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Citations for Millipore Sigma :
151 - 200 of 10000+ citations for Recombinant Mouse Melanoma Cell Adhesion Molecule His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2024Quote: ... of GST and His tagged proteins were subjected to buffer exchange using Amicon Ultra 0.5 ml 10 kDa cutoff columns (Millipore Sigma, UFC501024) with five sequential rounds of concentration performed by centrifugation at 14000 g and 4 °C for approximately 10 minutes and dilution with “EB base” (5x ...
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bioRxiv - Molecular Biology 2021Quote: Melanoma cells treated with ARN22089 for 6 or 24 h and lysed in RIPA buffer (EMD Millipore or an optimized cocktail (250 mM NaCL ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 ng/ml of mouse recombinant IL-4 (Sigma-Aldrich), and 0.5 μg/ml anti-CD180 (RP/14 ...
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bioRxiv - Developmental Biology 2023Quote: ... 1000 U/ml ESGRO recombinant mouse LIF protein (Sigma ESG1106), 2 μM PD0325901 (Selleckchem S1036 ...
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bioRxiv - Synthetic Biology 2023Quote: ... 103 units/mL ESGRO Recombinant Mouse LIF Protein (Millipore #ESG1107), 3.0 µM CHIR99021 (GSK-3 inhibitor ...
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bioRxiv - Molecular Biology 2023Quote: ... and 5 ng/ml mouse recombinant IL-4 (Sigma-Aldrich) to allow B cell activation and class switch recombination to IgG1 ...
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Deep Plasma Proteome Profiling by Modulating Single Nanoparticle Protein Corona with Small MoleculesbioRxiv - Bioengineering 2024Quote: ... Small molecules were ordered from Sigma, Abcam ...
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bioRxiv - Cell Biology 2023Quote: The small molecule MDL-800 (Sigma) was used at a concentration of 20 μM to activate SIRT6 ...
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bioRxiv - Cell Biology 2024Quote: ... All small molecules were from Sigma Chemical Co. ...
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bioRxiv - Developmental Biology 2024Quote: ... Large dextran molecules (2 MDa, Sigma) were dissolved in growth medium in varying concentrations to generate varying osmotic pressures82 ...
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bioRxiv - Systems Biology 2020Quote: ... cells were treated with small molecules or vehicle (DMSO, SIGMA life science #D265O) for 1h ...
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bioRxiv - Microbiology 2021Quote: ... and extensively dialysed against HEPES-buffered saline and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag mAb, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (Bethyl).
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bioRxiv - Cell Biology 2022Quote: ... and extensively dialysed against HBS and their level of expression determined by ELISA using a mouse monoclonal anti-His antibody (His-Tag monoclonal antibody, 70796, EMD Millipore) as primary antibody and a goat anti-mouse alkaline phosphatase-conjugated secondary (A3562 ...
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bioRxiv - Molecular Biology 2021Quote: ... Epitope tagged PARP1 was detected by mouse anti-FLAG antibody (# F3165, Millipore-Sigma) and visualized with an anti-mouse IgG coupled with Alexa Fluor 594 dye (Thermo Fisher Scientific # A-11005) ...
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bioRxiv - Physiology 2020Quote: ... or mouse anti-S-tag (for S-tagged NTD, 1:1500, Sigma, SAB2702204) and rabbit anti-Nav1.5 (1:150 ...
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bioRxiv - Molecular Biology 2021Quote: ... Epitope tagged PARP1 was detected by mouse anti-FLAG antibody (# F3165, Millipore-Sigma) and visualized with an anti-mouse IgG coupled with Alexa Fluor 594 dye (Thermo Fisher Scientific # A-11005) ...
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bioRxiv - Cell Biology 2022Quote: ... cell de-adhesion dynamics was quantified by performing live cell time lapse imaging following treatment with 0.5mM EDTA (Sigma-Aldrich). For the experiment ...
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bioRxiv - Biophysics 2023Quote: ... then incubated with mouse anti-His tag primary antibody (Millipore Sigma SAB1305538) at 1:1,000 dilution ...
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bioRxiv - Cell Biology 2021Quote: For conjugation of SNAP-tagged proteins (paxillin, kindlin) recombinant proteins to be labeled with fluorophores were concentrated using Amicon Ultra Centrifugal Filter units (Millipore) to ∼50 μM ...
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bioRxiv - Cell Biology 2023Quote: ... GST-tagged recombinant protein was eluted by three sequential incubations of GSH-beads with 50mM reduced L-GSH (SIGMA, G4251) in 100mM Tris-HCl [pH8.0],150mM NaCl ...
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bioRxiv - Developmental Biology 2019Quote: ... Cadherin-dependence of cell adhesion was tested after either depleting calcium by adding EGTA (5 mM, Sigma) to the medium ...
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bioRxiv - Plant Biology 2019Quote: ... The recombinant proteins with either FLAG-tag or His-tag were purified by corresponding immune affinity agarose beads (Sigma).
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bioRxiv - Cancer Biology 2024Quote: Cell lines used in this study including 293T cell line (CRL-3216), B16-OVA cell line (murine melanoma, SCC-420) from Millipore Sigma, MC38 cell line (CVCL-B288) ...
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bioRxiv - Microbiology 2022Quote: ... with the following modifications: Mouse anti-GST tag or mouse anti-His tag 1:200× (Sigma-Aldrich) and HRP-conjugated goat anti-mouse IgG 1:2,000× (Seracare Life Sciences ...
