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Citations for Millipore Sigma :
1151 - 1200 of 10000+ citations for Urotensin 2 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... Bound polypeptides were eluted stepwise with 250 μg/ml FLAG peptide (Sigma Aldrich) diluted in BC100 buffer ...
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bioRxiv - Molecular Biology 2023Quote: ... Elution buffer was prepared immediately before use by adding 3XFLAG peptide (Sigma F3290) to TBS to a final concentration of 150 ng/µL ...
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bioRxiv - Biochemistry 2023Quote: Sequences encoding for these peptides were cloned into the pET-41b vector (Novagen) using the restriction sites MfeI and SalI using standard methods and verified by sequencing.
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bioRxiv - Neuroscience 2023Quote: ... after which the interesting proteins were competitively eluted using 3XFLAG peptide (Sigma, F4799). The eluent was then incubated overnight at 4°C with Ni-NTA beads (Qiagen ...
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bioRxiv - Immunology 2023Quote: ... the peptides were cleaned using C18 ZipTip® (Merck Millipore, Massachusetts, United States). Samples were run on a Bruker timsTOF Pro mass spectrometer (Bruker Daltonics ...
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bioRxiv - Neuroscience 2023Quote: ... The peptides formed from the digestion were further purified by µC18 ZipTips (Millipore) and resuspended in 0.1% formic acid in HPLC water.
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bioRxiv - Immunology 2023Quote: PBS or HY peptides were emulsified in Complete Freund’s Adjuvant (Sigma, Lyon, France) at a 1:1 ratio ...
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bioRxiv - Biochemistry 2023Quote: All peptides were manually synthesized on Rink-amide ChemMatrix® resin (Sigma-Aldrich) using DIC/oxyma mediated coupling of 5x molar excess of common Fmoc-protected amino acids for one hour or 2x molar excess of Fmoc-Lys(Boc ...
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bioRxiv - Immunology 2023Quote: ... and precipitated proteins competitively eluted with Flag peptide (150 ng/µl; F4799, Sigma). Lysates were denatured in SDS-PAGE buffer (4x Lämmli buffer (Bio-Rad ...
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bioRxiv - Biochemistry 2023Quote: ... The peptides formed from the digestion were further purified by C18 ZipTips (Millipore) and analyzed by LC-MS/MS ...
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bioRxiv - Microbiology 2023Quote: ... Peptides were desalted and resuspended in 0.1% FA using C18 stage tips (Millipore). Digested samples (500 ng ...
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bioRxiv - Microbiology 2023Quote: ... 20 µg of tryptic peptides were loaded onto an Ascentis® (Sigma–Aldrich) ES-C18 column (2.1 mm × 100 mm ...
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bioRxiv - Molecular Biology 2023Quote: ... Elution was performed by incubation of the beads with 3xFLAG peptide (Sigma Aldrich) at 37 °C for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... and nucleosomes were eluted by incubating the beads with 3xFLAG peptide (Sigma Millipore) at a concentration of 500 ng/μL in Buffer D for 1 hour ...
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bioRxiv - Cancer Biology 2023Quote: ... and nucleosomes were eluted by incubating the beads with 3xFLAG peptide (Sigma Millipore) at a concentration of 500 ng/μL in Buffer D for 1 hour ...
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bioRxiv - Microbiology 2023Quote: ... Peptides were eluted with ABC buffer and then desalted with C18 tips (Millipore) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... Bound complexes were eluted by incubation with 500 µg/mL 3xFLAG peptide (Sigma) for 10 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Proteins were eluted with 5 ml of 1 mg/ml Flag-peptide (Sigma), followed by the addition of 20 ml of QA buffer (50mM TRIS pH 7.7 ...
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bioRxiv - Molecular Biology 2024Quote: ... and the immunopurified complexes were eluted with 3X FLAG peptide (Merck Millipore/Sigma). For the tandem IP experiment ...
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bioRxiv - Molecular Biology 2024Quote: ... and the immunopurified complexes were eluted with 3X FLAG peptide (Merck Millipore/Sigma). For the tandem IP experiment ...
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bioRxiv - Cancer Biology 2024Quote: ... Peptides were desalted and resuspended in 0.1% FA using C18 stage tips (Millipore) prior to acquisition.
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bioRxiv - Cell Biology 2022Quote: ... 2-hydroxy-4’-(2- hydroxyethoxy)-2-methylpropiophenone (I2959) (Sigma-Aldrich, USA), a photoinitiatior (0.5% w/v ...
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bioRxiv - Molecular Biology 2020Quote: ... 2% (v/v) 2-mercaptoethanol and 2 mM PMSF) (Sigma-Aldrich) and homogenize ...
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bioRxiv - Neuroscience 2020Quote: Animals were anesthetized with avertin (2, 2, 2-Tribromoethanol (Sigma-Aldrich), 125-250 mg/kg ...
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bioRxiv - Cell Biology 2022Quote: ... 2-Aminoethyl diphenylborinate (2-APB; Sigma), caffeine (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... 2-Deoxyglucose (2-DG, Sigma #D6134) was administered via IP injection at 0.5 mg/g bodyweight ...
