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Citations for Millipore Sigma :
1101 - 1150 of 10000+ citations for 5 tert butoxy 5 oxopentanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... After the last EtOH wash the coverslips were incubated with methanol containing 5% acetic acid and 1% (3-Aminopropyl)triethoxysilane (Millipore-Sigma) overnight in a vacuum desiccation chamber in the dark ...
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bioRxiv - Neuroscience 2024Quote: ... 0.5 µM (NMDA receptor antagonist that preferentially binds to GluN2C/GluN2D (40)) or DL-2-Amino-5-phosphonovaleric acid (APV, Sigma, A5282) 100 µM (broad spectrum NMDA receptor blocker).
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bioRxiv - Immunology 2019Quote: ... and placed in 1X PBS containing 5 mM EDTA and 5 mM DTT (Sigma-Aldrich). Tissues from 3-4 pups (per infection group ...
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bioRxiv - Microbiology 2020Quote: ... 5-10 ml of schizonts at <5% parasitaemia were lysed using 0.1% saponin/PBS (Sigma) for 10 min (room temperature) ...
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bioRxiv - Biochemistry 2021Quote: ... The reaction was quenched by addition of 5 μL 5% (v/v) hydroxylamine (Sigma Aldrich), followed by a 15 minutes incubation step ...
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bioRxiv - Molecular Biology 2022Quote: ... Oligonucleotides 5’-GAAAAAAAAAATATACGCTAAGATTTTTGG-3’ and 5’-ATGACTAAACCCCCCCTCC-3’ synthesized and HPLC-purified by Sigma-Aldrich were used ...
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bioRxiv - Cancer Biology 2019Quote: ... 5-chloro-2’-deoxyuridine (CIdU) and 5-lodo-2’-deoxyuridine (ldU) were obtained from Sigma.
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bioRxiv - Genetics 2021Quote: ... protease inhibitors (5 μM E64, 10 μM Bestatin, 5 μM Pepstatin, 48 hours (Merck Millipore)) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... injections were resuspended in a 5% EtOH /5% Kolliphor-EL (Sigma-Aldrich Cat. No. C5135) / 90% water for injections vehicle ...
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bioRxiv - Bioengineering 2023Quote: ... Tendons (n = 5/group/time) were then digested with proteinase-K (5 mg/ml) (Sigma) for 18 hours and stored at -20C until further assays could be performed ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and 5-fluorouracil (5-FU) and crystal violet from Sigma-Aldrich (St. Louis, MO, USA). They were dissolved in dimethylsulfoxide (DMSO ...
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bioRxiv - Neuroscience 2023Quote: Subcutaneous injections of 5-Chloro-2’-deoxyuridine (CldU) or 5-Iodo-2’deoxyurdine (IdU) (Sigma) were administered (50μg/g dissolved in saline ...
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bioRxiv - Cancer Biology 2024Quote: ... 5-diphenyl-2H-tetrazolium bromide solution (MTT) (Sigma-Aldrich, A2231, 5 mg/mL in PBS) to each well ...
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bioRxiv - Physiology 2024Quote: ... CDH1 Forward 5′-TTACTGCCCCCAGAGGATGA-3′ and Reverse 5′-TGCAACGTCGTTACGAGTCA-3′;) were purchased from Sigma-Aldrich. mRNA expression was determined using the comparative 2-ΔΔCt method and normalized to the mRNA expression level of endogenous references (PPIA ...
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bioRxiv - Neuroscience 2024Quote: ... 5 µl of the supernatant was incubated with 5 µg of amyloglucosidase (Sigma- Aldrich A1602) with 50 mM Na acetate for 30 minutes at 37 °C ...
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bioRxiv - Cancer Biology 2019Quote: ... Culture medium or ROS inducers such as tert-butyl hydroperoxide (Sigma-Aldrich), menadione (Enzo ...
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bioRxiv - Biochemistry 2020Quote: ... and subsequently with 10 μL tert-butyldimethylsilyl-N-methyltrifluoroacetamide (Sigma, Cat#: 394882) at 70°C for 90 minutes ...
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bioRxiv - Biochemistry 2021Quote: ... and subsequently with 10 μL tert-butyldimethylsilyl-N-methyltrifluoroacetamide (Sigma, Cat#: 394882) at 70°C for 60 minutes.
