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Citations for Millipore Sigma :
1051 - 1100 of 2589 citations for Rat FAM24A shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Primary human epithelial cells seeded on 10 cm2 dishes were cultured in BEGM medium and transduced with lentiviral particles harboring shRNA against KLK13 (a set of 3) or GFP gene in the presence of polybrene (5 µg/ml; Sigma-Aldrich, Poland). Cells were further cultured for 72 h at 37°C with 5% CO2 and positively transduced cells were selected using puromycin (5 µg/ml Sigma-Aldrich ...
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bioRxiv - Microbiology 2024Quote: ... TRCN0000148694) and a control scrambled shRNA pLKO.1 clone (SHC-002) were performed using MISSION Lentiviral Packaging Mix (Sigma-Aldrich, Diegem, Belgium) and FuGENE HD (Promega ...
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bioRxiv - Cancer Biology 2023Quote: ... laboratory protocol “Lentivirus production of shRNA or ORF-pLX clones,” and single clones were isolated following puromycin selection (2 μg/mL; Sigma-Aldrich #P8833). RNA-seq data were aligned with STAR to mouse reference genome mm10 (GRCm38) ...
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bioRxiv - Cell Biology 2020Quote: ... Plasmid DNA was extracted from bacteria using GenElute™ Plasmid Miniprep Kit (Sigma Aldrich), following the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... we transfected lentiviral plasmids with packaging plasmids in 293T cells using polyethylenimine (Sigma, 408727), harvested supernatants containing viral particles 48 and 72 hours later ...
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bioRxiv - Cell Biology 2023Quote: ... Plasmids were prepared using the GenElute HP Endotoxin-Free Plasmid Maxiprep Kit (NA0410; Sigma). Silica fines in the final product were carefully eliminated by additional centrifugation at 12,000 g for 30 min.
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bioRxiv - Molecular Biology 2023Quote: ... Plasmids were extracted with the Gene Elute Plasmid Miniprep kit (Sigma, St. Louis, USA) and sent for sequencing to two different companies (EuroFins Genomics ...
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bioRxiv - Molecular Biology 2019Quote: ... The plasmid pET-28a (+) (Novagen) carries an N-terminal His-Tag/thrombin/T7 configuration ...
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bioRxiv - Cancer Biology 2020Quote: ... ShScr and shIDO plasmids (Sigma)[35] were electroporated into ST strains using a BTX electroporator (1mm gap cuvettes ...
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bioRxiv - Biochemistry 2020Quote: ... carrying the pRARE2 plasmid (Novagen) and initially purified using the same protocol ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... The pRSFDuet-1 plasmids (Novagen) encoding SARS-CoV-2 nsp14 or nsp10 engineered with an N-terminal His-SUMO tag were prepared as follows ...
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bioRxiv - Microbiology 2021Quote: ... plasmids and primers (Sigma, Singapore) used in this study are listed in S6 and S7 Tables ...
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bioRxiv - Synthetic Biology 2023Quote: ... The plasmid pET-19b (Novagen) was double digested using NcoI and BamHI and Gibson assembly performed to insert a gene construct into the plasmid ...
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bioRxiv - Synthetic Biology 2023Quote: The pET-11a plasmid (Novagen) was used as the genetic vector to host all three components constituting our genetic circuit ...
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bioRxiv - Cancer Biology 2022Quote: ... Plasmids were purchased from Sigma.
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bioRxiv - Genetics 2023Quote: ... harboring the pRARE plasmid (Novagen). Super Broth media (3 L ...
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bioRxiv - Immunology 2021Quote: ... Expression of PTSN was silenced in U2-OS and 293A cells by lentiviral transduction of a pLKO1 shRNA against both β- and α-PTSN (TRCN0000282572, Sigma-Aldrich). pLKO1 shRNA (SHC016V ...
