Labshake search
Citations for Addgene :
1 - 50 of 384 citations for Transcription Factor SOX 10 SOX10 Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
Comprehensive fitness landscape of SARS-CoV-2 Mpro reveals insights into viral resistance mechanismsbioRxiv - Molecular Biology 2022Quote: ... The LexA_ER_B112 transcription factor was amplified from Addgene_58437 (FRP880_PACT1(−1-520)-LexA-ER-haB112-TCYC1 was a gift from Joerg Stelling ...
-
bioRxiv - Cell Biology 2023Quote: ... fibroblasts were reprogrammed using lentiviral vectors that carried six transcription factors (ADDGENE/PSIN4-EF2-N2L ...
-
bioRxiv - Synthetic Biology 2023Quote: ... MS2-SoxS-LOVdeg was expressed from a variant of pJF093 (Addgene #113323). TetR and its corresponding promoter driving expression of MS2-SoxS were replaced with the LacI-PTrc inducible system from pBbA1c-mRFP1 from the BglBrick library (Lee et al. ...
-
bioRxiv - Neuroscience 2024Quote: The transcription factors and the reverse tetracycline transactivator (rtTA) were obtained from Addgene (Tet-O-Fuw-Ascl1 Addgene plasmid #27150 ...
-
bioRxiv - Genomics 2020Quote: pNTI729 was constructed by first amplifying the ZEM transcription factor from pNTI638 (pHES795 Addgene #87943) (17 ...
-
bioRxiv - Neuroscience 2021Quote: ... and the Egr1 transcription factor (pcDNA3-Egr1, a gift from Eileen Adamson, Addgene plasmid #11729) known to stimulate the Cav3.2 promotor 25.
-
bioRxiv - Cell Biology 2023Quote: ... fibroblasts were reprogrammed using lentiviral vectors that carried six transcription factors (ADDGENE/PSIN4-EF2-N2L, ADDGENE/PSIN4-EF2-O2S and ADDGENE/PSIN4-CMV-K2M) ...
-
bioRxiv - Developmental Biology 2020Quote: The plasmid sox10:mCherry (a gift from Thomas Look, Addgene plasmid # 98695) was injected in wild type or Da-/- medaka embryos at the one cell stage using I-SceI meganuclease protocols as previously described (Thermes et al. ...
-
Upregulated GIRK2 counteracts ethanol-induced changes in excitability & respiration in human neuronsbioRxiv - Neuroscience 2024Quote: ... NPCs were induced into neurons with doxycycline-inducible transcription factor NGN2 with neomycin antibiotic selection (Addgene #99378), following the protocol described by (Ho et al. ...
-
bioRxiv - Neuroscience 2020Quote: ... The lentiviral vector Teto-O-FUW-Sox10 (Addgene, MA, USA; Cat. No. 45843) and FUW-M2-rtTA (Addgene ...
-
bioRxiv - Molecular Biology 2024Quote: ... reporter cell lines were generated by TALEN-mediated homology-directed repair to integrate enhancer reporter donor constructs into the AAVS1 locus by electroporation of 1 × 106 K562 cells (or K562 cells selected to have the desired synthetic transcription factor) with 1 ng of reporter donor plasmid and 0.5 ng of each TALEN-L (Addgene no. 35431) and TALEN-R (Addgene no ...
-
bioRxiv - Synthetic Biology 2024Quote: ... Genes encoding natural transcription factors were sourced from: cJun (pCLXSN-c-JUN, which was a gift from Jin Chen, Addgene plasmid #102758)36 and BATF (pFUW-TetO-BATF ...
-
bioRxiv - Molecular Biology 2020Quote: ... At least two independent sets of oligonucleotide pairs for gene knockdown of human MTs and the transcription factor MTF-1 (supplementary table S4) were synthesized and cloned into the pLKO.1-TCR vector (Addgene, Cambridge, MA, USA); the same vector was also used as an empty vector control ...
-
bioRxiv - Cell Biology 2023Quote: ... fibroblasts were reprogrammed using lentiviral vectors that carried six transcription factors (ADDGENE/PSIN4-EF2-N2L, ADDGENE/PSIN4-EF2-O2S and ADDGENE/PSIN4-CMV-K2M). Fibroblast cells were plated in 6-well plates at 1.5 x 105 cells per well (Day 0) ...
-
bioRxiv - Developmental Biology 2022Quote: The three conditional lines for transcription factor overexpression (Rosa-A, GA, GAP) were constructed by modifying the Ai3 targeting construct (Addgene #22797; (Madisen, et al., 2010). The EGFP insert in Ai3 was removed by FseI digestion and replaced with coding regions for the following ...
