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Citations for Millipore Sigma :
2951 - 3000 of 10000+ citations for SARS CoV 2 Spike Glycoprotein S2 aa 1000 1200 His Tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Evolutionary Biology 2021Quote: Total RNA of the collected/dissected petals (see supplementary fig. S2) was extracted using Spectrum Plant Total RNA Kit (Sigma-Aldrich, St. Louis, MO, USA). cDNA was synthesized following the methods described in Supporting Information Methods S4 in Lin and Rausher (2021) ...
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bioRxiv - Microbiology 2020Quote: Standard and previously described protocols were used for PCR amplification (a full list of PCR primers is provided in Table S2; all of them were obtained from Eurofins Genomics, Ebersberg, Germany or Sigma-Aldrich, St. Louis, MO, USA), bacterial transformation (E ...
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bioRxiv - Microbiology 2020Quote: ... All transformants were screened by PCR using gene-specific primers (Table S2, Fig. S2c) and were verified by Southern hybridization analyses (DIG DNA Labeling and Detection Kit, Sigma-Aldrich, St. Louis, MO, USA) (Fig ...
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bioRxiv - Bioengineering 2020Quote: ... 2-hydroxy-4’-(2-hydroxyethoxy)-2-methylpropiophenone (Irgacure 2959, 410896, Sigma Aldrich), VEGF specific control aptamer (47-nt ...
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Hypothalamic melanocortin-4 receptors on astrocytes mediate inflammation and body weight homeostasisbioRxiv - Neuroscience 2022Quote: ... mice were deeply anesthetized with avertin (2, 2, 2-tribromoethanol; Sigma-Aldrich) prior to blood draw via cardiac puncture followed by transcardial perfusion with 10 mL of 10% sucrose in 1X Phosphate Buffered Saline (PBS) ...
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bioRxiv - Synthetic Biology 2021Quote: ... The membranes were blocked and incubated with specific antibodies: HA-tagged spike protein was detected with anti-HA antibody (H9658, Sigma-Aldrich, 1:10,000), hACE2 was detected with anti-ACE2 antibody (sc-390851 ...
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bioRxiv - Genomics 2023Quote: ... used for spike-in controls for ChIPs of TWIST1 depletions were cultured in Complete ES Cell Medium with 15% FBS (Millipore, ES-101-B), 25 ng/ml bFGF ...
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bioRxiv - Cell Biology 2020Quote: ... coli) and cloned into the HindIII and EcoRI sites of pTAC-MAT-Tag2 (Sigma-Aldrich) to yield the pTac-Cat plasmid ...
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bioRxiv - Cell Biology 2020Quote: ... LPS (lipopolysaccharides from Escherichia coli O55:B5) was from Sigma-Aldrich (St. Louis, MO, USA).
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bioRxiv - Molecular Biology 2020Quote: ... coli and the cDNAs were synthesized by IDT and were cloned into pCDF duet (Novagen) in frame with an N terminal 6 x HIS tag for bacterial expression ...
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bioRxiv - Immunology 2019Quote: ... containing LPS from Escherichia coli O55:B5 at 40 mg/ml (Sigma Chemical Co, St) (Louis ...
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bioRxiv - Bioengineering 2020Quote: ... coli cultures were grown at 37 °C in LB (both broth and solid) (Sigma, US). Appropriate antibiotics were supplemented with the following working concentrations ...
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bioRxiv - Neuroscience 2020Quote: ... coli 0111:B4 lipopolysaccharide (LPS) (Sigma-Aldrich, cat#L3012, Ec#297-473-0, lot#WI150506) was dissolved in saline and used at a dose of 0.1 mg/kg ...
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bioRxiv - Microbiology 2020Quote: ... Escherichia coli strains were grown in liquid or solid Luria Bertani (LB) medium (Sigma Aldrich) at 37°C (57) ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli in Lennox’s Lysogeny Broth (LB-Lennox) medium (10 g L−1 peptone (EMD Millipore), 5 g L−1 yeast extract (EMD Millipore) ...
