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Citations for Millipore Sigma :
901 - 950 of 10000+ citations for Recombinant Mouse lgfbp4 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: ... the beads were incubated in 10 μg/ml recombinant E-cadherin (human, Sigma) solution overnight at 4 °C with agitation ...
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bioRxiv - Neuroscience 2023Quote: ... Michigan) and 0.6 TIU/mL of recombinant aprotinin was added (Sigma; Burlington, MA). For saliva ...
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bioRxiv - Cell Biology 2023Quote: ... 200 ng/mL recombinant human IGF-1 (LONG R3-IGF-1; Sigma-Aldrich), and 20 ng/mL human bFGF (Life Technologies)] for 14 to 35 days.
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bioRxiv - Plant Biology 2023Quote: ... The over-expressed recombinant proteins were then coupled to CNBr-activated Sepharose (Sigma) at a ratio of 2.5 mg protein to 1 ml Sepharose slurry as described by the manufacturers.
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bioRxiv - Biophysics 2023Quote: BCL-2 active humans (recombinant, expressed in E. coli) were purchased from Sigma. Navitoclax ...
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bioRxiv - Biochemistry 2023Quote: ... periplasmic expression of the recombinant GFP-nanobody in BL21(DE3) Rosetta cells (Novagen) was induced with 0.1 mM IPTG for 16 hours at 16°C ...
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bioRxiv - Biophysics 2023Quote: ... recombinant Rad26 protein was expressed in Escherichia coli strain Rosetta 2(DE3) (Novagen) and purified by Ni-NTA agarose (Qiagen) ...
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bioRxiv - Bioengineering 2023Quote: Insulin-azide and insulin-alkyne were prepared from recombinant human insulin (EMD Millipore) by selective acylation of the B29Lys residue as previously reported.33 ...
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bioRxiv - Bioengineering 2024Quote: ... Cell culture medium was supplemented with 1.5 μg mL−1 recombinant ApoE (Sigma), prepared according to manufacturer’s protocol ...
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bioRxiv - Bioengineering 2024Quote: ... and 100 U/mL premium grade recombinant human IL-2 (Sigma catalog #I7908) with media changes every 2-3 days to maintain the cells at a concentration of 0.2 M cells/mL.
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bioRxiv - Molecular Biology 2024Quote: ... into PBS with 0.05% BSA and 5 µL/mL recombinant RNAse inhibitor (Sigma). Isolation of single cells was verified with a haemocytometer ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant pH was adjusted to 8.0 and incubated with Ni2+-NTA His-binding resin (Novagen) that was pre-equilibrated with binding buffer (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... The Trx-His(6) tag was cleaved by addition of 300 units of thrombin (Sigma T4648) directly to the eluate and the solution dialyzed (Slide-A-Lyzer dialysis cassettes ...
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bioRxiv - Plant Biology 2020Quote: ... The His6-SEC5B fusion protein was extracted and purified with Ni-NTA His bind resin (Novagen). The freshly extracted resin-bound GST or GST-XIK proteins were mixed with purified His6-SEC5B protein in protein interaction buffer and incubated at 4°C for 2 h with shaking ...
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bioRxiv - Biophysics 2020Quote: ... MBP and the His-tag were cleaved from the P domain using HRV 3C protease (Novagen). Cleaved P domain protein eluted from Ni-NTA resin and was further purified by size-exclusion chromatography using a Superdex 16/600 200 pg column (GE Healthcare ...
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bioRxiv - Neuroscience 2019Quote: ... or combined together (GPA2-FLAG/GPB5-His) and then treated with DSS (Sigma Aldrich, Oakville, ON). In some experiments ...
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bioRxiv - Plant Biology 2021Quote: ... and GST were fused with a C-terminal octa-His tag in the pET42 vector (Novagen).
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bioRxiv - Molecular Biology 2022Quote: ... Cleared supernatant was incubated with 20 μl His Mag Sepharose Ni beads (Sigma, GE28-9673-88) overnight ...
