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Citations for Millipore Sigma :
551 - 600 of 1467 citations for African Swine Fever virus pp62 polyprotein His tag since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... we filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1.5 mL aliquots in cryovials at -80ºC.
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bioRxiv - Systems Biology 2023Quote: ... we filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1.5 mL aliquots in cryovials at -80ºC.
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bioRxiv - Neuroscience 2023Quote: ... cells were lysed and virus was concentrated using an Optiprep density gradient (Sigma #D1556). Small-scale AAV virus was produced in HEK293T cells grown in 12-well plates by triple-transfection with 0.4μg HiUGE vector ...
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bioRxiv - Bioengineering 2023Quote: ... we filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1.5mL aliquots in cryovials at -80°C.
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bioRxiv - Microbiology 2023Quote: ... and resuspending in virus + polybrene (final conc 8 ug/mL, Sigma-Aldrich, TR-1003) or RPMI media + polybrene for the uninfected control at a concentration of 1E6 cells/mL ...
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bioRxiv - Cancer Biology 2023Quote: ... the virus-containing supernatants from HEK293 cultures mixed with polybrene (Sigma, St. Louis, MO) at a final concentration of 8 mg mL-1 were added to the cells in each well ...
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bioRxiv - Bioengineering 2023Quote: ... we filtered the virus-laden media through a 0.45µm PES filter (Millipore-Sigma #SE1M003M00) and stored 1.5mL aliquots in cryovials at -80°C.
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bioRxiv - Cancer Biology 2023Quote: ... Virus (400 µL) was added together with 5 ug/mL of polybrene (Sigma-Aldrich) and cells were centrifuged at 2,000 rpm for 2 h at 30°C ...
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bioRxiv - Molecular Biology 2021Quote: ... M2 anti-FLAG tag (1:1000, from 1 mg mL−1, Sigma), anti-β-actin (1:5000 ...
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bioRxiv - Microbiology 2021Quote: ... The C-terminal twin-Strep-Tag was removed by HRV3C (Sigma Aldrich) digestion overnight at 4 °C and the uncleaved protein was removed by passage over Ni-NTA resin ...
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bioRxiv - Microbiology 2020Quote: ... Immunoblots were performed with anti-GFP tag primary monoclonal antibody (Sigma-Aldrich) as described by50.
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bioRxiv - Microbiology 2021Quote: ... and the antibody for the FLAG tag was from Sigma (cat # F7425).
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bioRxiv - Plant Biology 2021Quote: ... or western blot using HA Tag Antibodies (Sigma-Aldrich, St. Louis, MO).
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bioRxiv - Molecular Biology 2022Quote: ... HA tag antibody (1:1200, cat# 04-902; EMD Millipore, clone DW2). Blots incubated overnight at 4°C ...
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bioRxiv - Biochemistry 2020Quote: ... and cloned into PET-24a (+) C-terminal-histidine-tag vector (Novagen, Germany), which was digested by XhoI and NdeI and dephosphorylated by Antarctic Phosphatase (New England BioLabs ...
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bioRxiv - Molecular Biology 2020Quote: ... but with removal of the His6 tag was by a thrombin (Sigma) protease cleavage step before concentration to 40 mg/ml.
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bioRxiv - Physiology 2020Quote: ... Ptch1 was immunoprecipitated with anti myc-tag antibodies (Millipore, Cat# 05-724). Expression of Dhh ...
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bioRxiv - Biochemistry 2021Quote: ... monodisperse tag-free Ape1 was concentrated using 10 kDa MWCO Amicon (Millipore) to 15–20 mg/ml ...
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bioRxiv - Synthetic Biology 2021Quote: Recombinant hACE2 with a C-terminal deca-histidine tag (SAE0064, Sigma-Aldrich) was resuspended in PBS to a final concentration of 50 ng/μL ...
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bioRxiv - Immunology 2021Quote: ... Antibodies to Anti-Flag-tag (F1804) for immunoprecipitation were obtained from SIGMA. Antibodies to Ub (ab7780) ...
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bioRxiv - Microbiology 2021Quote: ... cells were incubated with an anti-HA epitope tag PAb (Sigma, H6908) and then with a FITC-conjugated donkey anti-rabbit secondary Ab (Invitrogen) ...
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bioRxiv - Cancer Biology 2022Quote: ... horseradish peroxidase (HRP)-conjugated mouse anti-FLAG tag antibody (A8592, Sigma-Aldrich) was used to detect VH binding ...
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bioRxiv - Plant Biology 2022Quote: ... Other primary antibodies we employed were: anti-HA tag antibody (Sigma, H6908); anti-c-Myc tag antibody (Abcam ...
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bioRxiv - Cell Biology 2024Quote: ... PLK1 rabbit (used in Phos-tag gel and PLK1 IP, Millipore ABE2619), phospho-PRC1 T481 (Abcam ab62366) ...
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bioRxiv - Cell Biology 2024Quote: ... Strep-Tag II Antibody HRP Conjugate (Sigma Aldrich-71591-3, 1:2000). Rabbit anti-K8.1 (gift of Denise Whitby ...