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bioRxiv - Microbiology 2019Quote: ... The Isopropyl β-D-1-thiogalactopyranoside (IPTG)-inducible lentiviral backbone pLKO-puro-IPTG_3xLacO harboring the non-mammalian shRNA control was purchased (Mission™SHC332; Sigma Aldrich, Buchs, Switzerland). The puromycin resistance gene was replaced via restriction digestion cloning using a PCR-amplified fluorescent mCherry marker containing overhangs with compatible restriction sites to generate the pLKO-mCherry_IPTG_3xLacO lentiviral backbone.
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bioRxiv - Cancer Biology 2019Quote: ... for primary cells miR-E shRNA viruses were concentrated to increase transduction efficiency using Centriprep Centrifugal filter YM-50 (Millipore, Billerica, MA, USA).
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bioRxiv - Physiology 2021Quote: ... The shRNA-containing vector was co-transfected with the pE-ampho vector into HEK293T cells using GeneJuice transfection reagent (Merck Millipore, Burlington, MA, USA). Supernatants containing viral particles were collected 48 h after the transfection ...
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bioRxiv - Cancer Biology 2022Quote: ... HT-29/19A and HCT-116 cells were transduced with lentivirus expressing short-hairpin RNAs (shRNAs) targeting fascin1 (Sigma-Aldrich, St. Louis, MO). For the rescue experiments ...
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bioRxiv - Immunology 2024Quote: ... Two different Mission lentivirus-based plasmids of shRNAs (clone numbers TRCN0000123050 and TRCN0000436778) against human ZBP1 and the shcontrol vector TRC2 pLKO.5-puro nonmammalian shRNA (SHC202) were obtained from Sigma-Aldrich (Burlington, MA). 293T cells were cotransfected with the shRNA and packaging plasmids psPAX2 and pMD2 using Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Immunology 2022Quote: Lentiviral plasmids encoding shRNA for gene knockdown for PRDM1-L or open reading frame of overexpression for PRDM1-S and PRDM1-L were obtained from Sigma-Aldrich (MISSION shRNA) and Horizon Discovery Biosciences (Precision LentiORF) ...
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bioRxiv - Cell Biology 2023Quote: U2Os-AP2-GFP cells were transduced with lentiviral media expressing respective shRNAs in the presence of Polybren 8 μg/ml (Sigma-Aldrich, TR-1003) for 5 hours ...
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bioRxiv - Neuroscience 2023Quote: Knockdown of proteins was achieved through RNA interference either by CaCl2 transfection of gene-specific shRNA encoding vectors (pLKO.1; Sigma-Aldrich or shRNAmiR) on DIV 7 or by transduction of cells with lentiviral particles harboring the gene-specific shRNA on DIV 2 ...
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bioRxiv - Cancer Biology 2021Quote: ... anti-hemagglutinin (rat monoclonal, catalog #11867423001, Millipore-Sigma, MA, RRID: AB_390918), and a marker of mTOR activation ...
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bioRxiv - Cancer Biology 2021Quote: ... anti-hemagglutinin (rat monoclonal, catalog #11867423001, Millipore-Sigma, MA, RRID: AB_390918), and a marker of mTOR activation ...
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bioRxiv - Microbiology 2021Quote: ... using rat monoclonal anti-HA 1:500 (Millpore Sigma, Burlington, MA) and rabbit anti-neomycin phosphotransferase II 1:1000 (Millpore Sigma ...
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bioRxiv - Microbiology 2021Quote: ... The was rat monoclonal anti-HA (Millpore Sigma, Burlington, MA, USA) was used as primary antibody (1:500 ...
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bioRxiv - Microbiology 2021Quote: ... Samples were probed with rat anti-HA 3F10 (Sigma, 1:500) in 4% BSA/PBS ...
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bioRxiv - Neuroscience 2020Quote: ... Rabbit or rat anti-NCAM (Millipore, Cat#: ABN2181-100UG, 1:400), chicken anti-GFP (Abcam ...
