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Citations for Millipore Sigma :
2751 - 2800 of 10000+ citations for Rabbit Anti Borrelia burgdorferi sensu stricto B31 CRASP 1 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... Slides were then washed five times with PBS-BSA at 25°C followed by incubation in a 1:200 dilution of the tetramethylrhodamine-isothiocyanate-labelled goat anti-rabbit antibody (TRITC, Sigma-Aldrich) and fluorescein isothiocyanate-labelled goat anti-mouse antibody (FITC ...
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bioRxiv - Neuroscience 2022Quote: ... the tissue was permeabilized/blocked in a solution containing 1% Triton-X and 10% NGS in PBS for 30 min and then incubated with an anti-HNE-Michael adducts rabbit polyclonal antibody (1:150 dilution, EMD Millipore) in a 10% NGS ...
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bioRxiv - Cell Biology 2023Quote: ... the cells were washed with PBS and incubated with secondary antibodies at 1:500 dilution (goat anti-rabbit Alexa 594, Sigma Aldrich,) for 1h at room temperature ...
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bioRxiv - Plant Biology 2024Quote: ... 2003) and a goat anti-rabbit IgG secondary antibody conjugated with alkaline phosphatase (1:4000, Chemicon®, Sigma-Aldrich, www.sigmaaldrich.com, #AP307P). Detection by chemiluminescence with Immun-Star AP Substrate (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... The blots were washed thrice with TBST buffer and incubated with HRP-conjugated anti-rabbit antibodies (dilution 1:20000; Sigma-Aldrich) at 25°C for 1 h ...
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bioRxiv - Neuroscience 2023Quote: ... Poly-ubiquitinated CHTs were detected by stripping the membranes with Restore Plus buffer (Pierce) and incubating them with 1:2000 diluted rabbit anti-CHT polyclonal antibody (ABN458; EMD Millipore). The resulting chemiluminescent signal was acquired with a Chemidoc Touch Imaging System (Bio-Rad) ...
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bioRxiv - Physiology 2023Quote: ... washed three times with PBS-0.05% tween and followed by incubation with secondary antibodies directed against goat anti-mouse or rabbit HRP conjugated (1:5000, Sigma-Aldrich). The visualization of immunoreactive bands was performed using the enhanced chemiluminescence plus western blotting detection system (GE Healthcare) ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were then incubated in anti-TH monoclonal rabbit IgG primary antibody (1:2000 overnight at 4°C; Sigma-Aldrich) in solution with PBS and normal goat serum (NGS) ...
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bioRxiv - Synthetic Biology 2023Quote: ... in TBS-T for 1 h at room temperature and stained overnight at 4 °C with a rabbit anti-FLAG antibody (1:1000 dilution, Millipore F7425). After removing unbound antibodies by three 5-minute washes with TBS-T ...
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bioRxiv - Neuroscience 2023Quote: ... then washed with PBS again and incubated for 48h with a rabbit anti-TH antibody (1:1000, Millipore Cat# AB152, RRID:AB_390204) at 4°C ...
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bioRxiv - Genetics 2023Quote: ... as previously described.17 The following primary antibodies were used for immunohistochemistry: rabbit anti 2A peptide (1:7500, ABS31, Sigma-Aldrich), rabbit anti Ki67 (1:60 ...
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bioRxiv - Genomics 2023Quote: ... and cells were resuspended in 100 μL dig-wash buffer containing 2 mM EDTA and a 1:50 dilution of rabbit anti-mouse antibody (Sigma, M7023) and incubated at RT for 1 hour with slow rotation ...
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bioRxiv - Plant Biology 2023Quote: ... Anti-rabbit secondary antibody conjugated with horse radish peroxidase enzyme was used in the ratio of 1:10000 (Sigma Aldrich, USA) and blots were visualized using chemiluminescent substrate as described above.
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bioRxiv - Physiology 2023Quote: ... in PBS (BSA/PBS-T) for 1 hour prior to washing with PBS and incubation with primary anti-TH rabbit polyclonal antibody (EMD Millipore) at 1:300 BSA/PBS-T overnight at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... Processing of brain and brain sections for TH immunohistochemistry was similar to the protocol described above except for primary (rabbit anti-TH antibody 1/200, Millipore ab152) and secondary (Alexa Fluor 555-conjugated donkey anti-rabbit ...
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bioRxiv - Cancer Biology 2023Quote: ... This was followed by incubation with an anti-EMILIN-1 rabbit polyclonal antibody (x400, HPA002822; Sigma Aldrich, Saint Louis, MO, USA) in Dako REAL Antibody Diluent at 4°C for 24 h ...
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bioRxiv - Developmental Biology 2023Quote: ... the sections were incubated overnight at RT with a rabbit polyclonal anti-pH3 antibody (1:500; Sigma; Cat#: H0412; RRID: AB_477043). Sections were then rinsed 3 times in TBS for 15 min each and incubated for 1 hour at room temperature with a Cy3-conjugated goat anti-rabbit IgG antibody (Jackson ImmunoResearch ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... then incubated for 30 min at RT with blocking buffer (1% Bovine Serum Albumin in 1X PBS) and further incubated for at least 1 hour at RT with rabbit anti-hACE2 antibody (SAB 3500978, Sigma-Aldrich) or rabbit anti-TMPRSS2 antibody (BS-6285R ...
