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Citations for Millipore Sigma :
551 - 600 of 3691 citations for CD40 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... human plasma (Sigma-Aldrich, #5051); Rhodamine-conjugated fibronectin (Cytoskeleton Inc. ...
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bioRxiv - Microbiology 2023Quote: ... 1% human insulin (Sigma-Aldrich), and penicillin (100 I.U./ml ...
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bioRxiv - Immunology 2023Quote: ... A human IgG (Sigma #I2511) standard curve (ten three-fold serial dilutions starting at 20 µg/ml ...
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bioRxiv - Microbiology 2023Quote: ... 1% human insulin (Sigma-Aldrich), and penicillin (100 I.U./ml ...
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bioRxiv - Immunology 2023Quote: ... 2% human serum (Sigma-Aldrich), 1% non-essential amino acids (ThermoFisher) ...
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bioRxiv - Cell Biology 2024Quote: ... 850 nM human insulin (Sigma), 500 nM IBMX (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2024Quote: ... 850 nM human insulin (Sigma), 500 nM 3-isobutyl-1-methylxanthine (IBMX ...
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bioRxiv - Bioengineering 2024Quote: ... Human thrombin (Sigma, #T6884-100UN) was reconstituted in sterile 0.9% saline supplemented with human serum albumin (0.1% w/v ...
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bioRxiv - Cancer Biology 2024Quote: ... thrombin (from human plasma, Sigma) and aprotinin (from bovine lung ...
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bioRxiv - Immunology 2024Quote: ... 10% human serum (Sigma-Aldrich), 100 U/ml Penicillin (Cellgro Technologies LLC) ...
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bioRxiv - Immunology 2024Quote: ... 5% human AB serum (Sigma), 500 IU/mL of IL-2 (Proleukin S ...
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bioRxiv - Molecular Biology 2024Quote: Recombinant human PDE8A (Sigma SRP0273) was incubated with 100 ng FAK tyrosine kinase (Promega #V1971 ...
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bioRxiv - Molecular Biology 2024Quote: Human serum (Sigma-Aldrich, H4522) was thawed on ice and centrifuged at 18,000 ×g for 10 min at 4 °C to remove lipids ...
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bioRxiv - Pathology 2020Quote: ... high-purity (>99%) human CRP obtained from human plasma (C4063; Sigma-Aldrich, Saint Louis, Missouri) was infused via the femoral vein after 45 minutes of ischemia with LAD ligation ...
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bioRxiv - Biophysics 2020Quote: ... Human Serum from human male AB plasma and NaOH 1N Bioreagent were purchased from Sigma. ss M13mp18 ...
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bioRxiv - Immunology 2023Quote: ... Human platelets were stimulated with 1 U ml−1 Thrombin from human plasma (Sigma-Aldrich) or left unstimulated for 30 min to assess the purity and activation of isolated cells ...
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bioRxiv - Biochemistry 2024Quote: ... and human apo-transferrin (P02787) isolated from human plasma were purchased from Sigma Aldrich (E0518), Invitrogen (RP-43132) ...
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bioRxiv - Neuroscience 2021Quote: UBQLN2 complexes were isolated from HEK293 cells overexpressing FLAG-tagged wild-type UBQLN2 using anti-FLAG M2 Affinity Gel (Millipore-Sigma, A2220). After washing ...
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bioRxiv - Biochemistry 2022Quote: HEK293 cells were obtained from ATCC and were tested negative for mycoplasma using LookOut® PCR-based kit (MP0035-1KT Sigma-Aldrich). HEK293-Klotho cells stably expressing Klotho were a gift from Dr ...
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bioRxiv - Biochemistry 2023Quote: Plasmids of Cypridina noctiluca luciferase and Omicron RBD or its mutants were co-transfected (9:1) into HEK293 cells using X-tremeGENE 9 (Millipore-Sigma XTG9-RO). After 16 hours ...
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bioRxiv - Biochemistry 2024Quote: ... along with packaging vectors psPAX2 (#12260) and PMD2.G (#12259) were transfected into the HEK293-FT cells using XTremeGene 9 (Sigma Aldrich, 6365779001) (A375m ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant pH was adjusted to 8.0 and incubated with Ni2+-NTA His-binding resin (Novagen) that was pre-equilibrated with binding buffer (50 mM Tris-HCl ...
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bioRxiv - Biochemistry 2021Quote: ... the blot was incubated with a mouse anti-His-tag primary antibody (Sigma-Aldrich, Steinheim, Germany), diluted 1:1 000 in TBS (10 mM TRIS/HCl (pH 7.5) ...
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bioRxiv - Biochemistry 2020Quote: ... The Trx-His(6) tag was cleaved by addition of 300 units of thrombin (Sigma T4648) directly to the eluate and the solution dialyzed (Slide-A-Lyzer dialysis cassettes ...
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bioRxiv - Plant Biology 2020Quote: ... The His6-SEC5B fusion protein was extracted and purified with Ni-NTA His bind resin (Novagen). The freshly extracted resin-bound GST or GST-XIK proteins were mixed with purified His6-SEC5B protein in protein interaction buffer and incubated at 4°C for 2 h with shaking ...
