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Citations for Millipore Sigma :
3151 - 3200 of 10000+ citations for SARS CoV 2 Nucleoprotein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... 2 mM glutamine (Sigma), and 10 μg/mL gentamicin (Thermo Fisher) ...
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bioRxiv - Microbiology 2020Quote: ... culture media with serum (DMEM, low glucose, from Sigma-Aldrich; 10% HI-FBS; 1% penicillin-streptomycin solution from Sigma-Aldrich; 1% 200mM L-glutamine from Sigma-Aldrich). All assays were performed with cells in passages 4 to 10.
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bioRxiv - Microbiology 2021Quote: ... from strain BetaCoV/Wuhan/WIV04/2019 was ordered from IDT (Coralville, IA, USA) and cloned into a HIS-SUMO expression vector (a modified pET-DUET; Novagen, Madison, WI, USA). After transformation into BL21(DE3) ...
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bioRxiv - Microbiology 2022Quote: ... Sub-confluent T175 flasks of 293T cells (human embryonic kidney cell line) were transfected with the RBD-His tagged plasmid using X-tremeGENE 9 reagent (Millipore Sigma, Cat# 6365809001). The supernatant was collected six days post-transfection and filtered through 0.22μm PES membrane filters (Nalgene) ...
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bioRxiv - Microbiology 2019Quote: ... The washing step was repeated and 200 µl of a 1:5,000 dilution of the monoclonal Anti-His antibody (Sigma-Aldrich, St. Louis, MO) in PBS + 2% BSA was added to each well ...
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bioRxiv - Cell Biology 2019Quote: ... 20mM reduced glutathione) and analyzed by SDS-PAGE followed by Coomassie staining and immunoblotting with anti-His (dilution: 1:5000; Sigma-Aldrich, Steinheim, Germany), or anti-GFP-antibodies (dilution 1:1000 ...
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bioRxiv - Cell Biology 2020Quote: ... and subjected to SDS-PAGE followed by Coomassie Blue staining and Western Blot analysis using an anti-His antibody (Sigma-Aldrich, Darmstadt, Germany) or anti-MYC antibodies (Hruby et al. ...
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bioRxiv - Biophysics 2021Quote: ... Protein production was confirmed by SDS-PAGE and subsequent Western blotting analysis of a K30 sample after 24 h using an anti-His antibody from mouse (dilution 1:3000, Sigma-Aldrich, cat.no. H1029) and secondary anti-mouse IgG-HRP antibody produced in goat (dilution 1:20,000 ...
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bioRxiv - Microbiology 2021Quote: ... Sub-confluent T175 flasks of 293T cells (human embryonic kidney cell line) were transfected with the RBD-His tagged plasmid using X-tremeGENE 9 reagent (Millipore Sigma, Cat# 6365809001). Supernatant was collected 6 days post-transfection and filtered through 0.22 μm PES membrane filters (Nalgene) ...
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bioRxiv - Microbiology 2022Quote: ... and then transferred to medium differing by the absence of histidine (his) and the presence of 5 mM 3-amino-1,2,4-triazole (3-AT; Sigma-Aldrich, Saint-Louis, MO, USA). Homologous recombination between pPC86 and the PCR product then reconstituted the expression cassette encoding the AD-fused prey protein ...
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bioRxiv - Plant Biology 2022Quote: ... approximately 100 μg His-IAA17 recombinant proteins were treated with 200 μM GSNO and then labeled with biotin-maleimide (Sigma-Aldrich, Cat #: B1267). After biotinylated proteins were precipitated with cold acetone ...
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bioRxiv - Cell Biology 2024Quote: ... was added to the cell pellet and stored at −20°C prior to being assayed for insulin using human insulin specific RIA (Millipore Cat# HI-14K).
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bioRxiv - Cell Biology 2023Quote: ... the membrane was sequentially incubated at room temperature for one hour with anti-His and conjugated anti-mouse antibodies (Sigma-Aldrich, Darmstadt, Germany) (Wu et al. ...
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bioRxiv - Genetics 2023Quote: ... The samples were collected for a range of incubation times and then examined by western blotting using an anti-His antibody (Sigma, catalogue no. SAB1305538) or anti-GFP (Roche ...
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bioRxiv - Microbiology 2021Quote: ... aeruginosa strains were routinely refreshed from frozen stocks at -80°C and maintained on YPD (1% Bacto yeast extract, 2% Bacto peptone, 2% dextrose, 2% Bacto agar) plates or Pseudomonas isolation agar (Sigma-Aldrich) for in vitro experiments and LB agar (10 g/liter Bacto tryptone ...
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bioRxiv - Immunology 2024Quote: Gonadal white adipose tissue was digested into pre-warmed 2 mg/ml BSA 2% FBS RPMI supplemented with 2 mg/ml collagenase type II (Sigma, C6885) and placed under shaking at 180 rpm for 40 min at 37°C ...
