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Citations for Addgene :
1 - 50 of 466 citations for Two Hybrid System Construction kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... or LentiGuide-Puro system (two-vector system, Addgene #52963 and #52962), which were gifts from Feng Zhang ...
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bioRxiv - Neuroscience 2020Quote: ... Both viral constructions were obtained from Addgene. CNO efficacy in orexin neurons was confirmed in acute slices obtained from injected mice ...
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bioRxiv - Neuroscience 2021Quote: ... Both viral constructions were obtained from Addgene. Clozapine N-Oxide (CNO ...
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bioRxiv - Neuroscience 2023Quote: ... For cloning cytochrome b561-gamillus construction (124837, Addgene), pHluorin was replaced with gamillus ...
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bioRxiv - Plant Biology 2024Quote: ... Plasmid construction was performed by modular cloning using the MoClo Tool Kit and the MoClo Plant Parts Kit (Addgene, 28–30). A detailed MoClo protocol and the list of vectors used can be found in 18.
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bioRxiv - Synthetic Biology 2022Quote: The construction of vectors pCTCON2-BXG (Addgene plasmid #158127),30 pCTCON2-BYG (Addgene plasmid #158144),30 pCHA-Donkey1.1,38 and pCHA-Donkey1.1-H54TAG38 have been described previously ...
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bioRxiv - Cell Biology 2019Quote: The following constructions were used: mCherry-DRP1 (AddGene Plasmid #49152); pDsRed-Mito (AddGene Plasmid #55838) ...
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bioRxiv - Synthetic Biology 2022Quote: ... pDonor plasmid construction: The sgRNA was amplified from pHelper_ShCAST_sgRNA (Addgene #127921), and the transposon was amplified from pDonor_ShCAST_kanR (Addgene #127924) ...
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bioRxiv - Synthetic Biology 2023Quote: ... GFP and mCherry-responsive synNotch construction: pHR_SFFV_myc- LaG17_synNotch_TetRVP64 (Addgene plasmid# 79128) and pHR_EF1a_flag- LaM4_synNotch_Gal4-VP64 ...
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bioRxiv - Genetics 2023Quote: ... the rep/cap hybrid plasmid pAAV2/8 (Addgene #112864 ...
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bioRxiv - Synthetic Biology 2023Quote: ... we performed two-step cloning using a Platinum Gate TALEN kit (Addgene, #1000000043). In the assembly-step 1 ...
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bioRxiv - Cancer Biology 2020Quote: Single knockouts were generated either using a two-vector Streptococcus pyogenes (Sp) Cas9 system: LentiV_SpCas9_puro (Addgene, 108100) and LRG2.1 backbone (Addgene ...
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bioRxiv - Cancer Biology 2020Quote: CRISPR screen was performed in BT20 cells using the GeCKO v2 two vector system (Addgene, CAT# 1000000049). Both A and B libraries were amplified according to the distributor ...
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bioRxiv - Genomics 2023Quote: ... whose construction by APPEAL will be described separately) used in the study originated from Addgene-deposited plasmids including lentiCas9-Blast (#52962) ...
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bioRxiv - Synthetic Biology 2022Quote: ... GreenGate Cloning System (Addgene Kit #1000000036) and amilCP_Orange chromoprotein vector (Addgene Plasmid #117850 ...
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bioRxiv - Cell Biology 2023Quote: Whole-genome CRISPR screens were performed in U2OS and U2OSp53KO cells using the GeCKOv2 two-vector system (Addgene, #1000000049). The two pooled DNA half-libraries (A and B ...
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bioRxiv - Molecular Biology 2023Quote: ... with two px458 (Addgene, 48138) plasmids containing gRNA sequences targeting the deletion breakpoints ...
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bioRxiv - Synthetic Biology 2020Quote: Construction of pGuides and cloning of all spacers utilized pSpgRNA as the base plasmid (Addgene, #47108). All sgRNAs were cloned following the protocol optimized for pX330 base plasmids https://www.addgene.org/crispr/zhang/ ...
