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Citations for Millipore Sigma :
3001 - 3050 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 aa 1000 1200 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Bound proteins were detected by western blot using anti-His antibody and streptavidin-HRP conjugate (SIGMA, reference GERPN1231).
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bioRxiv - Zoology 2020Quote: ... recombinant RhATG5 and RhATG5191-199Δ were affinity-purified using Ni-NTA His•Bind Resin (Merck-Millipore, Darmstadt, Germany). Recombinant RhATG5 and RhATG5191-199Δ were then incubated with 10µg μ-calpain (Merck ...
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bioRxiv - Biochemistry 2020Quote: ... The proteins were cleaved by incubation with 1.5 % (w/w) His-tagged HRV-3C protease (Millipore Sigma SAE0045) or 15 U/mg His-tagged SUMO protease (Millipore Sigma SAE0067 ...
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bioRxiv - Biochemistry 2021Quote: A C-terminal 10x His-tagged Synechocystis PCC 6803 ocp gene (slr1963) was cloned in a pCDFDuet (Novagen), resulting in a plasmid named pEP4 ...
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bioRxiv - Cell Biology 2020Quote: Fixed cells were permeabilized using ice-cold Hi-C lysis buffer (10 mM TRIS-HCl pH 8 [Sigma] ...
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bioRxiv - Bioengineering 2021Quote: ... aliquots of each fraction were visualized via immunoblotting (anti-His antibody; Cat: SAB2702218, Sigma Aldrich Canada, Oakville, Canada) to identify fractions enriched for RBD ...
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bioRxiv - Cell Biology 2022Quote: ... HCP-1 was produced similarly but in Hi-5 cells maintained in EX-Cell 405 medium (Sigma-Aldrich) and collected after 66h infection ...
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bioRxiv - Molecular Biology 2024Quote: ... The peak corresponding to mono-SPY-His-tagged streptavidin tetramer was collected and concentrated using Amicon 10K (Millipore). The concentrated mono-SPY-His6-tagged streptavidin tetramer was further purified through Hiload superdex75 (Cytiva ...
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bioRxiv - Bioengineering 2023Quote: ... His-tagged GA-MatryoshCaMP6s were eluted in 1.5 mL 250 mM imidazole (Sigma-Aldrich; CAS 1467-16-9) 20 mM MOPS pH 7.0 ...
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bioRxiv - Microbiology 2023Quote: ... The fractions containing His-SSRP1 were concentrated with an Amicon centrifugal filter unit (50 kDa cut-off, Millipore) and buffer exchanged into storage buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Pathology 2023Quote: ... Then 2.4 µL of multiplexed PCR products were mixed with 9.35 µL of Hi-Di formamide (Sigma-Aldrich) and 0.15 µL of Genescan 500 Liz internal line size standard (Applied Biosystems ...
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bioRxiv - Microbiology 2023Quote: ... Fractions containing the His-tagged nucleotidases were pooled and concentrated using an Amicon 10 kDa MWCO filter (Millipore). The protein was buffer-exchanged into gel filtration buffer (100 mM HEPES pH 7.4 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Membranes were then incubated with anti-His primary antibody 1:5000 in 4% milk in PBS-T (Sigma) for 2 hours ...
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bioRxiv - Biochemistry 2024Quote: ... and lysates were clarified by ultracentrifugation (20,000 x g for 20 min) and incubated with 0.4 mL of His-Select nickel resin (Sigma) for 1 h at 4°C with gentle mixing ...
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bioRxiv - Biochemistry 2024Quote: ... and the soluble fraction was purified by affinity chromatography (either Ni2+ or Strep) (His-Select resin (Sigma-Aldrich); Strep-Tactin®XT 4Flow resin (IBA Lifesciences)) ...
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bioRxiv - Biochemistry 2024Quote: Cleaved MntR was loaded onto a 5 ml column of Ni-NTA His•Bind® Resin (Millipore Sigma) equilibrated with Buffer A and then eluted with 40 mM imidazole ...
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bioRxiv - Microbiology 2022Quote: ... EIF4E2 shRNA#2 (sh4EHP#2) (Sigma, TRCN0000280916) and EIF4E2 shRNA#3 (sh4EHP#3 ...
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bioRxiv - Microbiology 2022Quote: ... GIGYF2 shRNA#2 (shGIGYF2#2) (Sigma, TRCN0000138937) and GIGYF2 shRNA#3 (shGIGYF2#3 ...
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bioRxiv - Cell Biology 2021Quote: ... 2.5% 2-Mercaptoethanol (Sigma, 60-24-2), and sonicated ...
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bioRxiv - Neuroscience 2019Quote: ... 2-DG (2 g/kg; Sigma, Ireland), and insulin (11.5 IU/kg (400 μg/kg) ...
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bioRxiv - Cell Biology 2021Quote: ... 0.1 mM 2-mercaptoethanol (2-ME; Sigma), 100 U/ml penicillin and 100 µg/ml streptomycin sulfate (Nacalai Tesque ...
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bioRxiv - Cancer Biology 2020Quote: ... with 2% v/v 2-mercaptoethanol (Sigma). The samples are immediately frozen on dry ice and subsequently stored at −80° C until library preparation and sequencing ...
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bioRxiv - Neuroscience 2021Quote: ... 1% 2-methyl-2-propanethiol (Sigma 109207), 0.01% acetophenone (Sigma W200910) ...
