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Citations for Addgene :
1 - 50 of 838 citations for Tetanus Toxoid Recombinant Heavy Chain Fragment C since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: Tetanus toxin light chain (TeTxLC) was amplified by PCR from pGEMTEZ-TeTxLC (Addgene #32640, (Yu et al., 2004)) using forward primer ...
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bioRxiv - Immunology 2023Quote: ... Amplicon from the first PCR reaction were used as templates for cloning into antibody expression vectors (Abvec2.0-IGHG1 for the heavy chain and Abvec1.1-IGKC or Abvec1.1.-IGLC2-XhoI for of the light chains, all from AddGene).
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bioRxiv - Bioengineering 2024Quote: ... pcDNA3.1-Tras heavy chain-SpyTag003 (‘Tras NoLink’) and pcDNA3.1-Tras light chain (GenBank and Addgene deposition in progress) were cloned previously by Jamie Lam (University of Oxford ...
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bioRxiv - Physiology 2021Quote: ... The alpha myosin heavy chain/puro rex/neo was a gift from Mark Mercola (Addgene plasmid #21230 ...
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bioRxiv - Neuroscience 2023Quote: ... Tetanus Neurotoxin Light Chain (TeLC) viruses were generated using AAV5-hSyn-FLEX-TeLC-P2A-dTomato (Addgene, 159102, 1 × 1013 genome copies per ml) at ISTA viral facility ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting chimera genes for the light chain and the His-tagged heavy chain of the Fabs were separately cloned into the pCAGEN vector (a gift from Connie Cepko (Addgene plasmid #11160)) 34 ...
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bioRxiv - Immunology 2023Quote: ... Variable regions were cloned into pVITRO1 plasmids that already contained the constant regions for human IgG1 heavy and light chains (κ or λ, Addgene plasmids #61883 and #50366)85 using Gibson Assembly75 ...
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bioRxiv - Microbiology 2022Quote: ... with a 15 base pair overlap with vector backbone were used in a polymerase chain reaction (PCR) to generate fast Timer protein timer fragment from plasmid pFast-FT-N1 (Addgene 31910). Five tandem repeats of Tax responsive element (TRE ...
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bioRxiv - Physiology 2023Quote: ... with the carboxy tail fused to either the N-fragment (VN) or the C-fragment (VC) of the Venus protein (27097, 22011; Addgene, Cambridge, MA), auxiliary subunits CaVα2δ ...
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bioRxiv - Genomics 2019Quote: ... The mCherry-OCT4-AID fragment was cloned into the pLEX_305-C-dTAG backbone (Addgene #91798) (Nabet et al. ...
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bioRxiv - Biochemistry 2020Quote: Recombinant wild-type and mutant SpCas9 (1-1,368) possessing a C-terminal decahistidine tag (Addgene, no. 62731) was expressed and purified as described previously (35) ...
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bioRxiv - Cell Biology 2023Quote: cDNA sequences encoding N- and C-terminal fragments of Venus were amplified from pCe-BiFC-VN173 (Addgene) and pCe-BiFC-VC155 (Addgene ...
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bioRxiv - Cancer Biology 2021Quote: ... and single-chain gRNA encoding plasmid (MLM3636, AddGene #43860) were gifts from Keith Joung ...
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bioRxiv - Cell Biology 2021Quote: ... Single-chain antibodies fused to GFP (scAB-GFP, Addgene #104998) were used for imaging translation sites through nascent polypeptide labeling ...
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bioRxiv - Cell Biology 2022Quote: ... The mammalian expression plasmid for Ism1 with C-terminal myc-6xhis tag plasmid for recombinant Ism1 protein production was from Addgene (#173046).
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bioRxiv - Biochemistry 2022Quote: ... For expression of recombinant drosophila c-Src isoform 42A (Src42A, UniProtKB Q9V9J3) we used a pET-His10-Src42A plasmid (Addgene #126674) codifying a full-length drosophila isoform (aa 1-517 ...
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bioRxiv - Cell Biology 2023Quote: Plasmids encoding recombinant WT (RRID:Addgene_92100) and M880A mutant human GST-TAF3-PHD were kindly provided by Albert Jeltsch (Kungulovski et al. ...
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bioRxiv - Genetics 2022Quote: ... ALKBH5 fragments (Addgene, 134783) or dALKBH5 fragments (Addgene ...
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bioRxiv - Molecular Biology 2022Quote: ... The fragments are then cloned into a mammalian expression vector containing Flag and mEGFP (N- or C-terminal) (modified from Addgene #32104) using NEBuilder HiFi DNA Assembly kit (E2611) ...
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bioRxiv - Genetics 2023Quote: ... containing the sgRNA-EGxxFP reporter target sequence was placed between the N and C parts of the EGFP fragment of pCX-EGxxFP [25] (Addgene plasmid #50716). PGK>HSV-TK was then inserted downstream of the CAG>EGxxFP reporter ...
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bioRxiv - Neuroscience 2019Quote: ... recombinant mouse E1 (Addgene plasmid # 32534), E2 (pGEX4T-1-UbcH5b) ...
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bioRxiv - Biochemistry 2024Quote: Recombinant human GST-CK2α’ (Addgene #27084) and recombinant human GST-CK2α (Addgene #27083 ...
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bioRxiv - Genetics 2022Quote: ... or dALKBH5 fragments (Addgene, 134784) and backbones (Addgene ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were transfected with mCherry – clathrin light chain B (Addgene Plasmid #55019) and EGFP – clathrin light chain A52 plasmids ...
