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Citations for Millipore Sigma :
7651 - 7700 of 10000+ citations since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... The musculature of the FhNEJ was counterstained with phalloidin-tetramethyl rhodamine isothiocyanate (TRITC, Sigma-Aldrich) at a concentration of 200 µg/mL ...
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bioRxiv - Molecular Biology 2024Quote: ... and 5% glycerol (Sigma), supplemented with PhosStop (Roche ...
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bioRxiv - Molecular Biology 2024Quote: ... Peptides were resuspended in MS buffer containing 2% ACN in 1% Formic Acid (Sigma).
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bioRxiv - Molecular Biology 2024Quote: ... and 20 mM Sodium Azide (Sigma). The cells were washed with PBS and frozen at –80 °C as a dry pellet ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were resuspended in 2% TFA in isopropanol (IPA) (Sigma) and loaded on stage tips packed in-house with 2x layers of styrene-divinylbenzene (SDB-RPS ...
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bioRxiv - Molecular Biology 2024Quote: ... and 40 mM IAM (Sigma) and lysate was heated to 95 °C for 2x 5 mins followed by homogenization with a tip probe sonicator for 2x 20 sec cycles ...
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bioRxiv - Molecular Biology 2024Quote: ... 10 × 4 U leukemia inhibitory factor (LIF; Millipore), 1 μM MEK inhibitor PD0325901 (Mirdametinib ...
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bioRxiv - Molecular Biology 2024Quote: ... Flag (F1804, Sigma), 1:2000 ...
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bioRxiv - Molecular Biology 2024Quote: ... HeLa cultures were transduced with concentrated lentivirus at an MOI of 10 for 48 hours in 4 µg mL−1 polybrene (Sigma), then selected in normal growth media supplemented with appropriate antibody selection ...
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bioRxiv - Molecular Biology 2024Quote: ... and transferred to PVDF membranes (Millipore). Membranes were blocked with Blocking Buffer (Nacalai ...
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bioRxiv - Molecular Biology 2024Quote: Cell pellets were resuspended cold GdmCl lysis buffer containing 6 M Guanidinium Chloride (Sigma) and 100 mM Tris-HCl pH 8.5 (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... The zebrafish larvae were then terminally anaesthetised using MS-222 (Sigma) and homogenised in the Precellys 24 Tissue Homogeniser in 1X RIPA solution (Merck-Millipore ...
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bioRxiv - Immunology 2024Quote: ... 1 mg of as-synthesized SWCNT powder was added to 2 mg of (GT)xx oligonucleotide (Integrated DNA Technologies) 1 mL of 0.1 M NaCl (Sigma-Aldrich). Each sample was ultrasonicated using a 1/8′′ tapered microtip for 30 min at 40% amplitude in an ice bath (Sonics Vibracell VCX-130 ...
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bioRxiv - Molecular Biology 2024Quote: ... and cholesterol (catalog #C8667) were from Sigma-Aldrich. Dimethyl sulfoxide (catalog #BP231-1 ...
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bioRxiv - Molecular Biology 2024Quote: ... protease inhibitor cocktail (Sigma)) for 10 min on ice ...
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bioRxiv - Molecular Biology 2024Quote: ... HEK293T and HeLa cells were cultured in Dulbecco’s Modified Eagle Medium (Sigma), supplemented with 10% foetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2024Quote: ... and homogenised in the Precellys 24 Tissue Homogeniser in 1X RIPA solution (Merck-Millipore) before being centrifuged at 17 000 g for 15 min ...
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bioRxiv - Molecular Biology 2024Quote: ... procured from Sigma-Aldrich, were dissolved in ultrapure water to achieve the desired concentration ...
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bioRxiv - Immunology 2024Quote: ... the substrate D-luciferin (1.5 mg/100 μl in PBS; Sigma-Aldrich, Dorset, UK) was injected s.c ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.6% (v/v) IGEPAL CA-630 (Sigma-Aldrich, 18896-50ML), 5 mM DTT (TFS ...
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bioRxiv - Molecular Biology 2024Quote: ... The barcoded hydrogel beads were suspended in 1x First Strand buffer (TFS, 18080044) supplemented with 0.3% (v/v) IGEPAL CA-630 (Sigma-Aldrich, 18896-50ML). The 2x RT-lysis mixture for inDrops-1 protocol comprised ...
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bioRxiv - Molecular Biology 2024Quote: ... genomic DNA was extracted from cultures that finished sexual development using GeneElute – Mammalian Genomic DNA Miniprep Kit (Merck-Sigma, Germany). PCRs were done on specific genomic regions flanking an IES (Table S6 ...
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bioRxiv - Molecular Biology 2024Quote: ... The samples were then purified using a Microcon 3,000 MWCO centrifugal filter column (Milipore Sigma) spun at 12,000 rpm (4 °C) ...
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bioRxiv - Molecular Biology 2024Quote: All starting materials and solvents were purchased commercially from Sigma-Aldrich, Acros ...
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bioRxiv - Molecular Biology 2024Quote: Tight junction permeability was assessed by passage of 3000-5000 average molecular weight Fluorescein sothiocyanate-dextran (FD4, Sigma-Aldrich, St. Louis, MO) from the apical to basolateral side of well-differentiated human airway epithelia ...
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bioRxiv - Molecular Biology 2024Quote: Endosomal acidification inhibitors choloroquine (CQ) (200 µM) (C6628, Millipore Sigma, St. Louis, MO) and bafilomycin A1 (Baf A1 ...
