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Citations for Millipore Sigma :
901 - 950 of 10000+ citations for Folate receptor alpha FOLR1 Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... in-frame of the C-terminal (His)6-tag (Novagen, http://www.emdbiosciences.com) resulting in pET28a:ApTPS1 ...
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bioRxiv - Microbiology 2024Quote: ... with 4g/L Ox-bile (Sigma, or 2 g/L Hi-Media), 4 mL/L Tween60 (Sigma) ...
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bioRxiv - Cell Biology 2024Quote: ... another membrane was incubated with anti-His antibody (05-949, Millipore, USA) at 4°C for 12 h and then incubated with an IRDye 800 secondary antibody for 1 h ...
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bioRxiv - Plant Biology 2019Quote: ... Alpha-solanine (≥95% purity) and tomatidine (≥95% purity) were purchased from Sigma Aldrich (St. Louis, MO). Stock solutions were prepared by weighing each analyte into glass vials and dissolving into methanol prior to storage at −80 °C ...
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bioRxiv - Plant Biology 2021Quote: ... Alpha-solanine (≥95% purity) and tomatidine (≥95% purity) were purchased from Sigma Aldrich (St. Louis, MO).
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bioRxiv - Neuroscience 2020Quote: ... The western blot was also stained with an alpha-tubulin antibody (Sigma-Aldrich Cat# T9026, RRID:AB_477593) to ensure similar overall protein levels between conditions ...
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bioRxiv - Biochemistry 2022Quote: Western blots were performed using the following antibodies: anti-alpha-Tubulin (monoclonal; 1:2000; Sigma, T9026), rabbit anti-H3 (polyclonal ...
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bioRxiv - Microbiology 2023Quote: ... Gametocytes and microgametes were visualised by labelling with an anti-alpha tubulin antibody (clone DM1A (Sigma)) 1:500 dilution in blocking buffer for 1 h ...
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bioRxiv - Neuroscience 2021Quote: The transfected HEK293 cells were seeded on glass coverslips (Matsunami) coated with poly-L-lysine solution (Sigma-Aldrich) then incubated for 4–10 hours ...
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bioRxiv - Neuroscience 2022Quote: ... Neuro-2a (N2a) and HEK293 cells were maintained in Dulbecco’s Modified Eagle’s Medium (DMEM) high glucose (Sigma-Aldrich) supplemented with 10% (v/v ...
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bioRxiv - Developmental Biology 2019Quote: ... HEK293 cell viability after iOn plasmids transfection was assessed by dye exclusion with Trypan blue solution (0.4%, Sigma). FP expression was either assayed by flow cytometry ...
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bioRxiv - Neuroscience 2021Quote: Human embryonic kidney (HEK293) cells were maintained in a 37°C/5% CO2 atmosphere in DMEM:F12 (Sigma, D6421) supplemented with 10% foetal bovine serum (ClonTech ...
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bioRxiv - Cancer Biology 2021Quote: ... HEK293T and HEK293-FT cells were maintained in DMEM supplemented with 10% FBS,1 mM sodium pyruvate (Sigma), 2 mM L-glutamine (Sigma) ...
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bioRxiv - Cell Biology 2022Quote: HEK293-FLP cells stably expressing UBC:DOR-APEX2 were plated onto poly-L-lysine (Sigma Aldrich, Cat # P8920-100ML) coated coverslips in wells of a 24-well plates ...
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bioRxiv - Microbiology 2023Quote: HeLa cells and HEK293 cells were maintained in Dulbecco’s modified Eagle’s medium (DMEM) supplemented with 10% foetal bovine serum (Sigma) at 37°C in 5% (V/V ...
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bioRxiv - Neuroscience 2024Quote: ... SH3BP2-Flag was expressed in Hek293 cell and purified using anti-FLAG agarose beads (A2220-5ML, Sigma-Aldrich) and eluted with FLAG peptide (F4799-4MG ...
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bioRxiv - Developmental Biology 2021Quote: ... 1.8 μg/mL VEGF Receptor Tyrosine Kinase Inhibitor II (VEGFR-TKI II; Calbiochem/EMD Millipore #676481-5MG) dissolved in DMSO was used to disrupt blood vessels57 and 100 μM γ-secretase inhibitor IX (DAPT ...
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bioRxiv - Developmental Biology 2021Quote: ... VEGF Receptor Tyrosine Kinase Inhibitor II (VEGFR-TKI II; Calbiochem/EMD Millipore #676481-5MG, 1.8 μg/mL) or equivalent amount of DMSO vehicle was added to media.
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bioRxiv - Neuroscience 2021Quote: ... we now infused .22ul of either saline or 2mM muscimol (a GABA-A receptor agonist; Sigma Aldrich). Our infusion targets differed by experiment ...
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bioRxiv - Biophysics 2021Quote: ... The receptor was concentrated to 20–30 mg/ml with a 100 kDa cut-off concentrator (Millipore). Protein purity and mono-dispersity were examined by Nu-PAGE and analytical size-exclusion chromatography.
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bioRxiv - Neuroscience 2021Quote: Primary antibodies and dilutions were: Anti-Purinergic Receptor P2RY14 rabbit (extracellular) (1:200, Sigma Aldrich, Cat#: P0119); GPR105 Polyclonal antibody rabbit (1:200 ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated with an antibody against the M2 muscarinic acetylcholine receptor (1:500 in PBS, MAB367, Millipore) for at least 24 hours ...
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bioRxiv - Immunology 2022Quote: ... sera were heat-inactivated at 56 °C for 30 minutes and treated with receptor destroying enzyme (Sigma) in a 1:4 mixture (5x dilution of sera) ...
