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Citations for Millipore Sigma :
1201 - 1250 of 1261 citations for Atosiban Acetate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: wtKSHV inoculum was obtained from BCBL-1 cells (5×105 cells/ml) induced with 20 ng of TPA (12-O-tetradecanoylphorbol-13-acetate; Sigma, St.Louis, USA)/ml ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... cells were seeded at a density of 1x105 cells/ml in 96 well plates and differentiated for 48 hours with 25 nm PMA (phorbol-12-myristate-13-acetate, Sigma-Aldrich, #P1585). Cells were then left to rest for 24 hours in fresh media containing no PMA prior to bleomycin exposures.
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bioRxiv - Neuroscience 2023Quote: ... Cells were plated on 6-well cell culture-treated plates and differentiated using 100 nM phorbol 12-myristate 13-acetate (PMA) (Sigma-Aldrich, P1585) for 72 h in serum-free RPMI 1640 medium (SFM ...
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bioRxiv - Plant Biology 2022Quote: ... Relative quantification of all the VOCs was done by normalizing the peak area of the analytes with that of the internal standard nonyl acetate (Sigma-Aldrich, India).
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bioRxiv - Molecular Biology 2022Quote: ... Stimulation of Jurkat cells for 2-7 hour experiments was achieved with 50ng/ml Phorbol 12-myristate 13-acetate (PMA, Sigma-Alrich, P8139) and 500ng/ml ionomycin calcium salt from Streptomyces conglobatus (ionomycin ...
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bioRxiv - Microbiology 2023Quote: ... Corresponding water samples were taken by filtering 2-L of tank water through 0.22-μm pore-size cellulose acetate membranes (Millipore Sigma, Burlington, MA). Samples were stored at −80°C until processing.
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bioRxiv - Molecular Biology 2024Quote: ... VFLIP for narrow window selection was buffer exchanged into 200 mM ammonium acetate using 50 kDa molecular weight cut-off (MWCO) Amicon® Ultra Centrifugal Filter (Sigma Aldrich). Samples were run at approximately 1 µM complex concentration for native mass spectrometry ...
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bioRxiv - Microbiology 2024Quote: ... cell differentiation into macrophages was induced by adding 50 nM phorbol 12-myristate 13-acetate (PMA) (Sigma-Aldrich, St. Louis, MO, USA) to the medium for 72 hours.
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bioRxiv - Microbiology 2023Quote: ... Jurkat Timer clones were treated for 24 h with a combination of T-cell stimulants: phorbol 12-myristate 13-acetate (SIGMA, Cat# P1585) at a final concentration of 20 ng/ml and ionomycin (Nacalai Tesque ...
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bioRxiv - Immunology 2023Quote: ... the cells were incubated in complete RPMI media containing 200 ng/mL of Phorbol 12-myristate 13-acetate (PMA) (Sigma-Aldrich, #P8139) for 48 hours ...
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bioRxiv - Genomics 2022Quote: ... The total culture was then extracted through rigorous shaking with an equal volume of ethyl acetate (Sigma-Aldrich, St. Louis, MO, USA). The organic layer was removed and dried over anhydrous MgSO4 (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2023Quote: ... TC-HSMAM1 and Cas9-TC-HSMAM1 cells were maintained in a complete RPMI-1640 medium and 100 ng/mL of phorbol 12-myristate-13-acetate (PMA) (Sigma-Aldrich, USA) at 37°C for 48 h to induce cell differentiation to macrophages 31 ...
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bioRxiv - Immunology 2024Quote: ... at a concentration of 1-2 × 106 cells/mL in a final volume of 20 mL and treated with either 100 nM of phorbol 12-myristate 13-acetate (PMA) (Sigma Cat#P1585), or an equivalent volume of Dimethyl sulfoxide (DMSO ...
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bioRxiv - Microbiology 2020Quote: Proteolysis was performed using 2 μM of encapsulated or unencapsulated DypB in proteolysis buffer (100mM Sodium acetate pH 5, 2mM DTT) with or without the addition of 1 μM Cathepsin B (Sigma-Aldrich Cat# 219362). Reactions were run for 14 hours at 40°C followed by analysis of samples by SDS-PAGE using 4-20% Criterion™ polyacrylamide gels (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: Total RNA was isolated from the throat swabs collections stored as frozen PBS suspensions at −20°C using a manual TRI Reagent – chloroform extraction and sodium acetate – ethanol precipitation (Sigma-Aldrich, Poznań, Poland). Presence of SARS-CoV-2 material in the collected sample was tested using GeneFinder real-time COVID-19 plus kit (OSANG Healthcare ...
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bioRxiv - Plant Biology 2021Quote: ... medium, containing 3% sucrose, 10−6 M potassium indole acetate (Nacalai Tesque, Kyoto, Japan) and 10−5 M kinetin (Sigma, St. Louis, MO) at 25 °C ...