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bioRxiv - Microbiology 2020Quote: ... Recombinant strains harbouring pTEC19 plasmid (Addgene, #30178) and producing the fluorescent protein E2-Crimson were grown in Middlebrook 7H9 broth supplemented with 0.2% glycerol (v/v ...
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bioRxiv - Biochemistry 2023Quote: ... and YFP tagged recombinant proteins (Addgene #173080), genes were inserted between N-terminal 6x His-tag followed by CFP/YFP tag and a TEV protease cleavage site of pNIC28-Bsa4 ...
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bioRxiv - Biochemistry 2024Quote: ... and recombinant human GST-CK2α (Addgene #27083) enzymes were produced in-house as previously described [30] ...
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bioRxiv - Molecular Biology 2023Quote: ... RBM41 C-terminal fragment (259–413) and full-length 65K were cloned into MAC-tag-N vector (Addgene #108078, a gift from Markku Varjosalo) using Gateway cloning as described (Liu et al. ...
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bioRxiv - Molecular Biology 2022Quote: PCR fragment with pY010 (Addgene #69982) as template and the following primers was cloned into pX458 (Addgene # 48138 ...
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bioRxiv - Neuroscience 2023Quote: The loxpSTOPlox (LSL) fragment from Addgene #89587 was amplified with primers CAG-LSL-MreI-F and CAG-LSL-EcoRV-R ...
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bioRxiv - Developmental Biology 2021Quote: A mouse Cnr1 cDNA fragment (Addgene, #13391) was cloned into all-in-one tetracycline inducible plasmid (pAS4.1w.Ppuro-aON ...
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bioRxiv - Bioengineering 2021Quote: ... Fragments of PE2-encoding sequence (Addgene #132775) and NG-Cas9-encoding sequence (Addgene #124163 ...
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bioRxiv - Genomics 2021Quote: ... gene fragment containing CTT pegRNA (Addgene #132778) was PCR amplified using primer sets adding 5-bp degenerate barcode and flanking BsmBI site for the downstream cloning steps ...
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bioRxiv - Cancer Biology 2023Quote: ... the fragment was ligated into tRMPVIR (Addgene) plasmid ...
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bioRxiv - Cancer Biology 2023Quote: ... the fragment was ligated into pENTR2B (Addgene) and then recombined into pCW57.1 (Addgene) ...
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bioRxiv - Genetics 2021Quote: ... This fragment was used to replace the NdeI/EcoRI fragment from pCMV-BE2 (Addgene#73020 (Komor et al., 2016)) digested with NdeI and EcoRI using a 3-way ligation reaction (Rapid DNA ligation kit ...
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bioRxiv - Microbiology 2021Quote: ... The recombinant plasmid containing human ACE2 gene (Addgene #1786) was transfected into HEK293T cells using Lipofectamine 2000 Transfection Reagent (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: Full-length recombinant human WT α-syn (Addgene #213498), α-syn 1-95 (Addgene #213499) ...
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bioRxiv - Bioengineering 2020Quote: ... The BE3-blasticidin fragment was obtained by assembly of the BE3 fragment amplified from the pCMV-BE3 vector (Addgene #73021) and the blasticidin fragment amplified from lentiCas9-Blast (Addgene #52962) ...
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bioRxiv - Cell Biology 2021Quote: ... by replacing a fragment of pDD282 (Addgene #66823) containing the GFP sequence with a similar fragment containing a codon optimized mCherry sequence with synthetic introns using the flanking Bsu36I and BglII restriction sites ...
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bioRxiv - Cell Biology 2021Quote: ... The SEC_3xFLAG_ccdB fragment was from pDD282 (Addgene #66823), except that the let-858 3’UTR was substituted by the par-5 3’UTR to prevent gene silencing in the germline ...
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bioRxiv - Microbiology 2022Quote: ... PCR fragments amplified from pFLAG-attP (Addgene, #110095) with primers Apr fw/Apr rv and from pSEVA524 with primers tetA fw/tetA rv,25 respectively ...
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bioRxiv - Molecular Biology 2021Quote: ... and opie2-dsRed marker fragment amplified from Addgene plasmid #100580 using primers 1010.C7 and 1010.C8 ...
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bioRxiv - Molecular Biology 2021Quote: ... a p10 3’UTR fragment amplified from Addgene plasmid #100580 with primers 1010.C5 and 1010.C6 ...
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bioRxiv - Cell Biology 2020Quote: ... by replacing a fragment of pDD282 (Addgene #66823) comprising the GFP sequence with a similar fragment comprising a codon optimized and synthetic intron-containing eGFP sequence using the flanking Bsu36I and BglII restriction sites ...
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bioRxiv - Biochemistry 2021Quote: ... Gene fragments were cloned into pNIC28-Bsa4 (Addgene #26103 ...
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bioRxiv - Bioengineering 2020Quote: ... a p10 3’UTR fragment amplified from Addgene plasmid #100580 19 with primers 986.C3 and 986.C4 ...
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bioRxiv - Bioengineering 2020Quote: ... and an sgRNA scaffold fragment amplified from Addgene plasmid #49411 23 with primers 1045.C3 and 1045.C4 ...
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bioRxiv - Cell Biology 2020Quote: ... A fragment spanning the pMSCV-hygro (Addgene, 634401) Hygromycin B resistance cassette was amplified by PCR (primer sequences available upon request ...
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bioRxiv - Cell Biology 2023Quote: ... Resulting fragments and mCherry cDNA amplified from Addgene plasmid #102245 were assembled using Gibson Assembly ...