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Citations for Addgene :
1 - 50 of 342 citations for Vertical Gel Casting Cassettes since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... the biRhoBASTn cassette was gel purified and ligated into XhoI-digested and dephosphorylated mAzurite-C1 (Addgene Plasmid #54583) to yield mAzurite-biRhoBASTn ...
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bioRxiv - Microbiology 2023Quote: ... and gRNA cassette (pSPgRNA: Addgene #47108) plasmids had sequences that target genomic CDK8 ...
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Self-organization of embryonic stem cells into a reproducible embryo model through epigenome editingbioRxiv - Developmental Biology 2024Quote: The pSLQ1371-sgRNA cassette (Addgene, 121425) was integrated via lentiviral transduction ...
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bioRxiv - Molecular Biology 2022Quote: ... U6-pegRNA cassette expressing the ATP1A1-Q118R and T804N cassettes were cloned upstream of the U6-RFP-acceptor cassette to create ATP1A1_G4_Q118R_Dual_pegRNA (Addgene 173199) and ATP1A1_G6_T804N _Dual_pegRNA (Addgene 173200) ...
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bioRxiv - Cell Biology 2022Quote: ... and the U6-sgRNA cassette was replaced by a rTet cassette under an UBC promoter (taken from Addgene plasmid # 50917 ...
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bioRxiv - Molecular Biology 2022Quote: ... The U6-ATP1A1 gRNA (G2) cassette was also cloned upstream of the U6-BbsI-sgRNA cassette to create ATP1A1_G2_Dual_sgRNA (Addgene 173201) for ABE coselections ...
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bioRxiv - Molecular Biology 2022Quote: ... The U6-ATP1A1 nick gRNA cassettes were cloned upstream of the U6-BbsI-sgRNA cassette to create ATP1A1_G3_Dual_sgRNA (Addgene 173202) and ATP1A1_G8 _Dual_sgRNA (Addgene 178104) ...
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bioRxiv - Synthetic Biology 2020Quote: ... This reporter cassette was assembled with a plant selectable marker cassette conferring resistance to kanamycin (pEPYC1CB0308, Addgene T.B.C.). Synthetic and control promoters were additionally fused to either a transcription activator like effector (TALE ...
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bioRxiv - Developmental Biology 2020Quote: ... the Gateway cassette from FuTetO-GW (Addgene) was cloned by Gibson assembly into the Asc1/Pme1 site of Hprt2 (Serafimidis et al. ...
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bioRxiv - Physiology 2021Quote: ... in a Flex(FRT) cassette (using Addgene plasmid #55641 ...
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bioRxiv - Microbiology 2021Quote: ... chloramphenicol resistance cassette (amplified from pPEP1, Addgene plasmid #61046 [81] ...
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bioRxiv - Synthetic Biology 2020Quote: ... the LEU2 cassette of pRSII42528 (Addgene #35468) was replaced by a Sh ble cassette conferring zeocin resistance ...
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bioRxiv - Neuroscience 2023Quote: ... the cassette pCMV-Apobec1-Yth (Addgene #131636) and pCMV-Apobec1-Ythmut (Addgene #131637 ...
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bioRxiv - Genomics 2023Quote: ... We replaced the hU6-sgRNA cassette with the mU6-sgRNA cassette from pMK1334 (ref. 1) (Addgene plasmid #127965, RRID:Addgene_127965) by restriction cloning between sites MluI and XbaI ...
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bioRxiv - Neuroscience 2021Quote: Recombinant adeno-associated virus (AAV) vector containing a CCL2 expression cassette w as generated by replacing GFP cassette of pAAV-CAG-GFP (Addgene) with the full-length c DNA for CCL2 (OriGene) ...
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bioRxiv - Genomics 2023Quote: ... RRID:Addgene_85451)20. We replaced the hU6-sgRNA cassette with the mU6-sgRNA cassette from pMK1334 (ref. 1) (Addgene plasmid #127965, RRID:Addgene_127965) by restriction cloning between sites MluI and XbaI ...
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bioRxiv - Genomics 2023Quote: ... We further replaced the Cas9 cassette with an nCas9/M-MLV-RT cassette from the pCMV-PE2 plasmid (Addgene, 132775). The lentiV2-pegRNA and lentiV2-ngRNA plasmids were constructed by replacing the Cas9 and Puromycin sequences in the lentiCRISPR v2 plasmid (Addgene ...
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bioRxiv - Physiology 2020Quote: ... The expression cassettes of dCas9-VP64 (Addgene: 61422) and MS2-P65-HSF1 (Addgene ...
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bioRxiv - Plant Biology 2021Quote: ... created by inserting the Stubi3P:nptII:Stubi3T cassette from AddGene plasmid 59175 between the XbaI/KpnI sites of pGGP-A-G [57].
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bioRxiv - Genetics 2022Quote: ... We PCR amplified the NatMX cassette from Addgene plasmid #35121 using primers with homology to the 5’ upstream and 3’ downstream sequences of the targeted gene ...
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bioRxiv - Neuroscience 2023Quote: ... A doxycycline-inducible neurogenin2 (NGN2) cassette (Addgene #105840) was stably integrated into each iPSC line ...
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bioRxiv - Neuroscience 2023Quote: ... the DIO-EGFP cassette was amplified from Addgene Plasmid #50457 and subcloned into SalI and EcoRI sites ...
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bioRxiv - Microbiology 2023Quote: ... difficile chloramphenicol resistance cassette (catP in pRPF185; Addgene 106367 49 and the Saccharopolyspora erythraea erythromycin resistance cassette (ermE) ...
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bioRxiv - Genetics 2023Quote: ... the PEmax cassette from pCMV-PEmax (Addgene 174820) was swapped with the indicated cassette.