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bioRxiv - Neuroscience 2022Quote: Methyl-tert-butyl-ether (MTBE) and chloroform were purchased from Sigma Aldrich; methanol was from Fisher Science Education ...
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bioRxiv - Neuroscience 2020Quote: ... α-Tocopherol (50μM) and tert-Butyl hydroperoxide (25μM) (Sigma, St Louis, MO) were used as negative and positive controls and added for 2h to the cultures ...
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bioRxiv - Biochemistry 2021Quote: ... and subsequently with 10 μL tert-butyldimethylsilyl-N-methyltrifluoroacetamide (Sigma, Cat#: 394882) at 70°C for 90 minutes.
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bioRxiv - Cancer Biology 2022Quote: ... cells were treated with 50 µM tert-Butyl hydroperoxide (B2633, Sigma-Aldrich). Cells were harvested ...
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Dopamine depletion can be predicted by the aperiodic component of subthalamic local field potentialsbioRxiv - Neuroscience 2021Quote: ... 4 or 8 μl of 6-OHDA (5 μg in 1 μl saline containing 0.1% ascorbic acid; Tocris, Bristol, ENG and Sigma-Aldrich, MA, USA) was injected into the left MFB at the following coordinates ...
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bioRxiv - Molecular Biology 2022Quote: ... The reactions were quenched by adding 5 mM ethylene glycol-bis(2-aminoethylether)-N,N,N’,N’-tetraacetic acid (EGTA) (Sigma-Aldrich, E3889) and pelleted afterwards ...
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bioRxiv - Synthetic Biology 2020Quote: ... The strains were precultured on Yeast Nitrogen Base (YNB) medium without amino acids and with 5 g/L ammonium sulfate (Sigma-Aldrich Y0626) and 10 g/L glucose ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... We mixed the resulting peptides with a MALDI matrix consisting of an aqueous 50% acetonitrile/0.1% TFA solution of α-cyano-4-hydroxycinnamic acid (5 mg/ml; Sigma-Aldrich, www.sigmaaldrich.com). We measured mass spectra using an Ultraflex III MALDI-TOF (Bruker Daltonics ...
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bioRxiv - Immunology 2020Quote: ... and 50 μL supernatant was then mixed with equal parts Griess reagents A (dH2O with 1 % sulphanilamide [Sigma-Aldrich] and 5 % phosphoric acid [Sigma-Aldrich]). The mixture was incubated in the dark for 10 min at room temperature (RT) ...
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bioRxiv - Cancer Biology 2021Quote: ... lyophilized antigen derived from media conditioned by the LS180 colon cancer cell line(5) was deglycosylated using anhydrous trifluoromethanesulfonic acid (TFMS) per manufacturer (Sigma-Aldrich, USA) protocol ...
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bioRxiv - Genomics 2021Quote: ... cells were first washed once with phosphate-buffered saline (PBS) and supplemented with equilibrated (37 °C and 5% CO2) medium containing 500 μM indole-3-acetic acid (Sigma-Aldrich, I5148) freshly prepared before use ...
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bioRxiv - Biochemistry 2022Quote: ... The peptides were purified by reverse-phase HPLC (Acetonitrile in Water with 0.1% formic acid) using either a Discovery BIO wide pore C18-5 column (25 cm x 10 mm, Millipore-Sigma #568230-U) or a Shim-pack GIS C18 (20 × 250 mm ...
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bioRxiv - Biochemistry 2022Quote: ... The peptides were purified by reverse-phase HPLC (Acetonitrile in Water with 0.1% formic acid) using either a Discovery BIO wide pore C18-5 column (25 cm x 10 mm, Millipore-Sigma #568230-U) or a Shim-pack GIS C18 (20 × 250 mm ...
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bioRxiv - Bioengineering 2022Quote: ... cell-seeded wells (n=3 per group) were rinsed with PBS and incubated in 500 μL of 5% trichloroacetic acid (TCA, Sigma-Aldrich, T6399) in UPW for 30 minutes ...
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bioRxiv - Evolutionary Biology 2022Quote: ... The cultures were then induced in SGDCAA (6.7 g/L YNB without amino acid [VWR #90004-150], 5 g/L ammonium sulfate [Sigma-Aldrich #A4418], 2% galactose [Sigma-Aldrich #G0625] ...
