biochemistry 2019Quote: 2 µM mTLR9-ECD was mixed with 2 µM ssDNA oligo (Sigma-Aldrich) in 10 mM MES pH 6 ...
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bioRxiv - Physiology 2020Quote: ... elegans day 2 adult worms were fixed with 2% paraformaldehyde (Sigma Aldrich, P6148) and 1% glutaraldehyde (Sigma Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... and incubated with 500 µL of 2% 3-aminopropyltriethoxysilane (Sigma; 919-30-2) in 95% ethanol for 5 min ...
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bioRxiv - Microbiology 2021Quote: ... 2 ml/well overlay [MEM containing 2% FBS and 0.4% Tragacanth (Sigma, Israel)] was added to each well and plates were further incubated at 37°C ...
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bioRxiv - Immunology 2021Quote: ... 2 ml/well overlay [MEM containing 2% FBS and 0.4% Tragacanth (Sigma, Israel)] were added to each well and plates were further incubated at 37°C ...
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bioRxiv - Cell Biology 2020Quote: ... Bis-2-(5-phenylacetamido-1,3,4-thiadiazol-2-yl)ethyl sulfide (BPTES) (Sigma, SML0601); an irreversible O-Carnitine palmitoyltransferase-1 (CPT1 ...
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bioRxiv - Molecular Biology 2022Quote: ... The ATP regeneration system (2 mM ATP, 2 mM phosphoenolpyruvate [PEP, Sigma-Aldrich, #P7127] ...
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bioRxiv - Bioengineering 2022Quote: ... blocked with 2% Abdil (2% bovine serum albumin; Sigma Aldrich A4503 + 0.1% Tween20) for 1 hour ...
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bioRxiv - Systems Biology 2022Quote: ... Fenofibrate (2-[4-(4-Chlorobenzoyl)phenoxy]-2-methylpropanoic acid isopropyl ester) (Sigma # F6020) was injected peritoneally (4% DMSO/PBS ...
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bioRxiv - Bioengineering 2024Quote: ... then coated with 1∼2 μg cm−2 laminin (No. L2020, Sigma-Aldrich) for more than 4 hours ...