-
bioRxiv - Bioengineering 2022Quote: ... The T-cell factor/lymphoid enhancer factor (TCF/LEF) luciferase reporter SuperTopFlash (STF) and the control pRL-SV40 Renilla luciferase constructs (Addgene) were used for Wnt/β-catenin-responsive reporter assays ...
-
bioRxiv - Genetics 2019Quote: ... Capped Cas9 mRNA was created by in vitro transcription using Thermo Fisher mMESSAGE mMACHINE™ SP6 Transcription Kit from pCS2-nls-zCas9-nls (Addgene#47929)
-
bioRxiv - Cancer Biology 2023Quote: ... transcription activators (lenti MS2-P65- HSF1_Hygro, Addgene plasmid #61426), and single guide RNA (sgRNA ...
-
bioRxiv - Genetics 2021Quote: ... to foster T7 in vitro transcription) for transcription from DR274 (DR274 was a gift from Keith Joung, Addgene plasmid #42250; Hwang et al., 2013).
-
bioRxiv - Cell Biology 2023Quote: ... Both GFP11-tagged Fluc proteins and GEM transcriptional factor (cloned from pJW1663, Addgene plasmid #112037) were stably integrated into yeast genome ...
-
bioRxiv - Genomics 2020Quote: ... sgRNA was synthesized via in vitro transcription applying plasmid pDD162 (Addgene, Cat # 47549) and HiScribe™ T7 Quick High Yield RNA Synthesis Kit (New England Biolabs ...
-
bioRxiv - Developmental Biology 2021Quote: ... the template for in vitro transcription was digested from pMLM3613 (Addgene catalogue #42251) with PmeI ...
-
bioRxiv - Developmental Biology 2022Quote: ... Cas9 mRNA was generated by linearisation and transcription from the PCS2-nCas9n (Addgene, #47929) clone ...
-
bioRxiv - Synthetic Biology 2019Quote: Plasmids encoding transcription templates were constructed using pUC19-T7-3WJdB-T (Addgene plasmid #87308) as a backbone ...
-
bioRxiv - Pharmacology and Toxicology 2023Quote: ... A human elongation factor 1 (EF1) promoter was introduced using a Gateway att4-att1 Entry clone (Addgene, Watertown, MA, #162920). Final expression clones were verified by restriction analysis and maxiprep DNA was prepared using the Qiaprep Maxiprep kit (Qiagen ...
-
bioRxiv - Neuroscience 2022Quote: ... the complementary DNA (cDNA) for each iMN factor (Ngn2, Lhx3, Isl1, NeuroD1, Ascl1, Brn2 and Myt1l) was purchased from Addgene and cloned into the pMXs retroviral expression vector using Gateway cloning technology (Invitrogen) ...
-
bioRxiv - Developmental Biology 2021Quote: ... CAS9 mRNA was obtained by in vitro transcription of linearised pT3TS-nCas9n vector (Addgene #46757) with MEGAscript T3 kit (Ambion) ...
-
bioRxiv - Immunology 2019Quote: ... RNAs were also generated from in vitro transcription of a modified p2RZ plasmid (Addgene #27664) where RNY4 or RNY4ds3 sequences were cloned between T7 promoter and HDV Ribozyme sequences.
-
bioRxiv - Bioengineering 2022Quote: ... The phiC31 integrase mRNA was prepared by in vitro transcription using EcoRl-linearized plasmid pCDNA3.1_phiC31 (Addgene #68310) or HindIII-linearized plasmid pT7_PhiC31o as the template and mMESSAGE mMACHINE™ T7 Transcription Kit (Invitrogen) ...
-
bioRxiv - Molecular Biology 2022Quote: Templates for in vitro transcription of SpCas9 guides were amplified from the plasmid pX330 (Addgene, Watertown, MA) using primer pairs with 65 oligonucleotides as a 5’ primer (forward ...
-
bioRxiv - Developmental Biology 2022Quote: ... The Cas9 mRNA and sgRNA were produced by in vitro transcription from pCS2-nCas9n (Addgene Plasmid #47929) and DraI-digested pDR274 (Addgene Plasmid #42250 ...
-
bioRxiv - Biophysics 2023Quote: ... RNA samples were prepared by in vitro transcription (IVT) using T7 RNA polymerase (Addgene #12413848, prepared in-house). T7 RNA Polymerase ...
-
bioRxiv - Neuroscience 2022Quote: ... the NheI – BsrGI fragment containing SFL in the pcDNA3.1/CAG vector was ligated into the corresponding RE sites of an AAV2 vector with the elongation factor 1α promoter and double-floxed inverted open reading frame (EF1a-DIO; a gift from Karl Deisseroth; Addgene plasmid #: 55631; RRID: Addgene_55631) in the untranslatable reverse orientation ...