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bioRxiv - Cell Biology 2022Quote: ... pLVX-Puro APEX2-GFP-TMEM11 was generated by PCR amplifying the GFP-TMEM11 cassette and the APEX2 cassette from pcDNA3 Connexin43-GFP-APEX2 (kindly provided by Alice Ting; Addgene #49385) (Lam et al. ...
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bioRxiv - Plant Biology 2023Quote: ... The final construct was assembled by combining the two Level 1 sgRNA cassettes with cassettes for resistance to kanamycin pICSL11024 (Addgene#51144) and constitutive expression of SpCas9 (pEPQD1CB0001 ...
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bioRxiv - Molecular Biology 2023Quote: A pU6-Sp-dual-gRNA scaffold vector was generated by replacing the pegRNA expressing cassette of pU6-Sp-pegRNA-HEK3-insCTT vector with a dual U6-gRNA cassette from the PX333 vector (Addgene: 64073)65 ...
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bioRxiv - Cancer Biology 2020Quote: ... MYC and AR over-expression a derivate of the pLX304 vector was used throughout in which the CMV promoter has been exchanged to a PGK promoter and the blasticidin cassette left unchanged (ΔERG constructs) or exchanged by a puromycin resistance cassette (SPOP constructs) (pLX_TRC_307, available at Addgene as Plasmid 41392, pCW107). All ORFs were cloned into pLX_TRC_307 using Nhe1 and Mlu1 ...
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bioRxiv - Cell Biology 2020Quote: ... the combinatorial gRNA-expressing cassette of pKLV2.2 (Addgene: 72666) was digested with AleI and BsiWI ...
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bioRxiv - Genetics 2022Quote: The SpCas9-EGFP-bGH poly(A) cassette from Addgene plasmid #61408 was cloned into the pStart-K vector (Addgene #20346) ...
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bioRxiv - Bioengineering 2021Quote: ... (2) chloramphenicol resistance cassette from pTARA[52] (Addgene #39491), (3 ...
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bioRxiv - Bioengineering 2021Quote: ... (2) chloramphenicol resistance cassette from pTARA[52] (Addgene #39491), (3 ...
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bioRxiv - Genomics 2019Quote: ... amplifying a bacterial GFP expression cassette from pWS158 (Addgene), and assembled using gibson isothermal assembly to generate DGP306.
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bioRxiv - Bioengineering 2020Quote: ... and Cam targeting cassette (CTV) (Addgene, cat. no. 131590) are available from Addgene ...
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bioRxiv - Microbiology 2019Quote: ... The sgRNA cassette from pgRNA-bacteria 9 (Addgene plasmid # 44251) was introduced into pSCrhaB2 by restriction cloning with EcoRI and HindIII (NEB ...
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bioRxiv - Neuroscience 2020Quote: ... with the tau expression cassette sub-cloned into pCFJ151 (Addgene) to generate MosSCI inserts at the ttTi5605 loci in C ...
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bioRxiv - Bioengineering 2022Quote: ... as well as the LacZ cassette from pUC19 (Addgene #50005) with a T7 promoter sequence followed by a BsaI restriction site for subsequent cloning steps ...
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bioRxiv - Microbiology 2021Quote: ... Sulf or Bar gene cassette in the pFGL821 (Addgene, 58223), pFGL820 (Addgene ...
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bioRxiv - Cancer Biology 2020Quote: ... lentiCRISPRv2 (containing hSpCas9 and the chimeric guide RNA cassettes; Addgene), was digested using BsmBI ...
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bioRxiv - Immunology 2021Quote: ... replacing the Puromycin selection cassette in LentiGuide-Puro (Addgene #52963) with the sequence encoding mCherry ...
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bioRxiv - Genetics 2020Quote: ... and a blue fluorescent protein (BFP) expression cassette (Addgene #36086). The 1kb upstream and 1kb downstream arms were amplified from purified mouse genome from AB2.2 ES cells (ATCC #SCRC-1023 ...
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bioRxiv - Neuroscience 2022Quote: ... One insert consisted of the U6-sgRNA cassette from Addgene 71236 (gifted from Charles Gersbach [46]) ...
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bioRxiv - Genomics 2023Quote: ... The Cas9-guide cassette was derived from plasmid pML104 (Addgene) (Figure S1) ...
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bioRxiv - Genetics 2023Quote: ... the full resistance cassette was amplified from pCFJ910 (Addgene ID44481) and assembled into PCR linearized pUC19 vector to give pZCS38.
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bioRxiv - Genetics 2024Quote: ... containing BrdU cassette obtained from Addgene (Addgene plasmid # 71789-71792), were integrated at four different auxotrophic loci (HIS3 ...
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bioRxiv - Cell Biology 2021Quote: ... and GFP-LC3-RFP-LC3ΔG (reporter cassette from Addgene plasmid #84572)10 reporters were transduced into iAstrocytes with lentivirus at low multiplicity of infection (MOI ...
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bioRxiv - Immunology 2020Quote: ... ubiquitination-based cell cycle indicator (Fucci) 55 cassette (pEN435, Addgene #92139) was stably introduced into the Rad21-degron and CTCF-degron v-Abl pro-B lines following the strategy as described previously9 ...
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bioRxiv - Microbiology 2020Quote: ... A counter-selection cassette derived from pGOAL17 (Addgene plasmid #20189; (46)) was then inserted into p4185K to generate p4185K17 ...
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bioRxiv - Microbiology 2019Quote: ... or a chloramphenicol resistance cassette from plasmid pKD3 (Addgene plasmid #45604) (53 ...
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bioRxiv - Developmental Biology 2021Quote: ... a multiple cloning cassette in pGGEV_6 and pGGEV_7’ _linker (#49293 Addgene) into ...