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bioRxiv - Neuroscience 2023Quote: ... The two helper AAVs from Addgene were diluted in Dulbecco’s phosphate-buffered saline (DPBS ...
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bioRxiv - Neuroscience 2020Quote: We employed a two-step Golden Gate assembly method using the Platinum Gate TALEN Kit (Addgene; cat#1000000043) to construct Platinum TALEN plasmids containing the homodimer-type FokI nuclease domain ...
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bioRxiv - Biochemistry 2023Quote: ... The SARS-CoV-2 Spike ectodomain Hexa-pro construction (Table 1) was a gift from Jason McLellan (Addgene # 154754 ...
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bioRxiv - Developmental Biology 2022Quote: ... Gene-specific TALEN constructs were assembled using the TALEN Golden Gate assembly system as described [92] with the two backbone plasmids pCS2TAL3DD and pCS2TAL3RR (Addgene, Cambridge, MA). The TALE mRNAs were generated by in vitro transcription using the mMESSAGE mMACHINE SP6 kit (Ambion ...
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bioRxiv - Bioengineering 2022Quote: ... two packaging plasmids (Addgene #8454 and 8455). 50,000 HEK293T were transduced with a MOI of 10 of each lentivirus in a 24-well format and then selected for blasticidin expression (5 μg/ml ...
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bioRxiv - Biochemistry 2019Quote: ... Two previously described plasmids pSADuet259 (Addgene #121910) and pSABAD250 (Addgene #45612 ...
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bioRxiv - Plant Biology 2020Quote: ... two gBlocks and four plasmids from Addgene, pICH47742::2x35S-5′UTR-hCas9 (STOP)-NOST (Addgene no ...
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bioRxiv - Neuroscience 2024Quote: ... two plasmid constructs pmGFP10C-Tau (Addgene, #71433) and pmGFP11C-Tau (Addgene ...
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bioRxiv - Cell Biology 2020Quote: FLOE1’s coding sequence was transferred from G18395 (see Plant vector construction section) into pDEST-HisMBP (Addgene plasmid # 11085) (32 ...
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bioRxiv - Cancer Biology 2021Quote: ... These pLKO.1 constructions were transfected in the 293T packaging cell line along with pMD2.G and pCMV-dR8.91 vectors (Addgene), following a typical Lipofectamine 2000 (Invitrogen ...
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bioRxiv - Immunology 2023Quote: Sleeping Beauty vectors construction: The backbone transposon plasmid pT2/HB was a gift from Perry Hackett (Addgene plasmid # 26557). The transposase plasmid pCMV(CAT)T7-SB100 was a gift from Zsuzsanna Izsvak (Addgene plasmid # 34879) ...
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bioRxiv - Synthetic Biology 2024Quote: ... The construction pUCmini-iCAP-PHP.S for AAV-PHP.S serotype assembly was kindly gifted by Viviana Gradinaru (Addgene plasmid #103006). For targeted expression of constructs in cardiomyocytes ...
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bioRxiv - Genomics 2020Quote: ... Two different plasmids pENO1-iRFP-NATr (Addgene Inc) and pFA-GFP-HIS1 were used to design the repair segment ...
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bioRxiv - Immunology 2021Quote: ... two sgRNA-Cas9 expression vectors (PX458, Addgene # 43138) targeting exon 7 and intron 6 of the CDC42 gene and targeting vector with a floxed puromycin resistance cassette flanked by 2 kb homology arms were introduced into an iPSC clone derived from a healthy control for homologous recombination ...
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bioRxiv - Microbiology 2022Quote: ... in the two lentiviral vector version (Addgene 1000000049), was used (33 ...
