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Citations for Millipore Sigma :
851 - 900 of 10000+ citations for Recombinant Mouse CD19 Antigen His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... Cells were washed with a washing solution of 4% HI-FBS (#F4135; Sigma) in PBS and permeabilized with 0.3% Triton X-100 (#T8787 ...
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bioRxiv - Synthetic Biology 2021Quote: ... and Guanidine Hydrochloride (Serva, 24205.02) via Hi-Trap Desalting column (Sigma Aldrich GE17140801) for further experiments ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was incubated with 15 ml HIS-Select Nickel Affinity Gel (Sigma) for 1 h at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Fractions containing His-Pap1 were pooled and concentrated using 30,000 MWCO concentrators (Millipore).
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bioRxiv - Biochemistry 2022Quote: ... cleared cell lysates were captured by Ni-NTA His-bind resin (Merk Millipore) equilibrated in high salt buffer (50 mM Tris pH8 ...
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bioRxiv - Immunology 2024Quote: ... 10X hi ovomucoid inhibitor stock solution contained 300 mg BSA (Sigma-Aldrich, #A8806), 300 mg ovomucoid trypsin inhibitor (Worthington ...
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bioRxiv - Microbiology 2022Quote: ... purified from bacterial lysates on a nickel affinity resin (His-Select, Sigma-Aldrich) as described (12) ...
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bioRxiv - Biochemistry 2024Quote: ... The medium was then loaded on a His-select Nickel affinity column (Millipore) equilibrated in 50 mM NaH2PO4 ...
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bioRxiv - Microbiology 2024Quote: ... followed by western blotting was using anti-His primary antibody (Sigma-Aldrich, H1029) and anti-mouse IgG HRP conjugate (Promega ...
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bioRxiv - Biochemistry 2022Quote: ... The supernatant was then incubated with HIS-Select nickel affinity gel (Sigma-Aldrich) for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... Affinity chromatography (HIS-Select™ Nickel Affinity Gel; #P-6611; Sigma Aldrich Inc.) was then used to purify His6-tagged caltubin from the cell lysate and the protein was eluted with 125 mM imidazole (in lysis buffer) ...
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bioRxiv - Neuroscience 2024Quote: ... supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS, Sigma-Aldrich, #12306C), 1% GlutaMAX (200 mM ...
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bioRxiv - Molecular Biology 2024Quote: ... the protein was re-applied to His-Select Cobalt affinity gel (Sigma-Aldrich). The flow through fractions were pooled and concentrated (Vivaspin Turbo 3,000 MWCO ...
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bioRxiv - Genetics 2024Quote: ... supplemented with 10% Heat Inactivated Fetal Bovine Serum (HI-FBS) (Sigma, REF-F4135), 1% L Glutamine 200 mM (Gibco ...
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bioRxiv - Molecular Biology 2021Quote: ... The HA-tagged PGBD1 was eluted by 500 μl 100 μg/ml HA-peptide (Sigma) in 100 mMNaCl ...
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bioRxiv - Cell Biology 2019Quote: Immunoprecipitations of Flag-tagged proteins were performed using α-Flag M2 affinity beads (Sigma-Aldrich). For each immunoprecipitation reaction ...
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bioRxiv - Cell Biology 2019Quote: ... Washed cells were treated with TRITC tagged Concanavalin A (20 µg ml-1) (Sigma, USA) for 1 h at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... FLAG-tagged proteins were eluted using FIB containing 0.1 mg/mL FLAG peptide (Sigma Aldrich). All immunoprecipitations were performed on three independent biological replicates.
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bioRxiv - Cancer Biology 2021Quote: ... All FLAG-tagged proteins were purified with anti-FLAG® M2 Affinity Gel (Sigma-Aldrich) in a native condition using the FLAG peptide elution ...
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bioRxiv - Molecular Biology 2020Quote: ... FLAG-tagged proteins were eluted by 60 μl of 3x FLAG® Peptide (Sigma F4799) (diluted in Tris-buffered saline (TBS ...
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bioRxiv - Developmental Biology 2019Quote: ... Immunoprecipitation of Flag-tagged UNC-3 was performed using Flag antibody coated beads (Sigma, #A2220). For the IP ...
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bioRxiv - Microbiology 2020Quote: ... Western blots targeting FLAG-tagged proteins were performed using FLAG® M2 monoclonal antibody (Sigma) as described previously (49).
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bioRxiv - Physiology 2021Quote: ... followed by incubation with fluorescent-tagged carboxylate-coated 2 um size latex beads (L3030, Sigma) for 3 hours ...
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bioRxiv - Developmental Biology 2021Quote: ... GST-tagged Pros-S-HD in soluble fraction was purified using Glutathione-Agarose beads (Sigma). The bound proteins were eluted in elution buffer (50 mM Tris ...
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bioRxiv - Microbiology 2021Quote: ... FLAG-tagged proteins were eluted using FIB containing 0.1 mg/mL FLAG peptide (Sigma Aldrich). After cell lysis ...
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bioRxiv - Genetics 2022Quote: V5H6-tagged FRQ was immunoprecipitated with 20 μL of V5 agarose (Sigma-Aldrich, Catalog #7345) from 2 mg of centrifugation-cleared lysate ...