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Efficient Suppression of Endogenous CFTR Nonsense Mutations Using Anticodon Engineered Transfer RNAsbioRxiv - Molecular Biology 2021Quote: ... pre-coated with Collagen from rat tail (Sigma-Aldrich, #C7661-25MG) 5-6 days before assay and maintained in normal cell culture media at 37 °C and 5% CO2 ...
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bioRxiv - Neuroscience 2019Quote: ... overnigh incubation anti-DAT (1:2000; rat monoclonal; Millipore Sigma MAB369) followed 1 h incubation with secondary antibody (goat anti-rat HRP ...
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bioRxiv - Cell Biology 2021Quote: ... Coverslips were pre-coated with rat-tail collagen type I (Sigma) at 50 µg/mL in PBS at 25°C for 1 h ...
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bioRxiv - Neuroscience 2021Quote: ... Rats were trained with 0.2% saccharin (sodium salt hydrate, Sigma #S1002) in both bottles until they reached the criteria of >1000 licks across the session ...
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bioRxiv - Neuroscience 2020Quote: ... rats received an intraperitoneal injection of lithium chloride (LiCl; Sigma Aldrich; 25 mg/kg ...
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bioRxiv - Neuroscience 2020Quote: ... Primary antibodies used: rat anti-NFM-H (Millipore, MAB5448, 1:500), rabbit anti-NG2 (Millipore ...
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bioRxiv - Genetics 2020Quote: ... coated with 1% rat tail collagen (Millipore Sigma; Cat# 08-115). When cells reached 90% confluency ...
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bioRxiv - Neuroscience 2020Quote: Rats were deeply anesthetized with urethane (1.5 g/kg, Sigma-Aldrich, Germany ...
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bioRxiv - Cell Biology 2020Quote: ... rat anti-Collapsin Response-Mediated Protein 5 (CRMP5, 1:50, Millipore), mouse anti-γ-tubulin (1:50 ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Rat platelet samples were homogenized in TRI Reagent (Sigma-Aldrich, USA), then RNA from each sample was transcribed to complementary DNA by High Capacity cDNA Reverse Transcription Kit (Applied Biosystems ...
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bioRxiv - Cell Biology 2022Quote: ... rat anti-tubulin clone YL1/2 1:1500 (Millipore Sigma #MAB1864), rabbit anti-KIF22 1:500 (GeneTex #GTX112357) ...
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bioRxiv - Neuroscience 2022Quote: ... and rat anti-MBP (1: 1,000, MAB386, RRID: AB_94975, Millipore, Germany). The blots were rinsed in TBS-T and then incubated with horseradish peroxidase-conjugated anti-mouse antibody (1:4,000 ...
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bioRxiv - Neuroscience 2020Quote: ... then incubated with rat anti-GR (1:1000, Millipore-Sigma MABN778) and either rabbit anti-H4R3me2a (1:500 ...
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bioRxiv - Neuroscience 2021Quote: ... rat anti-Dopamine Transporter Antibody (DAT, 1:200, Millipore Sigma, #MAB369), rabbit anti-Tyrosine Hydroxylase (TH ...
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bioRxiv - Neuroscience 2022Quote: ... 1:1000), polyclonal rat anti-dopamine transporter (MAB369, Merck (Sigma-Aldrich); 1:1000).
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bioRxiv - Microbiology 2022Quote: ... Samples were probed with rat anti-HA 3F10 (Sigma, 1:1,000) in blocking buffer ...
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bioRxiv - Neuroscience 2022Quote: ... and rat anti-somatostatin primary antibody (1:1000, Millipore Sigma, MAB354) diluted in PBS containing 0.5% Triton X-100 and 2% NHS ...
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bioRxiv - Immunology 2022Quote: ... rat anti-PNAd monoclonal (clone: MECA-79, 1:100, MABF2050, Sigma). All secondary antibodies were purchased from ThermoFisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Rat-tail collagen was obtained from Sigma Aldrich (St Louis, MO). All other cell culture components such as fetal bovine serum (FBS) ...