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Citations for SignalChem :
1 - 44 of 44 citations for Recombinant Human Endothelin Converting Enzyme 1 His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: Recombinant GST-tagged LTK (SignalChem #L11-11G) was incubated in the absence or in the presence of an equal amount of recombinant His-tagged IGF-1R (SignalChem #I02-11H ...
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bioRxiv - Neuroscience 2023Quote: ... was incubated in the absence or in the presence of an equal amount of recombinant His-tagged IGF-1R (SignalChem #I02-11H) in kinase buffer (50 mM HEPES ...
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bioRxiv - Cell Biology 2022Quote: ... human recombinant GST-fusion ERK3 protein (SignalChem) and E.coli purified ARP3 ...
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bioRxiv - Neuroscience 2023Quote: ... Immobilized recombinant proteins were then incubated with 400 µl embryonic brain homogenates or with recombinant His-IGF-1R (SignalChem #I02-11H) for 2 h ...
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bioRxiv - Biochemistry 2023Quote: ... or recombinant human IKKβ protein (Signalchem, I03-10BG-10) in kinase reaction buffer in 96-well plates ...
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bioRxiv - Biochemistry 2023Quote: ... and recombinant human STK25 protein (Signalchem, S43-10G-10) or recombinant human IKKβ protein (Signalchem ...
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bioRxiv - Neuroscience 2023Quote: ... Other human recombinant PKCs (SignalChem Biotech, Richmond, BC, Canada) were assayed in triplicate at the following concentrations ...
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bioRxiv - Biochemistry 2020Quote: Human RIPK1 full length GST-tagged was purchased from SignalChem (Richmond, CA, USA). The protocol used to detect the enzymatic activity was described in Delehouzé et al17 ...
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bioRxiv - Biochemistry 2023Quote: ... was incubated with recombinant human STK25 protein (Signalchem, S43-10G-10) (350 ng ...
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bioRxiv - Cancer Biology 2024Quote: ... Protein kinase activity of recombinant full-length AMPK (A1/B1/G1) enzyme (SignalChem, Richmond, BC Canada) was determined using a non-radioactive assay which measure changes in initial concentration of ATP via luminescence ...
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bioRxiv - Biochemistry 2019Quote: ... the 5 uL reaction was performed with 4 ug/mL recombinant human ULK2 protein (1-478, SignalChem #U02-11G) and 80 ug/mL MBP in the presence of 25 uM ATP ...
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bioRxiv - Biochemistry 2019Quote: ... the 5 uL kinase reaction was performed with 2 ug/mL recombinant human ULK1 protein (1-649, SignalChem #U01-11G) and 80 ug/mL myelin basic protein (MBP ...
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bioRxiv - Neuroscience 2019Quote: Recombinant full length human PFKFB3 protein purified from Sf9 baculoviral system acquired from SignalChem (Cat. #P323-30G). ATP ...
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bioRxiv - Neuroscience 2020Quote: ... with 1 μg tau 441 purified recombinant proteins (SignalChem, T08-55BN-20) in kinase assay buffer (25 mM MOPS ...
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bioRxiv - Neuroscience 2023Quote: ... recombinant GST (SignalChem #G52-30U) and GST-LTK (SignalChem #L11-11G ...
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bioRxiv - Molecular Biology 2020Quote: ... recombinant-activated RSK1 purchased from SignalChem was used with bacterially purified recombinant GST-RIOK2 (aa 443–552 ...
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bioRxiv - Neuroscience 2019Quote: 200 ng of recombinant HRI (SignalChem) or 500ng of recombinant eIF2a (abcam ...
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bioRxiv - Cancer Biology 2022Quote: ... were incubated with 1 μg commercially available human IKKα protein (SignalChem) for 30 minutes at 30°C ...
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bioRxiv - Cell Biology 2021Quote: ... 50 ng of purified recombinant active MK5 (SignalChem) was incubated with 15 µg of purified recombinant FoxO3 in 50 µl of phosphorylation buffer (40 mM Tris-HCl (pH 7.5) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and the corresponding His tag (R06-32BH) were obtained from SignalChem Lifesciences (Richmond ...
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bioRxiv - Neuroscience 2022Quote: ... Full-length recombinant Tau 1-441 phosphorylated in vitro by glycogen synthase kinase 3β (GSK3β) (SignalChem, Vancouver, BC, Canada) was used as the calibrator.
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bioRxiv - Molecular Biology 2021Quote: ... and 300 ng of recombinant active p38α kinase (SignalChem) were mixed in pre-chilled 1.5 mL tubes ...