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bioRxiv - Neuroscience 2022Quote: ... AMPA-receptor-mediated excitatory postsynaptic currents (EPSCs) were recorded in presence of picrotoxin (100 μM, Sigma Aldrich) to block GABAARs ...
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bioRxiv - Neuroscience 2024Quote: ... Aladdin) or β receptor agonist isoprenaline (2 mg/mL, 4 mg/mL, n = 7, I5627, Sigma-Aldrich) or β receptor antagonist propranolol (2·5 mg/mL ...
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bioRxiv - Bioengineering 2023Quote: ... The stimulation of ACh to muscarinic receptors was blocked by addition of 10 nM Atropine (Sigma Aldrich). In the presence of Atropine ...
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bioRxiv - Neuroscience 2024Quote: ... Receptor cDNAs in the various pIRES2 constructs were transfected into Chinese Hamster Ovary-K1 (CHO-K1; Sigma) cells cultured in Dulbecco’s Modified Eagle Medium Nutrient Mixture F-12 (DMEM/F12 ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... anti-phosphorylated (Ser897)-N-methyl-D-aspartate receptor (anti phosphoNMDAR cat.#ABN99, Merck Millipore, Burlington, MA, USA), anti-total-calcium/calmodulin-dependent protein kinase II (anti-tCaMK ...
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bioRxiv - Plant Biology 2019Quote: ... Immuno-blotting was performed using anti-ubiquitin antibody and anti-His (Sigma, USA).
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bioRxiv - Biochemistry 2020Quote: 25 μl of the monoclonal α-His antibody (Catalog-ID H1029, Sigma-Aldrich) were mixed with 100 μl buffer comprising 50 mM HEPES-NaOH ...
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bioRxiv - Cell Biology 2021Quote: Ezrin constructs in pET-28a(+) (N-terminal hepta-His tag, Novagen, Madison, WI): hEzrin wildtype (His-Ezrin/WT ...
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bioRxiv - Biochemistry 2020Quote: ... The Gag-His6 protein was loaded onto a HIS-select affinity column (Sigma), washed (20 mM Tris-HCl ...
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bioRxiv - Biophysics 2021Quote: His-tagged human PCNA protein was purchased from Sigma Aldrich (catalogue number SRP5117), at a concentration of 6.6 μM ...
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bioRxiv - Cell Biology 2021Quote: ... SC –leu-trp plates and SC –leu-trp-his + 10mM 3-aminotriazole (Sigma) plates ...
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bioRxiv - Neuroscience 2020Quote: ... and HIS dihydrochloride (Sigma-Aldrich; 10 μg/10 μl; 9 mM; 90 nmol), as well as a combination of 2 substances (ALA 90 μg with BAM8-22 1 μg in 10 μl ...
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The formation of a fuzzy complex in the negative arm regulates the robustness of the circadian clockbioRxiv - Molecular Biology 2022Quote: ... and Western blotting with primary anti-His at 1:1000 (Sigma Aldrich, SAB1305538), secondary anti-Mouse at 1:10000 (ThermoFisher ...
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bioRxiv - Molecular Biology 2022Quote: ... which coeluted with His-TrxA or monoclonal anti-polyHistidine-Peroxidase antibody (Sigma-Aldrich) for the detection of recombinant His-tagged TrxA ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were washed with a washing solution of 4% HI-FBS (#F4135; Sigma) in PBS and permeabilized with 0.3% Triton X-100 (#T8787 ...
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bioRxiv - Synthetic Biology 2021Quote: ... and Guanidine Hydrochloride (Serva, 24205.02) via Hi-Trap Desalting column (Sigma Aldrich GE17140801) for further experiments ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was incubated with 15 ml HIS-Select Nickel Affinity Gel (Sigma) for 1 h at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Fractions containing His-Pap1 were pooled and concentrated using 30,000 MWCO concentrators (Millipore).
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bioRxiv - Biochemistry 2022Quote: ... cleared cell lysates were captured by Ni-NTA His-bind resin (Merk Millipore) equilibrated in high salt buffer (50 mM Tris pH8 ...
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bioRxiv - Molecular Biology 2022Quote: These His-tagged protein lysates were combined with 1 ml nickel beads (Sigma) and incubated at 4 °C/2 hours/rolling ...
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bioRxiv - Microbiology 2022Quote: ... purified from bacterial lysates on a nickel affinity resin (His-Select, Sigma-Aldrich) as described (12) ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS, Sigma-Aldrich, #12306C), 1% GlutaMAX (200 mM ...
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bioRxiv - Microbiology 2024Quote: ... followed by western blotting was using anti-His primary antibody (Sigma-Aldrich, H1029) and anti-mouse IgG HRP conjugate (Promega ...
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bioRxiv - Immunology 2024Quote: ... 10X hi ovomucoid inhibitor stock solution contained 300 mg BSA (Sigma-Aldrich, #A8806), 300 mg ovomucoid trypsin inhibitor (Worthington ...
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bioRxiv - Biochemistry 2024Quote: ... The medium was then loaded on a His-select Nickel affinity column (Millipore) equilibrated in 50 mM NaH2PO4 ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was then incubated with HIS-Select nickel affinity gel (Sigma-Aldrich) for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... Affinity chromatography (HIS-Select™ Nickel Affinity Gel; #P-6611; Sigma Aldrich Inc.) was then used to purify His6-tagged caltubin from the cell lysate and the protein was eluted with 125 mM imidazole (in lysis buffer) ...