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bioRxiv - Developmental Biology 2021Quote: ... followed by cysteine blocking for 10 minutes at room temperature using methyl methanethiosulfonate (MMTS, Sigma) at a final concentration of 10mM ...
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bioRxiv - Cell Biology 2020Quote: ... and treated with FITC anti-mouse IgG antibody (F0257, Sigma; 1:1000 in blocking buffer) overnight at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... and treated with FITC goat anti-mouse antibody (F0257, Sigma; 1:2000 in blocking buffer) overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... antibodies we diluted in blocking solution with 0.01% Tween®20 (Sigma, St. Louis, MO) using rat monoclonal anti-HA 1:500 (Millpore Sigma ...
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bioRxiv - Developmental Biology 2021Quote: ... Embryos were blocked for 30 minutes in PBT + 1x WBR (Western Blocking Reagent, Sigma, #11921673001) and then incubated overnight at 4°C with primary antibody (mouse anti-mNeonGreen [32F6] ...
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bioRxiv - Neuroscience 2021Quote: ... Samples were immersed in the blocking solution composed of 10% dimethyl sulfoxide (Sigma-Aldrich 276855), 1X casein buffer (Vector ...
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bioRxiv - Physiology 2019Quote: ... They were then washed three times in PBS before blocking in 3% BSA (A8806; Sigma) in PBS for 1 hour ...
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bioRxiv - Bioengineering 2019Quote: ... followed by blocking solution containing mouse anti-α-actinin antibody (1:300, A7811; Sigma-Aldrich) for 60 min ...
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bioRxiv - Systems Biology 2021Quote: ... Then cells were incubated in a blocking solution containing 10% bovine serum albumin (BSA, Sigma) and 0.01% Triton X-100 for 1h at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... in Tris-NaCl blocking buffer (1:5) or PBS containing 1 % BSA (Sigma Life Science) and 0.3 % Triton X 100 (Sigma Life Science) ...
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bioRxiv - Neuroscience 2021Quote: ... Blocking was performed for 1h at RT using 20% normal goat serum (S26, Sigma-Aldrich) in Tris-NaCl blocking buffer (TNB) ...
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bioRxiv - Neuroscience 2021Quote: ... Following blocking in 5% SureBlock reagent (LubioScience, Zurich, Switzerland) diluted in PBS-Tween20 (PBST, Sigma) for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... Ovaries were then blocked in 1X casein/PBSTX(0.1%) (stock: 10X casein blocking buffer, Sigma) for 30 min and incubated with rabbit anti-Egl primary antibody (kind gift from R ...
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bioRxiv - Neuroscience 2020Quote: ... and blocked for 30 minutes in IF Blocking Buffer (3% (w/v) BSA (Sigma A8412), 0.1 % (v/v ...
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bioRxiv - Biochemistry 2021Quote: ... PVDF membranes were incubated in 1X Casein blocking buffer (10X stock from Sigma-Aldrich, B6429) made up in TBS ...
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bioRxiv - Neuroscience 2020Quote: ... we incubated the sections in blocking solution (1X PBS with 0.1% Triton X-100 (Sigma) and 10% normal goat serum (NGS ...
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bioRxiv - Genetics 2021Quote: ... After blocking with 3% skim milk in PBS-T (PBS with 0.1% Tween20 (Sigma-Aldrich)) at room temperature for 45 min ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were blocked with the blocking buffer (3% bovine serum albumin [BSA, Sigma-Aldrich A3059] and 0.1% saponin [Sigma-Aldrich S4521] dissolved in DPBS ...
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bioRxiv - Bioengineering 2020Quote: ... Antagonistic blocking of the AChR was achieved via 1mL bolus of 200µM d-tubocurarine (Sigma) to 1mL of surrounding basal medium (MM) ...
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bioRxiv - Cell Biology 2021Quote: ... Blocking solution contained PBS supplemented with 10 % goat-serum (biowest) and 0.1 % Triton-X100 (Sigma). Primary antibodies were incubated for 2 hrs and secondary antibodies for 1 hr ...
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bioRxiv - Neuroscience 2022Quote: ... incubated with primary antibodies diluted in blocking solution (mouse anti-parvalbumin (1:800) from Millipore #MAB1572 ...
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bioRxiv - Biochemistry 2022Quote: ... Membranes were blocked for 1 hour in blocking solution comprising 5 % nonfat-dried milk (Sigma) and 0.0025 % sodium azide solubilized in TBS-T (150 mM NaCl ...
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bioRxiv - Biophysics 2022Quote: ... followed by blocking with 20 μL of 0.1 % Polyvinylpyrrolidone (Sigma-Aldrich, Cat #9003-39-8) in MB for 1 minute ...
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bioRxiv - Neuroscience 2021Quote: ... followed by a 30 min blocking step in PBS++ (PBS with 0.3% horse serum (Millipore) and 0.3% Triton-X100 (Sigma) ...