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Citations for Millipore Sigma :
801 - 850 of 10000+ citations for Recombinant Human X Linked Inhibitor of Apoptosis AVI tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2022Quote: ... 150 mM NaCl, 0.1% SDS, 1% Triton X-100 and 0.5% sodium deoxycholate, supplemented with protease inhibitors [“Complete”; Sigma-Aldrich, St. Louis, MO]) and frozen prior to assay ...
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bioRxiv - Microbiology 2021Quote: ... and the cells were lysed in TX-100 lysis buffer (300mM NaCl; 50mM Tris-HCl, pH 7.5; 0.5% Triton X-100; β-mercaptoethanol, Sigma protease inhibitor cocktail) for 10 min on ice ...
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bioRxiv - Molecular Biology 2020Quote: ... 1% Triton X-100 and protease inhibitor) and 1/10 volume was kept as input before incubation with M2 dynabeads (Sigma-Aldrich, M8823) for 40 h at 4°C ...
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bioRxiv - Biochemistry 2021Quote: ... cells were washed with PBS and treated with lysis buffer (1% Triton X-100, 0.2% SDS and cOmplete EDTA-free protease inhibitor cocktail, COEDTAF-RO Sigma-Aldrich, in PBS). Upon scraping ...
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miRNA activity contributes to accurate RNA splicing in C. elegans intestine and body muscle tissues.bioRxiv - Molecular Biology 2019Quote: ... 1% Triton X-100 and 1 tablet of protease inhibitor for every 10ml of lysis buffer (Roche cOmplete ULTRA Tablets, Sigma, cat 5892791001). The lysed samples were subjected to sonication using the following settings ...
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bioRxiv - Plant Biology 2021Quote: ... 10 mM MgCl2, 250 mM sucrose, 0.5% Triton X-100, 5 mM 2-mercaptoethanol and 1X protease inhibitor cocktail [Millipore-Sigma, Cat# P9599]), and carefully overlaid on top of 500 μL of nuclear resuspension buffer 3 (20 mM Tris-HCl ...
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bioRxiv - Neuroscience 2022Quote: ... 150 mM NaCl, 5 mM EDTA, 1% Triton X-100, 1 mM sodium pervanadate, and protease inhibitor cocktail [1:100, Sigma, catalog #P8340]) at 4°C for 30 min ...
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bioRxiv - Cell Biology 2023Quote: Cells were washed with ice-cold phosphate-buffered saline (PBS) and lysed in extraction buffer (1% (v/v) triton X-100 and protease inhibitor (Sigma-Aldrich, 11836170001) in PBS) ...
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bioRxiv - Physiology 2023Quote: ... permeabilized with 0.1% Triton-X 100 (X-100, Sigma-Aldrich) in 4% PFA/HBSS for 10 min ...
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bioRxiv - Neuroscience 2024Quote: ... cells were permeabilized in 0.02% Triton X (Sigma X-100) in PBS (PBST ...
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bioRxiv - Neuroscience 2023Quote: ... and 0.3% Triton X-100 (Cat#X-100, Sigma-Aldrich) in TBS to yield a final concentration of 10 µg/ml anti-BDNF ...
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bioRxiv - Molecular Biology 2023Quote: ... and Triton-X-100 (1% v/v) (Sigma, X-100) directly into the culture medium ...
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bioRxiv - Biochemistry 2021Quote: Human IgM purified from human serum (Sigma) was digested with trypsin ...
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bioRxiv - Neuroscience 2020Quote: Soluble and insoluble human brain samples and mouse brain samples were homogenized in PBS with a protease inhibitor cocktail (catalog no. 11873580001; Sigma Aldrich) with 3.2 mm stainless steel beads ...
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bioRxiv - Neuroscience 2024Quote: Proteins from human cerebral cortex and whole mouse brain were extracted using RIPA buffer (MilliporeSigma) with Protease Inhibitor cocktail (Sigma P8340) as previously described [20] ...
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bioRxiv - Molecular Biology 2020Quote: ... and FLAG-tagged ribosomes were purified using M2 FLAG-affinity resin (Sigma), as described previously (Inada et al. ...
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bioRxiv - Microbiology 2019Quote: ... and tagged proteins were detected with mouse anti-GFP (Sigma, 1:5,000), rat anti-HA (Sigma 3F10 clone ...
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bioRxiv - Genetics 2020Quote: ... anti-mouse secondary antibody tagged with alkaline phosphatase (Sigma, catalogue no: A3562), CDP star (sigma) ...
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bioRxiv - Microbiology 2021Quote: ... FLAG-tagged proteins were eluted by competition using the FLAG peptide (Sigma).
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bioRxiv - Biochemistry 2022Quote: ... His6-tagged proteins were purified with Ni-NTA His-Bind Superflow (Novagen) and dialyzed against PBS using a Pur-A-Lyzer™ Midi Dialysis Kit (Merck).
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bioRxiv - Neuroscience 2019Quote: ... Flag-tagged proteins were immunoprecipitated using anti-Flag-M2 affinity gel (Sigma). Immunoprecipitates were incubated for at least 2 h at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... FLAG-tagged TSC2 was immunoprecipitated with ANTI-FLAG® agarose beads (Sigma) for 2 hours at 4°C ...
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bioRxiv - Microbiology 2019Quote: ... HA-tagged proteins were recognized using a murine antiHA antibody (Sigma-Aldrich) and then an antiMouse IgG-peroxydase antibody (KPL) ...
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bioRxiv - Molecular Biology 2020Quote: ... TAP tagged proteins was captured using IgG sepharose fast flow beads (Sigma) and proceeded as described (33) ...
