Labshake search
Citations for Addgene :
1 - 32 of 32 citations for Tetracycline since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2020Quote: ... tetracycline induced cas9 vector (Addgene # 50661) was stably expressed in prostate cancer lines ...
-
Multi-omics characterization of partial chemical reprogramming reveals evidence of cell rejuvenationbioRxiv - Molecular Biology 2023Quote: ... or the reverse tetracycline transactivator36 (Addgene #20342), were encapsulated in PureFection nanoparticles (System Biosciences ...
-
bioRxiv - Neuroscience 2020Quote: ... and reverse tetracycline-controlled transactivator rtTA (Addgene, 66810) lentivirus with 8 ug/mL polybrene (Sigma ...
-
bioRxiv - Molecular Biology 2022Quote: K562 cells stably expressing the reverse tetracycline transactivator (RTTA - Addgene 26429) were nucleofected using a modified LMNB1 donor expressing mCherry under a Tet promoter (PCR 2070) ...
-
bioRxiv - Neuroscience 2023Quote: ... with the leaky tetracycline response element from pAAV-FLEX-hGTB (Addgene 26196) followed by a tricistronic open reading frame consisting of the genes for the “improved tetracycline transactivator” itTA82 ...
-
bioRxiv - Neuroscience 2023Quote: We obtained plasmids of reverse tetracycline-controlled transactivator (rtTA) (FUdeltaGW-rtTA; Addgene, #19780) and NGN2 (pTet-O-Ngn2-puro ...
-
bioRxiv - Neuroscience 2024Quote: The transcription factors and the reverse tetracycline transactivator (rtTA) were obtained from Addgene (Tet-O-Fuw-Ascl1 Addgene plasmid #27150 ...
-
bioRxiv - Molecular Biology 2024Quote: Neurogenin 2 (NGN2) was expressed in iPSC lines using a tetracycline inducible promoter (Addgene 172115) integrated using Piggybac plasmid EFa1-Transposase (Addgene plasmid 172116 ...
-
bioRxiv - Cancer Biology 2019Quote: ... WT or Mutant FGFR2 IIIb ORFs were cloned into pInducer20 Tetracycline-inducible lentiviral vector (Addgene #44012) using gateway cloning technology (Invitrogen #11791019) ...
-
bioRxiv - Plant Biology 2023Quote: ... and E04 were transformed with tetracycline (Tet) resistant plasmids pSW002-PpsbA-DsRed-Express2 (DsRed; Addgene, 111257) via electroporation (Supplemental Methods S1) ...
-
bioRxiv - Cancer Biology 2021Quote: ... the tetracycline inducible expression construct was PCR-amplified from pCW57.1 (a gift from David Root, Addgene # 41393) and the H2B-GFP reporter was PCR-amplified from LV-GFP (a gift from Elaine Fuchs ...
-
bioRxiv - Cell Biology 2022Quote: ... and the constitutive tetracycline repressor A3 mutant expressing pLenti-CMV-rtTA3 (gift from Eric Campau (Addgene plasmid # 26429) to infect AoSMC cells as described in (Kubben et al. ...
-
bioRxiv - Microbiology 2021Quote: ... Switzerland) and mStrawberry were stably expressed in hBMECs under Tetracycline inducible promoter in pLKO-Puro-tet plasmid (Addgene, 21915). All transfections were performed using Lipofectamine 3000 reagent (Invitrogen ...
-
bioRxiv - Molecular Biology 2023Quote: ... 2435696-24478046) was replaced by the tetracycline resistant gene from the PfoI/EcoRI fragment of pRGD-TcR (Addgene, #74110) (Ledermann et al ...
-
bioRxiv - Cancer Biology 2021Quote: ... HT-1080N cells were transduced with lentiviruses carrying the reverse tetracycline-controlled transactivator 3 under a CMV promoter (CMV-rtTA3) (Cat# w756-1, Addgene). HT-1080N-rtTA3 cell lines were selected in 10 μg/mL blasticidin (Cat # A1113902 ...
-
bioRxiv - Cell Biology 2021Quote: ... and pLenti CMV rtTA3 Hygro (w785-1) used for creating tetracycline-inducible cell lines were gifts from Eric Campeau (w762-1: Addgene plasmid #26434 ...
-
bioRxiv - Genomics 2019Quote: Melanoma cells and melanocyte growth assays were conducted using lentiviral transduction of MX2 cDNA under the control of tetracycline-inducible promoter using pINDUCER20 vector (Addgene). The MX2 cDNA clone (RC206437 ...