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bioRxiv - Immunology 2024Quote: ... wells were incubated with 10 µg/mL of sera for approximately 1 hr at 20 °C then washed 4 times with PBS/T before the addition of 50 µL of 1:1000 dilution of goat anti-rabbit gamma-chain alkaline phosphatase-conjugated secondary antibody (Sigma-Aldrich) for 1 hr at 20 °C ...
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bioRxiv - Neuroscience 2024Quote: ... dorsal and ventral transverse slices were incubated with the following primary and secondary antibody combinations using the protocol described: PCP4 (1:300, rabbit anti-PCP4 #HPA005792, Sigma-Aldrich)/goat anti-rabbit IgG (Life Technologies ...
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bioRxiv - Cell Biology 2019Quote: Antibodies used in immunofluorescence and Western blot include: anti-MMP9 rabbit polyclonal antibody (# AB13458) and anti-GAPDH mouse monoclonal antibody (# CB1001) purchased from EMD Millipore (Billerica, MA); anti-Src rabbit antibody (# 2108) ...
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bioRxiv - Genetics 2020Quote: ... Protein– antibody interaction was detected with either anti-mouse or anti-rabbit peroxidase-conjugated mouse IgG antibody (Sigma-Aldrich, St Louis, MO, USA), using Amersham™ ECL Plus western blotting detection system (GE Healthcare Life Sciences ...
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bioRxiv - Molecular Biology 2021Quote: ... Chromosome were blocked in PBT containing 0.2% BSA and 5% goat serum and sequentially incubated with primary antibodies (mouse anti-PolII H5 IgM, 1:1000, Abcam, and rat anti-HA MAb 3F10, 1:50, Roche, or rabbit anti-FLAG, 1:1000, SIGMA) followed by incubation with Alexa488- and/or Alexa647-coupled secondary antibodies (Molecular Probes ...
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bioRxiv - Cell Biology 2020Quote: ... 1999), rabbit anti-HTP-1/2 (1:500) (Martinez-Perez et al., 2008) and mouse anti-FLAG (1:200) (Sigma).
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bioRxiv - Neuroscience 2023Quote: ... Samples were blocked in SureBloc (Lubio biosciences) for 1 hour followed by incubation with primary antibodies (anti Gpr133 antibody -Aviva Biosystems; anti Flag antibody: Sigma). HRP tagged Secondary antibodies ...
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bioRxiv - Developmental Biology 2020Quote: ... were washed in IP buffer and incubated with primary antibody (rabbit anti-HA, anti-myc, CST or mouse anti-flag, Sigma) or normal IgG as control (mouse IgG ...
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bioRxiv - Zoology 2019Quote: ... and then incubated overnight at 4°C in primary antibodies (mouse anti acetylated tubulin, Sigma T6793 or mouse anti tyrosinated tubulin, Sigma T9028 and rabbit anti serotonin, Sigma S5545 or rabbit anti FMRF-amide ...
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bioRxiv - Neuroscience 2019Quote: ... following by primary antibody incubation overnight at 4°C (rabbit anti-pRIPK3, Abcam; mouse anti-TH, Millipore; mouse anti-pMLKL: Millipore rabbit anti-TH ...
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Activation of XBP1s attenuates disease severity in models of proteotoxic Charcot-Marie-Tooth type 1BbioRxiv - Neuroscience 2024Quote: ... Peroxidase-conjugated secondary antibodies (anti-rabbit HRP, DAKO, P0448; anti-chicken IgG-peroxidase, Sigma; anti-mouse IgG peroxidase, A3682, Sigma) were visualized using Amersham ECL or ECL Prime 225 reagent (GE Healthcare ...
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bioRxiv - Molecular Biology 2024Quote: ... Secondary antibodies coupled to horse radish peroxidase (goat anti-rabbit, goat anti-rat, and goat anti-mouse) were purchased from Sigma and used at the recommended dilution.
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bioRxiv - Biophysics 2024Quote: ... the membrane was blocked with 1% BSA with 0.05% Tween-20 and reacted with an anti-tau antibody (RTM38, 1:2500) and anti-β-actin antibody (1:10,000; Sigma-Aldrich) at RT for 1 h (or 4°C O/N) ...
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bioRxiv - Microbiology 2023Quote: ... burgdorferi at an MOI of 50 for 18h alone or in the presence of 50mM N-acetyl cysteine (NAC; Sigma, A7250). Cells were collected in RIPA buffer (for immunoblotting ...
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bioRxiv - Cell Biology 2019Quote: ... Primary antibody was diluted 1:100 in blocking buffer (rabbit-α-LAMP1: 1:100; rabbit-α-mTOR (HPA071227, Sigma-Aldrich Co. LLC): 1:12.5 ...