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bioRxiv - Microbiology 2023Quote: HMGB1 plasmids for cell line construction were generated in a 2nd generation pLVX-M- puro transfer plasmid (Addgene plasmid# 125839). The HMGB1 sequence was obtained from the pcDNA3.1 Flag hHMGB1 plasmid (Addgene plasmid #31609).
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bioRxiv - Molecular Biology 2021Quote: ... For mutagenesis of the two SIMs in sov two pairs of sgRNAs (1+4 and 3+2) were cloned into pCFD4d (Addgene 83954) (Ge et al. ...
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bioRxiv - Synthetic Biology 2019Quote: ... Sequence maps are included in File S2 and plasmids useful for constructing additional two-gene expression systems (pJS101 and pJS102, Table 1) are available from AddGene (deposit numbers 118280 and 118281) and have been verified by whole-plasmid sequencing [33].
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bioRxiv - Neuroscience 2020Quote: ... with two package plasmids: pCMV-VSV-G (Addgene, #8454) and pCMV-dR8.2 dvpr (Addgene ...
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bioRxiv - Genetics 2022Quote: ... TSCs were transfected with two PX458 plasmids (Addgene, #48138) each containing one single guide RNA (RE79_1/RE79_2 ...
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bioRxiv - Molecular Biology 2022Quote: ... two sgRNAs were cloned into the pCFD6 (Addgene #73915) vector which is a tRNA–sgRNA expression system using the endogenous tRNA processing machinery liberating multiple functional sgRNAs from a single precursor transcript in the nucleus29 ...
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bioRxiv - Systems Biology 2019Quote: For pPB - mCherry vector construction a PCR product encoding GCaMP5 sensor incorporating the CaMP3 mutation T302L R303P D380Y and no stop codon (Addgene plasmid #31788) was directionally ligated into pENTR/D-TOPO vector (Invitrogen K243520 ...
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bioRxiv - Bioengineering 2020Quote: ... The construction of PEgRNA transcript carrying plasmid: The empty PEgRNA plasmid was modified from the pgRNA-bacteria (ColE1 ori, Addgene plasmid #44251)30 by removing the 20 bp spacer ...
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bioRxiv - Synthetic Biology 2019Quote: The construction of the plasmid library is based on plasmids previously published: pJBL2801 was a gift from Julius Lucks (Addgene plasmid 71207) and was used for cloning the STARs and triggers ...
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bioRxiv - Neuroscience 2020Quote: ... we made two 100μL injections of AAVrg.CAG.tdTomato (Addgene, #59462-AAVrg) bilaterally into CFA (0.0 mm posterior ...
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bioRxiv - Neuroscience 2020Quote: ... we modified two plasmids procured from Addgene (#62724 and #74291). We digested plasmid #74291 with BamHI ...
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bioRxiv - Cancer Biology 2020Quote: ... Two crRNAs introduced into lentiviral vectors (pLentiCRISPR-E, Addgene #78852) which contains eSpCas9 and puroMYCin cassette.
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bioRxiv - Neuroscience 2021Quote: ... Kim modified two AAV vectors (Addgene plasmid #18917 and #38044). DNA of #18917 was cut with BamHI and Eco RI ...
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bioRxiv - Neuroscience 2020Quote: ... two unc-4 sgRNA plasmids and Cas9 plasmid (Addgene #46168) (Friedland et al. ...
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bioRxiv - Immunology 2019Quote: ... and the two Rictor shRNA vectors were purchased from Addgene. The pEnter/D-TOPO entry vector was purchased from Thermo Fischer ...
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bioRxiv - Immunology 2021Quote: ... Two sgRNA-containing pX330 plasmids (pSpCas9; Addgene, plasmid ID 42230) were constructed ...
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bioRxiv - Molecular Biology 2022Quote: Two plasmids (based on the gRNA expression plasmid Addgene #49330) encoding Cas9 as well as guide RNAs targeting the integration site were kindly received from the Brennecke Lab at IMBA Vienna (Batki et al ...