-
bioRxiv - Neuroscience 2022Quote: ... hiPSCs were transduced with a lentivirus containing the NGN2-gene under tetracycline-inducible promotor (Tet-O-Ngn2-Puro) in combination with lentivirus carrying FUdeltaGW-rtTA construct (both plasmids from Addgene, lentivirus packing and concentration by Alstem ...
-
bioRxiv - Genetics 2023Quote: ... in an all-in-one tetracycline- inducible expression cassette with AAVS1 homology arms (AAVS1-TRE3G-EGFP was a gift from Su-Chun Zhang (Addgene plasmid # 52343 ...
-
bioRxiv - Cell Biology 2023Quote: ... Tetracycline-on (Tet-on) cells were generated by lentiviral transduction with a pCW57.1 vector (Addgene plasmid 41393, David Root lab) containing a single-vector Tet-on component and were cultured in the presence of 1 µg/ml doxycycline (Clontech ...
-
bioRxiv - Biophysics 2023Quote: Fusion constructs with pTREtight2 vectors were co-transfected with reverse tetracycline-controlled transactivator 3 (pLenti CMV rtTA3 Hygro (w785-1)) plasmid (Addgene) in the presence of doxycycline (Clontech) ...
-
bioRxiv - Molecular Biology 2021Quote: The doxycycline inducible polycistronic reprogramming vector was generated by cloning a GFP-IRES sequence downstream of the tetracycline response element in the backbone FUW-tetO-hOKMS (Addgene 51543). This vector was used in combination with FUW-M2rtTA (Addgene 20342) ...
-
bioRxiv - Microbiology 2022Quote: HK1 cells that stably express GFP-LMP1 under control of a tetracycline-inducible promoter were created by first being transduced with lentivirus particles containing pLenti CMV TetR BLAST (Addgene; 17492) as previously described in (18) ...
-
bioRxiv - Developmental Biology 2020Quote: MEFs that were homozygous for reverse tetracycline transactivator (rtTA) allele at the Rosa26 locus were transduced with Tet-inducible Brn2 (Addgene 27151), Ascl1 (Addgene 27150) ...
-
bioRxiv - Bioengineering 2022Quote: The lentiviral transfer plasmid with constitutively expressed tetracycline transactivator (pEF1α-rtTA-Antares-WPRE) was constructed as follows: pNCS-Antares was obtained from Addgene (#74279) and P2A was added to the N-terminus of Antares with a primer overhang during PCR ...
-
bioRxiv - Developmental Biology 2022Quote: MEFs that were homozygous for reverse tetracycline transactivator (rtTA) allele at the Rosa26 locus were transduced with Tet-inducible Brn2 (Addgene, 27151), Ascl1 (Addgene ...
-
bioRxiv - Cancer Biology 2023Quote: ... the EF-1α core promoter and the tetracycline repressor (TetR) into the KpnI and BamHI sites of lentiCRISPR-v2 (Addgene, 52961). Then ...
-
bioRxiv - Molecular Biology 2023Quote: ... the complete CMV promoter was replaced with the tetracycline- responsive PTight promoter from pCW57.1-MAT2A (a gift from David Sabatini; Addgene plasmid # 100521). Second ...
-
bioRxiv - Microbiology 2021Quote: Cells stably expressing shRNA of different genes under the control of a tetracycline-inducible promoter were created by first transducing SNB-19 cells with lentivirus particles containing pLenti CMV TetR BLAST (Addgene; number 17492). The cells underwent selection with media containing 10 μg/mL of blasticidin (Invivogen ...
-
bioRxiv - Cell Biology 2020Quote: ... targeting KIF4A sequence 5’-GCAAGATCCTGAAAGAGAT-3’ was generated using a multipurpose GATEWAY-based lentiviral tetracycline-regulated conditional RNAi system (GLTR) using pENTR-THT-III (Addgene plasmid #55791) and pGLTR-X-Puro (Addgene plasmid #58246 ...
-
bioRxiv - Neuroscience 2023Quote: ... LRP10splice was subcloned from the pLVX-EF1α-LRP10splice-IRES-NeoR plasmid via PCR and placed after the rtTA (reverse tetracycline-controlled transactivator)-Advanced promoter in the AgeI-digested pCW57-MCS1-2A-MCS2 (Addgene, 71782, from Adam Karpf) plasmid via Gibson Assembly® ...
-
bioRxiv - Neuroscience 2023Quote: ... LRP10 was subcloned from the pLVX-EF1α-LRP10-IRES-NeoR plasmid and placed after the rtTA (reverse tetracycline-controlled transactivator)-Advanced promoter in the AgeI-digested pCW57-MCS1-2A-MCS2 (Addgene, 71782, from Adam Karpf) plasmid via Gibson Assembly® ...