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Citations for Millipore Sigma :
651 - 700 of 10000+ citations for Recombinant Human X Linked Inhibitor of Apoptosis AVI tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... and Recombinant mouse LIF (Sigma, #ESG1107). Additionally ...
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bioRxiv - Immunology 2021Quote: ... Recombinant purified Angiotensin II (Sigma – A9525) at 1 μM with 2-fold serial dilutions ...
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bioRxiv - Immunology 2022Quote: ... recombinant complement component C3 (Millipore Sigma), recombinant CD4 (Sigma Aldrich ...
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bioRxiv - Bioengineering 2022Quote: ... recombinant leukemia inhibitory factor (SRP3316; Sigma), growth hormone (869008 ...
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bioRxiv - Neuroscience 2023Quote: ... and/or recombinant Fibronectin (Sigma-Aldrich) were applied as described using a range of concentrations (μg/ml).
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... 100 mIU/ml recombinant hCG (Sigma), and 10 mg/ml of human serum albumin (Sage) ...
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bioRxiv - Molecular Biology 2024Quote: ... 1000U/ml ESGro recombinant LIF (Millipore), 1uM PD0325901 (R&D Systems) ...
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bioRxiv - Biophysics 2023Quote: ... superoxide dismutase (bovine SOD, recombinant, Sigma) was added from a ≥50,000 U/mL stock prepared in assay buffer ...
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bioRxiv - Cancer Biology 2023Quote: BG4 recombinant antibody (Sigma Aldrich MABE917)- ChIP
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bioRxiv - Cell Biology 2024Quote: ... The recombinant laminin (L6274, Sigma‒Aldrich) we used was almost identical to Biolaminin 511 LN because nearly all the laminins in L6274 were laminin-511 (Wondimu et al. ...
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bioRxiv - Bioengineering 2024Quote: ... 100 mIU/mL recombinant hCG (Sigma), 500 ng/mL of androstenedione (Sigma) ...
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bioRxiv - Cell Biology 2021Quote: A549 cells expressing GFP-tagged EGFR were obtained from Sigma. Cells were plated on Edge plates (Thermo Scientific ...
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bioRxiv - Genetics 2019Quote: ... The TAP-tagged proteins were probed with PAP (Sigma-Aldrich) (1:2,000 ...
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bioRxiv - Cell Biology 2020Quote: ... FLAG-tagged proteins were immunoprecipitated with αFLAG (clone M2, Sigma) beads (Wang et al ...
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bioRxiv - Microbiology 2023Quote: N-terminal His-tagged KtrD cloned into pRSFDuet-1 (Novagen) was overexpressed in Escherichia coli BL21 (DE3 ...
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bioRxiv - Biochemistry 2023Quote: ... 25 uL of (His)6-tagged TEV-protease (Sigma-Aldrich) was added and the solution incubated overnight at 4°C.
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bioRxiv - Plant Biology 2024Quote: ... GST-tagged protein fractions were incubated with thrombin (Sigma, Germany) at 4°C overnight for cleavage of the GST-tag (Step 2 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... μL Triton lysis buffer (50 mM Tris pH 7.4, 150 mM NaCl, 1% Triton X-100) supplemented with mammalian protease inhibitor (Sigma-Aldrich). 150 μL 2× Laemmli SDS-PAGE loading buffer supplemented with 10% 2-mercaptoethanol (Sigma-Aldrich ...
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bioRxiv - Cancer Biology 2020Quote: ... PANC02 cells were adapted to toxic concentrations of AKT inhibitor X (10-DEBC hydrochloride, SIGMA, CAS number 925681-41-0), by continuous growth with AKTi through a step-by-step increase in concentration until reaching toxic concentrations (50 µM ...
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bioRxiv - Molecular Biology 2019Quote: ... cells were rapidly thawed and resuspended in PBS supplemented with 0.5% (v/v) Triton X-100 and cOmplete EDTA-free Protease Inhibitor Cocktail (Sigma-Aldrich) (PI ...
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bioRxiv - Biochemistry 2019Quote: ... 1 mM EGTA and 1% Triton X-100 with protease and phosphatase inhibitor cocktails both from (Millipore, Billierica, MA, USA). The soluble fractions of cell lysates were isolated by centrifugation at 15000g for 15 minutes at 4°C ...
