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Citations for Millipore Sigma :
751 - 800 of 10000+ citations for IL 3 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... 3-bromobenzotrifluoride (Sigma B59004) was mixed with dSURF oil for refractive index modifications as per requirement ...
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bioRxiv - Biophysics 2023Quote: ... 3% Empigen (Millipore Sigma) was then added and the tube was rotated at 4 °C for 2-3 hr prior to centrifugation at 10,000 g for 30 min ...
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bioRxiv - Cell Biology 2023Quote: ... plus 3% BSA (Sigma) and were incubated overnight at 4°C on a shaker ...
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bioRxiv - Neuroscience 2023Quote: ... 3 KCl (Sigma #P5405), 1 MgSO4.7H2O (Sigma #M2773) ...
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bioRxiv - Neuroscience 2023Quote: ... 3 KCl (Sigma #P5405), 10 MgSO4.7H2O (Sigma #M2773) ...
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bioRxiv - Developmental Biology 2023Quote: ... 1X ITS+3 (Sigma) and 1.25mg/mL Bovine Serum Albumin ...
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bioRxiv - Molecular Biology 2023Quote: ... 3 μM CHIR99021 (Sigma), 1 μM PD0325901 (Sigma) ...
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bioRxiv - Bioengineering 2023Quote: ... 3 (Sigma-Aldrich GHS332), differentiating with tap water and acidic ethanol ...
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bioRxiv - Immunology 2023Quote: ... 3 mM EGTA (Sigma), 100 nM 2-APB (Sigma) ...
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bioRxiv - Neuroscience 2023Quote: ... 3 mM MgCl2 (Sigma), 0.1% Nonidet P40 Substitute (Sigma) ...
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bioRxiv - Pathology 2024Quote: ... SITE+3 (Sigma S5295), 25 mM blebbistatin (Toronto Research Chemicals B592500)) ...
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bioRxiv - Microbiology 2024Quote: ... DiBAC4(3) (Sigma-Aldrich) was added at a final concentration of 1 µg/ml ...
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bioRxiv - Genomics 2024Quote: ... and 3% BSA (Sigma). The primary and secondary antibodies specifications and dilutions are at 1:500 and 1:2000 ...
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bioRxiv - Biophysics 2024Quote: ... 1% phosphatase inhibitor cocktails #2 (Sigma P5726-1ML)/#3 (Sigma P0044-1ML), and one cOmplete protease inhibitor tablet (Sigma 04693159001) ...
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bioRxiv - Cancer Biology 2024Quote: ... and #3 (Sigma #P0044) used 1:100 each ...
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bioRxiv - Neuroscience 2024Quote: ... 3-octanol (Sigma, W358118), or a pleasant odor of 2.3-butanedione (Sigma ...
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bioRxiv - Cancer Biology 2024Quote: ... Nutlin-3 (Sigma N6287) was added to a final concentration of 5 μM (A549 and NCI-H460 ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant pH was adjusted to 8.0 and incubated with Ni2+-NTA His-binding resin (Novagen) that was pre-equilibrated with binding buffer (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... the blot was incubated with a mouse anti-His-tag primary antibody (Sigma-Aldrich, Steinheim, Germany), diluted 1:1 000 in TBS (10 mM TRIS/HCl (pH 7.5) ...
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bioRxiv - Biochemistry 2020Quote: ... The Trx-His(6) tag was cleaved by addition of 300 units of thrombin (Sigma T4648) directly to the eluate and the solution dialyzed (Slide-A-Lyzer dialysis cassettes ...
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bioRxiv - Plant Biology 2020Quote: ... The His6-SEC5B fusion protein was extracted and purified with Ni-NTA His bind resin (Novagen). The freshly extracted resin-bound GST or GST-XIK proteins were mixed with purified His6-SEC5B protein in protein interaction buffer and incubated at 4°C for 2 h with shaking ...
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bioRxiv - Biophysics 2020Quote: ... MBP and the His-tag were cleaved from the P domain using HRV 3C protease (Novagen). Cleaved P domain protein eluted from Ni-NTA resin and was further purified by size-exclusion chromatography using a Superdex 16/600 200 pg column (GE Healthcare ...
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bioRxiv - Neuroscience 2019Quote: ... or combined together (GPA2-FLAG/GPB5-His) and then treated with DSS (Sigma Aldrich, Oakville, ON). In some experiments ...
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bioRxiv - Immunology 2021Quote: ... using cross-reactive anti-SARS-CoV-1 Spike CR3022 or mouse anti-His tag (Sigma-Aldrich) as positive controls ...
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bioRxiv - Neuroscience 2019Quote: FXR1(1-379) was expressed as His- tagged fusion protein in Rosetta (DE3) pLysS cells (Novagen) in LB medium ...
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bioRxiv - Plant Biology 2021Quote: ... and GST were fused with a C-terminal octa-His tag in the pET42 vector (Novagen).
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bioRxiv - Biochemistry 2021Quote: ... The latter were then probed with mouse anti-His HRP conjugated antibody (1:400, Sigma-Aldrich) and visualized by chromogenic DAB reagent (Sigma).
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bioRxiv - Molecular Biology 2022Quote: ... Cleared supernatant was incubated with 20 μl His Mag Sepharose Ni beads (Sigma, GE28-9673-88) overnight ...
