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Citations for Millipore Sigma :
1201 - 1250 of 8516 citations for V Bottom Microplate 96 well since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2024Quote: ... the lysates were separated on 8% v/v or 12% v/v polyacrylamide gels and transferred to a Polyvinylidene difluoride (PVDF) membrane (Millipore ISEQ00010). Membranes were incubated in 5% w/v milk buffer for 1 h at room temperature and incubated in primary antibody overnight at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... then diluted (5% volume per volume (v/v)) to a final concentration of 10 mg/mL in a suspension of 10% (v/v) Cremophor EL (Millipore 238470) and 85% (v/v ...
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bioRxiv - Biochemistry 2023Quote: ... Our DMEM formulation for storing cells in liquid nitrogen contained 20 % (v/v) FBS and 10 % (v/v) DMSO (cat# D8418, Sigma Aldrich). All reagents were warmed up to 37°C prior to usage.
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bioRxiv - Bioengineering 2022Quote: ... consisting of tris-buffered saline containing 0.03% v/v Triton X-100 and 10% v/v donkey serum (Millipore, s30-100ml). After blocking for 1 hr at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... for 5 minutes and suspended in medium (DMEM 85% v/v, Corning; FBS 15% v/v, VWR; 1x Penicillin-Streptomycin, VWR; 50 µM beta-mercaptoethanol, Sigma-Aldrich). 10,000 viable cells were plated per well in white polystyrene 384-well microplates (Greiner Bio-One ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... the formed cell colonies were fixed in PBS for 30 min using 3.4% v/v formaldehyde (4980.1, Carl Roth, Germany) and stained with 10% v/v of Giemsa solution (GS500, Sigma-Aldrich, USA) in Milli-Q water for 60 min ...
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bioRxiv - Plant Biology 2022Quote: ... 75 µm thick sections were stained for 60 minutes with 0.1 % w/v Direct Yellow 96 (Sigma Aldrich, St. Louis, US, S472409-1G) diluted in ClearSee [46] (10 % w/v xylitol ...
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bioRxiv - Genetics 2021Quote: ... 0.2% (w/v) glucose (Wako) and 0.2%(v/v) MEM amino acid (50x) solution (Sigma). When necessary ...
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bioRxiv - Microbiology 2020Quote: ... Membranes were blocked in 1% w/v skim milk 0.05% v/v Tween20 (Sigma-Aldrich) in PBS (hereby referred to as 1% milk PBS-T ...
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bioRxiv - Immunology 2020Quote: ... 20% (v/v) glycerol and 0.2% (w/v) bromophenol blue (all reagents from Sigma Aldrich). Samples were boiled for 3 min and sonicated for 30 s prior to loading on 4-20% Mini-PROTEAN TGX precast protein gels (Bio-Rad ...
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bioRxiv - Cell Biology 2021Quote: ... 10% (w/v) brain homogenate was incubated in 2% (v/v) sarkosyl (Sigma, 61747-100ML) and 0.5% (v/v ...
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bioRxiv - Physiology 2023Quote: ... Metabolites were extracted from 10 μl plasma by adding 90 μl of Acetonitrile:Methanol:Formic acid (74.9:24.9:0.2,v/v/v) solution spiked with valine-d8 (Sigma) and Phenylalanine-d8 (Cambridge Isotope Laboratories) ...
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bioRxiv - Developmental Biology 2023Quote: Zebrafish larvae were anesthetized with 1 mg/ml Tricaine and transferred to a 6-well glass-bottom plate containing 3% methylcellulose (Sigma-Aldrich, MO, USA) in E3 medium ...
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bioRxiv - Evolutionary Biology 2020Quote: ... cells lysis was done in a 96 deep-well plate by adding 450µl of lysis buffer containing 1M sorbitol (SIGMA-ALDRICH), 100mM EDTA and 0.45µl lyticase (10IU/µl) ...
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bioRxiv - Cancer Biology 2021Quote: Tail tip fibroblasts were seeded in 96-well plates and the mitochondrial membrane potential (MMP) was determined using the mitochondrial membrane potential kit (Sigma) following the recommendations made by the manufacturer ...
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bioRxiv - Microbiology 2020Quote: ... Stationary-phase cultures were diluted 1:10 000 into fresh 7H9 before spotting onto prepared 7H10 plates using a 96-well replicator pin (Sigma). All spotting was performed in triplicate ...
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bioRxiv - Immunology 2019Quote: ... by spinoculation in 96-wells plate (1,200 x g, 90 min at 16°C) with polybrene (4 μg/ml, EMD Millipore). 6h after infection / transduction ...