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bioRxiv - Molecular Biology 2023Quote: ... Antibodies for FLAG- tag (Cat # F3165, RRID:AB_259529) was obtained from Sigma-Aldrich. KDM2A (Cat # ab191387 ...
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bioRxiv - Cell Biology 2023Quote: ... Triacylglycerol (TAG, Cat# T7140) and monoacylglycerol (MAG, Cat# M2015) were from Sigma. β-Glucosylceramide (β-GlcCer ...
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bioRxiv - Cell Biology 2022Quote: ... The following rabbit antibodies were used: HA-tag (Sigma-Aldrich H6908; RRID:AB_260070); LAMP1 (Cell Signalling D2D11 ...
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bioRxiv - Molecular Biology 2022Quote: ... Membranes were incubated with monoclonal anti-(c-Myc Tag) antibody (Merck Millipore) (HeLa extracts) ...
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bioRxiv - Cell Biology 2023Quote: ... M2 anti-FLAG tag (1:1000, from 1 mg mL−1, Sigma), anti-β-actin (1:5000 ...
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bioRxiv - Biophysics 2023Quote: ... containing a C-terminal EmeraldFP–6xHIS tag was cloned into pET22b (Novagen) for expression in E ...
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bioRxiv - Biochemistry 2023Quote: ... S-tag was removed with Factor Xa Cleavage Capture kit (Millipore Sigma). The untagged sF or sG was further purified with size-exclusion column (HiLoad 16/60 Superdex 200 prep grade XK 16 gel filtration column (GE Healthcare) ...
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bioRxiv - Microbiology 2023Quote: ... and Trx tags derived from the pET-32a/pET28 cloning vector (Novagen) from ClearColi (Lucigen ...
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bioRxiv - Cell Biology 2023Quote: ... anti-myc tag (Sigma-Aldrich, M4439; IB, 1:10,000; IP, 1 μg;), anti-AP3B1 (Bethyl labs 50-157-0195 ...
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bioRxiv - Plant Biology 2019Quote: ... Immuno-blotting was performed using anti-ubiquitin antibody and anti-His (Sigma, USA).
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bioRxiv - Biochemistry 2020Quote: 25 μl of the monoclonal α-His antibody (Catalog-ID H1029, Sigma-Aldrich) were mixed with 100 μl buffer comprising 50 mM HEPES-NaOH ...
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bioRxiv - Biochemistry 2020Quote: ... The Gag-His6 protein was loaded onto a HIS-select affinity column (Sigma), washed (20 mM Tris-HCl ...
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bioRxiv - Biophysics 2021Quote: His-tagged human PCNA protein was purchased from Sigma Aldrich (catalogue number SRP5117), at a concentration of 6.6 μM ...
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bioRxiv - Cell Biology 2021Quote: ... SC –leu-trp plates and SC –leu-trp-his + 10mM 3-aminotriazole (Sigma) plates ...
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bioRxiv - Neuroscience 2020Quote: ... and HIS dihydrochloride (Sigma-Aldrich; 10 μg/10 μl; 9 mM; 90 nmol), as well as a combination of 2 substances (ALA 90 μg with BAM8-22 1 μg in 10 μl ...
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bioRxiv - Biochemistry 2022Quote: ... S5H recombinant protein was examined by using specific anti-His mouse antibody (Novagen) as described in (López-Gresa et al. ...
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The formation of a fuzzy complex in the negative arm regulates the robustness of the circadian clockbioRxiv - Molecular Biology 2022Quote: ... and Western blotting with primary anti-His at 1:1000 (Sigma Aldrich, SAB1305538), secondary anti-Mouse at 1:10000 (ThermoFisher ...
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bioRxiv - Molecular Biology 2022Quote: ... which coeluted with His-TrxA or monoclonal anti-polyHistidine-Peroxidase antibody (Sigma-Aldrich) for the detection of recombinant His-tagged TrxA ...
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bioRxiv - Bioengineering 2022Quote: ... Cells were washed with a washing solution of 4% HI-FBS (#F4135; Sigma) in PBS and permeabilized with 0.3% Triton X-100 (#T8787 ...
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bioRxiv - Synthetic Biology 2021Quote: ... and Guanidine Hydrochloride (Serva, 24205.02) via Hi-Trap Desalting column (Sigma Aldrich GE17140801) for further experiments ...
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bioRxiv - Biochemistry 2021Quote: ... The supernatant was incubated with 15 ml HIS-Select Nickel Affinity Gel (Sigma) for 1 h at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Fractions containing His-Pap1 were pooled and concentrated using 30,000 MWCO concentrators (Millipore).
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bioRxiv - Biochemistry 2022Quote: ... cleared cell lysates were captured by Ni-NTA His-bind resin (Merk Millipore) equilibrated in high salt buffer (50 mM Tris pH8 ...
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bioRxiv - Molecular Biology 2022Quote: These His-tagged protein lysates were combined with 1 ml nickel beads (Sigma) and incubated at 4 °C/2 hours/rolling ...
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bioRxiv - Microbiology 2022Quote: ... purified from bacterial lysates on a nickel affinity resin (His-Select, Sigma-Aldrich) as described (12) ...