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Citations for Addgene :
1 - 50 of 862 citations for Zinc Finger With KRAB And SCAN Domains 5 ZKSCAN5 Antibody HRP since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... the KRAB(KOX1) domain from dCas9-BFP-KRAB (Addgene # 46911) was fused to the C-terminus of dCas9 ...
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bioRxiv - Microbiology 2024Quote: The KRAB domain from pHR-SFFV-dCas9-BFP-KRAB (Addgene plasmid #46911) was cloned into plasmid pHR-SFFV-ZF3-P2A-BFP using Gibson assembly (New England Biolabs Cat#E2611S ...
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bioRxiv - Cancer Biology 2019Quote: ... the KRAB domain was PCR amplified using pHR-SFFV-dCas9-BFP-KRAB (Addgene #46911) as a template ...
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bioRxiv - Bioengineering 2020Quote: ... KRAB domain was derived from pHAGE EF1α dCas9-KRAB (Addgene 50919, Kearns et al., 2014). VPR complex was derived from lenti-EF1a-dCas9-VPR-Puro (Addgene 99373 ...
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bioRxiv - Genomics 2022Quote: ... dCas9-KRAB domain (pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro was a gift from Charles Gersbach (Addgene plasmid # 71236 ...
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bioRxiv - Genomics 2023Quote: ... the DNA sequence of KRAB repressor domain was amplified by PCR from the pHAGE TRE dCas9-KRAB (Addgene plasmid #50917) and cloned in frame into the PB-TRE-dCas9-VPR backbone (Addgene plasmid #63800 ...
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bioRxiv - Neuroscience 2021Quote: ... The amplified fragments were inserted using KpnI and MluI sites into the pCAG vector backbone containing a zinc-finger binding site upstream of a CAG promoter prepared from pCAG-GPHN.FingR-EGFP-CCR5TC (Addgene #46296)17.
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bioRxiv - Synthetic Biology 2020Quote: The TagRFP reporter (5xTetO-pEF-TagRFP-3xNLS) construct was assembled using a AAV zinc finger donor vector backbone (Addgene #22212) containing a promoter-less splice-acceptor (SA ...
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bioRxiv - Molecular Biology 2021Quote: We in-house generated a lentiviral construct expressing dCas9-KRAB-MeCP2 by PCR amplification of the dCas9-KRAB-MeCP2 (contains a domain of MeCP2) from pB-CAGGS-dCas9-KRAB-MeCP2 (Addgene 110824), and then insert it to the pLenti-EF1a-dCas9-VP64-2A-Blast backbone (Addgene 61425 ...
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bioRxiv - Genetics 2021Quote: ... and fused in frame with the human ZNF10 KRAB domain (amplified from the pAAVS1-NDi-CRISPRi (Addgene #73498)) or the catalytic domain (CD ...
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bioRxiv - Genetics 2022Quote: ... We made the dCas9-TurboID construct by replacing the KRBA domain on Lenti-dCas9-KRAB-blast (Addgene, 89567) with TurboID sequence (Addgene ...
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bioRxiv - Cancer Biology 2024Quote: ... We amplified 3xFlag-KRAB (KRAB-dCas9 plasmid; Addgene #112195), 3xFlag-VP64 (pSL690 plasmid ...
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bioRxiv - Genomics 2022Quote: ... KRAB(ZIM3) was PCR-amplified from pLX303-ZIM3-KRAB-dCas9 (Addgene # 154472). The final construct is KRAB(ZIM3)-dCas9 or Zim3-dCas9 for short ...
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bioRxiv - Cancer Biology 2023Quote: ... KRAB-dCas9 (#96918; Addgene) was transduced by lentivirus and successfully transduced cells were selected by 6 μg/ml blasticidin (R21001 ...
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bioRxiv - Genomics 2023Quote: ... lentiviral particles encoding dCas9-BFP-KRAB were generated by transfecting HEK293T cells with plasmids encoding dCas9-BFP-KRAB (pHR-SFFV-dCas9-BFP-KRAB, Addgene 46911) and the ViraPower lentiviral packaging mix (ThermoScientific) ...