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bioRxiv - Immunology 2019Quote: ... 1×106 neutrophils were lysed in Tris lysis buffer (0.2M Tris, 1% Triton X-100, 1% sodium orthovandate) containing protease inhibitor cocktail (Sigma Aldrich) for 30 minutes on ice with occasional mixing ...
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bioRxiv - Microbiology 2021Quote: ... 150 mM NaCl, 0.5 mM EDTA, 1% Triton-X-100, 10 mg/ml lysozyme, 1x EDTA-free protease inhibitors [Sigma Aldrich]). Samples were incubated for 25 min at 37°C ...
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bioRxiv - Microbiology 2022Quote: ... Supernatant was discarded and cells resuspended in ice cold QUEEN buffer (50 mM HEPES, 200 mM KCl, 1 mM MgCl2, 0.05% Triton X-100, protease inhibitor cocktail (Sigma, GB), pH adjusted to 7.7 ...
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bioRxiv - Molecular Biology 2022Quote: ... 1.5 mM MgCl2, 140 mM KCl, 1% Triton-X-100, 0.5 mM DTT, 1 mM PMSF, 1X protease inhibitor cocktail [Sigma-Aldrich, #P8340] ...
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bioRxiv - Molecular Biology 2022Quote: ... 150 mM NaCl, 1% Triton X-100, 1 mM dithiothreitol [DTT], 0.2% sarkosyl acid and protease inhibitor cocktail [Sigma, P8340]). Protein samples were subjected to SDS-PAGE and transferred to Immobilon-P membranes (Millipore) ...
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Role of autophagy in sepsis-induced skeletal muscle dysfunction, whole-body metabolism, and survivalbioRxiv - Cell Biology 2021Quote: ... Approximately 15-30 mg of muscle tissue was homogenized in PBS based lysis buffer (0.1% Triton X-100, 10μl/ml of a protease inhibitor cocktail (Sigma-Aldrich # P8340)) ...
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bioRxiv - Neuroscience 2021Quote: ... 150 mM NaCl, 1% sodium dodecyl sulfate [SDS], 0.5% sodium deoxycholate, 1% Triton X-100, 1x protease inhibitor cocktail [Sigma-Aldrich; catalog # P8849] ...
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bioRxiv - Cell Biology 2019Quote: ... Cell pellets were lysed in 1ml lysis buffer (20mM Tris-HCl pH 7.4, 300mM NaCl, 1% [vol/vol] Triton X-100 and protease inhibitor cocktail (Sigma-Aldrich)) ...
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bioRxiv - Microbiology 2019Quote: The caudal quarter of the flash frozen brain tissue was homogenized in radioimmunoprecipitation assay buffer (1% Triton-X, 0.1% SDS, 1X PBS) and 1% phosphatase inhibitor cocktail (P5726-1ML, Sigma-Aldrich) and 1% protease inhibitor cocktail (P8340-1ML ...
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bioRxiv - Plant Biology 2021Quote: ... 10%glycerol, 1 mM DTT, 1 mM EDTA, 0.5% Triton X-100 and 1% [v/v] protease inhibitor cocktail; Sigma-Aldrich). Insoluble debris was pelleted by centrifugation for 20 min with 4000 rpm at 4°C ...
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bioRxiv - Plant Biology 2021Quote: ... 5 mM EDTA, 1 mM NaF, 10 mM DTT, 0.1% Triton X-100 and 1% [v/v] protease inhibitor cocktail; Sigma-Aldrich). Protein extracts were boiled at 95°C for 10 min in 1x sodium-dodecyl sulfate (SDS ...
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bioRxiv - Immunology 2021Quote: Lung homogenate supernatants were collected by centrifugation at 2000 x g at 4°C and stored at −80°C with protease inhibitor cocktail (Sigma). Quantification of TNF-α ...
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bioRxiv - Plant Biology 2021Quote: ... 1.7 M sucrose, 0.5% Triton X-100, 5 mM 2-mercaptoethanol and 1x protease inhibitor cocktail [Millipore-Sigma, Cat# P9599]). The sample was centrifuged at 16,000 g at 4°C for 45 min ...
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bioRxiv - Cell Biology 2021Quote: ... HA-tagged or direct knockout parasites lines from single T25 flasks were harvested and lysed in 50 μl lysis buffer (1% v/v Triton-X 100, 1 mM MgCl2, 1X cOmplete EDTA-free protease inhibitor cocktail (Sigma), 0.1 % v/v Benzonase (Merck) ...
