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Citations for Addgene :
1 - 50 of 145 citations for Vertical Electrophoresis system since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Fragments were purified by agarose gel electrophoresis and individually subcloned into BamHI/NotI-digested pBluescript II KS+ (Addgene). Colonies positive for the plasmid were selected on LB agar with 100 μg/ml ampicillin (Gibco from Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... or LentiGuide-Puro system (two-vector system, Addgene #52963 and #52962), which were gifts from Feng Zhang ...
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bioRxiv - Molecular Biology 2022Quote: ... We used Venus system (Addgene) by fusing VN210 (Venus aa1-210 ...
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bioRxiv - Synthetic Biology 2022Quote: ... GreenGate Cloning System (Addgene Kit #1000000036) and amilCP_Orange chromoprotein vector (Addgene Plasmid #117850 ...
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bioRxiv - Cell Biology 2023Quote: ... the CRISPR/Cas9 system (pX330, Addgene 42230 ...
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bioRxiv - Cell Biology 2021Quote: The generation of stable knockout cell lines was achieved using the LentiCRISPRv2 system (one-vector system, Addgene #52961) or LentiGuide-Puro system (two-vector system ...
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bioRxiv - Cell Biology 2021Quote: The degradFP system (Markus Affolter26, Addgene- 35579) was amplified by PCR and inserted into PJFRC19 containing the p-element promoter and nosTCE-pgc 3’UTR as described above ...
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bioRxiv - Cancer Biology 2021Quote: ... a retrovirus-based expression system (MigR1, Addgene #27490) was used ...
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bioRxiv - Cancer Biology 2021Quote: Second generation lentivirus packaging system (psPAX2 (Addgene #12260), pMD2.G (Addgene #12259 ...
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bioRxiv - Bioengineering 2023Quote: ... Coli Type I Cascade system (Addgene #106270-106275) and Pae Type I Cascade System (Addgene #153942 and 153943) ...
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bioRxiv - Cell Biology 2023Quote: ... Lentiviral packaging system: pRSV-Rev (Addgene plasmid #12253), pMDLg/pRRE (Addgene plasmid #12251) ...
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bioRxiv - Cancer Biology 2023Quote: ... we used a one-vector CRISPRi system (Addgene #71236) to create polyclonal knockdown cell lines ...
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bioRxiv - Cell Biology 2022Quote: ... we used the 2-vector system (lenti-guide Puro; Addgene #1000000049 ...
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bioRxiv - Cancer Biology 2021Quote: Second generation lentivirus packaging system consisting of psPAX2 (Addgene #12260), pMD2.G (Addgene #12259 ...
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bioRxiv - Bioengineering 2023Quote: ... and Pae Type I Cascade System (Addgene #153942 and 153943), YAP-S5A (Addgene #33093 ...
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bioRxiv - Microbiology 2023Quote: The inducible CRISPRi system consisting of pdCaS7-bacteria (AddGene #44249) and pgRNA-bacteria (AddGene #44251 ...
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bioRxiv - Developmental Biology 2021Quote: ... using a three-plasmid system (MSCV-Cre-GFP (Addgene plasmid #24064), MLV Gag-Pol/pHit111 and MLV Env/pHIT60 ...
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bioRxiv - Cell Biology 2021Quote: The human GeCKO v2 library (2 plasmid system) (Addgene plasmid #1000000049) was amplified by electroporation using a Bio-Rad Gene Pulser II electroporation apparatus (Bio-Rad #165-2105 ...
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bioRxiv - Genetics 2020Quote: ... The AAVS1 targeting fluorescent reporter #208 system was modified from Addgene ID 60431 ...
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bioRxiv - Biophysics 2021Quote: ... using the single plasmid system from Feng Zhang’s lab (Addgene #62988). All known splice variants of NMHC IIB and NMHC IIC were targeted by the respective sgRNA ...
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bioRxiv - Molecular Biology 2023Quote: ... the genome editing one vector system (PX459) (104142, Addgene, MA, USA) was used ...
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bioRxiv - Molecular Biology 2023Quote: ... using the pLKO.1 lentiviral vector system (Addgene, Cambridge, MA, USA). A list of the stable cell lines generated is summarized in supplementary table S1.
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bioRxiv - Immunology 2021Quote: ... TXN1 CRISPR sgRNAs were cloned into the pLentiGuide expression system (Addgene #52963) for HEK 293T gene ablation ...
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bioRxiv - Microbiology 2021Quote: The pMD2.G and the psPAX2 packaging system were obtained from Addgene. VSV-G pseudotyped particles were produced by transfection of HEK 293T cells as described previously(33) ...
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bioRxiv - Neuroscience 2021Quote: ... The third generation lentiviral packaging system plasmids pMDLg/pRRE (Addgene, cat. #12251), pRSV-Rev (Addgene ...
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bioRxiv - Cancer Biology 2023Quote: ... we used the gene editing crispr-cas9 system (lentiCRISPR-v2) (Addgene #52961). sgRNAs were designed using the CRISPR tool (http://crispr.mit.edu ...