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bioRxiv - Neuroscience 2022Quote: ... we used 10 μl amyl acetate (AM, Sigma-Aldrich, Cat. No: STBF2370V, diluted 1:50 in paraffin oil-Sigma-Aldrich, Cat. No: SZBF140V) and octanol (OCT ...
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bioRxiv - Microbiology 2022Quote: ... The peptide mix was then reconstituted in 50 mM sodium acetate pH 5 and treated with 0.25 U/mL Clostridium perfringens neuraminidase (Sigma-Aldrich Cat. Nr. N3001) at 37 °C for 3 hours ...
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bioRxiv - Microbiology 2019Quote: Macrophage-like phenotype was obtained by treating cells with 25 ng/mL phorbol 12-myristate 13-acetate (PMA, Sigma Aldrich, St-Louis, USA) for 48 h in 24-wells culture plates (Corning ...
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bioRxiv - Biophysics 2019Quote: A final concentration of 20 µM AS (WT or mutant) was obtained in 20 mM ammonium acetate (Sigma Aldrich, St. Louis, MO, USA) pH 7 and measured as a control ...
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bioRxiv - Microbiology 2019Quote: ... and the pellet was resuspended in 0.1 M sodium acetate buffer (pH. 6) and dialysed against four changes of buffer using Amicon ultra centrifugal filters (Merck, Millipore, Tullagreen, Carrigtwohill, IRL). The retentate was recovered and incubated with 100 µl of Micrococcus luteus peptidoglycan (1 mg/ml ...
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bioRxiv - Developmental Biology 2020Quote: ... The digested product was analysed on a 2% agarose gel in 1X Tris-Acetate EDTA (TAE) buffer (Sigma-Aldrich, Saint Louis, MO, USA) to highlight wt or lakritz mutant corresponding fragments ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Retinoic acid (RA) and 12-O-tetradecanoylphorbol-13-acetate (TPA) used for cell differentiation were purchased from Sigma-Aldrich (R2625 and P8139, respectively). According to our previous work (Carmona et al. ...
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bioRxiv - Biochemistry 2020Quote: ... U937 and THP-1 cells were pre-stimulated with 100ng/ml Phorbol 12-myristate 13-acetate (PMA) (Sigma-Aldrich, St. Louis, MO, USA) for 1h in RPMI-1640 medium containing 1% FBS.
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bioRxiv - Physiology 2021Quote: Muscle fibers were impaled with 2 sharp microelectrodes filled with 2 M potassium acetate solution containing 1 mM sulforhodamine 101 (Sigma-Aldrich, Catalogue #S7635) to allow for visualization ...
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bioRxiv - Immunology 2020Quote: ... Sample separation was performed using a C8 column and solvent system consisting of ammonium acetate (5 mM, Sigma Aldrich, St Louis, MO, USA) combined with acetic acid (0.1% ...
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bioRxiv - Immunology 2020Quote: Equine PBMCs (4 × 106 cells) were cultivated with 20 ng/mL of phorbol 12-myristate acetate (PMA; Sigma–Aldrich, St. Louis, MO, USA) and 1 μg/mL of ionomycin (Sigma–Aldrich ...
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bioRxiv - Microbiology 2021Quote: ... Cells (~106/ well) were seeded into wells of a 24-well dish and activated with 25 nM phorbol myristate acetate (PMA) (Sigma-Aldrich, MO, USA) for 10 minutes ...
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bioRxiv - Immunology 2022Quote: ... dispersed cells were cultured for 4 h in RPMI/10% fetal calf serum (FCS) with 1.5 ng/ml phorbol 12-myristate 13-acetate (PMA) and 1 μg/ml ionomycin (all from Sigma-Aldrich, St Louis, Mo) with Golgi-Stop (BD Bioscience ...
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bioRxiv - Neuroscience 2022Quote: ... cells were first stimulated with phorbol myristate acetate (PMA, 50ng/mL, Fisher BioReagents, cat. #50-058-20001) and ionomycin (750 ng/mL, Millipore Sigma, cat #AAJ62448MCR) in the presence of GolgiStop (1:1000 ...
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bioRxiv - Immunology 2022Quote: ... IFN-γ and TNF-α expression was measured by stimulating cells with 10 ng/ml phorbol 12-myristate 13-acetate (PMA) and 100 μg/ml ionomycin (both Sigma-Aldrich, München, Germany) for 4 hours in the presence of 5 μg/ml Brefeldin A (Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... Monocyte-derived macrophages were derived by treating the cell lines with 100 ng/ml of phorbol 12-myristate 13-acetate (PMA; Sigma, St. Louis, MO) for 48 h followed by a 24 h incubation in medium alone before experiments ...
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bioRxiv - Microbiology 2024Quote: ... 100 μl of serum was mixed with 100 μl of acetate buffer (0.2 mol/L, pH 5.0) containing 100 units of ß-glucuronidase/aryl sulfatase (Cat. No. 1041140002, Sigma-Aldrich, St. Louis, MO) and 50 μM of the internal standard 4-HBP (Cat ...
