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Citations for Millipore Sigma :
601 - 650 of 3542 citations for TNFSF10 Human His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... aliquots of each fraction were visualized via immunoblotting (anti-His antibody; Cat: SAB2702218, Sigma Aldrich Canada, Oakville, Canada) to identify fractions enriched for RBD ...
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bioRxiv - Cell Biology 2022Quote: ... HCP-1 was produced similarly but in Hi-5 cells maintained in EX-Cell 405 medium (Sigma-Aldrich) and collected after 66h infection ...
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bioRxiv - Molecular Biology 2024Quote: ... The peak corresponding to mono-SPY-His-tagged streptavidin tetramer was collected and concentrated using Amicon 10K (Millipore). The concentrated mono-SPY-His6-tagged streptavidin tetramer was further purified through Hiload superdex75 (Cytiva ...
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bioRxiv - Bioengineering 2023Quote: ... His-tagged GA-MatryoshCaMP6s were eluted in 1.5 mL 250 mM imidazole (Sigma-Aldrich; CAS 1467-16-9) 20 mM MOPS pH 7.0 ...
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bioRxiv - Microbiology 2023Quote: ... The fractions containing His-SSRP1 were concentrated with an Amicon centrifugal filter unit (50 kDa cut-off, Millipore) and buffer exchanged into storage buffer (50 mM Tris-HCl pH 7.4 ...
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bioRxiv - Pathology 2023Quote: ... Then 2.4 µL of multiplexed PCR products were mixed with 9.35 µL of Hi-Di formamide (Sigma-Aldrich) and 0.15 µL of Genescan 500 Liz internal line size standard (Applied Biosystems ...
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bioRxiv - Microbiology 2023Quote: ... Fractions containing the His-tagged nucleotidases were pooled and concentrated using an Amicon 10 kDa MWCO filter (Millipore). The protein was buffer-exchanged into gel filtration buffer (100 mM HEPES pH 7.4 ...
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bioRxiv - Microbiology 2023Quote: ... Supernatant was isolated and combined with 2 ml of nickel-nitrilotriacetic acid (Ni-NTA) His Bind resin (Novagen), equilibrated in lysis buffer and incubated for 1 hour at 4°C with gentle rotation ...
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bioRxiv - Synthetic Biology 2023Quote: ... Membranes were then incubated with anti-His primary antibody 1:5000 in 4% milk in PBS-T (Sigma) for 2 hours ...
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bioRxiv - Biochemistry 2023Quote: ... The supernatant was flown through 500 μL of Roche cOmplete™ His-Tag purification resin (Millipore Sigma, #5893682001) for His-tag tagged proteins ...
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bioRxiv - Molecular Biology 2024Quote: ... 2 mM β-mercaptoethanol prior to application of the protein to His-Select Cobalt affinity gel (Sigma-Aldrich). The slurry was rotated for 60 min prior to application to a gravity flow column ...
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bioRxiv - Biochemistry 2024Quote: ... and lysates were clarified by ultracentrifugation (20,000 x g for 20 min) and incubated with 0.4 mL of His-Select nickel resin (Sigma) for 1 h at 4°C with gentle mixing ...
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bioRxiv - Cell Biology 2024Quote: ... the 6×His tag on the proteins was further cleaved with Thrombin CleanCleave kit (Sigma-Aldrich, Cat# RECMT) overnight at 4 °C on the rocking table after desaltig in 50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2024Quote: ... and the soluble fraction was purified by affinity chromatography (either Ni2+ or Strep) (His-Select resin (Sigma-Aldrich); Strep-Tactin®XT 4Flow resin (IBA Lifesciences)) ...
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bioRxiv - Biochemistry 2024Quote: Cleaved MntR was loaded onto a 5 ml column of Ni-NTA His•Bind® Resin (Millipore Sigma) equilibrated with Buffer A and then eluted with 40 mM imidazole ...
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bioRxiv - Bioengineering 2019Quote: ... Lyophilized proteins were purchased: human serum albumin (HSA; from human plasma, ≤0.02% Fatty acids, Lot #SLBZ2785, Millipore Sigma) and fibrinogen (FBG ...
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bioRxiv - Biochemistry 2021Quote: Lyophilized human haptoglobin phenotype 1-1 (Hp) and human α,β haemoglobin (Hb) were purchased from Sigma Aldrich. Deglycosylation enzyme PNgase F was from New England BioLabs Inc ...
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bioRxiv - Biophysics 2021Quote: PAI-1 (human recombinant, Sigma-Aldrich, Germany)
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bioRxiv - Biophysics 2021Quote: Fibrinogen (human plasma purified protein, Sigma-Aldrich)
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bioRxiv - Biophysics 2021Quote: ... PAI-1 (human recombinant, Sigma-Aldrich, Germany), Plasminogen (human plasma purified protein ...
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bioRxiv - Cell Biology 2019Quote: ... Recombinant human thrombin (1 U/ml, Sigma) was added to the bottom of a 3.0 µm costar polycarbonate transwell membrane insert (Corning ...