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bioRxiv - Cancer Biology 2023Quote: ... 500,000 freshly dissociated melanoma cells were infected with viral supernatant and supplemented with 10 µg/mL polybrene (Sigma). The following day ...
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Transcriptional Isoforms of NAD+ Kinase regulate oxidative stress resistance and melanoma metastasisbioRxiv - Cancer Biology 2023Quote: ... 500,000 freshly dissociated melanoma cells were infected with viral supernatant and supplemented with 10 μg/mL polybrene (Sigma). The following day ...
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bioRxiv - Neuroscience 2024Quote: ... Nuclei from tagged cells were separated from non-tagged nuclei by incubation with anti-GFP (Sigma; cat#SAB4301138-100UL) and anti-myc (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... Melanoma Organoid Complete Media formula [18]: 250 mL DMEM (Sigma D6429), 125 mL Nutrient Mixture Ham’s F12 (Sigma N4888) ...
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bioRxiv - Cell Biology 2024Quote: ... respectively (Millipore Cat# HI-14K, HI-13K). Samples treated with 3 mM glucose KRBH were measured undiluted ...
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bioRxiv - Microbiology 2021Quote: ... The plates were incubated with anti-mouse IgG conjugated with alkaline phosphatase (whole molecule) (Sigma-Aldrich A-3688) diluted 1:2000 ...
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bioRxiv - Bioengineering 2022Quote: ... a 96-well plate was seeded with 1×104 L929 murine fibroblast cells per well and incubated for 24 h to allow for cell adhesion and spreading (L929 cell line was purchased from Sigma-Aldrich, Poznań ...
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bioRxiv - Cell Biology 2019Quote: ... 1000 U/mL mouse recombinant Leukemia inhibitory factor (LIF, ESG1107, Millipore) and Penicillin-Streptomycin-Neomycin Antibiotic Mixture (15640055 ...
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bioRxiv - Immunology 2020Quote: ... and 5 ng/ml of mouse recombinant IL-4 (Sigma-Aldrich) for CSR to IgG1 ...
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bioRxiv - Immunology 2024Quote: ... and 5 ng/ml of mouse recombinant IL-4 (Sigma-Aldrich) (L-I) ...
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bioRxiv - Developmental Biology 2023Quote: ... 103 units/mL ESGRO recombinant mouse LIF protein (Millipore Sigma, ESG1107), 2 μM PD0325901 (Tocris ...
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bioRxiv - Immunology 2020Quote: ... cells were stained for surface molecules prior to fixation in 4% paraformadehyde (Sigma Aldrich) for 20 minutes ...
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bioRxiv - Immunology 2023Quote: ... The pull-downed proteins were separated by SDS-PAGE on 10-to-20% gradient gel (ATTO) and the recombinant FLAG- tagged WT sCTLA-4 was detected by anti-FLAG M2 antibody (Sigma-Aldrich).
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bioRxiv - Microbiology 2020Quote: ... FLAG-tagged ANP32 proteins were detected using mouse anti-FLAG M2 primary antibody (Sigma), followed by goat anti-mouse Alexa Fluor 568 (Invitrogen) ...
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Transcription factor LSF facilitiates lysine methylation of α-tubulin by microtubule-associated SET8bioRxiv - Biochemistry 2019Quote: ... the epitope tagged LSF was detected by mouse anti-FLAG antibody (F3165, Sigma-Aldrich) and visualized with an anti-mouse IgG coupled with Alexa Fluor 488 (Molecular Probes) ...
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bioRxiv - Systems Biology 2024Quote: ... Tagged proteins were detected using mouse α-Flag M2 (Sigma Aldrich F3165, 1:10,000), mouse α-V5-tag (Thermo Fisher R960-25 ...
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bioRxiv - Molecular Biology 2023Quote: ... and then with a secondary mouse anti-rabbit IgG tagged with FITC (Millipore, MA). Positively stained areas were quantified and expressed as positive area (μm2)/total area (mm2).
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bioRxiv - Plant Biology 2020Quote: Three molecules of chitosan (Sigma - Aldrich®) of different molecular weight (Supplementary Table 1 ...
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bioRxiv - Immunology 2019Quote: ... or a specific target molecule (Sigma-Aldrich) was transfected into HEK293T cells together with psPAX2 and pMD2.G with Lipofectamine 2000 ...
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bioRxiv - Immunology 2020Quote: ... or a specific target molecule (Sigma-Aldrich) was transfected into HEK293T cells together with psPAX2 and pMD2.G with Lipofectamine 2000 ...
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bioRxiv - Microbiology 2022Quote: ... small molecule SOX18 inhibitor SM4 (Sigma-Aldrich / or a kind gift from Gertrude Biomedical Pty Ltd. ...
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bioRxiv - Biophysics 2023Quote: ... Small molecules (L-DOPA (Sigma-Aldrich, D9628), L-Tyr (Sigma-Aldrich ...
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bioRxiv - Biophysics 2022Quote: ... Small molecule: Serotonin (H9523, Sigma-Aldrich, UK). Ampure XP magnetic beads (for purification of DNA barcoded probes ...
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bioRxiv - Cell Biology 2021Quote: A549 cells expressing GFP-tagged EGFR were obtained from Sigma. Cells were plated on Edge plates (Thermo Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... focal adhesion kinase-1 (FAK) inhibitor (PF 573228) from Sigma-Aldrich, ROCK Inhibitor (Y-27632 ...
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bioRxiv - Biochemistry 2021Quote: Metastatic Melanoma and lung cancer cell lines were cultured in RPMI 1640 (Sigma-Aldrich Co., St Louis, MO, USA) with 10% fetal bovine serum (FBS) ...