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bioRxiv - Genomics 2022Quote: ... dSpCas9-BFP-KRAB was obtained from pHR-SFFV-dCas9-BFP-KRAB (Addgene 46911). Constructs were cloned using Gibson Assembly (NEBuilder HIFI DNA Assembly ...
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bioRxiv - Genomics 2023Quote: ... A dCas9-KRAB-MeCP2-T2A insert was amplified from dCas9-KRAB-MeCP2 (Addgene #110821). A T2A-mCherry Gblock was synthesized by IDT ...
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bioRxiv - Bioengineering 2023Quote: ... or KRAB CRISPRi downregulation expression vector pLV-hUbC-dCas9-KRAB-T2A-GFP (Addgene, #67620). Successful gRNA insertion was verified through Sanger sequencing12,29,38.
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bioRxiv - Cell Biology 2020Quote: ... pC13N-dCas9-BFP-KRAB (127968, Addgene) was integrated in the U2OS genome using F-Talen and R-Talen (pZT-C13-R1 and pZT-C13-L1 ...
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bioRxiv - Genomics 2022Quote: ... with pCMV-sadCas9-KRAB (Addgene, 115790) or pAAV-U6-sasgRNA-CMV-mCherry-WPREpA (92 ...
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bioRxiv - Genomics 2021Quote: SFFV-dCas9-BFP-KRAB (Addgene 46911), CRISPRi/a v2 (Addgene 84832) ...
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bioRxiv - Genomics 2023Quote: ... and dCas9-KRAB-blast (Addgene, #89567) plasmids were separately used to perform CRISPRi and CRISPRa experiments ...
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bioRxiv - Cell Biology 2022Quote: A lentiviral plasmid encoding the KRAB-dCas9-EGFP (pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-GFP) (Addgene plasmid # 71237 ...
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bioRxiv - Genetics 2019Quote: ... To generate stable 786-O-dCas9-KRAB expressing cell line the Lenti-dCas9-KRAB-blast (Addgene #89567) was used ...
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bioRxiv - Genomics 2023Quote: ... K562 CRISPRi and RPE1 CRISPRi cells expressing dCas9-BFP-KRAB (pHR-SFFV-dCas9-BFP-KRAB; Addgene, 46911) were described previously.136 Lenti-X 293T cells were purchased from Takara (#632180) ...
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bioRxiv - Cancer Biology 2020Quote: ... LNCaP cells stably expressing dCas9-KRAB were generated by transducing LNCaP cells with Lenti-dCas9-KRAB (Addgene #89567) followed by blasticidin selection ...
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bioRxiv - Cancer Biology 2023Quote: ... with the KRAB-MECP2 sequence from the transient expression vector of dCas9-KRAB-MECP2 (for CRISPRi, Addgene #110821)100 ...
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bioRxiv - Systems Biology 2022Quote: ... we prepared the vector backbone by incubating 5 ug of UCOE-SFFV-dCas9-BFP-KRAB (Addgene #85969) for 2 h at 37 °C with 2 ul of FastDigest BamHI (ThermoFisher cat ...
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bioRxiv - Neuroscience 2021Quote: ... into pLX_311-KRAB-dCas9 (Addgene cat. #96918) using the NheI and MluI restriction enzymes to generate pLX-eGFP-hCldn5 ...
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bioRxiv - Genomics 2021Quote: The lentiviral dCas9-KRAB plasmid (Addgene #83890) was generated by cloning in a P2A-HygroR (APH ...
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bioRxiv - Cell Biology 2021Quote: ... pHR-SFFV-dCas9-BFP-KRAB (Addgene #46911),20 a gift from Stanley Qi and Jonathan Weissman was stably expressed in HEK293T cells ...
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bioRxiv - Genomics 2019Quote: ... 500ng of dCas9-KRAB-MeCP2 (Addgene: 110821) or dCAS9 (Addgene ...
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bioRxiv - Cell Biology 2022Quote: ... pB-CAGGS-dCas9-KRAB-MeCP2 (Addgene, 110824) and pPB-dCas9-KRAB (kindly provided by Dr ...
