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Citations for Addgene :
1 - 50 of 1322 citations for rno mir 22 Real Time RT PCR Detection and U6 Calibration Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... U6-1 and U6-3 (Addgene plasmid #83954 ...
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bioRxiv - Neuroscience 2023Quote: ... U6:sgRNA1;U6:sgRNA272 (Addgene 74009) as a backbone and performed the following modifications ...
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bioRxiv - Genetics 2022Quote: ... were cloned into pCFD4-U6:1-U6:3 (Addgene® plasmid #49411 ...
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bioRxiv - Neuroscience 2023Quote: ... The pUAS:Cas9T2ACre;U6:sgRNA1;U6:sgRNA2 plasmid (Addgene #74010) was digested using BsaI (R0535S ...
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bioRxiv - Cell Biology 2020Quote: ... primary miRNAs for miR-29a and miR-16 were amplified from genomic DNA by PCR and cloned into the pAdTrack shuttle (Addgene, Plasmid#16404). The miR-16 pAdTrack plasmid was then mutated by site-directed mutagenesis to induce 3 point mutations into the miR-16 seed sequence ...
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bioRxiv - Genetics 2020Quote: ... The U6:3 promoter was PCR amplified from pAC-U63-tgRNA-Rev (Addgene #112811). The CR7T promoter was synthesized as a gBlock DNA fragment (IDT ...
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bioRxiv - Cell Biology 2020Quote: ... pUAS:Cas9T2AGFP;U6:sgRNA1;U6:sgRNA2 was a gift from Filippo Del Bene (Addgene plasmid #74009 ...
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bioRxiv - Cell Biology 2020Quote: ... vectors pBS-U6-EGFP and pBS-U6-DsRed were generated by subcloning the murine U6 promoter from pCS6/U6 (a gift from Connie Cepko (Addgene plasmid # 15173) (Harpavat & Cepko ...
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bioRxiv - Synthetic Biology 2021Quote: ... pCR-U6-gRNA-miniCMV-TdTomato was generated by inserting miniCMV-TdTomato from reporter-gT1 (Addgene plasmid #47320 ...
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bioRxiv - Cancer Biology 2019Quote: pUC57-U6-sgRNA (#51132, Addgene); Cas9-VP12 (#72247 ...
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bioRxiv - Bioengineering 2023Quote: ... miR-92a (Addgene #46672), let-7a (Addgene #51377 ...
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bioRxiv - Cell Biology 2020Quote: ... PCR amplified and cloned between SalI and XbaI sites of pAV-U6+27 (Addgene, plasmid #25709) to yield pAV-U6+27-RhoBAST2 ...
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bioRxiv - Neuroscience 2021Quote: ... the U6-BbsI/BbsI cassette was PCR amplified from px458 (Addgene #48138, (Ran et al., 2013)) (forward ...
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bioRxiv - Neuroscience 2023Quote: ... Both PCR products were assembled with the Tol2 Gateway plasmid backbone (pDestTol2pA2-U6:gRNA; Addgene #63157) using Hi-Fi DNA Assembly to obtain elavl3:hArr-TEV;he1.1:YFP.
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bioRxiv - Neuroscience 2022Quote: ... Viral vectors were generated from Addgene plasmids #20297 and #50459 and titers determined via fluorometric quantification (Neuroscience Center Zurich) or by real-time quantitative PCR combined with SYBR green technology (Addgene). Respective titers were >9.1×1012 vector genomes/ml (Neuroscience Center Zurich ...
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bioRxiv - Genetics 2019Quote: pX330-U6-Chimeric vector (Addgene, #42230) and lentiCRISPR v2 plasmidwith puromycin-resistance (Addgene ...
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bioRxiv - Genetics 2022Quote: ... U6: BsaI-sgRNA (pX601, #61591, Addgene). The CMV enhancer promoter was replaced by a commonly used short ...
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bioRxiv - Cancer Biology 2023Quote: ... LRG2.1 (U6-sgRNA-GFP, Addgene, #108098) or LRPuro (U6-sgRNA-Puromycin ...