-
bioRxiv - Genomics 2023Quote: ... 10 μg payload DNA and 10 μg pCAG-iCre plasmid (Addgene #89573) per transfection ...
-
bioRxiv - Cell Biology 2022Quote: ... GFP1-10 plasmid (pHAGE2-EF1a-GFP1-10-IRES-Puro) was generated by cloning GFP1-10 from pcDNA3.1-GFP(1-10) (Addgene Plasmid #70219) into a lentiviral vector that contains EF-1α promoter and Puromycin selection marker ...
-
bioRxiv - Cell Biology 2023Quote: ... 5*10^10 genomic copies of commercially produced AAV8-TBG-Cre (Addgene #107787) or control AAV8-TBG-GFP (Addgene #105535 ...
-
bioRxiv - Neuroscience 2021Quote: ... The sgRNA to target exon 2 of Ptrpq was generated by in vitro transcription of the target sequence cloned downstream of the T7 promoter in the pX330 vector (Addgene). In vitro transcription was performed using the MAXIscript T7 kit (Life Technologies ...
-
bioRxiv - Genetics 2023Quote: ... following the manufacturer’s protocol with AAVS1 targeting vector and predesigned transcription activator-like effector nucleases (hAAVS1 TALEN Left and Right were gifts from Su-Chun Zhang, Addgene plasmid # 52341 & 52342 ...
-
bioRxiv - Neuroscience 2022Quote: ... 10 μL of AAV-PHPeB-hsyn-cre-eGFP (Addgene#105540, titer ∼1×10^13) or AAV-PHPeB-CAG-GFP (Addgene#37825 ...
-
bioRxiv - Immunology 2023Quote: ... 10 μg pMD2.G (Addgene) and 100 μL lipofectamine 2000 (Thermo Fisher) ...
-
bioRxiv - Cell Biology 2023Quote: ... AICSDP-10:LMNB1-mEGFP (Addgene plasmid #87422 ...
-
bioRxiv - Cancer Biology 2023Quote: ... 10 μg pMD2.G (Addgene) envelope plasmid and 130 μL 1 μg / mL polyethylenimine (Sigma-Aldrich ...
-
bioRxiv - Cell Biology 2023Quote: ... pHAGEmCherry-LC3B (Addgene 201924 10).
-
bioRxiv - Cell Biology 2020Quote: DNA templates used for in vitro transcription of mouse Xist RNA were amplified from sequence verified plasmid pCMV-Xist-PA (Wutz and Jaenisch, 2000) (Addgene 26760), containing the murine Xist gene using high-fidelity Platinum® pfx DNA polymerase (Invitrogen™) ...
-
bioRxiv - Evolutionary Biology 2019Quote: ... Cas9 mRNA was obtained by in vitro transcription of linearized pT3TS-nCas9n plasmid (a gift from Wenbiao Chen (Addgene plasmid #46757)) using the mMESSAGE mMACHINE T3 kit (Ambion ...
-
bioRxiv - Cancer Biology 2023Quote: ... we replaced the VP64-P65-Rta sequence (encoding transcription activator complex) in the lentiviral vector of dCas9-VPR-mCherry fusion protein (for CRISPRa, Addgene #102245) with the KRAB-MECP2 sequence from the transient expression vector of dCas9-KRAB-MECP2 (for CRISPRi ...
-
bioRxiv - Neuroscience 2022Quote: ... AAV9-EF1a-DIO-hChR2(H134R)-EYFP (Addgene, 1.8*10^13 gc/ml, diluted 1:10), AAV2-hSyn-hChR2(H134R)-EYFP (UNC Vector Core ...
-
bioRxiv - Neuroscience 2019Quote: ... AAV5 CaMKII-hM3Dq-IRES-mCitrine (3.75×10^14 or 3.75×10^13 virus molecules/mL) (Addgene plasmid #50466 ...
-
bioRxiv - Cell Biology 2023Quote: ... and (3) GFP1-10 from pFA6a-link-yGFP1-10-CaURA3MX (Addgene 86419; (Smoyer et al., 2016)) and subsequent cloning into the XhoI/NotI sites of pRS304 mito-DsRed.
-
bioRxiv - Cell Biology 2019Quote: ... and the sequence follows 5’-atgagtcggtctggcaaccaggtgtcggagtacatctcaaacacatttctcgataagcaacatgaagtggaaatcccctctccaacgcagaaggaaaaagaggaggaggaagagccgatgtcgcagatcagtggggtcaagaagttgatgcacagctccagcctaactaattcatgtatc-3’ Templates for in vitro transcription of the sgRNAs were PCR amplified from the pX335 plasmid (a gift from Feng Zhang, Addgene plasmid # 42335) using the following primers ...