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bioRxiv - Neuroscience 2020Quote: ... The primary antibodies anti-PECAM-1 (1:1000; MAB1398Z, EMD Millipore) and anti-GFAP (1:1000 ...
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bioRxiv - Cancer Biology 2024Quote: ... Mouse anti-FLAG (Anti-FLAG M2 mouse monoclonal antibody, F1804-1 Sigma-Aldrich, 1:1000), mouse anti-HA (Monoclonal Anti-HA antibody produced in mouse ...
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bioRxiv - Cell Biology 2020Quote: ... for 30 min at RT followed by 1 h incubation at RT with rabbit anti-FLAG antibody (1:500, Sigma-Aldrich, St. Louis, MO, USA) and mouse anti-SEC31A antibody (1:500 ...
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bioRxiv - Pathology 2024Quote: ... buffer the membranes were incubated for 1 hour at room temperature with anti-rabbit/mouse/goat IgG secondary antibody conjugated with horseradish peroxidase (1:5000; Sigma–Aldrich, St Louis, MO, USA). Signals was detected with Clarity enhanced chemiluminescence (ECL ...
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bioRxiv - Bioengineering 2023Quote: ... pH 7.4) for 1 h at room temperature and were subsequently incubated with primary antibodies rabbit anti-claudin-5 (Sigma-Aldrich, cat# SAB4502981, AB_10753223, 1:300), mouse anti-claudin-5 (Invitrogen ...
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bioRxiv - Molecular Biology 2023Quote: ... or anti-AGO1-N-coil (1:1000 in 5% milk in PBS-T) followed by anti-Rabbit antibody (1:10,000 [Sigma] in 5% milk in PBS-T). The anti-AGO1-N-coil antibody was detected with anti-Rabbit antibody conjugated to HRP (1:2,000 [Sigma] in 5% milk in PBS-T) ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated in primary antibody (Anti S100A9, 1:200, CST; Anti Iba-1, 1:200, Wako; Anti NeuN, 1:200, Millipore; Anti NF-κB p65, 1:200, CST) at 4 °C overnight and in secondary antibody (Goat Anti-rabbit 1:200 ...
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bioRxiv - Immunology 2024Quote: ... RRID:AB_10718401), rabbit polyclonal anti-GM130 (2ug/ml, Novus NBP2-53420G, RRID:AB_2890912) and rabbit anti-LAMP1 (1:100, Sigma-Aldrich L1418, RRID:AB_477157). Images were acquired using a Keyence BZ-X710 at 40X and 60X magnification ...
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bioRxiv - Cell Biology 2022Quote: ... rabbit anti-vinculin (1:1000;ThermoFisher Sientific mouse anti-α-tubulin (clone B-512; 1:5000; Sigma-Aldrich) were used as primary antibodies ...
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bioRxiv - Neuroscience 2019Quote: ... Tissue was then incubated in secondary biotinylated rabbit antibody to goat antibody (1:250, Sigma, B-7024) for 2 h ...
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bioRxiv - Neuroscience 2020Quote: ... Rabbit antibody against Parvalbumin (1:500, PA1-933, Thermo Fischer Scientific) and Mouse antibody against GAD67 (1:500, MAB5406, Sigma-Aldrich) in staining buffer (0.2% BSA ...
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bioRxiv - Neuroscience 2022Quote: ... the sections were incubated with a mixture of rabbit anti-P2X7R (1:500) and goat anti-IBA-I (1:1000)/mouse anti-GFAP (1:2000, #G6171, Sigma-Aldrich, St. Louis, MO)/rabbit anti-NeuN (1:2000 ...
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bioRxiv - Cell Biology 2023Quote: ... rabbit β-gal antibody (MP 559761, dilution 1/500), rabbit anti DsRed (clontech 632496, dilution1:200) for tdTomato detection and rabbit polyclonal anti-Laminin (Sigma-Aldrich, Cat# L9393, dilution 1:1000). Following washes in PBS-T (0.05% Tween20 in PBS) ...
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bioRxiv - Biochemistry 2023Quote: ... The blots were incubated with HRP-conjugated secondary antibodies (Pierce 31450 for rabbit anti-mouse IgG-HRP; Sigma A6154 for goat anti- rabbit IgG-HRP) before being visualization of protein signals using the ECL max kit (Bio-rad).
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bioRxiv - Molecular Biology 2023Quote: ... using anti-FLAG antibody for CD44 (1:1000 dilution of anti-Flag M2-HRP antibody; Sigma, A8592) and anti-GST antibody for GST-MSN (1:1000 dilution of anti-GST (2.6H1 ...
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bioRxiv - Biochemistry 2020Quote: ... rabbit SYM11 antibody (Sigma Aldrich) (1:1K) ...
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bioRxiv - Cell Biology 2021Quote: ... Rabbit IgG antibody (CS200581, Millipore) was used as a negative control and anti-Histone H3 (tri methyl K9 ...