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bioRxiv - Cancer Biology 2022Quote: ... or ROCK2 enzyme (0.006 ng/mL; SignalChem, #R11-11H-10) was added to each well of a 384-well plate containing 1 μM Kemptide peptide substrate (5-FAM-LRRASLG ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.1 μg of Recombinant Active TBK1 (T02-10G-05, SignalChem), and 1 μL 10 mM ATP were combined in kinase reaction buffer (20 mmol/L Tris-HCl pH 7.4 ...
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bioRxiv - Molecular Biology 2022Quote: ... recombinant Cyclin B1/Cdk1 (SignalChem Biotech Inc., Richmond, BC, Canada), and ADP-Glo kinase assay (Promega) ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... Active recombinant BTK was purchased from SignalChem (Cat.# B10-10H-10). All the drugs (3 warhead control and 4 PROTACs ...
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bioRxiv - Physiology 2020Quote: ... Recombinant SGK1 and GSK-3ß were purchased from SignalChem (Richmond, Canada).
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bioRxiv - Cell Biology 2019Quote: ... recombinant fragments of MBP-Megator were incubated with 0.05μg HsMps1/TTK (SignalChem, Richmond, Canada) in a total volume of 30μl kinase reaction buffer (5 mM MOPS pH 7.2 ...
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bioRxiv - Biochemistry 2023Quote: ... 2 mM ATP) and 2 ug of recombinant kinase (SignalChem; Tyro3 (aa 455-end): T22-11G ...
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bioRxiv - Cancer Biology 2022Quote: Human DARPP-32 isoforms purified from NSCLC cells were incubated with kinase-activated human IKKα protein (SignalChem) for in vitro kinase assays by following previously described methods70 ...
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bioRxiv - Cell Biology 2022Quote: ... we performed in vitro kinase assaay using recombinant MK5 (MAPKAPK5 Unactive Cat# M42-14G, SignalChem) and Myelin basic protien (MBP ...
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bioRxiv - Cell Biology 2022Quote: In order to asses kinase activity of full-lenght (recombinant ERK3 Cat# M31-34G, SignalChem) and kinase domain ERK3 (ERK3 (amino acids (aa ...
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bioRxiv - Cancer Biology 2024Quote: ... Enzyme kinase activity was measure in the presence of 45 µM peptide substrate SAMStide (HMRSAMSGLHLVKRR) (SignalChem) in a buffered solution containing 5 mM MOP (pH 7.5) ...
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bioRxiv - Microbiology 2020Quote: ... All the recombinant kinases (SRPK1/2/3, GSK-3α/β and CK1A/E were obtained from SignalChem.
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bioRxiv - Neuroscience 2023Quote: ... In vitro kinase assays were performed using purified recombinant Cpx proteins and the catalytic subunit of CK2 (C70-10G, SignalChem). Briefly ...
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bioRxiv - Cancer Biology 2021Quote: ... 500 nM (170 nM was used on some repeats to conserve material) of recombinant glutathione S-transferease (GST)-CRAF (Y340E/Y341E) kinase domain (sequence: 306-end) (SignalChem, cat# R01-13G); His-BRAF kinase domain (sequence ...
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bioRxiv - Developmental Biology 2021Quote: ... and anti-mouse (G32-62G, 1:10,000, SignalChem) horseradish peroxidase-conjugated antibodies at room temperature for 1 hr before exposure on photographic film (28906844 ...
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bioRxiv - Microbiology 2020Quote: ... and/or CK1ε (30 nM) in Kinase Buffer 1 (SignalChem) for 15 minutes at 30 degrees C ...
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bioRxiv - Cancer Biology 2020Quote: ... and anti-mouse HRP (G32-62G-1000) 1:10,000 (SignalChem). Membranes were developed with Supersignal™ West Pico PLUS Chemiluminescent Substrate (Pierce ...
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bioRxiv - Physiology 2022Quote: ... 0.1 mg/mL of BSA and 1× Assay Buffer I (SignalChem) were added to liposomes and 50 μL volumes were distributed across three Eppendorf tubes ...
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bioRxiv - Physiology 2022Quote: ... the beads (50 μL) were incubated in 1× Assay Buffer I (SignalChem), 0.5 μL ATP (10 mM) ...
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bioRxiv - Developmental Biology 2021Quote: ... blots were incubated with secondary anti-rabbit (G33-62G, 1:10,000, SignalChem, Canada) and anti-mouse (G32-62G ...
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bioRxiv - Physiology 2022Quote: ... and the lipid pellet was resuspended in 50 μL 1× Assay Buffer I (SignalChem), vigorously until resuspended ...
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bioRxiv - Neuroscience 2024Quote: ... 0.1 μg/ml MEK1 activated extracellular signal-regulated kinase 1 and 2 (pERK1/2) (SignalChem Biotech), 1 μM TTA-P2 (Alomone Labs) ...