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bioRxiv - Molecular Biology 2024Quote: ... with BSA (Sigma-Aldrich, USA) as protein standard (Zor & Selinger ...
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bioRxiv - Molecular Biology 2024Quote: ... at 1 in 1000 concentration and where appropriate PhosSTOP (Sigma) at a final concentration of 1 in 20 ...
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bioRxiv - Molecular Biology 2024Quote: ... washed with TBS-T buffer and incubated with mouse-produced monoclonal anti-polyHistidine-Peroxidase antibodies (Sigma-Aldrich) in a 1:1000 dilution in TBS-T buffer overnight at 4 °C ...
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Insight into the regulatory mechanism of the MFS transporter, SCO4121 by the MarR regulator, SCO4122bioRxiv - Molecular Biology 2024Quote: ... the WT SCO4122 and mutant M93L-SCO4122 proteins were transferred on to polyvinylidene difluoride (PVDF) membrane (Millipore) using transfer buffer (6g Tris base ...
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bioRxiv - Molecular Biology 2024Quote: ... and protease inhibitor cocktail (COmplete, Sigma-Aldrich, Taufkirchen, Germany). The cell lysates were centrifuged at 20,000 g for 1 h at 4°C and the supernatants were filtered with a 0.22 μm filter ...
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bioRxiv - Immunology 2024Quote: ... BHA (Cat# B1253) and Deferoxamine mesylate (Cat#D9533]) were sourced from Sigma Aldrich. PLX3397 (Cat# S7818) ...
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bioRxiv - Molecular Biology 2024Quote: Synechocystis cultures (50 mL) grown under different conditions were supplemented with 10 mM N-ethyl-maleimide (NEM, Sigma-Aldrich) and further cultivated for 1 h to block free cysteine residues ...
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bioRxiv - Molecular Biology 2024Quote: ... 1% protease inhibitor cocktail (Sigma-Aldrich), 0.1% Triton-X100) ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were then incubated with 4’,6-diamidino-2-phenylindole (DAPI, Sigma) in PBS for nuclear staining ...
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Insight into the regulatory mechanism of the MFS transporter, SCO4121 by the MarR regulator, SCO4122bioRxiv - Molecular Biology 2024Quote: ... Ciprofloxacin and apramycin was purchased from Sigma-Aldrich whereas all other drugs were purchased from Himedia.
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bioRxiv - Molecular Biology 2024Quote: ... The commercial antibodies used in the analysis were Anti-Actin Antibody (A0480; Sigma-Aldrich), anti-HY5 (AS12 1867 ...
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bioRxiv - Molecular Biology 2024Quote: ... then after preclearing with protein A–Sepharose beads (Sigma-Aldrich), HY5 antibody (AS12 1867 ...
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bioRxiv - Molecular Biology 2024Quote: ... and 1% phosphatase inhibitor cocktail (Sigma) was used to lyse the cells for at least 30 minutes at 4°C ...
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bioRxiv - Molecular Biology 2024Quote: Cold PBS was used to wash the cells and RIPA buffer supplemented with 2% (v/v) protease inhibitor cocktail (Sigma) and 1% phosphatase inhibitor cocktail (Sigma ...
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Insight into the regulatory mechanism of the MFS transporter, SCO4121 by the MarR regulator, SCO4122bioRxiv - Molecular Biology 2024Quote: ... The eluted proteins were further concentrated using Amicon® Ultra-4 centrifugal filter (Millipore) and contaminants were removed using Gel-filtration chromatography ...
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bioRxiv - Immunology 2024Quote: ... or IgA (1/750) (Sigma Aldrich). As substrate 1.0 mg/ ml o-phenylendiamine (OPD ...
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eATP/P2X7R axis drives nanoparticle induced neutrophil recruitment in the pulmonary microcirculationbioRxiv - Immunology 2024Quote: ... Cytospin slides were stained with May-Grünwald-Giemsa staining (Sigma Aldrich, Taufkirchen, Germany) to identify macrophages ...
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bioRxiv - Immunology 2024Quote: ... and 0.5 mg/ml DNase I (Sigma) at 37°C for 25 minutes ...
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bioRxiv - Immunology 2024Quote: ... Peripheral blood mononuclear cells (PBMC) from each donor were obtained by standard Ficoll-Paque (Sigma-Aldrich) gradient centrifugation and cells were liquid nitrogen-frozen in fetal bovine serum (FBS ...
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bioRxiv - Immunology 2024Quote: ... Sigma-Aldrich)/laminine (1:500, Sigma-Aldrich)-coated tissue culture treated 24 well plates and treated with IFN-γ (1ng/ml ...
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bioRxiv - Immunology 2024Quote: ... + 0.1% Triton X-100 (Sigma-Aldrich)) ...
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bioRxiv - Immunology 2024Quote: ... gradient centrifugation and cells were liquid nitrogen-frozen in fetal bovine serum (FBS, Biowest) and DMSO (1:10, Sigma-Aldrich). PBMC from AIE patients were taken at the moment of initial disease onset and for Ri01 ...
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bioRxiv - Immunology 2024Quote: ... they were plated and differentiated in polyornithine (1:5, Sigma-Aldrich)/laminine (1:500 ...
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bioRxiv - Immunology 2024Quote: ... 5 mg of mouse VAT tissue was homogenized in 300 μl of RIPA lysis buffer (R0278-50ML, Sigma-Aldrich) containing protease inhibitor and centrifuged at 12,000g for 10min at 4 °C ...