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bioRxiv - Immunology 2024Quote: ... U-937 cells (2×105 cells/mL) were incubated in the culture medium containing 200 ng/mL of phorbol myristate acetate (PMA) (Sigma-Aldrich; Merck KGaA) for 24 h to induce differentiation into macrophage cells ...
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bioRxiv - Microbiology 2024Quote: Cells were exposed to latency reversal agents as indicated with the following concentrations used: Phorbol 12-myristate 13-acetate (PMA, Sigma-Aldrich Cat# 79346) final concentration 50 ng/ml ...
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bioRxiv - Microbiology 2023Quote: ... the meshes were washed with three drops of distilled water and stained for one minute with uranyl acetate (Sigma, St Louis, MO, US) at a concentration of 1% ...
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bioRxiv - Biophysics 2022Quote: THP-1 cells were cultured in the cell culture media described before with 20nM phorbol 12- myristate 13-acetate (PMA) (Sigma Aldrich, Ref. P1585) for 48h in glass-bottom Petri dishes ...
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bioRxiv - Plant Biology 2023Quote: ... 1.5-ml glass vials (Ø×H 11.6 × 32 mm) containing ∼ 100 mg glass wool were filled with 200 µl (Z)-3-hexenyl acetate (HAC, >98%, Sigma-Aldrich, Buchs, Switzerland) and sealed with screw caps containing a rubber septum ...
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bioRxiv - Neuroscience 2024Quote: ... we used 10 µl amyl acetate (AM, Sigma-Aldrich, Cat. No: STBF2370V, diluted 1:50 in paraffin oil-Sigma-Aldrich, Cat. No: SZBF140V) and octanol (OCT ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... THP-1 derived macrophages were obtained by differentiating THP-1 cells with phorbol 12-myristate 13-acetate (PMA) (Sigma-Aldrich, St. Louis, MO, USA) treatment at 100 nM for 24 h according to the previous study (Li et al. ...
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bioRxiv - Immunology 2022Quote: ... The supernatant of both freshly sorted UTCs and UTC-derived clones after 24 hours of stimulation with phorbol myristate acetate (PMA, 1 ng/mL; Sigma-Aldrich, 16561-29-8) + ionomycin (0.5 μg/mL ...
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bioRxiv - Zoology 2019Quote: ... The response to pentyl acetate was significant compared to the DMSO control when working solutions were prepared from a 99% purity stock odor (Sigma-Aldrich, Cat. Nr. 133108), and a ≥99% analytical standard grade (AG ...
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bioRxiv - Microbiology 2019Quote: ... THP-1 cells were incubated for 48 – 72 h with 0.16 μg/ml phorbol-12-myristate-13-acetate (PMA, Sigma-Aldrich, St. Louis, MO, USA). Human HL cells 23 were maintained in RPMI 1640 (BioWhittaker ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: Dexamethasone (DEX, cat. #D4902), prednisolone (PRED, cat. #P6004), and medroxyprogesterone 17-acetate (MPA, cat. #M1629) were all purchased from Sigma-Aldrich (St. Louis, MO).
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bioRxiv - Cancer Biology 2020Quote: ... was prepared from 1 × 106 neutrophils cultured in 1 mL of D10 medium with or without phorbol 12-myristate 13-acetate (PMA; Sigma-Aldrich, now MilliporeSigma, P8139) for 4 h ...
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bioRxiv - Microbiology 2020Quote: ... THP-1 cells were adjusted to 5×105 viable cells/ml and placed into fresh medium with 100 ng/ml phorbol 12-myristate 13-acetate (PMA; Sigma-Aldrich, St. Louis, MO) to induce differentiation into a macrophage-like state ...
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bioRxiv - Immunology 2021Quote: ... for 4 hours in the presence or absence of Phorbol Myristate Acetate (PMA) and Ionomycine with Brefeldin A (all from Sigma Aldrich, St Louis, MO) and Golgi stop (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: Cells from draining popliteal lymph nodes (1 x 106) were stimulated for 4 h at 37 °C with PMA (phorbol 12-myristate 13-acetate, 10 ng/mL, Sigma-Aldrich, Darmstadt, He, Germany) and Ionomycin (10 ng/mL ...
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bioRxiv - Cell Biology 2023Quote: ... . Ultrathin sections placed on grids were stained with 1% aqueous uranyl acetate (Agar Scientific, Stansted, UK) and 3% lead citrate (Sigma Aldrich, St. Louis, USA), and observed at the FEI Morgagni 268D microscope.
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bioRxiv - Genomics 2023Quote: ... 5 ml of tagmentation wash buffer (TMG) was prepared consisting of a final concentration of 10 mM Tris Acetate pH 7.5 (Sigma 93352 and Sigma A6283, respectively), 5 mM MgAcetate (Sigma M5661) ...