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bioRxiv - Synthetic Biology 2021Quote: ... coli DH5α strains were routinely cultivated in lysogeny broth (LB) medium (LB Broth, Sigma Aldrich), 37°C ...
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bioRxiv - Genomics 2020Quote: ... anaesthetised snails were injected as above with 0.5 mg/mL lyophilised Escherichia coli (Sigma-Aldrich, Steinheim ...
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bioRxiv - Neuroscience 2020Quote: ... Antibodies against adenomatous polyposis coli (1:250; APC or CC1, Merck Millipore, Billerica, MA, USA) or GFP (1:8000 ...
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bioRxiv - Microbiology 2021Quote: ... coli O111:B4 (Catalog# L2630-10MG, lot 114M4009V) were purchased from Sigma (St. Louis, MO). Budesonide (Catalog# S1286) ...
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bioRxiv - Molecular Biology 2021Quote: ... coli wild type (wt) Hfq protein and its mutants were expressed from pET15b vector (Novagen) and purified as described [38] ...
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bioRxiv - Cell Biology 2021Quote: ... coli DH10B cells and cells harboring recombinant plasmids selected on Luria Bertani (LB; Sigma-Aldrich) agar plates with 50 μg/ml kanamycin ...
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bioRxiv - Molecular Biology 2022Quote: ... coli HMS174 (DE3) (F- recA1 hsdR(rK12- mK12+) Rif R (DE3)) was purchased from Novagen. E ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... as previously performed,24 γ-irradiated LPS from Escherichia coli O111:B4 (BioXtra; Sigma-Aldrich) dissolved in PBS was IV-injected at the dose of 2.4 ng ...
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bioRxiv - Molecular Biology 2020Quote: ... coli ProQ protein or its N-terminal domain (NTD) were cloned into pET15b vector (Novagen) using BamHI restriction site (Supplemental Table S2) ...
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bioRxiv - Microbiology 2021Quote: ... coli membrane fractions were determined using a bicinchoninic acid (65) protein assay kit (Sigma-Aldrich) with BSA (Interchim ...
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bioRxiv - Biochemistry 2020Quote: ... coli cells by the heat-shock approach as described in the supplier’s manual (Novagen/Agilent) and by antibiotic selection using Zeocin (Invitrogen/ThermoFisher) ...
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bioRxiv - Biochemistry 2021Quote: RIG-I was expressed using pET28 SUMO vector in Escherichia coli strain Rosetta (DE3) (Novagen). Soluble cell lysate was purified using a Ni2+-nitrilotriacetate (QIAGEN ...
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bioRxiv - Immunology 2020Quote: ... To stimulate cells 0.3 ng* μl−1 LPS (Lipopolysaccharide from Escherichia coli 0111:B4, Sigma) was added and cells were incubated for 4 hours at 37°C and 7 % CO2 ...
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bioRxiv - Microbiology 2021Quote: ... The BMDMs were primed with 100 ng/mL O26:B6 Escherichia coli LPS (Sigma®) and incubated overnight at 37°C with 5% CO2.
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bioRxiv - Biochemistry 2022Quote: ... The cloned vector was transformed into Escherichia coli BL21 HMS174(DE3) chemically competent cells (Novagen). A colony was picked and placed into an overnight outgrowth ...
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bioRxiv - Neuroscience 2022Quote: ... anti-adenomatous polyposis coli clone CC1 (APC/CC-1) (1:100, mouse, Millipore Cat# OP80) anti-platelet derived growth factor receptor alpha (PDGFRa ...
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bioRxiv - Evolutionary Biology 2024Quote: ... coli C wildtype and phage resistant strains were diluted 10 times in Ringer’s solution (Sigma). 50 µL of each 10 times diluted overnight culture (5 x 106 cells ...
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bioRxiv - Neuroscience 2024Quote: ... we used activated microglia induced by lipopolysaccharides (LPS, Cat# Escherichia coli O111:B4, Sigma-Aldrich) in this study ...