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bioRxiv - Bioengineering 2022Quote: ... Both U6-gRNA-scaffold sequences were cloned into a px330-U6-Chimeric_BB-CBh-hSpCas9 plasmid2 (Addgene #42230) (pX330-2gRNAs) ...
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bioRxiv - Plant Biology 2023Quote: ... Each gRNA PCR fragment was individually assembled with Arabidopsis U6 small RNA promoter (pICSL01009::AtU6p, AddGene no. 46968) in the appropriate Level 1 vector (plCH47751 ...
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bioRxiv - Microbiology 2021Quote: ... to human CD59 or CD46 were cloned into a pX330-U6-Chimeric_BB-CBh-hSpCas9 plasmid backbone (pX330-U6-Chimeric_BB-CBh-hSpCas9 was a gift from Feng Zhang (Addgene plasmid # 42230 ...
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bioRxiv - Immunology 2020Quote: ... (pX330-U6-Chimeric_BB-CBh-hSpCas9, Addgene #42230). sgRNA oligonucleotides were ordered from Integrated DNA Technologies (IDT) ...
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bioRxiv - Genomics 2022Quote: ... and 10µg U6-B2M (Addgene plasmid #171635) using polyethylenimine (Polysciences Inc. ...
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bioRxiv - Molecular Biology 2022Quote: pX330-U6-Chimeric_BB-CBh-hSpCas9 (Addgene #42230)6 ...
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bioRxiv - Cell Biology 2023Quote: ... a pShuttle-U6 vector (Addgene plasmid #13428) was modified by replacing the U6 promoter and multiple cloning site (MCS ...
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bioRxiv - Cell Biology 2023Quote: ... (pX330-U6-Chimeric_BB-CBh-hSpCas9, Addgene #42230). sgRNA oligonucleotides were ordered from Integrated DNA Technologies (IDT) ...
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bioRxiv - Cancer Biology 2023Quote: pSIN-Clover-Puro and pSIN-Clover-miR-497-Puro were generated from the modified pSIN-Puro backbone utilizing pre-miR-497 cloned from the pMSCV-PIG-miR-497/miR-195 (Addgene #64236, a gift from Joshua Mendel17). Doxycycline-inducible shRNAs targeting Renilla and Vat1 were generated in LT3GEPIR61 (gift from Johannes Zuber ...
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bioRxiv - Cancer Biology 2020Quote: pUltra-U6-crRNAs-U6-tracr was constructed in a 3-part Gibson assembly using PacI linearized pUltra (Addgene #24129)54 backbone ...
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Mitigating a TDP-43 proteinopathy by targeting ataxin-2 using RNA-targeting CRISPR effector proteinsbioRxiv - Bioengineering 2023Quote: ... PCR was used to amplify the U6-crRNA scaffold and the DiCas7-11 gene sequence from pDF0114 (Addgene #172508) and pDF0159 (Addgene #172507) ...
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bioRxiv - Molecular Biology 2021Quote: ... and gGene-N2a and gGene-N2b for Nickase 2) cloned into tandem U6 cassettes in a version of pX330 plasmid (pX330-U6-Chimeric_BB-CBh-hSpCas9, Addgene #42230) mutated to express Cas9D10A-T2A-GFP (Nickase-1/2) ...
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bioRxiv - Molecular Biology 2021Quote: ... were cloned into tandem U6 cassettes in a variant of the original pX330 plasmid (pX330-U6-Chimeric_BB-CBh-hSpCas9, Addgene #42230) modified to express Cas9WT-T2A-GFP (pX330-Cas9WT-T2A-GFP ...
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bioRxiv - Microbiology 2021Quote: A doxycycline-inducible CRISPR/Cas9 expression vector (pSBtet-puro-Cas9-U6) was generated by cloning the Cas9-U6 portion of pX459 (Addgene #62988 ...
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bioRxiv - Immunology 2020Quote: ... 3 gRNAs against Pdap1 gene were cloned into the U6 cassette of a variant of the original pX330 plasmid (pX330-U6-Chimeric_BB-CBh-hSpCas9, Addgene #42230) modified to express Cas9WT-T2A-GFP (kind gift from Van Trung Chu ...