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bioRxiv - Cell Biology 2023Quote: ... 200 μl of cell suspension was collected and mixed with 200 μl of ice-cold HBSS containing 5% fatty acid-free bovine serum albumin (BSA, Merck Millipore, 126575-10GM). To quench the fluorescence of non-internalized NBD-phospholipids ...
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bioRxiv - Systems Biology 2022Quote: Pooled tissue samples were separately dipped for 30 s at room temperature into 10 ml of chloroform containing 5 μg of heptadecanoic acid (Sigma, St. Louis) as the internal quantification standard ...
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bioRxiv - Plant Biology 2023Quote: ... 4-day old Col_0 seedlings were incubated with LRC liquid medium supplemented with indicated concentrations of compounds (Adenosine 5′-triphosphate magnesium salt, ATP-Mg [VWR]; Abscisic acid, ABA [Sigma-Aldrich]; Carbonyl cyanide 3-chlorophenylhydrazone, CCCP [Sigma-Aldrich] ...
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bioRxiv - Bioengineering 2023Quote: Cells were incubated in 5 mM ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid EGTA (Sigma-Aldrich, E3889) diluted in Endothelial Growth Medium at 37°C and 5% CO2 for 2 hours ...
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bioRxiv - Biochemistry 2023Quote: ... 2 µL of sample was mixed with 4 µL of TLC-MALDI buffer (MB, 5 mg/mL α-cyano-4-hydroxycinnamic acid (CHCA, Sigma-Aldrich), 25% acetonitrile ...
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bioRxiv - Synthetic Biology 2023Quote: ... 5 µl of the sample solution was mixed with 5 µl of a saturated solution of α-cyano-4-hydroxycinnamic acid matrix for MALDI-MS (Sigma-Aldrich) in acetonitrile ...
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Sequential dynein effectors regulate axonal autophagosome motility in a maturation-dependent pathwaybioRxiv - Cell Biology 2020Quote: ... The chamber was then blocked with two 5 minute incubations of 5% pluronic F-127 (Sigma). Labeled (labeling ratio of 1:40 ...
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bioRxiv - Neuroscience 2020Quote: ... sections were rinsed in SSC 5 X for 5 min then washed in formamide 50% (Sigma)-SSC 2 X for 30 min at 60°C ...
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bioRxiv - Cell Biology 2020Quote: ... washed with PBS for 5 min and blocked with 5% bovine serum albumin (BSA; Sigma-Aldrich) in PBS-Tween 20 (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... were performed with MTT (3-(4, 5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide) (Sigma-Aldrich) as described in Sanz et al ...
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bioRxiv - Molecular Biology 2019Quote: ... and 10 µl MetaPolyzyme (5 mg/ml) and 5 µl sodium azide (0.1 M, Sigma-Aldrich) was added to the dissolved pellet sample ...
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bioRxiv - Cancer Biology 2019Quote: ... 5-chloro-2′-deoxyuridine (CldU) and 5-Iodo-2′-deoxyuridine (IdU) were obtained from Sigma-Aldrich. Click-iT EdU Alexa Fluor 488 Imaging Kit was obtained from Invitrogen ...
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bioRxiv - Biochemistry 2021Quote: ... antipapain and pepstatin A) supplemented with 5 mM MgCl2 and 5 KU Benzonase nuclease (Merck Millipore). After incubation at 4°C for 30 minutes to assure the complete lysis ...
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bioRxiv - Biochemistry 2019Quote: ... 5-aminoimidazole-4-carboxamide-1-β-D-ribofuranosyl 5’-monophosphate (ZMP; Sigma Aldrich, St. Louis, MO) at a stock concentration of 50 mM in dimethyl sulfoxide (DMSO ...
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bioRxiv - Molecular Biology 2021Quote: ... 5 mM ZnCl2 and 50 mM NaCl) and 5 mU phosphodiesterase II from Sigma (# P9041-10UN) were added and the mixture was incubated at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Protein extracts were reduced with 5 mM 5-Tris (2-carboxyethyl) phosphine hydrochloride (TCEP) (Sigma-Aldrich) for 2 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... cultures were treated with 5 μM FDU (5-fluoro-2’-deox-yuridine, Sigma-Aldrich, MO, USA) to further reduce the number of glial cells ...