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Citations for Addgene :
1 - 45 of 45 citations for Water Nuclease Free Dist since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... Cas9 nuclease (Addgene #1074181), tracrRNA (Addgene #1072532) ...
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bioRxiv - Genetics 2019Quote: ... Nucleases were amplified from Addgene plasmids indicated in Supplemental Table S1 ...
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bioRxiv - Neuroscience 2021Quote: ... 7 μg Cas9 nuclease plasmid (pX459, Addgene #62988) 1.4 μg pPN298 and 1.4 μg pPN306 were electroporated into 2.5×106 cells at 1050V ...
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bioRxiv - Genomics 2021Quote: ... nuclease-deficient dCas9-KRAB-MeCP2 (Addgene plasmid no. 110821) was stably introduced in HEK293T cells using the piggy-transposase system (System Biosciences ...
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A human cancer cell line initiates DNA replication normally in the absence of ORC5 and ORC2 proteinsbioRxiv - Molecular Biology 2020Quote: ... Human codon optimized Cas9 nuclease (hCas9) was used (Addgene (41815)) ...
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bioRxiv - Neuroscience 2021Quote: ... Dox-inducible CRISPR nuclease (CRISPRn) plasmid (pAAVS1-PDi-CRISPRn, Addgene) was used as a starting material ...
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bioRxiv - Cell Biology 2021Quote: ... the equivalent nuclease-deficient Cas9 was obtained from Addgene (#72269). All plasmids were verified by DNA sequencing.
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bioRxiv - Genetics 2023Quote: The CMV-driven SaCas9 nuclease vector pX60143 (Addgene plasmid #61591) was a gift from Feng Zhang (Massachusetts Institute of Technology) ...
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bioRxiv - Neuroscience 2022Quote: ... and humanised Cas9 nuclease vector (a gift from George Church, Addgene plasmid # 41815 ...
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bioRxiv - Genomics 2019Quote: ... the gRNA expression plasmid AX03_pgEGFP and the hCas9 nuclease plasmid (Addgene plasmid 41815) were used for generating DSBs at the EGFP sequence ...
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bioRxiv - Physiology 2021Quote: Production of Cas9 nuclease mRNA utilized the pMLM313 expression vector (Addgene plasmid #42251), which harbors the T7 promoter site upstream of the translational start site ...
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bioRxiv - Cell Biology 2022Quote: ... parental HT1080 cells expressing stable nuclease-dead Cas9 (dCas9-BFP-KRAB, Addgene # 46911) were used as described previously23 ...
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bioRxiv - Synthetic Biology 2021Quote: The nuclease-deficient Cas9 was obtained from pHR-SFFVdCas9-BFP-KRAB (dCas9; plasmid 46911, Addgene). The gRNA containing MS2 aptamer sequences (gRNA_2 ms2 ...
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bioRxiv - Genetics 2023Quote: ... selected SaCas9 sgRNAs were cloned into the TBG-driven SaCas9 nuclease rAAV vector pX60243 (Addgene plasmid #61593 ...
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bioRxiv - Cancer Biology 2024Quote: ... Efficient nuclease activity was confirmed using a reporter system (Addgene #67979, #67980; Supp. Fig. 1). We synthesized a custom single guide RNA (sgRNA ...
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bioRxiv - Molecular Biology 2022Quote: ... yeast expression constructs for nuclease-inactivated Cas9 (D10A/H840A mutations) from Streptococcus pyogenes –dCas9 (Addgene #46920) (Gilbert et al 2013 ...
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bioRxiv - Developmental Biology 2021Quote: ... AAAC-CCTGAGCAGAAGAGTGGTTA-C) were designed and ligated into the RNA-guided nuclease plasmid (pX330-mCherry plasmid; Addgene), in order to induce the Cas9-mediated DSB in the genomic DNA of the Dystrophin-deficient iPSCs ...