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bioRxiv - Physiology 2022Quote: ... The F4-2_Fab protein was first purified using cOmplete® His-tag purification resin (Sigma-Aldrich), and L6-F4-2 protein was first purified with CaptivA Protein A affinity resin (Repligen) ...
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bioRxiv - Neuroscience 2024Quote: ... and the supernatant subjected to Ni-NTA affinity purification (HIS-Select Nickel Affinity Gel (Sigma Aldrich)) ...
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bioRxiv - Biochemistry 2024Quote: ... The UbVs and Ube2d2S22R-Avi were purified by nickel-affinity chromatography with HIS-select resin (Sigma) followed by an overnight 3C digestion using in-house produced 3C protease ...
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bioRxiv - Biochemistry 2024Quote: ... and the protein solution was mixed with Co resin (His-select Cobalt Affinity Gel, Sigma Aldrich) for 1h at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified by centrifugation and applied to cOmplete His-tag purification resin (Sigma Aldrich) for 1 hour at 4 °C with rotation ...
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bioRxiv - Molecular Biology 2024Quote: ... Clarified lysate was applied to 2.5 ml bed volume His-select Cobalt Affinity Gel (Sigma-Aldrich). The slurry was rotated in the cold room for an hour prior to application to a gravity flow column ...
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bioRxiv - Molecular Biology 2021Quote: ... SHH-tagged Gus-Cts1 was detected using a primary anti-HA antibody (1:4000, Millipore/Sigma, Billerica, USA). For detection of Gfp-tagged proteins like Don3-Gfp ...
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bioRxiv - Molecular Biology 2021Quote: ... SHH-tagged Gus-Cts1 was detected using a primary anti-HA antibody (1:4000, Millipore/Sigma, Billerica, USA). For detection of Gfp-tagged proteins like Don3-Gfp ...
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bioRxiv - Molecular Biology 2021Quote: ... Degradation of AID-tagged CHD4 was induced by the addition of 500 μM 3-Indoleacetic acid (I2886, Sigma).
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bioRxiv - Molecular Biology 2021Quote: ... StrepII-tagged proteins (RBBP6, symplekin and WDR33) were detected using streptavidin-HRP conjugate (Merck Millipore, cat. No. 18152) and Amershan ECL Detection Reagents (Cytiva ...
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bioRxiv - Microbiology 2019Quote: ... PTP-tagged proteins were detected by incubating with anti-protein A serum (1:3000, 1 h) (Sigma, P3775) followed by Alexa Fluor® 594 goat anti-rabbit (1:250 ...
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bioRxiv - Microbiology 2021Quote: ... For elution of FLAG-tagged proteins and their complexes 100 µL of 0.2 mg/mL FLAG3 peptide (Sigma) was added to samples ...
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bioRxiv - Microbiology 2021Quote: ... The expression of S-tagged NrdA was confirmed by purification using S-protein agarose (Merck Millipore, Darmstadt, Germany) as previously described (11 ...
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bioRxiv - Evolutionary Biology 2020Quote: ... F2 FLAG-tagged TBP::NveRLR::mCherry and wild-type zygotes were treated with 3% L-Cysteine (Merck Millipore), washed and snap frozen in liquid nitrogen ...
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Increased ACTL6A Occupancy Within mSWI/SNF Chromatin Remodelers Drives Human Squamous Cell CarcinomabioRxiv - Molecular Biology 2021Quote: ... The GST-tagged proteins were eluted by 10mM reduced glutathione (Sigma, 100mM stock made in 50mM Tris, pH7.6) in PBS (containing 1mM PMSF and 0.02% (∼3mM ...
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bioRxiv - Microbiology 2020Quote: ... Myc-tagged protein was eluted at room temperature with 25 μl myc-peptide (0.5mg/mL in PBS, Sigma) for 15 minutes at 700 rpm mixing ...