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bioRxiv - Cancer Biology 2023Quote: ... dCas9-KRAB-blasticidin (CRISPR interference, Addgene #89567), or dCas9-VPR-blasticidin (CRISPR activation ...
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bioRxiv - Developmental Biology 2024Quote: ... the dCas9-KRAB-meCP2 repressor (Addgene 110821)55 was used ...
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bioRxiv - Cancer Biology 2024Quote: ... and 3xFlag (KRAB-dCas9 plasmid; Addgene #112195) fragments using Herculase II Fusion DNA Polymerase (Agilent Technologies ...
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bioRxiv - Developmental Biology 2019Quote: nls::dCas9-KRAB::nls was derived from “pLV-dCas9-KRAB-PGK-HygR” (Klann et al., 2017) (Addgene plasmid: #83890) and inserted downstream of the promoter Mesp (Davidson ...
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bioRxiv - Genetics 2022Quote: ... using the pUAST-attB from the Drosophila Genomics Resource Center (# 1419) and dCas9-KRAB (Addgene SID4X-dCas9-KRAB; #106399). The primers are listed in the S8 Table and the PCR amplifications were performed using the CloneAmp™ HiFi PCR Premix (Takara) ...
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bioRxiv - Microbiology 2020Quote: ... incorporating the inducible dCas9-KRAB-MeCP2 fusion cassette from PB-TRE-dCas9-KRAB-MeCP2 (gift from Andrea Califano, Addgene # 122267), the EF1a promoter driven rtTA fragment from PB-CA-rtTA Adv (gift from Andras Nagy ...
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bioRxiv - Cell Biology 2019Quote: ... The cells were transduced first by lentivirus expressing dCas9 fused to the KRAB repressor module9 (pHR-SFFV-dCas9-BFP-KRAB, Addgene). Lentivirus-expressing cells were sorted (FACSAria II ...
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bioRxiv - Molecular Biology 2019Quote: ... the KRAB sequence was PCR amplified from the pLV hU6-sgRNA hUbC-dCas9-KRAB-T2A-Puro vector (Addgene, plasmid #71236) and cloned into the enCRISPRa sgRNA vector to replace VP64 ...
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bioRxiv - Molecular Biology 2022Quote: MCF10A-YAP5SA cells expressing Cas9-KRAB were generated by infection with lentiviral vector pHAGE TRE dCas9-KRAB (Addgene #50917; (34)) and selection with neomycin ...
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bioRxiv - Cell Biology 2024Quote: ... or pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro (pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro was a gift from Charles Gersbach (Addgene plasmid # 71236 ...
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bioRxiv - Cancer Biology 2019Quote: pHR-SFFV-KRAB-dCas9-P2A-mCherry (Addgene #60954) was used to generate stable M07e expressing dCas9-KRAB fusion protein cell line ...
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bioRxiv - Microbiology 2022Quote: Plasmid dCAS9-KRAB-MeCP2 (#110821) purchased from Addgene was packaged with lentiviruses and used to transduce LCLs for 2 days followed by selection with 5ug/ml blasticidin for another 5 days ...
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bioRxiv - Cancer Biology 2019Quote: ... A dCas9-KRAB construct (Addgene; Cat. No. 85969) was used to knock down target gene expression ...
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bioRxiv - Molecular Biology 2020Quote: ... pLV-dCas9-KRAB-PGK-HygR (Addgene plasmid #83890) was described previously50 ...
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bioRxiv - Cancer Biology 2020Quote: ... or pHAGE TRE dCas9-KRAB (Addgene plasmid # 50917) using a standard protocol ...
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bioRxiv - Neuroscience 2022Quote: ... and the plasmids pLX_311-KRAB-dCas9 (Addgene #96918), pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro (Addgene #71236) ...
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bioRxiv - Genomics 2023Quote: ... LNCaP cells stably expressing dCas9-KRAB (Addgene #89567) were seeded in a 6-well plate at a density of 200K cells per well ...