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bioRxiv - Molecular Biology 2021Quote: ... 700ng/mL of proteinA-micrococcal nuclease (pA-Mnase purified in house with vector from Addgene 86973 79) were incubated with the nuclei at 4 degrees for an hour ...
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bioRxiv - Cell Biology 2022Quote: ... The sgRNA oligos (Table S2) were introduced into pX458 expressing Cas9 nuclease fused to GFP (Addgene #48138). pSpCas9(BB)-2A-GFP (PX458 ...
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bioRxiv - Biophysics 2023Quote: ... Transfections contained 70 ng of SpCas9 nuclease plasmid (pCMV-T7-SpCas9-P2A-EGFP (RTW3027; Addgene ID 139987) and 15 ng each of two sgRNA expression plasmids mixed with 0.3 µL of TransIT-X2 (Mirus ...
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bioRxiv - Molecular Biology 2020Quote: ... AsCas12a and SpCas9 nucleases were expressed from the pET-based T7 promoter-containing plasmids (Addgene, Plasmids #90095, #62374) in Escherichia coli strain BL21-DE3 as described (3 ...
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bioRxiv - Neuroscience 2022Quote: ... and humanised Cas9 nuclease vector (a gift from George Church, Addgene plasmid # 41815 ; http://n2t.net/addgene:41815 ; RRID:Addgene_41815) (Mali et al. ...
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bioRxiv - Genetics 2023Quote: ... following the manufacturer’s protocol with AAVS1 targeting vector and predesigned transcription activator-like effector nucleases (hAAVS1 TALEN Left and Right were gifts from Su-Chun Zhang, Addgene plasmid # 52341 & 52342 ...
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bioRxiv - Biochemistry 2024Quote: ... expressing Cas9 nuclease and two gRNAs (see below for sequences) together with the CRIS-PITCh vector pX330S-2-PITCh (63670, Addgene), harboring the Lamin A microhomologies and GFP-Puro or GFP-Neo/Kan insertions ...
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bioRxiv - Molecular Biology 2019Quote: The gene encoding full length Francisella novicida (Fn) Cas9 nuclease residues 1-1629 bp) was PCR amplified using PX408 (Addgene 68705) as a template and cloned in pET28-His-10-Smt3 vector (a kind gift from Prof ...
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bioRxiv - Genetics 2020Quote: Human codon-optimized high fidelity Cas9 nuclease construct with GFP tag (pSpCas9(BB)-2A-GFP (PX458))80 was obtained from Addgene (#48138). Two sgRNAs were designed using CRISPR-Cas9 guide RNA design checker from Integrated DNA Technologies ...
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bioRxiv - Cancer Biology 2019Quote: The B-cell acute lymphoblastic leukemia cell lines NALM-6 and REH were lentivirally transduced with plasmid expressing Cas9 nuclease (LentiCRISPR V2; Addgene Plasmid #52961) as described below at a MOI of 0.5 ...
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bioRxiv - Developmental Biology 2021Quote: The p133-pPB plasmids encoding for the Tal1-gRNAs were co-transfected with the pX458 plasmid containing a Cas9 nuclease construct and a GFP reporter (pSpCas9-2A-GFP, Addgene ID: 48138), kindly provided by Jamie Hackett from EMBL Rome ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell lines stably expressing the Cas9 nuclease were generated by infection with the empty lentiCRISPRv2 lentivirus (Addgene, #52961, not containing an sgRNA guide) using standard methods (https://www.addgene.org/viral-vectors/lentivirus/lenti-guide/) ...
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bioRxiv - Cell Biology 2023Quote: ... vectors encoding a pair of AAVS1-specific zinc finger nucleases (AAVS1-ZFN-L and AAVS1-ZFN-R) and an AAVS1 Neo-CAG-rtTA donor template were obtained from Addgene (plasmids #60915, #60916, and #60431). For transfection ...
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bioRxiv - Molecular Biology 2020Quote: ... supplemented with 2 ng/μl RNase-free plasmid DNA (pX330, Addgene #42230) in a 15 ml Falcon tube ...