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bioRxiv - Neuroscience 2021Quote: ... 1% 2- methyl-2-thiazoline (Sigma M83406), 1% citronellol (Sigma W230915) ...
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bioRxiv - Molecular Biology 2021Quote: 2-deoxyglucose (2-DG, Sigma, D6134-250MG) was dissolved in 1x DPBS ...
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bioRxiv - Cell Biology 2021Quote: ... 2-5mM of 2-deoxy-glucose (SIGMA) was added to the normal medium ...
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bioRxiv - Cancer Biology 2022Quote: MI-2-2 was purchased from Millipore; MI-503 ...
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bioRxiv - Plant Biology 2022Quote: 2-mercaptoethanol (2-ME, Sigma-Aldrich, M6250)
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bioRxiv - Neuroscience 2023Quote: ... 4uL 2-Mercaptoethanol (Sigma #60-24-2), supplemented with 100µg/mL Normocin (InvivoGen #ant-nr-2) ...
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bioRxiv - Neuroscience 2023Quote: ... 2-Aminoethyldiphenylborinate (2-APB, 100 μM; Sigma), Larixyl acetate (10 μM ...
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bioRxiv - Bioengineering 2024Quote: ... 2-hydroxy-2-methylpropiophenone (Sigma-Aldrich, Netherlands) was added to the hydrogel solution at a volume ratio of 1:100 to achieve efficient crosslinking ...
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bioRxiv - Plant Biology 2023Quote: ... 2% Phosphatase inhibitor cocktail 2 (Sigma-Aldrich) and 2% Phosphatase inhibitor cocktail 3 (Sigma-Aldrich)] ...
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bioRxiv - Neuroscience 2023Quote: ... and 2-Butanone (2-Bt; Sigma Aldrich).
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bioRxiv - Cell Biology 2023Quote: 2-deoxyglucose (2-DG, Sigma, D6134-250MG) was dissolved in 1x DPBS ...
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bioRxiv - Biophysics 2023Quote: ... 2-Aminoethoxydiphenyl borate (2-APB, Sigma-Aldrich) was dissolved in DMSO to produce a 1 M stock ...
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bioRxiv - Cancer Biology 2024Quote: ... 2-Deoxy-Glucose (2-DG, Sigma Aldrich).
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bioRxiv - Molecular Biology 2021Quote: ... rabbit anti-V5 (PRB-189P, Covance, 1/1000) or mouse anti-FLAG (M2, Sigma, 1/1000) antibodies ...
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bioRxiv - Neuroscience 2022Quote: ... We used two different antibodies for both PV (Sigma, P3088, 1:1000; Swant, PV27, 1:1000) and CB (Swant ...
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bioRxiv - Genetics 2021Quote: ... and 45 mg/kg body weight of Evans blue dye (Sigma-Aldrich, St. Louis, MO; E-2129) and saline was injected via the femoral vein ...
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bioRxiv - Developmental Biology 2021Quote: ... plates or coverslips were coated with 100 μg/ml poly-D-lysine (Merck Millipore, A-003-E) at 37°C overnight ...
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bioRxiv - Genomics 2020Quote: ... Cells were released from G1 by washing with YPD with 0.15 mg/mL pronase E (Sigma Aldrich) three times ...
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The tumour microenvironment shapes dendritic cell plasticity in a human organotypic melanoma culturebioRxiv - Immunology 2019Quote: ... bovine serum albumin lipid mix (palmitic acid 25 µmol/l; arachidonic acid 7 µmol/l; linoleic acid 15µmol/l; vitamin E 0.4µg/ml; all from Sigma), insulin (0.1 µmol/l ...
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bioRxiv - Microbiology 2021Quote: ... Lung paraffin sections were stained with Hematoxylin and Eosin (H&E) or Gomori’s Methenamine silver (GMS) (Sigma) for pathological examination ...
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bioRxiv - Immunology 2022Quote: ... and longitudinal sections were stained with H&E and Oil Red O (Millipore Sigma; Cat No O0625) to visualize BM adiposity ...
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bioRxiv - Neuroscience 2022Quote: ... Skin sections were processed for morphometric analysis using hematoxylin and eosin (H&E) (Sigma-Aldrich, #MHS32-1L) and immunofluorescence staining (e.g. ...
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bioRxiv - Immunology 2022Quote: ... paraffin embedded and sections (5 µm) prepared and stained with hematoxylin and eosin (H&E; Sigma-Aldrich). For clinical scoring 4-6 random field of views at 10x magnification from three sections 500 µm apart were assessed per colon ...
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bioRxiv - Cell Biology 2022Quote: ... 4 μm sections of paraffin-embedded samples were stained with hematoxylin and eosin (H&E, Sigma-Aldrich), periodic acid-Schiff (PAS ...
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bioRxiv - Biochemistry 2021Quote: ... Detection of HRP conjugates was carried out with 3,3′,5,5′-tetramethybenzidine (TMB/E, Millipore, Billerica, MA, USA). Detection of AP conjugated antibodies was carried out with SIGMAFAST p-nitrophenyl phosphate tablets (Sigma-N2770).
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bioRxiv - Cell Biology 2021Quote: ... The separated proteins were then transferred to the PVDF membranes (0.45 µm Immobilon®-E; Merck Millipore). Membranes were blocked either with 2.5% nonfat milk in TBST (50 mM Tris ...
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bioRxiv - Immunology 2020Quote: E-liquid mixture of 70% propylene glycol and 30% glycerol and 6mg/mL nicotine (purchased from Sigma) without flavors or additives were used ...