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bioRxiv - Biophysics 2023Quote: ... Lysate containing FLAG-tagged target protein was purified using ANTI-FLAG M2 Affinity Gel resin (Sigma-Aldrich, USA) and eluted with 3x FLAG peptide (APExBIO ...
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bioRxiv - Cell Biology 2023Quote: 75 μg GST-tagged Rab GTPase was loaded with either 10 mM GDP or GTP (Sigma Aldrich, Germany) in the presence of 20 mM EDTA and 50 mM HEPES-NaOH ...
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bioRxiv - Molecular Biology 2023Quote: ... and subjected to binding of FLAG-tagged peptide species with anti-FLAG M2 magnetic beads (Sigma, cat # M8823). The purified FLAG-tagged peptides were harvested by eluting from the anti-FLAG M2 magnetic beads with 100 ng/µl 3xFLAG peptides.
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bioRxiv - Biochemistry 2023Quote: ... GST-tagged protein was eluted off the beads by using 20 mM glutathione solution (Sigma-Aldrich, #G4251-5G). Fractions were analyzed by resolving on 4-20 % gradient SDS-PAGE (BioRAD #4561094 or #4651093) ...
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bioRxiv - Cell Biology 2023Quote: ... Immunoprecipitation of Flag-tagged proteins was performed with Anti-Flag M2 Affinity Gel (Sigma-Aldrich, cat.no. A2220-1ML) at 4 °C with overhead rotation for 6 h ...
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bioRxiv - Developmental Biology 2022Quote: ... Cy3-mouse-anti-mouse aSMA (Sigma C6198), mouse anti-mouse αSMA (Sigma A2547) ...
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bioRxiv - Molecular Biology 2019Quote: ... and 3×FLAG::GFP-tagged protein and associated RNAs were eluted with 100 μg/mL 3×FLAG peptide (Sigma). The eluates were incubated with TRIzol reagent (Invitrogen) ...
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bioRxiv - Cell Biology 2020Quote: Seeds of the relevant GFP-tagged lines were submerged in either glycerin or in solutions of NaCl (Sigma-Aldrich), mannitol (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: ... FLAG-tagged protein was detected by an anti-FLAG M2 antibody conjugated with horseradish peroxidase (A8592, Sigma-Aldrich, U.S.A.). CaMKIIβ and α-Tublin were detected by primary antibodies anti-CaMK2β (#139800 ...
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bioRxiv - Molecular Biology 2021Quote: ... Bound FLAG-HA-tagged KAT6B and associated proteins were eluted with 0.3 μg/μl 3x FLAG tag peptide (Sigma) in BC100 buffer for 1 h with gentle rotation at 4°C.
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bioRxiv - Synthetic Biology 2019Quote: FLAG-tagged encapsulins were purified from HEK293T cells using the ANTI-FLAG M2 Affinity Gel (SIGMA A2220, Sigma-Aldrich) in a batch format according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2019Quote: FLAG-tagged encapsulins were purified from HEK293T cells using the ANTI-FLAG M2 Affinity Gel (SIGMA A2220, Sigma-Aldrich) in a batch format according to the manufacturer’s protocol ...
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bioRxiv - Systems Biology 2020Quote: Recombinant HA-tagged Gln4 protein for use as a standard was produced by transforming E.coli strain Rosetta-gami B (DE3; Novagen) with pET19b-GLN4 ...
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bioRxiv - Microbiology 2021Quote: ... Endogenous HA-FLAG-tagged PPS parasite samples were probed with Roche rat anti-HA monoclonal 3F10 primary (Sigma 11867423001) and donkey-anti-rat DyLight800 (Invitrogen Life Technologies SA5-10032 ...
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bioRxiv - Cell Biology 2021Quote: Expression of Flag- and EGFP-tagged proteins was induced with media containing 1 µg/mL doxycycline (Sigma-Aldrich D9891) 12-24 hours prior to harvesting ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates containing FLAG-tagged motors were diluted in assay buffer [AB: P12 buffer (12 mM PIPES (Sigma P-1851), 1 mM EGTA (Sigma E-4378) ...