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Antimicrobial and antibiofilm activity of human recombinant H1 histones against bacterial infectionsbioRxiv - Microbiology 2024Quote: Histones H1.2 was cloned into pET21a and expressed in Escherichia coli BL21(DE3) (Merk Millipore) while H1.0 and its C-terminal domain (CTD ...
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bioRxiv - Cell Biology 2023Quote: Human αSN cDNA was transformed into Escherichia coli Strain BL21 (DE3) (Novagen, Madison, Wis., USA) using the pNIC28-Bsa4 plasmid ...
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bioRxiv - Biophysics 2023Quote: Chaperonin 60 lyophilized powder (GroEL, 1 mg) from Escherichia coli was purchased from Sigma-Aldrich (Cat # C7688 ...
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bioRxiv - Immunology 2023Quote: ... Lipopolysaccharides (LPS) Escherichia coli O111:B4 origin (L2630) and Evans blue (E2129) procured from Sigma, USA ...
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bioRxiv - Microbiology 2023Quote: ... Escherichia coli cells were counter-selected using Thiosulfate citrate bile salts sucrose (TCBS, Sigma-Aldrich) agar ...
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LRBA balances antigen presentation and T-cell responses via autophagy by binding to PIK3R4 and FYCO1bioRxiv - Immunology 2024Quote: ... followed by stimulation with LPS from Escherichia coli 055: B5 (20 µg/ml, Sigma-Aldrich) for 3 days in complete RPMI medium (L-glutamine ...
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bioRxiv - Microbiology 2024Quote: ... The BMDMs were primed with 100 ng/mL O26:B6 Escherichia coli LPS (Sigma®) and incubated overnight at 37°C with 5% CO2.
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bioRxiv - Immunology 2024Quote: LPS from Escherichia coli O111:B4 was obtained from Sigma-Aldrich (St. Louis, MO, USA). Dexamethasone (Dx ...
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bioRxiv - Immunology 2024Quote: ... the cells were treated with 10ng/ml LPS (Lipopolysaccharides from Escherichia coli O55:B5; Sigma). After 3 hr ...
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bioRxiv - Molecular Biology 2024Quote: ... were expressed in Escherichia coli BL21 (DE3) slyD- cells [56] carrying the plasmid pRARE2 (Novagen). Recombinant GST (vector pGEX-4T2 (Amersham) ...
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bioRxiv - Neuroscience 2021Quote: ... and then supplemented with 2% w/v of 2-Hydroxy-4’-(2-hydroxyethoxy)-2-methylpropiophenone (Sigma Aldrich 410896) as the UV initiator ...
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bioRxiv - Developmental Biology 2021Quote: ... The obtained antisera were affinity-purified with recombinant Emi2-His protein electroblotted onto a membrane (Immobilon; EMD Millipore).
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bioRxiv - Molecular Biology 2020Quote: ... Sph2-YM4271 cells were grown on synthetic dropout (SD)-His media supplemented with the inhibitor 3 mM 3-amino-1,2,4-triazole (3-AT; Sigma). All Y1H effector plasmids were separately transformed into the Sph2-YM4271 reporter strain and plated on SD/-Leu plates ...
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bioRxiv - Plant Biology 2019Quote: ... The transformants were then spotted on SD (-Trp -Leu -His) selection media containing 0.5/1mM 3-Amino-1,2,4-triazole (3-AT; Sigma, A8056). The positive interactors were then scored based on the stringency of the selection.
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bioRxiv - Plant Biology 2019Quote: ... at least four independent colonies were used for yeast two-hybrid assay on SD-Leu-Trp-His plates with and without 3-amino-1,2,4-triazole (Sigma). Colonies were imaged every 24 h post plating for 4-5 days.
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bioRxiv - Molecular Biology 2020Quote: ... Membranes were then incubated for 4 hours with anti-His hexahistidine antisera conjugated to horseradish peroxidase (HRP) (Sigma) diluted in TBST at a ratio of 1:5000 ...
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bioRxiv - Molecular Biology 2022Quote: CobB driven deacetylation of His-PrsAc was analyzed by Western blot with anti-acetyl lysine antibodies (Sigma/Merck) and mass spectrometry ...