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bioRxiv - Neuroscience 2024Quote: ... cells were transduced with an inducible system Tet-pLKO-puro (21915, Addgene) encoding an shRNA targeting TARDBP sequence (TRCN0000016038 ...
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bioRxiv - Synthetic Biology 2023Quote: ... Plasmids containing the CAST system were derived from pSL1142 (Addgene plasmid #160730).33 All experiments were performed in wild-type (WT ...
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bioRxiv - Cancer Biology 2022Quote: Gene knockouts were generated by the lentiCRISPRv2 system (Addgene plasmid #83480 and #98291)(Sanjana et al ...
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bioRxiv - Cancer Biology 2022Quote: DNA plasmids were constructed using the pLX302 and pLX304 Gateway system (Addgene, #25890). Briefly ...
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bioRxiv - Immunology 2022Quote: The pRRLSIN-pMD2.G-psPAX2 three plasmids lentivirus system were purchased from Addgene. The FCGRIIIA-F (CD16a ...
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bioRxiv - Molecular Biology 2022Quote: ... CRISPR-Prime Editing system encoding lentiviral pLenti-PE2-BSD plasmid DNAs (Addgene, # 161514) encoding CRISPR-PE containing the blasticidin resistance gene ...
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bioRxiv - Genomics 2023Quote: ... and a “2 vector system” (backbone expresses only the sgRNA library – Addgene #73178). In this study we also used our Essential/Safe-havens library described above.
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bioRxiv - Cell Biology 2023Quote: ... The pcDNA3.1-MYO10-HMM-Nanotrap system was a gift from Thomas Friedman (Addgene plasmid # 87255 ...
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bioRxiv - Systems Biology 2021Quote: Akt KTR was modified from the transposase transfection system PSBbi-FoxO1_1R_10A_3D vector (Addgene # 106278)48 for lentiviral production (Fig ...
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bioRxiv - Cell Biology 2020Quote: Coilin-KO cells were generated using the Cas9 nickase system (pX335 and pKN7; Addgene) [Cong et al ...
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bioRxiv - Biochemistry 2021Quote: ... This utilizes a vector system described previously (Okumoto et al., 2005) (Addgene Plasmid #13537), which contains a pRSET backbone with an N-terminal 6xHis tag and the insertion of a biotin tag from the PinPoint™ Xa-1 Vector (Promega ...
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bioRxiv - Cell Biology 2020Quote: ... The entry vector generated was recombined using the gateway system with pEZYflag (Addgene #18700), pDEST1 or pRK5mycGW (a gift from Fanny Jaulin Lab ...
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bioRxiv - Cancer Biology 2022Quote: ... Luciferase/tdTomatao reporter was engineered using the MuLE system kit from Addgene (Cat. # 1000000060) (54).
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bioRxiv - Molecular Biology 2023Quote: ... CAST I-B system’s proteins and inverted repeat constructs were sub-cloned from Addgene plasmids #168137 and #168146 to generate pIF1003.
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bioRxiv - Synthetic Biology 2024Quote: ... and the sgRNA for the TREE reporter system (164413) were all obtained from Addgene. To generate Ai9 reporter 293T cells ...
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bioRxiv - Cell Biology 2023Quote: ... the CRISPR/Cas9 system (pX330, Addgene 42230; pKN7, Addgene DU70250; and pX459, Addgene 62988) was used ...
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bioRxiv - Cell Biology 2023Quote: ... the CRISPR/Cas9 system (pX330, Addgene 42230; pKN7, Addgene DU70250; and pX459, Addgene 62988) was used ...
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bioRxiv - Molecular Biology 2021Quote: ... Lentivirus was produced in HEK293T cells using a 2nd generation lentiviral packaging system (psPAX2 – Addgene plasmid #12260 and pMD2.G – Addgene #12259 from Didier Trono ...
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bioRxiv - Cancer Biology 2020Quote: ... Lentivirus was produced using HEK293FT cells with the second-generation packaging system pSPAX2 (Addgene plasmid) and pMD2.G (Addgene plasmid) ...
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bioRxiv - Cell Biology 2021Quote: ... All cell lines were generated using the lentivirus Trono group second generation packaging system (Addgene) and selected using puromycin resistance (1 µg/ml ...
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bioRxiv - Microbiology 2019Quote: ... The reprogramming protocol was performed with episomal vectors system (Addgene plasmids 27077, 27078 and 27080), and using the Amaxa human CD34+ cells Nucleofection Kit (Lonza) ...
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bioRxiv - Synthetic Biology 2020Quote: ... The luciferase-based GO system with sgRNA in the same vector was obtained from Addgene (pLenti-mU6-Luc2GO-PGK-Neo ...
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bioRxiv - Cell Biology 2023Quote: ... All cell lines were generated using the lentivirus Trono group second generation packaging system (Addgene) and selected using puromycin resistance (2 µg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... using the 2nd generation packaging system (plasmids psPAX2, Addgene #12260 and pMD2.G, Addgene #12259) were applied to cells for 72h with polybrene (10 µg/ml ...