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bioRxiv - Microbiology 2022Quote: ... and FLAG tagged S-protein was stained using anti-FLAG (clone M2, Sigma-Aldrich, F1804). SARS-CoV-2 Spike Antibody (ProSci ...
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bioRxiv - Neuroscience 2023Quote: ... GST-α synuclein fusion protein tagged at the N-terminus was obtained from Sigma Aldrich. 200 µg of GST-α synuclein was incubated with the equivalent amount of purified His-spectrin at 4°C overnight in the presence of glutathione sepharose beads ...
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bioRxiv - Microbiology 2023Quote: ... Western blots targeting FLAG-tagged proteins were performed using FLAG® M2 monoclonal antibody (Sigma) as described previously [24 ...
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bioRxiv - Genetics 2023Quote: ... FLAG-tagged proteins were immunopurified using preformed complexes of anti-FLAG M2 antibody (Sigma Aldrich) and protein G Sepharose (GE healthcare).
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bioRxiv - Biochemistry 2024Quote: ... FLAG tagged PFD3 and PFD5 were detected using anti-FLAG rabbit antibody (1:5,000, Sigma), FLAG tagged PFD5 in combination with A3B and cMyc was detected using anti-FLAG mouse antibody (1:1,000 ...
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bioRxiv - Plant Biology 2023Quote: ... The Flag-tagged proteins were then detected with Flag-HRP antibody (Sigma A8592, 1:5000); Endogenous PIF3 was detected with PIF3 antibody (Agrisera AS163954 ...
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bioRxiv - Immunology 2023Quote: ... Co-IP of FLAG-tagged proteins was performed by incubating M2 anti-FLAG resin (Sigma) with clarified cell lysates for 2 h at 4°C with end-over-end rotation ...
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bioRxiv - Cell Biology 2024Quote: ... Flag-tagged Scube2 was detected by using polyclonal α-FLAG antibodies (Sigma, St. Louis, USA). GAPDH ...
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bioRxiv - Biochemistry 2024Quote: ... Fractions containing pure polyhistidine-tagged proteins were pooled and concentrated by centrifugation filtration (EMD Millipore; using appropriate MWCO ...
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bioRxiv - Immunology 2022Quote: ... were coated with respective antigen for 2h at RT and then blocked with 5% BSA (Sigma-Aldrich). 50 μl 1:50 diluted plasma sample or monoclonal Abs was added to the wells and incubated overnight at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... MEFs were immortalized with SV40 large T-antigen by transfection using GeneJuice transfection reagent (Merck Millipore 70967).
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bioRxiv - Cell Biology 2020Quote: ... Antigen retrieval was performed by microwaving sections in preheated 0.01 M citrate buffer pH 6 (Sigma Aldrich) for 5 min.
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bioRxiv - Developmental Biology 2022Quote: ... antigen unmasking was performed either in 10 mM sodium citrate buffer (pH 6.0; S1804-500G, Sigma-Aldrich) for 12 min at 97°C ...
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bioRxiv - Neuroscience 2023Quote: ... underwent antigen retrieval for 30 minutes at 98°C in citrate buffer (10 mM citrate, Sigma-Aldrich) 0.05% Tween 20 (pH 6.0 ...
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bioRxiv - Developmental Biology 2023Quote: ... antigen retrieval was performed by boiling the sections in 10 mM sodium citrate and 0.05% Tween20 (Sigma) for 10 min ...
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bioRxiv - Microbiology 2023Quote: ... the antigen-serum-bead mixture was transferred into a 96-well filter HTS plate (Millipore, Bedford, MA). The plate was washed 8 times with Buffer A followed by twice with PBS on a vacuum manifold as described previously [26] ...
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bioRxiv - Microbiology 2023Quote: ... rodentium antigens were produced by homogenization of cultured bacterial pellet in lysis buffer (CellLytic B, Sigma-Aldrich) followed by centrifugation and harvesting of the soluble protein antigens ...
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bioRxiv - Immunology 2021Quote: ... Recombinant GST-p50 subunit D434-969 was purchased from Sigma-Aldrich. Binding reactions were performed for 20 minutes at room temperature with 20 pmol annealed primers ...
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bioRxiv - Immunology 2021Quote: Recombinant cytokines were purchased from indicated suppliers: IFN-β1a (Sigma, #IF014); IFN-γ (Sigma ...
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bioRxiv - Genomics 2020Quote: ... 1000 U/ml recombinant leukemia inhibitory factor (LIF/ESGRO) (Merck Millipore), and 50U/ml Penicillin/Streptomycin (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2019Quote: ... Following 12 h recombinant expression in Rosetta Competent Cells (Millipore, #70954) at 12° C with ampicillin and chloramphenicol selection ...
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bioRxiv - Bioengineering 2019Quote: ... 100 µg/ml human recombinant Delta-1 or human IgG (Sigma) was added ...
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bioRxiv - Biophysics 2021Quote: Recombinant HSA expressed in Pichia pastoris was purchased from Sigma-Aldrich (#A7736 ...
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bioRxiv - Neuroscience 2021Quote: ... and with or without 0.3 µM recombinant BirA (Sigma-Aldrich; SRP0417) (Howarth and Ting ...