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bioRxiv - Neuroscience 2024Quote: Acutely isolated microglia were lysed in lysis buffer (50mM Tris, 150mM NaCl, 0.2% Triton X-100) containing protease inhibitors (cOmplete Mini, Sigma Aldrich). Cells were completely lysed by passing the lysates through a 25G needle ten times followed by incubation on ice for 10 min ...
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bioRxiv - Neuroscience 2024Quote: ... 150 mM NaCl, 1% sodium dodecyl sulfate [SDS], 0.5% sodium deoxycholate, 1% Triton X-100, 1x protease inhibitor cocktail [Sigma-Aldrich] ...
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bioRxiv - Microbiology 2024Quote: ... pH 7.0) containing the cOmpleteTM EDTA-Free protease inhibitor at a 1 x concentration following manufactureŕs instructions (Merck Millipore, Darmstadt, Germany). The buffer volume was proportional to the cell density of the sample ...
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bioRxiv - Biochemistry 2024Quote: ... Cells were harvested and pelleted before being suspended in a formaldehyde-reversal lysis buffer containing 500mM Tris + 150mM NaCl + 1% Triton X-100 + 2% SDS + 50mM 1,4-dithiothreitol (DTT) + 1X cOmplete EDTA-free Protease inhibitor cocktail (Sigma-Aldrich), pH 8 ...
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bioRxiv - Biochemistry 2024Quote: Cells were harvested and resuspended in lysis buffer (40 mM HEPES pH 7.5, 10 mM NaCl, 1% Triton X-100, 1X protease inhibitor cocktail (Sigma P2714), 20 mM β-glycerophosphate ...
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bioRxiv - Molecular Biology 2023Quote: ... then lysed with a solution of 0.015 % saponin in 1 X PBS with protease inhibitors (Millipore Sigma Cat. No. 11873580001) Parasites were pelleted and dissolved in sample buffer (Cell Signaling Technologies Cat ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5% Triton-X 100 and complete protease inhibitor) and co-purified by Anti-FLAG® M2 Magnetic Beads (Sigma-Aldrich). Briefly ...
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bioRxiv - Microbiology 2023Quote: ... Cells were grown and prepared as before to Queen buffer (50 mM HEPES, 200 mM KCl, 1 mM MgCl2, 0.05% Triton X-100, protease inhibitor cocktail (Sigma-Aldrich)) adjusted to pH 8.13 ...
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bioRxiv - Cancer Biology 2023Quote: Gastrocnemius muscle samples were lysed in lysis buffer (10% N-Deoxycholate, 0.1% SDS, 1% Triton X-100, 10 mM Tris pH 8, 140 mM NaCl, phosphatases/proteases inhibitors [Sigma-Aldrich] ...
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bioRxiv - Immunology 2023Quote: ... cells were lysed with chilled RIPA buffer (1% Triton X-100, 140mM NaCl, 50mM Hepes, 10mM Idoacetamide, 1×MS-SAFE Protease and Phosphatase Inhibitor (Sigma), PH7.4) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Each sample was pipetted immediately into 1.5 ml Eppendorf tubes containing 5 ul of 1 x Sigmafast Protease Inhibitor Cocktail (Sigma-Aldrich) on dry ice and subsequently stored at −80 °C until analysis.
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bioRxiv - Molecular Biology 2024Quote: ... The nematode pellet went 2x through the freeze/thaw cycle before sonication in M9 buffer containing 0.1% Triton X-100 and protease/phosphatase inhibitor mixture (Sigma-Aldrich). Whole-animal extract was centrifugation-cleared for 30 mins at 4°C ...
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bioRxiv - Neuroscience 2019Quote: ... permeabilized in 0.2% Triton-X (Sigma, X-100), and incubated for 48 hours in streptavidin conjugated Alexa Fluor 488 (1mg/1ml diluted to 1:1000 ...
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bioRxiv - Bioengineering 2019Quote: ... and 0.1% triton-X (Sigma, cat. #X-100) for one hour at room temperature ...
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bioRxiv - Genomics 2022Quote: ... Mouse gRNA constructs and pCMV-sadCas9-KRAB (kind gift from Dr. Alejandro Lomniczi OHSU) were co-transfected into human preadipocytes X-tremeGene (Sigma, 6366236001) while human gRNA plasmids and pCMV-sadCas9-KRAB were co-transfected into mouse 3T3-L1 preadipocytes ...