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bioRxiv - Physiology 2022Quote: ... The F4-2_Fab protein was first purified using cOmplete® His-tag purification resin (Sigma-Aldrich), and L6-F4-2 protein was first purified with CaptivA Protein A affinity resin (Repligen) ...
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bioRxiv - Neuroscience 2024Quote: ... and the supernatant subjected to Ni-NTA affinity purification (HIS-Select Nickel Affinity Gel (Sigma Aldrich)) ...
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bioRxiv - Biochemistry 2024Quote: ... and the protein solution was mixed with Co resin (His-select Cobalt Affinity Gel, Sigma Aldrich) for 1h at 4 °C ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified by centrifugation and applied to cOmplete His-tag purification resin (Sigma Aldrich) for 1 hour at 4 °C with rotation ...
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bioRxiv - Biochemistry 2024Quote: ... The resin-bound His-tagged proteins were washed with wash buffer containing 20mM imidazole (Sigma #12399) to eliminate non- specific binding and eluted 5 times subsequently with elution buffer (20mM Tris HCl pH7.5 ...
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bioRxiv - Biochemistry 2024Quote: ... The UbVs and Ube2d2S22R-Avi were purified by nickel-affinity chromatography with HIS-select resin (Sigma) followed by an overnight 3C digestion using in-house produced 3C protease ...
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bioRxiv - Molecular Biology 2024Quote: ... Clarified lysate was applied to 2.5 ml bed volume His-select Cobalt Affinity Gel (Sigma-Aldrich). The slurry was rotated in the cold room for an hour prior to application to a gravity flow column ...
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bioRxiv - Neuroscience 2021Quote: ... antisense: 5’-ACCGAUUGAAGUUAAUGUC-3’), Myef2 (sense: 5’-CUUUGGUGGAGUUGGCCGA-3’, antisense: 5’-UCGGCCAACUCCACCAAAG-3’) or siRNA universal negative control (SIC001, Sigma-Aldrich) were purchased from Sigma-Aldrich ...
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bioRxiv - Microbiology 2023Quote: ... Then the supernatant was separated by size to a large (>3 kDa) and small (<3 kDa) fraction using Amicon ultrafiltration system with 3 kDa filter (Millipore, Ireland). The fraction then filtered again through a 0.22 µm (Millipore).
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bioRxiv - Biochemistry 2023Quote: ... N-(3-Oxoocatnoyl)-L-HSL (>97%) (3-Oxo-C8) and N-(3-Oxododecanoyl)-L-HSL (>98%) (3-oxo-C12) were used as received from Sigma-Aldrich. N-(3-Hydroxy-7-cis-tetradecanoyl)-L-HSL (>95% ...
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bioRxiv - Immunology 2020Quote: ... for 2 days and then just IL-2 (20ng/ml) (Sigma-Aldrich) for a further 5 days ...
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bioRxiv - Immunology 2020Quote: ... 1 × AA and 50 U/ml IL-2 (Sigma-Aldrich; MO, USA), were treated in triplicate respectively with D+T NP or D+T solution ...
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bioRxiv - Molecular Biology 2021Quote: ... Lyophilized cytokines (TNF-α, IL-1α, C1q) were obtained from Sigma-Aldrich, resuspended in molecular biology grade water to make stock solutions ...
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bioRxiv - Neuroscience 2022Quote: ... Stock solutions of the cytokines (TNF-α, IL-1α, C1q-Sigma-Aldrich) were prepared in molecular biology grade water ...
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bioRxiv - Neuroscience 2022Quote: ... Stock solutions of the cytokines (TNF-α, IL-1α, C1q-Sigma-Aldrich) were prepared in molecular biology grade water ...
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bioRxiv - Cell Biology 2024Quote: ... interleukin 1b (recombinant murine Il-1b, Sigma-Aldrich, #I5271, 10 ng/mL) and interferone γ (recombinant mouse INFγ ...
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bioRxiv - Cell Biology 2020Quote: ... Lipin-1 siRNA 5’-GAAUGGAAUGCCAGCUGAA-3’ and 3’-UUCAGCUGGCAUUCCAUUC-5’ (Invitrogen; HSS118307 (Sigma); optineurin siRNA (Invitrogen 4392420) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Each mouse was intraperitoneal injected with 3 mL of 3% thioglycolate (Sigma-Aldrich) on day 1 and sacrificed using isoflurane on Day 3 ...
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bioRxiv - Neuroscience 2019Quote: ... Three additional small molecules were introduced on day 3: CHIR99021 (Sigma, 3 µM), SU5402 (R&D Systems ...
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bioRxiv - Neuroscience 2020Quote: ... after approximately 3 days in culture (DIV 3) cytosine β-D-arabinofuranoside (Sigma) was added to the hippocampal cultures in a 50% growth media exchange at a final concentration of 2 µM ...
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bioRxiv - Biochemistry 2021Quote: ... 1.7 g (8.9 mmol) 1-ethyl-3-(3- dimethylaminopropyl)carbodiimide (EDAC, Sigma Aldrich), and 1.2 g (8.9 mmol ...
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bioRxiv - Microbiology 2019Quote: ... and primers p181 (5’-GCGAAGATAACAGTGACTCTA-3’ and p54 (5’-CGGCTCTGATTAAATTCTGAAG-3’) (Sigma, UK).