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bioRxiv - Immunology 2020Quote: ... SpCas9 sgRNA was expressed using pSL21-VEX or pSL21-mCherry (U6-sgRNA-EFS-VEX or U6-sgRNA-EFS-mCherry, will be available through Addgene). To generate the pSL21-VEX or pSL21-mCherry ...
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bioRxiv - Neuroscience 2023Quote: ... This allows the removal of tandem U6 promoters with sgRNAs and insertion into AAV-U6-sgRNA-hSyn-mCherry (Addgene #87916) in place of the single U6-sgRNA when digested with MluI and ApaI ...
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bioRxiv - Microbiology 2024Quote: A doxycycline-inducible CRISPR/Cas9 expression vector (pSBtet-puro-Cas9-U6) was generated by cloning the Cas9-U6 portion of pX459 (Addgene #62988 ...
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bioRxiv - Cell Biology 2020Quote: ... a derivative of pCFD3:U6:3gRNA (Addgene, #49410) for expression of a single guide RNA ...
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bioRxiv - Cell Biology 2020Quote: ... pX335-U6-Chimeric_BBCBh-hSpCas9n (pX335) (Addgene plasmid #42335) encoding the SpCas9 D10A nickase ...
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bioRxiv - Neuroscience 2020Quote: ... pX330-U6-Chimeric_BB-CBh-hSpCas9 (Addgene, ID #42230), coding for the nuclease Cas9 and the chimeric guide RNA (gRNA) ...
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bioRxiv - Molecular Biology 2022Quote: ... or pX330-U6-Chimeric_BB-CBh-hSpCas975 (Addgene 42230), a gift from Feng Zhang ...
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bioRxiv - Cancer Biology 2022Quote: The pX330-U6-Chimeric_BB-CBh-hSpCas9 (Addgene#42230), LentiCRISPR v2 expression vector (Addgene# 52961) ...
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bioRxiv - Cell Biology 2023Quote: pX330-U6-Chimeric_BB-CBh-hSpCas9 (no. 42230, Addgene) plasmids containing guide RNAs designed with CRISPOR (http://crispor.tefor.net) ...
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bioRxiv - Cancer Biology 2024Quote: ... and U6 promoter from pX333 vector (Addgene, #64073). Venus was replaced with mTagBFP2 (from lentiGuide-Hygro-mTagBFP2 ...
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bioRxiv - Cancer Biology 2020Quote: ... PCR amplified products were cloned into BsmB1-digested LRG2.1 (a lentiviral sgRNA expression vector, U6-sgRNA-EFS-GFP, Addgene: 108098) using the Gibson Assembly kit (NEB#E2611) ...
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bioRxiv - Bioengineering 2022Quote: ... The hU6 promoter fragment was generated by amplifying the U6 promoter with primer set AA-MluI-U6-gib-FW/AA-U6-sg1-scaf-short-RV from the plasmid backbone pSI-359 (Addgene 131131) and the dsRed filler fragment was generated by amplifying a portion of the dsRed gene from CMVp-dsRed2-Triplex-HHRibo-gRNA1-HDVRibo-pA (Addgene 55201 ...
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bioRxiv - Neuroscience 2024Quote: The pAAV-CMV:Cas9-T2A-mCherry/U6:sgRNA plasmid was modified from pX601 (pAAV-CMV:NLS-SaCas9-NLS-3xHA-bGHpA;U6::BsaI-sgRNA; Addgene plasmid # 61591). To visualize the AAV-infected cells ...
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bioRxiv - Biophysics 2019Quote: ... and mCherry-Paxillin-22 (Addgene plasmid # 55114 ...
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bioRxiv - Cell Biology 2021Quote: ... mApple-Paxillin-22 (Addgene, #54935), mN2-TS (Woroniuk et al ...
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bioRxiv - Cell Biology 2019Quote: ... BbsI digested fragment containing gRNA core and dU6:3 promoter was PCR amplified from pCFD4-U6:1_U6:3-tandemgRNAs (gift from Simon Bullock (Addgene plasmid # 49411) (Port et al. ...
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bioRxiv - Synthetic Biology 2022Quote: ... The resulting PCR amplicon was assembled with a level 0 part encoding either an Arabidopsis U6-26 promoter (AtU6-26 Addgene#68261) or a N ...