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bioRxiv - Biophysics 2020Quote: ... MBP and the His-tag were cleaved from the P domain using HRV 3C protease (Novagen). Cleaved P domain protein eluted from Ni-NTA resin and was further purified by size-exclusion chromatography using a Superdex 16/600 200 pg column (GE Healthcare ...
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bioRxiv - Neuroscience 2019Quote: ... or combined together (GPA2-FLAG/GPB5-His) and then treated with DSS (Sigma Aldrich, Oakville, ON). In some experiments ...
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bioRxiv - Immunology 2021Quote: ... using cross-reactive anti-SARS-CoV-1 Spike CR3022 or mouse anti-His tag (Sigma-Aldrich) as positive controls ...
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bioRxiv - Neuroscience 2019Quote: FXR1(1-379) was expressed as His- tagged fusion protein in Rosetta (DE3) pLysS cells (Novagen) in LB medium ...
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bioRxiv - Plant Biology 2021Quote: ... and GST were fused with a C-terminal octa-His tag in the pET42 vector (Novagen).
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bioRxiv - Biochemistry 2021Quote: ... The latter were then probed with mouse anti-His HRP conjugated antibody (1:400, Sigma-Aldrich) and visualized by chromogenic DAB reagent (Sigma).
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bioRxiv - Molecular Biology 2022Quote: ... Cleared supernatant was incubated with 20 μl His Mag Sepharose Ni beads (Sigma, GE28-9673-88) overnight ...
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bioRxiv - Physiology 2022Quote: ... The F4-2_Fab protein was first purified using cOmplete® His-tag purification resin (Sigma-Aldrich), and L6-F4-2 protein was first purified with CaptivA Protein A affinity resin (Repligen) ...
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bioRxiv - Neuroscience 2024Quote: ... and the supernatant subjected to Ni-NTA affinity purification (HIS-Select Nickel Affinity Gel (Sigma Aldrich)) ...
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bioRxiv - Biochemistry 2024Quote: ... The UbVs and Ube2d2S22R-Avi were purified by nickel-affinity chromatography with HIS-select resin (Sigma) followed by an overnight 3C digestion using in-house produced 3C protease ...
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bioRxiv - Biochemistry 2024Quote: ... and the protein solution was mixed with Co resin (His-select Cobalt Affinity Gel, Sigma Aldrich) for 1h at 4 °C ...
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bioRxiv - Biochemistry 2024Quote: ... The resin-bound His-tagged proteins were washed with wash buffer containing 20mM imidazole (Sigma #12399) to eliminate non- specific binding and eluted 5 times subsequently with elution buffer (20mM Tris HCl pH7.5 ...
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bioRxiv - Biochemistry 2023Quote: ... The lysate was clarified by centrifugation and applied to cOmplete His-tag purification resin (Sigma Aldrich) for 1 hour at 4 °C with rotation ...
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bioRxiv - Molecular Biology 2024Quote: ... Clarified lysate was applied to 2.5 ml bed volume His-select Cobalt Affinity Gel (Sigma-Aldrich). The slurry was rotated in the cold room for an hour prior to application to a gravity flow column ...
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bioRxiv - Cell Biology 2020Quote: The following antibodies were used procollagen VII (rabbit anti–human [Abcam]; mouse anti-human [Sigma-Aldrich]), Sec31A (mouse anti–human ...
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bioRxiv - Physiology 2019Quote: ... human tubal fluid (HTF) medium and human serum albumin (HSA) (Merck Millipore Corporation, Billerica, MA, USA), Fluo-4 AM and Pluronic F-127 (Molecular Probes ...
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bioRxiv - Pathology 2024Quote: ... RNA from human liver tissue and human cirrhotic PCLS was isolated using Tri Reagent (T9424, Sigma) and 1-bromo-3-chloropropane (B9673 ...
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bioRxiv - Cancer Biology 2021Quote: ... 5□µg/ml human insulin (Sigma), 100 U/ml penicillin and 100□μg/ml streptomycin (Gibco ...
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bioRxiv - Neuroscience 2020Quote: ... human epidermal growth factor (hEGF; Sigma), recombinant human basic fibroblast growth factor (bFGF ...
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bioRxiv - Immunology 2021Quote: ... Commercially available human serum (Sigma-Aldrich) was used as the positive control.
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bioRxiv - Biochemistry 2020Quote: ... human liver cathepsin B (Millipore Sigma), and human cathepsin K (Enzo Sciences ...
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bioRxiv - Developmental Biology 2020Quote: ... mouse anti-human nuclei (Millipore MAB1281), mouse anti-TUJ1 (Covance MMS 435P) ...
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bioRxiv - Developmental Biology 2020Quote: ... of human chorionic gonadotrophin (hCG) (Sigma) 46 hours later ...
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bioRxiv - Developmental Biology 2020Quote: ... human insulin was purchased by SIGMA and was used at a final concentration of 7ug/ml ...
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bioRxiv - Cell Biology 2022Quote: ... supplemented with human albumin (Sigma, #A0237), sodium DL-lactate (Sigma ...