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bioRxiv - Microbiology 2023Quote: ... PBMCs were activated with interleukin-2 (IL-2) 450 UI/mL (Proleukin, Novartis pharma) and polybrene 2 µg/mL (Sigma-Aldrich) during at least 2 h before infection.
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bioRxiv - Cell Biology 2023Quote: ... 20 µL of cell culture medium were incubated at 37°C for 3 hours with 20 µL reaction mix containing sodium citrate (10 mM; pH 4.2) and 4-methylumbelliferyl-2-acetamido-2-deoxy-b-D-glucopyranoside (2 mM; #474502, Sigma-Aldrich/Merck). The reaction was stopped by 5 volumes of glycine and Na2CO3 ...
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bioRxiv - Cell Biology 2019Quote: ... coli BL21 cells and the expression of the transgene induced with 1 mM IPTG (isopropyl-β-D-thiogalatopyranoside, Sigma-Aldrich). The recombinant protein was purified with agarose beads that bind specifically to the His-tag (Ni-NTA Agarose ...
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bioRxiv - Cell Biology 2020Quote: ... The GST-His6-tagged forms of LC3B-His12 and GATE-16-His12 proteins were expressed in Escherichia coli BL21(DE3) cells (Novagen) harboring the pET-41-based vectors constructed in Lysogeny Broth (LB ...
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bioRxiv - Neuroscience 2021Quote: ... Shank3+/+ and Shank3+/- mice were intraperitoneally injected 24 hours before the test with LPS at a dose of 2mg/Kg in saline (NaCl 0.9%) (Lipopolysaccharides from Escherichia coli O26:B6, Sigma-Aldrich)
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bioRxiv - Molecular Biology 2020Quote: ... double mutant KlGal80-K5A/R6Ap (NHKlGal80-K5A/R6Ap or IHGal80-K5A/R6Ap) and KlGal1p (NHGal1p) were expressed in Escherichia coli strain Rosetta (DE3)-pLys from pET-derived (Novagen) expression vectors (pETNHG80 ...
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bioRxiv - Epidemiology 2019Quote: ... and recombinant heat labile toxin β subunit protein [15,52,53] from enterotoxigenic Escherichia coli (ETEC LT β subunit) were purchased from Sigma Chemical (St ...
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bioRxiv - Biochemistry 2019Quote: ... C-ERMAD (516-560 and 516-586) and RSK1 (696-735 and 689-735) were expressed in Escherichia coli BL21 (DE3) cells (Novagen) with TEV-cleavable N-terminal GST-tag ...
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bioRxiv - Bioengineering 2021Quote: ... coli is streaked across a co- ampicillin and chloramphenicol infused agar plate (25 g mL-1 Luria Broth (Millipore; 1.10285.5007), 15 g mL-1 agar (Millipore ...
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bioRxiv - Biochemistry 2020Quote: ... revVS and shVS were purified from cultures of transformed Escherichia coli C41(DE3) cells that were induced with the Overnight Express Autoinduction Kit System 1 (Novagen). Cells were pelleted and resuspended in Buffer A containing 10mM imidazole ...
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bioRxiv - Neuroscience 2020Quote: Mice were intraperitoneally injected with 3 doses of LPS dissolved in 0.1 mol phosphate balanced solution (PBS) (Escherichia coli O111: B4; Sigma-Aldrich) (50μg/kg bodyweight for each dose; ...
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bioRxiv - Microbiology 2022Quote: ... The University of Sheffield) and Escherichia coli BL21(DE3) with or without prior transformation with the expression vector pET22b (Novagen), were cultured on Luria Broth (with or without agar ...
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bioRxiv - Biophysics 2022Quote: ... the proteins were expressed in Escherichia coli strain BL21 Star (DE3) cells using 0.4 mM Isopropyl-β-D-thiogalactopyranoside (IPTG) (Sigma) for 9 to12 hours at 20 °C ...
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bioRxiv - Microbiology 2020Quote: ... coli BL21 (DE3) (Transgen, CD601-02) and protein expression was induced by an auto-induction system (71757-5, EMD Millipore). Upon overnight induction at 25°C ...
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bioRxiv - Plant Biology 2021Quote: The coding sequence of Hd3a and RFT1 were cloned into the pCold-GST vector42 and produced as a glutathione S-transferase (GST) fusion protein in Escherichia coli BL21 Rosetta (DE3) (Novagen). Cells were grown in LB medium or minimal medium containing 0.5 g l™1 of 15N-ammonium chloride ...
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bioRxiv - Immunology 2020Quote: ... CSR to IgG3 and IgG1 was induced in T cell independent manner by exposure to 5 μg/ml of Lipopolysaccharide (Escherichia Coli LPS, 055:B5, Sigma) or LPS+rIL-4 ...