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bioRxiv - Cell Biology 2020Quote: ... sativa–derived recombinant human albumin (Sigma-Aldrich), and 213μg/ml L-ascorbic acid 2-phosphate (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... 10μM recombinant human Gastrin (Sigma-Aldrich G9145), 50ng/mL recombinant human HGF (Peprotech 100-39H) ...
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bioRxiv - Cell Biology 2019Quote: Primary human osteoblasts were purchased from Sigma. Co-culture cells were titrated to determine the highest viable seeding density to allow continuous culture for the duration of the osteoclast differentiation assay ...
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bioRxiv - Molecular Biology 2021Quote: ... 25 IU human chorionic gonadotropin (hCG, Sigma) was injected 76 h later (14:00 on day 4 of estrous cycle ...
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bioRxiv - Molecular Biology 2021Quote: ... 10 nM human gastrin I (Sigma-Aldrich), 500 nM A 83-01 (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2022Quote: ... coated with human plasma fibronectin (Millipore Sigma) diluted to 100 μg/mL in PBS ...
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bioRxiv - Bioengineering 2019Quote: ... Human B-lymphoid (Sigma-Aldrich, Buchs Switzerland), 293T Flp-in T-REX (Life Technologies ...
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bioRxiv - Immunology 2019Quote: ... 10% human serum (Sigma, St. Louis, MO), 10% heat-inactivated fetal bovine serum ...
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bioRxiv - Developmental Biology 2020Quote: ... and human chorionic gonadotropin (hCG) (Sigma Aldrich). Fertilized oocytes were then transferred into M2 medium (Millipore ...
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bioRxiv - Bioengineering 2019Quote: ... human tenascin-C (Millipore Sigma, Catalog #CC065), and human plasma fibronectin (Millipore Sigma ...
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bioRxiv - Developmental Biology 2019Quote: ... 10.7 μg/mL recombinant human transferrin (Sigma), and 20μg/mL recombinant human insulin (Peprotech) ...
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bioRxiv - Cancer Biology 2019Quote: ... 5 µg/mL human transferrin (Sigma-Aldrich), 92 nM FeCl3 (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2019Quote: Recombinant human insulin (hINS) powder (Sigma Aldrich) was dissolved in 20 mM HCl (pH 2) ...
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bioRxiv - Developmental Biology 2019Quote: ... Human embryonic kidney (HEK) 293T cells (Sigma) were maintained in DMEM supplemented with 10% fetal bovine serum ...
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bioRxiv - Biochemistry 2020Quote: Human neuroblastoma SH-SY5Y cells (Sigma-Aldrich) were grown in a 1:1 mixture of minimal essential medium (HyClone ...
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bioRxiv - Cancer Biology 2019Quote: ... and Human Adipocyte Magnetic Bead Panel (Millipore), a Bio-Plex 200 plate reader and the Bio-Plex Manager 6.1 software (Bio-Rad Laboratories) ...
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bioRxiv - Neuroscience 2020Quote: ... goat anti-human IgG-peroxidase (Sigma-Aldrich), and mouse monoclonal anti-NeuN (clone A60 ...
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bioRxiv - Immunology 2020Quote: ... human fibrinogen (Sigma-Aldrich, St Louis, MO); CD11b-FITC ...
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bioRxiv - Neuroscience 2019Quote: ... recombinant human TNFα (30 ng/mL, Sigma), and C1q (400 ng/mL ...
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bioRxiv - Cancer Biology 2019Quote: ... After incubation with human serum (Sigma-Aldrich) for 15 min ...
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bioRxiv - Bioengineering 2019Quote: ... 12.5 mg human insulin (Sigma I-6634), 100 U/mL Penicillin and 10 µg/mL Streptomycin (GIBCO 15140-122)) ...
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bioRxiv - Cancer Biology 2019Quote: ... 5 μg/ml human insulin (Sigma-Aldrich) and 2 % vol/vol B27 (Life Technologies ...
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bioRxiv - Genetics 2019Quote: ... using mouse anti-human ZCWPW1 (SIGMA SAB1409478) and mouse anti-polyhistidine (SIGMA H1029) ...
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bioRxiv - Developmental Biology 2021Quote: Mouse monoclonal anti-human αTubulin (Sigma, T5168), 1:3000.
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bioRxiv - Biochemistry 2021Quote: ... and human chorionic gonadotrophin (hCG, EMD Millipore) (9) ...
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bioRxiv - Bioengineering 2020Quote: ... thrombin from human plasma (Millipore Sigma, 605190) was resuspended in cold calcium chloride solution (11.1 mM CaCl2 ...
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bioRxiv - Neuroscience 2020Quote: ... gamma-globulin (human blood, Millipore Sigma, #H7379).
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bioRxiv - Cancer Biology 2020Quote: ... apo-Transferrin (human) (Sigma cat no. T5391) 0.01 mg/mL ...
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bioRxiv - Cancer Biology 2020Quote: ... Recombinant Human Insulin (Sigma cat no. I9278) 0.02mg/mL ...