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bioRxiv - Immunology 2019Quote: ... Nunc Maxisorb 96-well plates were coated with 100µl poly-l-lysine H20 at 20 µg/ml (Sigma, cat # P4832) overnight at 4°C ...
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bioRxiv - Developmental Biology 2019Quote: ... and The Spectrum Collection (Microsource Discovery Systems, 2000 compounds) were arrayed in MultiScreen-Mesh 96-well culture receiver trays (Millipore) in columns 2-11 and diluted to 25 μM in E3 medium for drug screening ...
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bioRxiv - Evolutionary Biology 2021Quote: ... the PCR fragment was amplified with the primers detailed in Supplementary Table 1 and purified in MultiScreen TM Vacuum Manifold 96-well plates (Merck Millipore). The purified PCR fragment was incubated with either the enzyme NlaIII or water (negative control ...
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bioRxiv - Biochemistry 2020Quote: The S-variant and RBD coupled bead sets were incubated with SARS-CoV-2 patient sera at 3 dilutions (1/100; 1/1000 and 1/10000) for 1 h in 96-well filter plates (MultiScreenHTS; Millipore) at room temperature in the dark on a horizontal shaker ...
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bioRxiv - Biochemistry 2020Quote: ... progress was tracked by the change in absorbance at 340 nm by the formation or consumption of NADPH (ε340= 6,220 M-1 cm-1) in UV-transparent 96 well plates (Greiner, Sigma-Aldrich) measured using a SpectraMax Plus microplate reader (Molecular Devices).
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bioRxiv - Neuroscience 2020Quote: ... Sandwich ELISA kits for quantitative detection of human Aβ 1-40 peptides in a flexible 96 well format were purchased from Millipore (cat ...
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bioRxiv - Immunology 2021Quote: ... individual positive cells were sorted into the inner wells of a 96-well plate with 20 μl of pre-reverse transcription (RT) lysis mix containing 0.1 μl of NP40 (Sigma-Aldrich), 0.5 μl of RNAse Inhibitor (Thermo Fisher) ...
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bioRxiv - Microbiology 2020Quote: ... for ELISpot detection in combination with multiscreen immunoprecipitation (IP) ELISpot polyvinylidene difluoride (PVDF) 96-well plates (Merck Millipore, Darmstadt, Germany) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2021Quote: ... After a 10 min centrifugation step (15,000 x g) the samples were filtered in a 96-well MultiScreen filter plate (0.65 μm, Millipore, Burlington, MA). Reverse-phase liquid chromatography was performed using a DionexUltimate 3000 Series LC system (HPG-3400 RS High-Pressure pump ...
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bioRxiv - Microbiology 2020Quote: ... Pneumococcal isolates were grown in triplicate in BHI in 96-well plates in the presence of hydrogen peroxide (H202, Sigma) at concentrations 0 ...
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bioRxiv - Biochemistry 2021Quote: ... samples were taken from the reactions and the soluble fractions were separated from the insoluble substrate particles using a 96-well filter plate (Millipore) operated with a vacuum manifold ...
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bioRxiv - Molecular Biology 2021Quote: ... 2,000 cells were plated in a 96-well dish in DMEM media containing 10% FBS and selective antibiotics (1μg/ml puromycin (P8833, Sigma-Aldrich) and/or 5μg/ml blasticidin (71002-676 ...
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bioRxiv - Cell Biology 2020Quote: ... were seeded in DMEM supplemented with 10 % FBS in 96 well plates incubated with increased concentrations of compound 2 and treated with 3-NP at 10 mM (#N5636, Sigma). Then ...
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bioRxiv - Cell Biology 2021Quote: ... in polystyrene 96-well cell culture plates coated in 0.1% sterile gelatin (ES-006-B, Sigma Aldrich, St. Louis, MO). Approximately 50,000 cells were seeded for dense plating and 6,250 cells for sparse plating (1:8) ...
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bioRxiv - Bioengineering 2022Quote: ... HeLa cells were seeded at 25% confluency in 96 well plates and transduced with virus (lentiCRISPR-v2 with or without HPRT1 guide) and 8 μg/mL polybrene (Millipore). Virus was removed the next day and 2.5 μg/mL puromycin was added to remove cells that did not receive virus two days later ...
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bioRxiv - Biochemistry 2022Quote: ... Then 96 individual CFUs per biological replicate were resuspended in 100 µl of liquid YPD supplemented with 30% glycerol in a 96 well plate (NuncTM Sigma), as one colony/well ...