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bioRxiv - Immunology 2022Quote: ... 20 ng/ml recombinant IL-1β (Preprotech) or 100 ng/ml lipopolysaccharide from Escherichia coli O55:B5 (LPS, Sigma-Aldrich) for 6h at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... coli RHO3 strains were used for conjugation and medium was supplemented with 400 µg/mL 2,6- Diaminopimelic acid (Sigma-Aldrich, Merck Life Science ...
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bioRxiv - Cell Biology 2019Quote: ... and His12-Arf1 proteins were expressed as the N-terminal GST-His6-tagged form in Escherichia coli BL21(DE3) cells (Novagen) harboring pET-41-based vectors in Lysogeny Broth (LB ...
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bioRxiv - Molecular Biology 2019Quote: ... while LMNB1-CT was expressed in Escherichia coli B834 (DE3) cultured in LB medium with 50 μg/mL of kanamycin (Sigma). EHMT1 (2IGQ ...
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bioRxiv - Cell Biology 2019Quote: ... The sample was then cut in half with a clean razor and each piece was placed in a 6-well plate with 10 ml of ASW and lipopolysaccharide (LPS) from Escherichia coli 0111:B4 (Sigma) at a final concentration of μg/ml (or an equal volume of sterile diH2O ...
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bioRxiv - Microbiology 2020Quote: ... supplemented with 50 mg l-1 kanamycin and 34 mg l-1 chloramphenicol was used for growth of Escherichia coli Rosetta (Novagen) to induce protein production ...
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bioRxiv - Biochemistry 2021Quote: ... wild-type (WT) or D274N were individually expressed from a pET30-TEV vector in BL21 (DE3) pLysS Escherichia coli (Novagen), with protein expression being induced with 0.4 mM IPTG for 18 h at 18 °C ...
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bioRxiv - Genomics 2022Quote: ... Fifteen individuals were intra-abdominally injected with 0.25 mg of LPS (Escherichia coli O55:B5; Sigma-Aldrich, cat. no. L2880) dissolved in 50 μl of Dulbecco’s Phosphate-Buffered Saline (DPBS ...
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bioRxiv - Biochemistry 2021Quote: The pOP3MT plasmid containing the sequence of the mature form of SKD3 was transformed into Escherichia coli BL21(DE3) (Millipore-Sigma). Cells were cultured in 2 × 5 ml LB broth with 100 μ g/ml ampicillin for 16 hours at 30 °C with shaking ...
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bioRxiv - Microbiology 2020Quote: ... coli JKe201 [28] was cultured in the presence of 100 µM of diamino pimilic acid (DAP) (Sigma Aldrich D1377-10G). Salmonella enterica serovar Typhimurium SL1344 was cultured in LB in the presence of 90 µg/ml streptomycin (Sigma-Aldrich S9137-100G) ...
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bioRxiv - Molecular Biology 2021Quote: ... and TniQ were expressed in Escherichia coli BL21(DE3) while TnsC was expressed in Rosetta™(DE3)pLysS (Novagen: #70956) containing a pLysS-tRNA plasmid ...
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bioRxiv - Physiology 2020Quote: ... tau/NTF or N-terminal (243-441) tau/CTF cDNA was used to produce recombinant tau protein in Escherichia coli BL21 (DE3) (Merck Millipore). Purification was performed as described previously (Hasegawa et al. ...
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bioRxiv - Microbiology 2022Quote: ... coli and inserted between the BamHI and XhoI sites in the multicloning site of the pET21a plasmid (Novagen, Madison, WI). The recombinant protein ...
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bioRxiv - Molecular Biology 2022Quote: ... coli cells diluted in M63 minimal medium as above were incubated with or without 1.5 mM hydrogen peroxide (Sigma-Aldrich) or 60 µg/mL phleomycin (InvivoGen ...
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bioRxiv - Microbiology 2022Quote: ... coli was grown overnight at 37°C and 200 rpm in LB broth (L7275-500TAB, Sigma-Aldrich, St. Louis, MO) from a colony scrape taken off an LB agar plate ...
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bioRxiv - Neuroscience 2022Quote: WT and TG-G2019S mice were exposed to intraperitoneal (ip) injections of a low dose of lipopolysaccharide LPS (0,1 mg kg−1 Escherichia coli serotype O111:B4) (Sigma-Aldrich), administrated twice a week for 12 weeks (Supplementary Figure 1) ...
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bioRxiv - Immunology 2022Quote: ... On day 7 differentiated macrophages were divided into six-well plates at a density of 0.8×106 cells/well in a total volume of 3 ml in MGM diluted to contain 10% L929 cell-conditioned medium (MGM 10/10) and primed for ∼18hrs with 100 ng/ml O26:B6 Escherichia coli LPS (Sigma).
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bioRxiv - Immunology 2024Quote: ... was diluted in pre-warmed complete RPMI to a final concentration of 0.5ug/ml and 1.0ug/mL and was applied for 4 hours prior to lipopolysaccharide (LPS, from Escherichia coli O111:B4, Sigma-Aldrich) treatment ...