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bioRxiv - Molecular Biology 2020Quote: 2 μl RNase-free plasmid DNA (pX330, Addgene #42230, 100 ng/μl),
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bioRxiv - Synthetic Biology 2020Quote: ... The ‘Cell-free to Clostridium’ vectors were derived from pJL1 plasmid (Addgene #69496), modified in the T7 promoter region to contain a BsaI recognition site between the RBS and START codon in three variations to generate pD1 ...
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bioRxiv - Neuroscience 2021Quote: ... (a gift from Loren Looger, Addgene plasmid # 98929; http://n2t.net/addgene:98929; RRID:Addgene_98929; 1013 vg/mL in water) was injected into the vitreous humour of wild type mice (C57BL/6J ...
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bioRxiv - Neuroscience 2023Quote: ... Embryos were injected with plasmid mixture (1.5 μg/uL pCAG-ChR2-mRuby-ST in 0.5% Fast Green in UltraPure water, Addgene 109125) in the left ventricle using a glass micropipette pulled to a 70 μm beveled tip ...
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bioRxiv - Neuroscience 2023Quote: ... 1 µL of AAV9.hsyn.FLEX.iGluSnFR.WPRE.SV40 (a gift from Loren Looger, Addgene plasmid # 98931; http://n2t.net/addgene: 98931; RRID:Addgene_98931, 10 vg/mL in water) solution was injected with a blunt tip (30 gauge ...
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bioRxiv - Neuroscience 2022Quote: ... One microliter of endo-free purified (2 ug/ul) pCAG-EGFP plasmid (Addgene, cat# 89684) mixed with 0.05% Fast Green (Sigma ...
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bioRxiv - Molecular Biology 2024Quote: ... Annealing reactions were diluted 1:10 with water and then 1µL was used to ligate into 100ng of BsmBI digested pLentiGuidePuro vector (Addgene #52963) in 1x T4 DNA Ligase Buffer ...
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bioRxiv - Genetics 2021Quote: ... for the deletion step using a one-step cloning reaction as described by the Thomas lab 25 The same protocol with the minor modification of replacing the second oligo with water during ligation was used to clone the syn-sgRNAs into pX459 (Addgene #62988). A cloning mixture volume of 5μL or 10μL was then transformed into 25μL or 50μL ...
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bioRxiv - Microbiology 2023Quote: ... The annealed oligos were diluted 250-fold with water and used for ligation with the pSpCas9(BB)-2A-Puro (PX459) V2.0 vector (Addgene, Cat# 62988), which was predigested with BbsI-HF (NEB ...
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bioRxiv - Cell Biology 2023Quote: ... we measured intramitochondrial free Ca2+ levels by transfecting AML12 cells with the CMV-mito-GEM-GECO1 plasmid (Addgene, 32461 ...
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bioRxiv - Cancer Biology 2023Quote: ... Transfer lentiviral plasmids (2500 ng) were diluted in 200 µl serum-free medium together with 225 ng pCMV-VSV-G (Addgene #8454) and 2250 ng psPAX2 (Addgene #12260 ...
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bioRxiv - Synthetic Biology 2023Quote: ... the CMV promoter of the dsRed2-free plasmid was replaced with the EF1α promoter amplified from pCEP4_WT_OCT4 (Addgene cat no. 40629) by Gibson assembly after KpnI and SacI restriction ...
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bioRxiv - Neuroscience 2022Quote: ... ACS-4500) using Lipofectamine 2000 in FBS free Optimem together with the virus packaging plasmids (psPAX2 and pMD2-G, Addgene # 12260 and # 12259) and the plasmid expressing either wild-type U4atac or an Empty Vector ...
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bioRxiv - Cell Biology 2021Quote: ... The co-expression plasmid of free Clover and mRuby2 was constructed by inserting an internal ribosome entry site (cloned from Addgene #127332; a gift from Jeremy Wilusz) between the Clover and mRuby2 of pcDNA3.1-Clover-mRuby2 at the AgeI site ...