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bioRxiv - Microbiology 2020Quote: ... individual positive cells were sorted into the inner wells of a 96-well plate with 20 μl of a pre-reverse transcription (RT) lysis mix containing 0.1 μl NP40 (Sigma-Aldrich), 0.5 μl RNAse Inhibitor (Thermo Fisher) ...
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bioRxiv - Microbiology 2021Quote: HEK-ACE2 cells were seeded in a 96-well cell culture dish pre-coated with 0.1mg/mL poly-L-lysine (P9155-5MG, Sigma-Aldrich) and 24hr later ...
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bioRxiv - Neuroscience 2019Quote: Astrocytes were plated on PO/Lam coated 96 well plates and treated with 3 ng/mL IL-1α (Sigma; 3901), 30 ng/mL tumor necrosis factor alpha (R&D Systems ...
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bioRxiv - Microbiology 2019Quote: ... The same protocol was used when the fluorescence reporter was substituted by a luminescence readout in this case we utilize 96 well white cell-culture plates and the Firefly Luciferase Assay kit from Sigma following the manufacturer protocol.
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bioRxiv - Cell Biology 2019Quote: ... the cells were split to a 96-well plate in the same media and subsequently treated with (-/+) 10 nM water-soluble 17BE2 (Sigma) for 24 hours ...
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bioRxiv - Immunology 2021Quote: ... single-cell suspensions were prepared from spleens of mice and plated in Millipore MultiScreen-HA 96-well filter plates (Millipore) pre-coated with anti-mouse IL-2 ...
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bioRxiv - Microbiology 2021Quote: ... Sensitivity to micafungin was measured at least in triplicate in a polymerization assay using a 96-well multiscreen high-throughput screen filtration system (Millipore) with a final volume of 100 µl ...
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bioRxiv - Immunology 2021Quote: Panning was performed against monomeric NTD directly absorbed to polystyrene plates (Maxisorb 96-well microtiter plates; Nunc, Sigma-Aldrich, USA). Blocking of plates and phages was conducted for 60 min using two blocking solutions ...
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bioRxiv - Immunology 2021Quote: The S-variant and RBD coupled bead sets were incubated with sera from immunized mice at 4 dilutions (1:100; 1:1000; 1:10000; 1:100000) for 1 h in 96-well filter plates (MultiScreenHTS; Millipore) and analyzed on a Luminex analyzer (Luminex / R&D Systems ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were plated onto the 96-well plates coated with poly-L-ornithine (Sigma-Aldrich, Merck KGaA, St. Louis, MO). Equal volumes of cell suspension were plated onto the center of the dish ...
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bioRxiv - Microbiology 2022Quote: ... Cytokine and chemokine levels were measured with a commercial rat cytokine/chemokine magnetic bead panel 96-well plate assay kit (Milliplex MAP kit, Merck Millipore), which detects 5 cytokines and chemokines including IP-10/CXCL10 ...
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bioRxiv - Developmental Biology 2022Quote: ... PreME cells were trypsinized into single cells and harvested into Corning Costar Ultra-Low attachment multiwell 96-well plate (Sigma) at 4,000 cells per well in hPGCLC induction medium ...
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bioRxiv - Neuroscience 2022Quote: Cell survival was measured by tile imaging direct fluorescence of the VAChT-tdTomato reporter in iCrMNs and iSpMNs differentiated in a 96 well plate and treated with cyclopiazonic acid (CPA, C1530-10MG, Sigma) on differentiation day 10 ...
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bioRxiv - Physiology 2022Quote: HUVEC cultured in 96-well black plates were incubated in a 25 μM solution of 2’7’-dichlorodihydrofluorescein diacetate (DCFDA, Sigma, D6883) at 37°C for 30 min ...
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bioRxiv - Molecular Biology 2024Quote: ... The aggregates were removed from the supernatant using a 0.45 µm pore size 96 well filter plate (Millipore, cat#MSHVN4550). The flow-through was transferred into a new plate ...
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bioRxiv - Developmental Biology 2024Quote: ... animals were transferred to a 96-well plate in a container filled with silica gel beads (Sigma-Aldrich, MO, USA) to produce a low-humidity environment (approx ...
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bioRxiv - Immunology 2024Quote: ELISpot assays for OVA-specific IgE and IgG1 antibody-secreting cells (ASCs) were performed using Multiscreen white 96-well filter plates with Immobilon-P PVDF membranes (Millipore). Plates were prepared by pre-wetting with 30 µL of 35% ethanol in water for 1 minute ...