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bioRxiv - Biochemistry 2019Quote: ... Strep-II-tagged proteins were bound to Strep-Tactin resin (EMD Millipore), washed with lysis buffer ...
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bioRxiv - Molecular Biology 2019Quote: ... anti-PAP 1:1000 (for detecting TAP-tagged proteins) (Sigma-Aldrich, P1291), anti-Rad53 1:2000 (abcam ab104232) ...
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bioRxiv - Molecular Biology 2023Quote: ... Flag-tagged ZC3H11A was immunopurified with anti-Flag(M2) magnetic beads (Sigma) overnight at 4°C ...
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bioRxiv - Neuroscience 2024Quote: ... After incubation with peroxidase-tagged secondary antibodies (1:2000 diluted, Sigma-Aldrich), membranes were revealed with an ECL-plus chemiluminescence western blot kit (Amershan-Pharmacia Biotech).
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The conserved membrane-proximal domain of Sbh1/ Sec61β guides signal peptides into the Sec61 channelbioRxiv - Biochemistry 2024Quote: ... followed by HRP-tagged anti-rabbit secondary antibody (1:10,000, Sigma-Aldrich).
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bioRxiv - Microbiology 2023Quote: ... FLAG-tagged protein signals were detected with anti-FLAG (Sigma-Aldrich, F1804) in combination with anti-mouse secondary antibody (Invitrogen ...
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bioRxiv - Cell Biology 2023Quote: ... or Glutathione High Capacity Magnetic Agarode Beads (for GST-tagged proteins, SIGMA). After 2 extensive washing with binding buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... HRP-tagged anti-rabbit and anti-mouse secondary antibodies (SA) (Sigma-Aldrich). Densitometry values of the immunoblots were computed using GelQuant.Net software.
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Membrane-dependent actin polymerization mediated by the Legionella pneumophila effector protein MavHbioRxiv - Molecular Biology 2023Quote: ... HA tagged CPα was probed using a mouse anti-HA antibody (Sigma). Probed proteins were detected using donkey anti-rabbit IgG antibody ...
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bioRxiv - Microbiology 2024Quote: ... FLAG-tagged proteins were immunoprecipitated with FLAG-M2 antibody (F1804 Sigma-Aldrich) conjugated to protein G dynabeads (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2024Quote: ... FLAG-tagged Imp was pulled down with Magnetic M2 FLAG Beads (Sigma). Immunoprecipitations and washes were performed as described in the iCLIP2 protocol ...
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bioRxiv - Molecular Biology 2020Quote: Annexin V-FITC apoptosis assay was carried out as described in manufacturer’s protocol (APOAF, Sigma. St. Louis, MO). After transfected with Npac RNAi or control RNAi plasmid in 6-well dishes and selected for 4 days ...
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bioRxiv - Neuroscience 2020Quote: ... The TUNEL protocol was performed using a commercial kit (ApopTag® fluorescein in situ apoptosis detection kit, Millipore). Apoptotic cells were detected as localized bright green cells in a blue background (DAPI ...
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bioRxiv - Pathology 2021Quote: ... TUNEL assay was carried out on cryosections (15μm thickness) using Apoptag Peroxidase In Situ Apoptosis Detection Kit (Millipore) as instructed by the manufacturer ...
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bioRxiv - Cell Biology 2020Quote: TUNEL staining was carried out by using an ApopTag® Fluorescein in Situ Apoptosis Detection Kit (S7110, Sigma) according to the manufacturer’s instruction ...
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bioRxiv - Molecular Biology 2021Quote: Detection of apoptotic cells was determined by using ApopTag® Fluorescein In Situ Apoptosis Detection Kit (Sigma Aldrich). Paraffin sections were deparaffinized and hydrated as described and then treated with proteinase K (20 μg/mL ...
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bioRxiv - Cancer Biology 2021Quote: ... 500,000U Recombinant Mouse LIF Protein (Millipore Cat#ESG1107)) with feeders ...
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bioRxiv - Developmental Biology 2019Quote: ... and 1000 U/ml recombinant LIF (ESGRO, Millipore). 2i Medium (N2B27 ...
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bioRxiv - Cell Biology 2021Quote: ... Recombinant mTOR was purchased from Millipore (14-770) and recombinant 4EBP1 was purchased from Millipore (516678) ...
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bioRxiv - Microbiology 2021Quote: ... 50 ng/ml mouse recombinant EGF (Sigma-Aldrich), 1X B27 supplement (Gibco) ...
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bioRxiv - Microbiology 2019Quote: ... and incubated with recombinant protein (GenScript, Sigma, Tocris) of each analyte for 4 h at room temperature ...
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bioRxiv - Cell Biology 2019Quote: ... recombinant proteins of Ubiquitin (10µg) (Sigma-Aldrich, U6253) or human His6-ubiquitin (Boston Biochem ...
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bioRxiv - Immunology 2020Quote: ... Recombinant SLAMF7 protein (r-SLAMF7) was from Sigma. Purified anti-SLAMF7 (162.1 ...
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bioRxiv - Immunology 2020Quote: ... 5 μg/ml recombinant GAL4-TA (Sigma-Aldrich) was added for an additional 14 hours ...
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bioRxiv - Cell Biology 2022Quote: ... recombinant mouse EGF (0.025μg/ml, Sigma-Aldrich, #SRP3196) and bovine pituitary extract (30μg/ml ...
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bioRxiv - Pathology 2022Quote: ... recombinant anti-Cre (Merck Millipore, 69050, 1:1000) and revealed using Histofine reagent (Nichirei Biosciences) ...